• 대한의생명과학회지
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• 제4권2호
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• pp.137-142
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• 1998

쥐 뇌에서 발현되는 S-100 beta 유전자의 다형현상을 조사하였다. Polymerase chain reaction을 위한 주형으로는 뇌에서 분리한 mRNA를 직접 역전사한 cDNA, 또는 rat brain cDNA library에서 분리한 phage DNA를 사용하였다. 증폭된 DNA 절편들은 크기가 기존에 보고되었던 것과 일치하였으나 DNA sequencing을 통한 세부적인 분석 결과 coding region 내에 염기변화 (CAT가 CAC로 변함)가 있음이 확인되었다. 그러나 이들은 모두 histidine을 결정하는 유전암호이기에 단백질의 1차구조에는 아무런 영향을 미치지 않는 다형현상으로 결론지어졌다. 본 연구는 S-100 beta 단백질에 그동안 알려지지 않았던 다형현상이 존재함을 시사하는 것으로서 S-100beta 효소 유전자의 전체적인 구조를 이해하기 위한 학문적 자료가 될 것으로 기대된다.

• Journal of Pharmaceutical Investigation
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• 제31권1호
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• pp.7-12
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• 2001

Amphotericin B (AmB) is a drug of choice for the treatment of systemic fungal diseases, but its use is considerably limited due to a high incidence of toxicity, particularly nephrotoxicity. It has been demonstrated that the toxicity of AmB is caused by self-aggregated species of the drug and that unaggregated (monomeric) drug is nontoxic but still expresses antifungal activity. Poly (ethylene oxide) (PEO) is a water-soluble polymer, which may impact the aggregation state of AmB. We have studied the aggregation state of AmB as a function of PEO molecular weight and concentration. At 3,000 and 8,000 g/mole, there was minimal or no change of critical aggregation concentration (CAC) of AmB regardless of the concentration of polymer. By contrast at 20,000 g/mole, the CAC of AmB strikingly increased to 24.3 and $37.5\;{\mu}M$ at 5.0% and 10 % w/v of polymer, respectively. The critical overlap concentration (COC) of PEO 20,000 g/mole was 5.5%. It appears that an interaction between monomeric AmB and polymer coil increases above the COC, competing with self-aggregation of the drug. Accordingly, the degree of aggregation of AmB stayed low and the toxicity became less. There was no such effect at 3,000 and 8,000 g/mole of PEO, owing perhaps to small dimensions in comparison to AmB. Based upon these findings, less toxic AmB formulation may be developed by a pharmaceutical technique such as solid dispersion system containing both AmB and PEO 20,000 g/mole.

• Bulletin of the Korean Chemical Society
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• 제22권5호
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• pp.467-475
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• 2001

A triblock copolymer based on $poly(\varepsilon-caprolactone)$ (PCL) as the hydrophobic part and poly(ethylene glycol) (PEG) as the hydrophilic portion was synthesized by a ring-opening mechanism of ${\varepsilon}-caprolactone$ with PEG containing a hydroxyl group at bot h ends as an initiator. The synthesized block copolymers of PCL/PEG/PCL (CEC) were confirmed and characterized using various analysis equipment such as 1H NMR, DSC, FT-IR, and WAXD. Core-shell type nanoparticles of CEC triblock copolymers were prepared using a dialysis technique to estimate their potential as a colloidal drug carrier using a hydrophobic drug. From the results of particle size analysis and transmission electron microscopy, the particle size of CEC core-shell type nanoparticles was determined to be about 20-60 nm with a spherical shape. Since CEC block copolymer nanoparticles have a core-shell type micellar structure and small particle size similar to polymeric micelles, CEC block copolymer can self-associate at certain concentrations and the critical association concentration (CAC) was able to be determined by fluorescence probe techniques. The CAC values of the CEC block copolymers were dependent on the PCL block length. In addition, drug loading contents were dependent on the PCL block length: the larger the PCL block length, the higher the drug loading content. Drug release from CEC core-shell type nanoparticles showed an initial burst release for the first 12 hrs followed by pseudo-zero order release kinetics for 2 or 3 days. CEC-2 block copolymer core-shell type nanoparticles were degraded very slowly, suggesting that the drug release kinetics were governed by a diffusion mechanism rather than a degradation mechanism irrelevant to the CEC block copolymer composition.

• Asian-Australasian Journal of Animal Sciences
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• 제14권12호
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• pp.1734-1740
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• 2001

Feeding trials using weanling pigs and broiler chicks were conducted to evaluate the efficacy of different metal-amino acid chelates and complexes at various levels of copper and zinc on the performance and fecal excretions. A total of 200 weanling pigs (Large White ${\times}$ Yorkshire ${\times}$ Duroc, $11.20{\pm}0.81kg$) were randomly assigned to 5 dietary treatments following a randomized complete block design. Each treatment was replicated 4 times with 10 pigs per pen. The dietary treatments were designated as : A-diet containing 170 ppm Cu from $CuSO_4$ and 120 ppm Zn from $ZnSO_4$, B-diet containing 85 ppm Cu from Cu-amino acid chelate (CAC) and 60 ppm Zn from Zn-amino acid chelate (ZAC), C-diet containing 170 ppm Cu from CAC and 120 ppm Zn from ZAC, D-diet containing 85 ppm Cu from Cu-lysine complex (CL) and 60 ppm Zn from Zn-methionine complex (ZM), and E-diet containing 170 ppm Cu from CL and 120 ppm Zn from ZM. On the other trial, 144 of one day old broiler chicks were randomly distributed to 6 dietary treatments following a completely randomized design. Each treatment was replicated 3 times with 8 chicks per replicate. The dietary treatments were as follows: 1-diet with 60 ppm Cu from $CuSO_4$ and 40 ppm Zn from $ZnSO_4$, 2-diet with 120 ppm Cu from $CuSO_4$ and 80 ppm Zn from $ZnSO_4$, 3-diet with 60 ppm Cu from CAC and 40 ppm Zn from ZAC, 4-diet with 120 ppm Cu from CAC and 80 ppm Zn from ZAC, 5-diet with 60 ppm Cu from CL and 40 ppm Zn from ZM, and 6-diet with 120 ppm Cu from CL and 80 ppm Zn from ZM. In Exp. 1 with pigs, there was no difference on average daily gain and average daily feed intake observed among treatments. There was improvement (p<0.05) on the overall feed conversion ratio (FCR) of pigs fed diet containing 120 ppm Zn and 170 ppm Cu from metal-amino acid chelates relative to those fed diet containing inorganic sources of Cu and Zn but equally efficient as those fed diet containing metal-amino acid complexes. Pigs fed diet containing either metal-amino acid chelates or complexes as sources of Cu and Zn had higher (p<0.05) Cu and Zn concentration in serum and lower (p<0.05) in feces than those receiving diet with inorganic sources. In Exp. 2 with broiler chicks, the overall FCR was not different among treatments. Higher (p<0.05) Cu and Zn concentration in serum was obtained from birds fed diet with 60 ppm Cu and 40 ppm Zn from metal-amino acid chelates compared to those fed diet with inorganic sources of Cu and Zn. Also, the feces collected from birds fed diet with either metal-amino acid chelates or complexes contained generally lower Cu and Zn than those birds fed diet with inorganic sources. The higher the dietary level of Cu and Zn the higher the Cu and Zn concentration in the feces. Based on the results, both metal-amino acid chelates and complexes of Cu and Zn at low levels (Zn 60 ppm, Cu 85 ppm for weanling pigs and Zn 40 ppm, Cu 60 ppm for broiler chicks) are not different from that of high levels of inorganic sources in maintaining growth performance and serum concentration. The fecal excretions for Cu and Zn were greatly reduced when organic sources were used.

• Journal of electromagnetic engineering and science
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• 제7권1호
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• pp.1-6
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• 2007

Recently, Mc(Multi-code) modulation/demodulation(modem) technique has been explored for high speed data transmission in wireless environment. The conventional Mc modem generates some side effects such as allocating Walsh codes, which motivates to propose a novel Mc modem method with sub-code. Our proposed system should expanded the size of sub-code to provide high-rate data transmission, which also affect adversely to the performance of the system with high PAPR(Peak to Average Power Ratio). Thus, in this paper, we propose a novel pre-coded Multi-Dimensional HCOC(High Capacity Orthogonal Code) Mc modem technique to reduce the high PAPR, which enables the performance improvement. This proposed system can be easily designed by concatenating HCOC Mc modem with the generic Mc modem. The pre-coding technique that is used in this paper is CAC(Constant Amplitude Coding), that helps the system maintain the constant transmission power and reduce the maximum transmission power.

• Computers and Concrete
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• 제16권1호
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• pp.1-16
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• 2015

The proposed techniques to strengthen concrete members such as steel plates, polymers or concrete have important deficiencies in adherence and durability. The use of UHPFC plates can overtake effectively these problems. In this paper, the possibility of using UHPFC to strengthen RC beams under torsion is investigated. Four specimens of concrete beams reinforced with longitudinal bars only were tested under pure torsion. One of the beams was considered as the baseline specimen, while the others were strengthened by ultra-high-performance fiber concrete (UHPFC) on two, three, and four sides. Finite element analysis was conducted in tandem with experimental work. Results showed that UHPFC enhances the strength, ductility, and toughness of concrete beams under torsional load, and that finite element analysis is in good agreement with the experimental data.

• Computers and Concrete
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• 제9권1호
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• pp.1-19
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• 2012

The size-effect study of various fracture parameters obtained from two parameter fracture model, effective crack model, double-K fracture model and double-G fracture model is presented in the paper. Fictitious crack model (FCM) for three-point bend test geometry for cracked concrete beam of laboratory size range 100-400 mm is developed and the different fracture parameters from size effect model, effective crack model, double-K fracture model and double-G fracture model are evaluated using the input data obtained from FCM. In addition, the fracture parameters of two parameter fracture model are obtained using the mathematical coefficients available in literature. From the study it is concluded that the fracture parameters obtained from various nonlinear fracture models including the double-K and double-G fracture models are influenced by the specimen size. These fracture parameters maintain some definite interrelationship depending upon the specimen size and relative size of initial notch length.

• Computers and Concrete
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• 제18권1호
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• pp.113-137
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• 2016

Extensive experimental studies on remarkable mechanical properties Polypropylene Fibre Reinforced Self-compacting Concrete (PFRSCC) have been executed, including different fibre volume fractions of Polypropylene fibers (0.25%, 0.5%, 0.75%, and 1%) and different water to cement ratios (0.21, 0.34, 0.38, and 0.41). The experimental program was carried out by using two hundred and sixteen specimens to obtain the impact resistance and mechanical properties of PFRSCC materials, considering compressive strength, splitting tensile strength, and flexural strength. Statistical and analytical studies have been mainly focused on experimental data to correlate of mechanical properties of PFRSCC materials. Statistical results revealed that compressive, splitting tensile, and flexural strengths as well as impact resistance follow the normal distribution. Moreover, to correlate mechanical properties based on acquired test results, linear and nonlinear equations were developed among mechanical properties and impact resistance of PFRSCC materials.

• Computers and Concrete
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• 제5권1호
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• pp.21-36
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• 2008

Steel fiber reinforced concrete is increasingly used day by day in various structural applications. An extensive experimentation was carried out with w/cm ratio ranging from 0.25 to 0.40, and fiber content ranging from zero to1.5 percent by volume with an aspect ratio of 80 and silica fume replacement at 5%, 10% and 15%. The influence of steel fiber content in terms of fiber reinforcing index on the compressive strength of high-performance fiber reinforced concrete (HPFRC) with strength ranging from 45 85 MPa is presented. Based on the test results, equations are proposed using statistical methods to predict 28-day strength of HPFRC effecting the fiber addition in terms of fiber reinforcing index. A strength model proposed by modifying the mix design procedure, can utilize the optimum water content and efficiency factor of pozzolan. To examine the validity of the proposed strength model, the experimental results were compared with the values predicted by the model and the absolute variation obtained was within 5 percent.

• Computers and Concrete
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• 제5권1호
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• pp.75-88
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• 2008

This paper presents a mathematical model for strength and porosity of mortars made with ternary blends of ordinary Portland cement (OPC), ground rice husk ash (RHA) and classified fly ash (FA). The mortar mixtures were made with Portland cement Type I containing 0-40% FA and RHA. FA and RHA with 1-3% by weight retained on a sieve No. 325 were used. Compressive strength and porosity of the blended cement mortar at the age of 7, 28 and 90 days were determined. The use of ternary blended cements of RHA and FA produced mixes with good strength and low porosity of mortar. A mathematical analysis and two-parameter polynomial model were presented for the strength and porosity estimation with FA and RHA contents as parameters. The computer graphics of strength and porosity of the ternary blend were also constructed to aid the understanding and the proportioning of the blended system.