• 제목/요약/키워드: CULTURE

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토끼 성장판 연골세포 배양과 자가 이식편에서의 형태학적인 변화 (Chondrocyte Culture from Epiphyseal Plate and its Morphological Changes in Autologous Implants of Rabbit)

  • 양영철;정해일;최장석
    • 생명과학회지
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    • 제10권4호
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    • pp.408-421
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    • 2000
  • We tried to establish the culture method of the chondrocyte isolated from the epiphyseal cartilage and to investigate morphological changes of chondrocyte cultured with enzyme-digested costal cartilage, the perichondrium and experimentally damaged meniscus of rabbit. De novo chondrocyte pellets were prepared from epiphyseal plates by culturing isolated epiphyseal chondrocytes from 4 week. old rabbits. We morphologically assessed the cartilage formation of the chondrocyte culture with enzyme-digested costal carilage, the perichondrial culture, the cultured chondrocytes transplants into experimentally damaged meniscus of rabbits, the perichondrial culture, the cultured chondrocytes transplants into experimentally damaged meniscus of rabbit. In the 24 days, the epiphyseal chondrocytes maintained the typical phenotypes of the partial nodular cell formation. The 30 days cryopreserved chondrocytes showed abnormal and irregular shape. In the type II collagen added culture, the chondrocytes showed expanded rough endoplasmic reticulum and small and large round-like vesicles of processes. In the type IV collagen added culture, the chondrocytes showed large perinuclear vaculoes and abundant well-developed rough endoplasmic reticulum of processes. In the culture with enzyme- digested costal cartilage and the perichondrial culture, the chondrocytes showed a few swelling rough endoplasmic reticulum and vacuoles. The cultured epiphyseal chondrocytes maintained typical phenotype and the chondrocytes were grown faster and maintained more typical phenotype in the type II and IV collagen added culture. The transformed chondrocytes secreted abundant extracellular matrix in the type II collagen added culture, and showed processes in the type IV collagen added culture. The perichondrial chondrocytes were grown faster and their implants were able to transplant. The cultured chondrocytes transplanted into experimentally damaged meniscus were adapted between the meniscus tissues. And the immunocyto-chemical reaction of the type II collagen of the chondrocytes were found to be maintained. The chondrocytes cultured cartilage. The chondrocytes secreted abundantly. The cultured chondrocytes transplanted into experimentally damaged meniscus changed immature cells into enlarged mature cells with extracellular secretion.

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느타리버섯 멀칭재배 방법과 수확도구 (Mulching Cultivation Method and Harvesting Implement of Pleurotus ostreatus)

    • 한국자원식물학회지
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    • 제12권3호
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    • pp.179-185
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    • 1999
  • 본 연구에서 얻어진 느타리버섯 멀칭재배의 결과를 요약하면 다음과 같다. 1) 상자재배 방법은 관행시 1695g/상자, 흑색비닐 멀칭시 24.2%가 증수된 2237g/상자, 백색비닐 멀칭시 16.4%가 증수된 2028g/상자이었다. 2) 균상재배 방법은 관행시 48.2kg/3.3m$^2$ 흑색비닐 멀칭시 57.5kg/3.3m$^2$, 백색비닐 멀칭시 52.1kg/3.3m$^2$로 각각 16.2%와 7.5%의 증수 효과가 있었다. 3) 자루재배시 느타리버섯의 발이구멍 직경은 5~30mm, 간격은 10cm일 경우 양호하였다. 4) 느타리 버섯 멀칭재배는 세균성갈변병, 푸른곰팡이, 버섯파리의 예방효과가 있었다. 5) 느타리버섯 흑색 비닐멀칭 자루재배는 균상, 상자, 봉지재배 보다 수량이 많고 품질이 좋았다. 6) 멀칭 수확도구는 45~120cm $\times$ 45~200cm 길이가 적당하였다.

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전통문화축제의 내실화방안 연구 (Developing Proposals for Korean Traditional Culture Festivals)

  • 정달영;박기종
    • 한국연극학
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    • 제48호
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    • pp.549-569
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    • 2012
  • Since 1995, the Local autonomy era has opened, local festivals have been increasing throughout the country. Traditional culture festivals are also increasing. Performing traditional culture festivals is effective for maintenance and transmission of traditional culture as well as creation and nurturing of local culture. They are also effective promoting local economy. Depending on realization level of character, place and costume based on historic research, traditional culture festivals are classified as the "Representation event" or the "Reenactment event". But there are two problems. The first is unexacting classification method for festival types made by Central government yearly. The second is unable to meet one of two goals which are protecting traditional culture and stimulating local economy throughout increasing tourists. The purpose of this study is to suggest improvement of classification method for festival types, and to offer two suggestions for ensure successful local festivals. First of all, I suggest advanced classification method for festival types. For more sophisticated collection process of national festival status, local government department should ensure purpose of festivals, and central government department should add 3 steps to existing process. For example professional committee for judgement of festival types should be founded for consulting of local and central government department. The second suggestion is reinforcement of historic research for the Representation of traditional culture event. The representation of traditional culture should focus on protection of tradition, and could be perfect by continuous historic research. The last suggestion is cooperation with local governments each other for the Reenactment of traditional culture event. The Reenactment of traditional culture should focus on promoting local economy by increasing tourists. So local governments who have similar traditional events should cooperate to get preventing loss of resource and overcome weakness of promoting.

Graded approach to determine the frequency and difficulty of safety culture attributes: The F-D matrix

  • Ahn, Jeeyea;Min, Byung Joo;Lee, Seung Jun
    • Nuclear Engineering and Technology
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    • 제54권6호
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    • pp.2067-2076
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    • 2022
  • The importance of safety culture has been emphasized to achieve a high level of safety. In this light, a systematic method to more properly deal with safety culture is necessary. Here, a decision-making tool that can apply a graded approach to the analysis of safety culture is proposed, called the F-D matrix, which determines the frequency and the difficulty of safety culture attributes recently defined by the IAEA. A hierarchical model of difficulty contributors was developed as a scoring standard, and its elements were weighted via expert evaluation using the analytic hierarchy process. The frequency of the attributes was derived by analyzing reported events from nuclear power plants in the Republic of Korea. Period-by-period comparisons with the F-D matrix can show trends in the change of the maturity level of an organization's safety culture and help to evaluate the effectiveness of previously implemented measures. In the evaluating the difficulty of the attributes in the recently developed harmonized safety culture model, the difficulties of Trending, Benchmarking, Resilience, and Documentation and Procedures were found to be relatively high, while the difficulties of Conflicts are Resolved, Ownership, Collaboration, and Respect is Evident were found to be relatively low. A case study was conducted with an analysis period of 10 years to attempt to reflect the many changes in safety culture that have been made following the Fukushima accident in March 2011. As a result of comparing two periods following the Fukushima accident, the overall frequency decreased by about 40%, providing evidence for the effects of the various improvements and measures taken following the increased emphasis on safety culture. The proposed F-D matrix provides a new analytical perspective and enables an in-depth analysis of safety culture.

미국내 발간된 한국음식관련 요리책에 나타난 한국음식과 음식문화 - 1930년대부터 1970년대 초까지 - (Korean Food Culture of Cookbooks Related to Korean Food Issued in the U.S. - From the 1930s to the Early 1970s -)

  • ;정희선
    • 한국식생활문화학회지
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    • 제37권4호
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    • pp.285-299
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    • 2022
  • This study reviewed Korean food recipes and food culture included in English cookbooks issued in the United States from the 1930s to the early 1970s. In the 1930s cookbook, many types of soup were introduced to Korean food under the influence of the Tangban culture in the Joseon dynasty and a brief description of 'Sinseollo' culture. The 1940s cookbooks, introduced Korean table settings, food culture, and cooking methods depending on the use of chopsticks. In the 1950s cookbooks, Korean foods were selected to suit a Western table setting, and detailed explanations were included along with 'cheopsu' means the number of dishes served in Korean food. More diverse Korean food was introduced within its culture and origins in the 1960s cookbooks. The 1970s cookbooks explained, the characteristics of Korean culinary specialties that differentiated from oriental food. This study of Korean food and culture from Cookbooks issued in the United States from the 1930s to 1970s, where Eastern and Western multiculturalism coexist, can be used as baseline data to understand the identity of modern Korean food culture and the direction of the globalization of Korean food.

초등학생의 식생활 실태 및 전통 식생활교육에 대한 인식 (Meal practice and Perceptions of Traditional Food Culture Education in Elementary School Students)

  • 윤선주;김희섭
    • 한국식생활문화학회지
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    • 제25권5호
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    • pp.558-567
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    • 2010
  • Changes in social, economical, and cultural environments affect the meal practices of children. The transmission of traditional Korean food culture is very important because it presents not only a well-balanced diet but also contributes to shaping identity. The purpose of this study was to investigate elementary school students' present meal practices and views, as well as demands on traditional food culture education to reflect future educational plans. Half of the students ate breakfast everyday and 72% ate a traditional Korean style breakfast. About 38% of the students participated 2-4 times per week in meal preparation and 34% participated in clean-up after the meal once a day. Although 6th graders had greater skills in basic cooking, they tended to be more passive upon applying their skills in daily meal practice. For traditional food culture education, 89% of the experienced and 86.2% of the inexperienced groups agreed on the necessity of traditional food culture education. Students attained traditional food culture knowledge through Silgwa, practical coursework within the curriculum, and by teachers leading classes. They were also educated by parents, mass media, and books outside of school. The preferred methods of class teaching were lecture and experiential learning. The preferred subjects to learn were 'cooking classes based on taste development', 'learning food ingredients through vegetable growing', 'traditional Korean food manners', and 'traditional Korean food culture and seasonal foods' as well as nutritional education. Fifth graders had more positive attitudes towards meal practices and traditional food culture education. Traditional Korean food culture and nutrition education should be integrated and developed into regular subject curricula to improve children's meal practice and inheritance of traditional food culture.

조직문화 유형이 직무만족과 이직의사에 미치는 영향 - 국립병원 조직구성원을 대상으로 - (The Effect of Organizational Culture Types on Job Satisfaction and Intension of Turnover Perceived by National Hospital Employees)

  • 박재산
    • 한국병원경영학회지
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    • 제10권1호
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    • pp.1-24
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    • 2005
  • Organizational culture has been very important in the field of organizational behavior research for the past decade. Although there has been a growing interest in the organizational culture and organizational performance, there was few research in healthcare organizations. The objective of this study is to identify the relationship of organizational culture based on the Quinn's competing values approach, with job satisfaction and intention of turnover perceived by national hospital employees. The study setting was the national hospital. Data were collected by self-administered questionnaires. The study sample consisted of 555 hospital employees. Structural Equation Modeling(SEM) analysis was conducted to find the causal relationship of organizational culture, job satisfaction and intention of turnover.The major results of this study are as follows: the consensual culture, rational culture, and developmental culture in national hospital showed a strong relationship with job satisfaction and intention of turnover in contrast to other previous studies. This finding showed that task oriented and human related climate is more effective on job satisfaction than hierarchical culture in national hospital settings.

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대학병원 종사자의 조직구조, 조직문화에 따른 조직갈등의 관계에 대한연구 (The Relation of Organization Structure, Organization Culture and Organization Conflict of University Hospital Staff Members)

  • 이창호;조덕영;김윤진
    • 보건의료산업학회지
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    • 제9권1호
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    • pp.95-107
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    • 2015
  • The purpose of this study is to analyze the characteristics of hospital organization structure, organization culture, organization conflict and the interrelation of influential factor in organization conflict. The data for this study were collected through a self-administered survey with a structured delivered to 755 subjects including several nursing staff members, administration staff members and medical technicians at six hospitals. The structural equation model was used to examine the relation of organization structure, organization culture and organization conflict. The results of the analysis in this study were as follows. First, organization structure had a positive influence on organization conflict, especially in terms of complexity and centralization. But, formalization had a negative influence on organization conflict. Second, organization culture had a positive influence on organization conflict, especially in terms of group culture, developmental culture, and hierarchical culture. But, group culture had a negative influence on organization conflict. Third, organization structure had positive influences, especially on organization culture complexity, centralization, and formalization. But, centralization had negative influences too.

간호 조직문화 7S 요인과 조직 유효성의 관계 (The Relationship Between 7S Factors of the Nursing Organizational Culture and Organizational Effectiveness)

  • 하나선;박효미;최정
    • 간호행정학회지
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    • 제10권2호
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    • pp.255-264
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    • 2004
  • Purpose: The Purpose of this study was to identify the relationship between 7S factors of the nursing organizational culture and organizational effectiveness. Method: The data were gathered from the self-reported questionnaires of 717 nurses who work for eight different general hospitals located around Seoul and Kyounggi province. The period of data collection was from November 12 to December 7, 2002. For data analysis, descriptive statistics, clustering analysis, and t-test with SPSS Program were used. Result: The nurses who highly perceived 7S factors of nursing organizational culture showed higher job satisfaction and organizational commitment in comparison with the nurses who lowly perceived 7S factors of nursing organizational culture. And the nurses who highly perceived 7S factors of nursing organizational culture showed higher organizational citizenship behavior in comparison with the nurses who lowly perceived 7S factors of nursing organizational culture. Among subdimension of organizational citizenship behavior, altruism and civic virtue were significant. Conclusion: From the above results, the high group with 7S factors of nursing organizational culture has strong culture, therefore nursing organization with strong culture is very implicative to enhance the organizational effectiveness.

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약령 마우스에서 분리한 난소의 기관배양에 관한 연구 (Organ Culture of Ovary Isolated from Juvenile Mice)

  • 이현주;김지철;김기동;이상호;송해범
    • 한국수정란이식학회지
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    • 제17권3호
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    • pp.195-201
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    • 2002
  • 본 실험은 원시난포의 체외배양 체계를 확립할 수 있는 가능성을 검토하기 위해 0, 5, 10, 15 및 20일령 마우스에서 분리한 난소를 20, 15, 10, 5 및 0일동안 organ culture 하여 난소의 성장률, 난자의 회수율과 성장률 및 난자의 핵성숙 단계를 조사하여, 비교 ·검토하였다. 그 결과를 요약하면 다음과 같다. 1. Organ culture 전과 후의 각 일령에 따른 난소의 면적 차이는 0일령 35.9%, 5일령 8.7%, 10 일령 1.2% 및 15일령 14.4%로 15일령을 제외 하고는 배양일령이 증가할수록 면적 차이는 감소하였다. 2. Organ culture 후 난자의 회수율과 난자의 직경은 배양일령이 증가할수록 증대되었다. 3. GV기 이상의 핵성숙은 organ culture 후 배양 일령이 증가할수록 진행되는 단계에 있는 것으로 나타났다.