• Journal of Plant Biotechnology
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• 제31권2호
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• pp.95-101
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• 2004

L. longiflorum cv. Gelria를 자방친으로 하고 Oriental group를 화분친으로 한 98조합 2,380개체에 대한 종간교배 결과 삭과 형성수는 7조합에서 11개체를 얻었다. 그중 미숙배를 형성한 삭과수는 L. 'Vettorea'를 화분친으로 한 1조합에서만 1개체를 얻어 그 비율은 미미했으나 1개의 삭과에서 11개의 미숙배를 획득했다. 같은 나팔백합인 L. longiflourm cv. Lorina를 자방친으로 하고 Oriental group를 화분친으로 한 74조합 1,265개체의 교배결과 삭과 형성 수는 8조합에서 9개체를 얻었다. 배형성 삭과율은 1.3%를 나타냈으며 전체 24개의 배를 얻었으며 삭과당 평균배수는 3.0개였다. L. ${\times}$formolongi cv.'Raizan'을 자방친으로 하고 Oriental group를 화분친으로 한 85조합 1,065개체 교배결과 삭과 형성수는 36조합에서 62개체를 얻었다. 그중 미숙배를 형성한 삭과수는 12조합에서 17개체를 얻었고 여기에서 얻은 전체 배수는 68개로 삭과당 평균배수는 4.0개였다. 같은 Longiflorum group인 L. ${\times}$formolongi과 L. ${\times}$formolongi 자방친으로 한 종간교배 결과를 비교해 보면 배형성 삭과율은 L. ${\times}$formolongi cv. Raizan (3.1%) 이 ㅣ. longiflorum cv. Gelria (0.1%)와 L. longiflorum cv. Lorina (1.3%) 보다 높은 편이었고, 삭과당 평균배수는 L. longiflorum cv. Gelria(11.0개)가 L. longiflorum cv. Lorina (3.0개), L. ${\times}$formolongi cv. Raizan (4.0개) 보다 높은 편이었다. 한편 Longiflorum group인 L. longiflourm cv. Gelria 및 L. longiflorum cv. Lorina와 L. ${\times}$formolongi cv. Raizan를 자방친으로 하고 화분친을 Martagon group인 1. distichum, L. hansonii, L. tsingtauense, L. martagon, Trumpet group인 L. 'Black Beauty', 1. henf, L. henry, regale 등으로 화주절단수분을 한 결과 (L. ${\times}$formolongi${\times}$L. henryi)의 조합에서만이 15개의 교배개체 중에서 2개의 성숙 삭과를 얻었을 뿐 배를 형성한 삭과는 얻지 못했다. 이러한 교잡장애를 극복하기 위해서는 본 시험에서 시행한 화주절단수분법 이외에도 지금까지 보고된 여러 가지 방법들을 적극 적용하여 금후 검토해볼 필요가 있을 것이다.

• 한국미생물·생명공학회지
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• 제43권2호
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• pp.126-133
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• 2015

본 연구에서는 정치 배양법으로 고농도의 초산을 생산할 수 있고 에탄올 내성이 우수한 균주를 확보하고자 농가형 발효식초에서 초산균을 분리 및 선발하였고, 이들 초산균의 형태적 특징을 조사한 바, 분리균 CV1은 그람 음성으로 운동성이 없는 간균으로 나타났다. 분리균의 chemotaxonomy를 분석한 결과, meso-DAP이며, 대표 퀴논은 Q₁₀이고, G+C mol 함량은 61.0 mol %로 나타났으며 16S rDNA 유전자의 염기서열을 분석한 결과, Gluconacetobacter saccharivorans로 동정되어 Glu. saccharivorans CV1로 명명하였다. CV1 초산균의 최적 성장조건은 30℃, pH 3.0 이상으로 판단되었고 에탄올 농도에 따른 초산 생성능은 10% 에탄올 농도에서 9.3%, 9% 에탄올 농도에서는 8.4% 적정산도를 나타내어 고농도 에탄올 조건에서도 높은 산 생성능을 나타내는 우수한 균주로 판단되었다.

• Korean Journal of Acupuncture
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• 제26권3호
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• pp.43-54
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• 2009

Objectives : To investigate the anti-inflammatory effects of Prunella vulgaris pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods : Sprague-Dawley rats were divided into 5 groups; normal control (n=8), LPS control (n=8), LPS+Prunella vulgaris pharmacopuncture at CV4 (CV4, n=8), LPS+Prunella vulgaris pharmacopuncture at ST36 (ST36, n=8), and LPS+Prunella vulgaris pharmacopuncture at CV12 (CV12, n=8). Pharmacopuncture was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5mg/kg). Proinflammatory cytokines including interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-10 (IL-10), thiobarbituric acid reactive substance (TBARS) from blood and liver tissue were compared before and 5 hrs after inflammation induction. Results : In CV4 and CV12 groups, plasma IL-$1{\beta}$, IL-6 and TNF-$\alpha$ levels increased by LPS injection, significantly decreased 5 hrs after injection (p<0.05). For CV12 group, plasma IL-10 concentration significantly increased (p<0.05). Liver IL-$1{\beta}$ and IL-6 levles significantly decreased in CV4 and CV12 groups (P<0.05), while normal and LPS control groups were not significantly different in TNF-$\alpha$ and IL-10 levels. Plasma TBARS concentration was significantly decreased in CV12 group, while there was no significant difference among LPS control and pharmacopuncture groups for liver TBARS concentration. Conclusions : Based on the present findings, Prunella vulgaris pharmacopuncture at CV12 may have a potentially preventive anti-inflammatory effect in an LPS-induced inflammatory rat model.

• 한국동물위생학회지
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• 제39권1호
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• pp.59-63
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• 2016

Duck circovirus (DuCV) has been recognized as a contagious immunosuppressive virus affecting many duck species worldwide. To determine the prevalence of DuCV infection in ducks, we investigated 104 samples collected from 50 duck farms in Jeonbuk province with the polymerase chain reaction (PCR). Among collected samples, 40 (38.5%) were positive for DuCV. The prevalence of DuCV PCR-positive samples increased with age. Genomic sequences of 15 DuCV strains were determined and compared with previously available DuCV sequences in public databases. Phylogenetic analysis revealed that all strains were clustered into DuCV-1 group.

• Korean Journal of Acupuncture
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• 제40권3호
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• pp.99-108
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• 2023

Objectives : The aim of this study was to assess the quantitative characteristics of pressure pain threshold (PPT) and pressure depth (PD) at the abdominal conception vessel (CV) acupoints according to subjective digestive status and digestive discomfort levels, accomplished by comparing a large group of healthy men and women, using the modified digital algometer. Methods : A total of 1,504 healthy adults aged 19 years or older participated in this study. A questionnaire was administered to evaluate participants' digestive status and discomfort. PPT (kgf/cm²/s) and PD (mm) measurements were obtained at CV14, CV12, and CV4 acupoints using a modified digital algometer. General characteristics were analyzed using the chi-square test, and differences in PPT and PD were assessed using two-sample t-tests and ANCOVA. Results : Significant difference in PPT was found based on digestive status at CV14 and CV12 for both sexes, and in PD at CV14 for women. Women exhibited significant difference in PPT based on digestive discomfort at CV14, CV12, and CV4, while men showed significant difference at CV14 and CV12. Significant difference in PD was observed at CV14 and CV4 among women. Even after adjusting for age and body mass index, significant difference persisted in PPT based on digestive discomfort at CV14 and CV12 for both sexes. PD exhibited significant differences at CV14, CV12, and CV4 among women. Conclusions : Using the modified digital algometer, this study showed the significant difference of pressure pain threshold at the CV14 and CV12 acupoints for subjective digestive discomfort levels in healthy men and women.

• 한국식품과학회지
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• 제29권4호
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• pp.817-822
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• 1997

구름버섯 자실체에서 분리된 항응고성 다당류의 혈액응고 저해기작을 검토하였다. 항응고성 다당(CV-40-Va-1)은 vWF의 활성을 감소시킴으로써 혈소판응집을 억제시키는 것으로 나타났으며, 혈액응고인자중 thrombin뿐만이 아니라 내인성경로의 factor VII, IX, XI, XII의 활성 또한 억제 하였다. 그러나 CV-40-Va-1의 항응고 활성은 thromin에 직접 작용하는 것이 아니라 antithrombin III 의존적 활성을 보였다. Sulfation에 의하여 CV-40-Va-1의 항응고활성의 증가와 다당의 desulfation시 상반된 결과에서 CV-40-Va-1의 황산기가 항응고활성의 중요한 인자임을 알 수 있었다. CV-40-Va-1을 TFA로 부분가수분해하여 얻은 획분들(Fr.I, Fr.II)에서는 항응고 활성이 감소하였으나 분자량 1,000정도로 추정되는 획분(Fr.II)은 오히려 혈소판응집을 강력하게 억제하였다.

• The Plant Pathology Journal
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• 제34권3호
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• pp.199-207
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• 2018

Tomato chlorosis virus (ToCV) is a whitefly-transmitted and phloem-limited crinivirus. In 2013, severe interveinal chlorosis and bronzing on tomato leaves, known symptoms of ToCV infection, were observed in greenhouses in Korea. To identify ToCV infection in symptomatic tomato plants, RT-PCR with ToCV-specific primers was performed on leaf samples collected from 11 tomato cultivating areas where ToCV-like symptoms were observed in 2013 and 2014. About half of samples (45.18%) were confirmed as ToCV-infected, and the complete genome of 10 different isolates were characterized. This is the first report of ToCV occurring in Korea. The phylogenetic relationship and genetic variation among ToCV isolates from Korea and other countries were also analysed. When RNA1 and RNA2 are analysed separately, ToCV isolates were clustered into three groups in phylogenetic trees, and ToCV Korean isolates were confirmed to belong to two groups, which were geographically separated. These results suggested that Korean ToCV isolates originated from two independent origins. However, the RNA1 and RNA2 sequences of the Yeonggwang isolate were confirmed to belong to different groups, which indicated that ToCV RNA1 and RNA2 originated from two different origins and were reassorted in Yeonggwang, which is the intermediate point of two geographically separated groups.

• 대한한방체열의학회지
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• 제5권1호
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• pp.46-58
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• 2006

Purpose: We intended to observe the relations between Chiljehyangbuhwan and abdominal & palmar temperature according to primary dysmenorrhea severity. Methods: We selected the 100 primary dysmenorrhea patients by the screening tests (first screening test-inquiry, second screening test-clinical test. additionally WHR (Waist-to-Hip ratio) by Inbody 2.0). By the fixed blocked randomization and double blind method, Chiljehyangbuhwan or placebo was administered for 1 menstration period. Finally, 69 patients remainded. Before and after administration, we measured 4 points abdominal temperature (Chon-jung (CV17), Chung-wan(CV12), Kwan-won(CV4). Chung-guk(CV3)) by DITI(DOREX Inc., USA). And then we checked the difference of temperature $({\Delta}T)$ between CV17 and CV12/CV17 and CV4/CV17 and CV3/CV12 and CV4/CV12 and CV3. Also, we measured 2 points (palmar region, upper front of forearm) for the difference of palmar temperature $({\Delta}T)$. Then, we checked palmar temperature minus upper front of forearm temperature and took an average of right and left ${\Delta}T.$ After that. we compared ${\Delta}T$ with primary dysmenorrhea severity evaluated by VRS (verbal rating scale) and MVRS (multidimensional verbal rating scale). In dysmenorrhea severity. we standardized scale score and 3-group-severity by score (mild, moderate. severe). Besides, we compared palmar ${\Delta}T$ with abdominal ${\Delta}T$. For statistics, we used ANOVA and Spearman's rho correlations. SPSS 13.0 for windows. Results: In case of MVRS, though Chiljehyangbuhwan was correlated to abdominal ${\Delta}T$(CV12 and CV3/CV12 and CV4). it was not correlated to palmar ${\Delta}T$. In case of VRS, though Chiljehyangbuhwan was not correlated to abdominal ${\Delta}T$. it was correlated to palmar ${\Delta}T$. However. palmar ${\Delta}T$ was not correlated to abdominal ${\Delta}T$. Statistically they showed significant result (p<0.05). Conclusion: The primary dysmenorrhea patients showed that severity by MVRS was connected with abdominal ${\Delta}T$ (CV12 and CV3/CV12 and CV4) and severity by VRS was connected with palmar ${\Delta}T$ after Chiljehyangbuhwan administration. So we can consider Chiljehyangbuhwan partially effects the abdominal & palmar temperature according primary dysmenorrhea severity. However, palmar temperature was not correlated to abdominal temperature. Therefore, we need further study.

• Korean Journal of Acupuncture
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• 제21권1호
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• pp.175-199
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• 2004

Objectives : To study acupuncture and moxibution treatments for stomatopathy( aphthae, oral ulceration, mycolic stomatitis, halitosis, thirst, bitter) the ancient and the present literatures were reviewed. Methods : We've got compared and analyzed 55 kinds of literatures. Results and Conclusions : 1. The acupuncture meridians used frequently for stomatopathy were $Su-yangmy\bar{o}ng-Taejang-ky\bar{o}ng(LI),\;Chok-yangmy\bar{o}ng-Wi-Ky\bar{o}ng(S),\;Immaek-Ky\bar{o}ng(CV)$. 2. The acupoints used frequently for aphthae were $Sungjang(CV_{24}),\;Yomchon(CV_{23}),\;Hapkok(LI_4),\;Nogung(P_8),\;Chok-samni(S_{36}),\;Hyopko(S_6),\;Sugu(GV_{26})$. 3. The acupoints used frequently for oral ulceration were $Hapkok(LI_4),\;Nogung(P_8),\;Chok-samni(S_{36}),\;Kokchi(LI_{11}),\;Sotaek(SI_1),\;Pisu(B_{20}),\;Wisu(B_{21}),\;Samgan(LI_3),\;Yomchon(CV_{23}),\;Chichang(S_4)$. 4. The acupoints used frequently for mycolic stomatitis were $Hapkok(L_4),\;Chichang(S_4),\;Hyopko\;(LI_4),\;Sungjang(CV_{24}),\;Samumgyo(SP_6)$. 5. The acupoints used frequently for halitosis were $Naejong(S_{44}),\;Chok-samni(S_{36}),\;Chung-wan\;(CV_{12}),\;Sang-wan(CV_{13}),\;Hawan(CV_{10}),\;Kongson(SP_4),\;Wisu(B_{21}),\;Nogung(P_8),\;Sugu(GV_{26}),\;Sungjang(CV_{24})$. 6. The acupoints used frequently for thirst were $Sosang(L_{11}),\;Sangyang(LI_1),\;Sotaek(SI_1),\;Kwanch'ung(TE_1),\;Ch'\bar{o}kt'aek(L_5),\;T'ae-gye(K_3),\;Kokt'aek(P_3),\;Sugu(GV_{26}),\;Samgan(LI_3),\;Igan(LI_2),\;T'aech'ung(Liv_3),\;Sojangsu(B_{27})$. 7. The acupoints used frequently for bitter were $Yangn\bar{u}ngch'\bar{o}n(G_{34}),\;Hy\bar{o}njong(G_{39}),\;Kwanch'ung(TE_1),\;Tamsu(B_{19}),\;Chokkyu\bar{u}m(G_{44}),\;Y\bar{o}n-gok(K_2),\;Shinmun(H_7),\;Chok-Samni(S_{36})$.

• 한국발생생물학회지:발생과생식
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• 제20권1호
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• pp.41-49
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• 2016

Moxibustion, which has a more than 4,000-year long history of use in East Asia, has been widely used for gynecological diseases. To investigate the effects of perineum (CV1) moxibustion on gynecological function, infertile women of childbearing age applied CV1 moxibustion for 30 days on a fixed schedule. The subjects had on average $2.4{\pm}1.1$ cases of infertility-related disabilities. After CV1 moxibustion treatment, on average, the subjects showed improvements in $1.5{\pm}1.0$ cases of their gynecological problems. In addition, 14.7% of the subjects got pregnant within $29{\pm}1.4$ days after the treatment. This result shows that application of moxibustion on the CV1 and uterus is an effective treatment for gynecological diseases and that it can improve the function of gynecologic organs.