• Title/Summary/Keyword: CL response

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Cobalt Chloride-Induced Downregulation of Puromycin-Sensitive Aminopeptidase Suppresses the Migration and Invasion of PC-3 Cells

  • Lee, Suk-Hee;Kim, Hwan-Gyu
    • Journal of Microbiology and Biotechnology
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    • v.19 no.5
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    • pp.530-536
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    • 2009
  • Cobalt chloride ($CoCl_2$) treatment of cells in vitro has been shown to induce cellular changes that are similar to those seen following hypoxia. To identify genes that are differentially expressed in response to treatment with $CoCl_2$, we compared the mRNA expression profiles of PC-3 cells that were treated with $CoCl_2$ with those of untreated PC-3 cells, using specific arbitrary primers and two anchored oligo(dT) primers provided in the ACP-based GeneFishing kits. The results of this study demonstrated that the puromycin-sensitive aminopeptidase (PSA) gene was down regulated in PC-3 cells that were treated with $CoCl_2$. This downregulation of PSA expression, in turn, suppressed the proliferation, migration, and invasion of PC-3 cells, as well as the secretion and expression of matrix metalloproteinase-9 (MMP-9).

Electrical and Optical Properties of Electrochromic Window with Both Lithium and Proton Conducting Polymer Electrolytic Media (리튬 및 프로톤 전도성 고분자전해질을 사용하여 제작한 Electrochromic 창의 전기 및 광학적 특성)

  • 박성용;이철환;김형선;조원일;조병원;윤경석;안춘호;우경근
    • Journal of the Korean institute of surface engineering
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    • v.28 no.1
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    • pp.46-54
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    • 1995
  • An electrochromic(EC) cell was constructed using $WO_3$ as a electrochromic material and NiO as a counter electrode, deposited onto ITO-coated glass by the implementation of electron beam evaporation. The electrolytic media were both lithium and proton conducting polymers such as poly-acrylonitrile(PAN)-$LiClO_4$, poly-ethylene oxide(PEO)-$LiClO_4$, poly-vinyl butyral(PVB)-LiCl and PVB-H$_3$$PO_4$. Potentiodynamic cycling of the cells using PAN-$LiClO_4$, or PVB-$H_3$$PO_4$ electrolyte yielded a transmission variation of more than 40% at the wavelength of 632.8 nm within less than 10 sec response time at room temperature. These results indicate that these electrolytes, transparent in gel type, are premising for the application in large area electrochromic windows.

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Study on the Sister Chromatid Exchange Inducibility in Chinese Hamster Don Cell by Metal Compounds in Work Enviroment

  • Seo, Kwang-Seok;Lee, Chong-Sam
    • Journal of Environmental Health Sciences
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    • v.22 no.1
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    • pp.91-98
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    • 1996
  • 산업장이나 생활환경에서 접하기 쉬운 수용성 염화물을 중심으로 19개 원소 24종의 금속화합물이 Chinese Hamster Don 세포에 있어서의 sister chromatid exchange(SCE) 출현빈도에 미치는 영향을 조사하였다. Chinese Hamster Don 세포에 대한 자매염색분체 교환출현빈도의 증가가 $CrO_3, K_2CrO_4, K_2Cr_2O_7, MnCl_2, K_2SeO_3, CH_3HgCl$ (p<0.01), $CoCl_2, Na_2HAsO_4, HgCl_2$ (p<0.05) 9종의 금속화합물에서 나타났으며, dose-response relationships이 현저한 금속화합물은 6가 크로화합물과 $K_2SeO_3$이었다.

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Reduction of AgCl to Ag by $Na_2CO_3$ ($Na_2CO_3$에 의한 AgCl의 Ag 환원)

  • 박경호;노범식;손정수
    • Resources Recycling
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    • v.5 no.1
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    • pp.29-33
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    • 1996
  • The cominnn plocesses lor rccoremg silver irom silvcr conlaincd waster are the lcachmg silver hy HNO;. the srlcctive precipilillion of sliver ion lo AgCl and thc rcduchon of Ag wrfh ;I proper reductant. In this sludy, thc reduction of AgCI lo Ag was invesllngated by using Na, CO, as a rcd\icta~lt. The variations wcic reaction time. ttmpcrarure thc amount of NalCO, . and the resulls %, ere analyzcd by using sialist~c:d tecl~niques such as the ]polynomial rcgressiun analysis and the response surh~ce method. More than Yh% Ag analyzed was rcduced 1rtm AgCI at 62UT. I hour ullder condillon of 2 stnlchio~nctric ratio of Na iCO, !AgCI.

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Immobilized Luminescent Cell - based Flow Through Monitoring of Environmental Pollutants

  • Britz, Margaret L.;Simonov, Nina;Chun, Uck-Han
    • Journal of Microbiology and Biotechnology
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    • v.7 no.4
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    • pp.250-257
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    • 1997
  • A new sensing system based on the immobilization of luminescent bacteria, photobacterium phosphoreum, was proposed for continuous real-time monitoring of pollutants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate were very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb$(NO_3)_2$), $NiCl_2$, $CdCl_2$, 50 ppm for $NaASO_2$, 0.1 ppm for $HgCl_2$, 0.5 ppm for pentachlorophenol and less than 5 ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of $-80^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) was optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol. A rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

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Proline Accumulation in Vigna angularis Seedlings Under Salt Stress

  • Lee, Hee-Kyung;Hong, Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.4 no.1
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    • pp.51-57
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    • 2000
  • Changes in the proline accumulation of ten-day-old seedlings of Vigna angularis in response to NaCl treatment were monitored. The proline content increased gradually both with an increase in the exposure time to salt stress and in a concentration-dependent manner. The increased proline accumulation was stronger in the shoots than in the roots. The salt stress by itself resulted in a significant inhibition of the chlorophyll content. Pre-treatment with proline before salinization lasting 48 h did not significantly affect the endogenous proline level in the roots, in contrast, a considerable increase of proline was observed in the shoots. The application of exogenous proline to the seedlings increased the endogenous proline content and improved the root and shoot growth under saline conditions. Detached leaves also exhibited an increased proline level in response to the applied NaCl, however, at a lower magnitude than in the intact seedlings. The proline alleviated the inhibitory effect of the NaCl in a concentration-dependent manner, thereby suggesting that salinity is a strong inducer of proline accumulation. In addition, abscisic acid eliminated the inhibitory effect of the salt salinity, thereby indicating a protective role on salinity stress and a regulatory role in proline synthesis. Accordingly, it would appear that proline may be involved in salt tolerance.

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Optimization of Liquid Desiccant Cooling Cycle (액체 제습식 냉방 사이클의 최적화)

  • Kim, Seon-Chang;Kim, Young-Lyoul;Lee, Sang-Jae;Jeon, Dong-Soon;Choi, Jaug-Hyeon;Kwon, Hyeok-Min;Lee, Chang-Jun
    • Proceedings of the SAREK Conference
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    • 2009.06a
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    • pp.673-678
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    • 2009
  • This paper presents the optimization process of liquid desiccant cooling cycle using LiCl aqueous solution as a working fluid. Operating conditions and design factors for heat exchangers were optimized by response surface method. As a result, we obtained the 7.297 kW of cooling capacity and 0.788 of COP at optimized condition. Effect of $dT_{hw}$ on system performances was also examined. As $dT_{hw}$ increases, the cooling capacity increases and COP decreases.

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Evaluation of Electrospun TiO2/PVP/LiCl Nanofiber Array for Humidity Sensing (전기방사를 이용한 TiO2/PVP/LiCl 나노섬유 습도 센서의 제작과 평가)

  • Ryu, Hyobong;Kim, Bumjoo;Kwon, Hyukjin Jean;Heo, Joonseong;Lim, Geunbae
    • Journal of Sensor Science and Technology
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    • v.23 no.1
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    • pp.42-45
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    • 2014
  • Recently, tremendous application utilizing electrospun nanofibers have been actively reported due to its several advantages, such as high surface to volume ratio, simple fabrication and high-throughput manufacturing. In this paper, we developed highly sensitive and consistent nanofiber humidity sensor by electrospinning. The humidity sensor was fabricated by rapid electrospinning (~2 sec) $TiO_2$/PVP/LiCl mixed solution on the micro-interdigitated electrode. In order to evaluate the humidity sensing performances, we measured current response using DC bias voltage under various relative humidity levels. The results show fast response / recovery time and marginal hysteresis as well as long-term stability. In addition, with the aid of micro-interdigitated electrode, we can reduce a total resistance of the sensor and increase the total reaction area of nanofibers across the electrodes resulting in high sensitivity and enhanced current level. Therefore, we expect that the electrospun nanofiber array for humidity sensor can be feasible and promising for diverse humidity sensing application.

Immobilization of Photobacterium Phosphoreum for Monitoring of Toxic Substances

  • Uck-Han Chun;Jun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.2 no.2
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    • pp.141-146
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    • 1997
  • A new sensing system based on the immobilization of luminescent batcteria, Photobacterium phosphoreum, was proposed for continuous real-time monitoring of polluants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate was very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb(NO3)2, NiCl2, CdCl2, 50 ppm for NaAsO2, 0.1ppm for HgCl2, 0.5ppm for pentachlorophenol and less than 5ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of -8$0^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) were optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol and a rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

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Optimization Design of Liquid Desiccant Cooling System (액체 제습식 냉방 시스템의 최적 설계)

  • Jeon, Dong-Soon;Lee, Sang-Jae;Kim, Seon-Chang;Kim, Young-Lyoul;Lee, Chang-Jun
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.22 no.7
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    • pp.419-428
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    • 2010
  • This paper presents the optimization process of liquid desiccant cooling system using LiCl aqueous solution as a working fluid. Operating conditions(mass flow rate, conditioner outlet concentration, difference concentration) and design factors for heat exchangers(difference temperature of the district heating water, leaving temperature difference of the conditioner, leaving temperature difference of the regenerator, air temperature difference of the conditioner, air temperature difference of the regenerator) were optimized by response surface method. As a result, we obtained the 7.297 kW of cooling capacity and 0.788 of COP at optimized condition. Effect of difference temperature of hot water on system performances was also examined. As difference temperature of the district heating water increases, the cooling capacity increases and COP decreases.