Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Characterization of Azomonas agilis PY101, a Cadmium-Resistant Strain Isolated from Anyang Stream

  • You, Kyung-Man (Department of Biology, Korea University) ;
  • Lee, Ji-Hyun (Department of Biology, Korea University) ;
  • Kim, Jeong-Kook (Department of Biology, Korea University) ;
  • Hah, Nam-Ju (Department of Pharmacy, Sahmyook University) ;
  • Lee, Yung-Nok (Department of Biology, Korea University) ;
  • Park, Yong-Keun (Department of Biology, Korea University, Graduate School of Biotechnology, Korea University)
  • Published : 1996.09.01
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Abstract

A cadimium-resistant strain isolated from Anyang stream, Azomonas agilis PY101 exhibited strong resistance to 1000 ppm of cadmium ion $(Cd^{2+})$. A agilis PY101 also exhibited resistance to various antibiotics, such as amoxicillin, amplicillin, bacitracin, cefazolin, erythromycin, penicillin, tetracycline, and vancomycin. In the presence of $Cd^{2+}$, the growth of A. agilis PY101 started after an extended lag phase and produced a green-fluorescent pigment induced by cadmium. The dramatic decrease (approximately 400ppm) of concentration of $cd^{2+}$ in the culture medium during the growth phase of A. agilis PY101 was confirmed by the inductively coupled plasma-atomic emission spectrophotometer. Transmission electron microscopic analysis revealed that A. agilis PY 101 actively accumulated $Cd^{2+}$ in the cytoplasm.

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References

  1. Metal ions and bacteria Beveridge, T.J.;R.J. Doyle
  2. Anlimicrob. Agents. Chemother v.7 Mechanism of plasmid-meiated resistance to cadmium in Staphylococcus aureus Chopra, I.
  3. Appl. Environ. Microbiol. v.59 Precipitation of cadmium by Clostridium thermoaceticum Daryl, P.C.;L. L. Lundie
  4. Science v.225 Cadmium-Resistant Pseudomonas putida synthesized Novel cadmium proteins Denise, P.H.;Peter, J.S.
  5. J. Bacteriol. v.171 Plasmid-determined inducible efflux is responsible for resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus Dietrich, H.N.;S. Silver
  6. FEMS Microbiology Let. v.11 Toxicity of heavy metals to soil bacteria Duxbry, T.
  7. Appl. Microbiol. v.15 Copper sulfide precipitation by yeasts from acid mine-waters Ehrlich, H.L.;S.I. Fox
  8. Ann. Rev. Microbiol. v.40 Microorganisms in reclamation of metal Herchins, S.R.;M.S. Davidson;J.A. Brierley;C.L. Brierley
  9. Bergey's manual of determinative bacteriology Holt, J.G.;N.R. Krieg;P. Sneath;J.T. Staley;S. T. Williams
  10. Trends Biochem. Sci. v.3 Metallothionein Kojima, Y.;J. H. R. Kagi
  11. J. Bacterial. v.117 Mercury and cadmium resistances mediated by the penicillinase plasmid in Staphylococcus aureus Kondo, I.;T. Ishikawa;H. Nakahara
  12. J. Bacteriol. v.162 Cadmium-resistant mutant of Bacillus subtilis 168 with reduced cadmium transport Laddaga, R. A.;R. Bessen;S. Silver
  13. J. Gen. Microbiol. v.130 Cadmium accumulation by a Cilrobacter sp Macaskie, L. E.;A. C. R. Dean
  14. J. Bacteriol. v.133 Single-strand breakage in DNA of Escherichia coli exposed to $Cd^{2+}$ Mitra, R. S.;I. A. Bernstein
  15. Nature v.266 Frequency of heavy-metal resistance in bacteria from inpatients in Japen Nakahara, H.;T. Ishikawa;Y. Sarai;I. Kondo
  16. J. Bacteriol. v.95 Plasmid-linked resistance to inorganic salts in Staphylococcus aureus Novick, R.P.;C. Roth
  17. Microbiol. Rev. v.56 Gene regulation of plasmid- and chromosome-determined inorganic ion transport in bacteria Simon, S.;M. Walderhaug
  18. Annu. Rev. Microbiol. v.32 Microbial transformations of metals Summers, A.O.;S. Silver
  19. FEMS Microbiol. Rev. v.32 Metal resistance in bacteria Trevors, J.T.;K.M. Oddie;B.H. Belliveau
  20. Acta Microbiol. Pol. v.7 Plasmid dependent impermeability barrier to cadmium ions in Staphylococcus aureus Tyneck, A.Z.;J. Zajac;Z. Gos
  21. J. Bacteriol. v.147 Energy-dependent efflux of cadmium coded by a plasmid resistance determinant in Staphylococcus aureus Tyneck, A. Z.;Z. Gos;J. Zajac