Biotechnology and Bioprocess Engineering:BBE
- Volume 2 Issue 2
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- Pages.141-146
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- 1997
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- 1226-8372(pISSN)
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- 1976-3816(eISSN)
Immobilization of Photobacterium Phosphoreum for Monitoring of Toxic Substances
- Uck-Han Chun (Department of Food Technology and Science, College of Industry, Kyung Hee University) ;
- Jun (Department of Food Technology and Science, College of Industry, Kyung Hee University Department of Food Technology and Science, College of Industry, Kyung Hee University Department of Food Technology and Science, College of Industry, Kyung Hee University)
- Published : 1997.12.01
Abstract
A new sensing system based on the immobilization of luminescent batcteria, Photobacterium phosphoreum, was proposed for continuous real-time monitoring of polluants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate was very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb(NO3)2, NiCl2, CdCl2, 50 ppm for NaAsO2, 0.1ppm for HgCl2, 0.5ppm for pentachlorophenol and less than 5ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of -8