• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.23-29
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• 2009

We have previously isolated a CCCH type zinc-finger protein gene, OsZF2 (Oryza sativa Zinc Finger 2), from the cold-treated rice cDNA library. To investigate the potential role of OsZF2, transgenic rice lines over-expressing OsZF2 under the control of CaMV 35S promoter have been developed through Agrobacterium-mediated transformation. Elevated level of OsZF2 transcripts was confirmed by RNA gel blot analysis in transgenic rice. Under the 100 mM NaCl condition, the transgenic rice showed significantly enhanced growth rate in terms of shoot length and fresh weight, implicating that OsZF2 is likely to be involved in salt response of rice. In the field condition, however, the transgenic rice showed a dwarf phenotype and flowering time was delayed. Genome expression profiling analysis of transgenic plants using the 20K NSF rice oligonucleotide array revealed many up-regulated genes related to stress responses and signaling pathways such as chaperone protein dnaJ 72, salt stress-induced protein, PR protein, disease resistance proteins RPM1 and Cf2/Cf5 disease resistance protein, carbohydrate/ sugar transporter, OsWAK kinase, brassinosteroid LRR receptor kinase, and jasmonate O-methyltransferase. These data suggest that the CCCH type zinc-finger protein OsZF2 is a upstream transcriptional factor regulating growth and stress responsiveness of rice.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.167-167
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• 2017

Our previous study for developing seeds of Iksan 526 (I.526), an inbred line of resveratrol-producing transgenic rice line, showed that, in 20 days after heading (DAH) seeds, resveratrol was almost saturated and accumulation of piceid was highest though the expression of Arachis hypogaea resveratrol synthase 3 (AhRS3, GenBank DQ124938) was highest in 31 DAH seeds. In this study, it was investigated how the overexpression of AhRS3 affects phenylpropanoid pathway genes. p-Coumaroyl-CoA is derived from phenylpropanoid pathway and used as a substrate of AhRS3 reaction for resveratrol production. In 6, 13, 20, 31 and 41 (45 for Dongjin) DAH seeds of I526 and Dongjin, a wild type of I.526, respectively, the expression pattern of phenylpropanoid pathway genes, including phenylalanine ammonia-lyase (PAL: LOC_Os02g41630.2, LOC_Os04g43760.1), cinnamate 4-hydroxylase (C4H: LOC_Os05g25640.1), 4-coumarate-CoA ligase (4CL: LOC_Os02g08100.1), cinnamoyl-CoA reductase (CCR: LOC_ Os09g25150.1, LOC_Os08g34280.1), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: LOC_Os04g42250.2, LOC_Os02g39850.1) and cinnamyl alcohol dehydrogenase (CAD: LOC_Os02g09490.1), was examined using real time (RT)-PCR. Compared to developing seeds of Dongjin, RT-PCR results showed that the expression pattern of phenylpropanoid pathway genes was modified in developing seeds of I.526. In most genes, except for CAD, of I.526 developing seeds, the gene expression was highest in 20 DAH corresponding to biosynthesis of resveratrol and piceid, i.e. the expression of phenylpropanoid pathway genes was gradually increased by 20 DAH and decreased as seeds develop. Especially, in Dongjin, the highest expression of PALs and 4CL was in 6 DAH and their expression was gradually decreased as seeds develop. These genes expression data also exhibited that, in developing seeds of I.526, phenylpropanoid pathway genes were slightly or significantly (in some genes) upregulated compared to Dongjin. Therefore, the overexpression of AhRS3 changed the expression pattern of phenylpropanoid pathway genes in I.526 developing seeds and this modification for gene expression is closely related to biosynthesis of resveratrol and piceid.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.105-105
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• 2017

Our previous study for developing seeds of Iksan 526 (I.526), an inbred line of resveratrol-producing transgenic rice line, showed that, in 20 days after heading (DAH) seeds, resveratrol was almost saturated and accumulation of piceid was highest though the expression of Arachis hypogaea resveratrol synthase 3 (AhRS3, GenBank DQ124938) was highest in 31 DAH seeds. In this study, it was investigated how the overexpression of AhRS3 affects phenylpropanoid pathway genes. p-Coumaroyl-CoA is derived from phenylpropanoid pathway and used as a substrate of AhRS3 reaction for resveratrol production. In 6, 13, 20, 31 and 41 (45 for Dongjin) DAH seeds of I526 and Dongjin, a wild type of I.526, respectively, the expression pattern of phenylpropanoid pathway genes, including phenylalanine ammonia-lyase (PAL: LOC_Os02g41630.2, LOC_Os04g43760.1), cinnamate 4-hydroxylase (C4H: LOC_Os05g25640.1), 4-coumarate-CoA ligase (4CL: LOC_Os02g08100.1), cinnamoyl-CoA reductase (CCR: LOC_Os09g25150.1, LOC_Os08g34280.1), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: LOC_Os04g42250.2, LOC_Os02g39850.1) and cinnamyl alcohol dehydrogenase (CAD: LOC_Os02g09490.1), was examined using real time (RT)-PCR. Compared to developing seeds of Dongjin, RT-PCR results showed that the expression pattern of phenylpropanoid pathway genes was modified in developing seeds of I.526. In most genes, except for CAD, of I.526 developing seeds, the gene expression was highest in 20 DAH corresponding to biosynthesis of resveratrol and piceid, i.e. the expression of phenylpropanoid pathway genes was gradually increased by 20 DAH and decreased as seeds develop. Especially, in Dongjin, the highest expression of PALs and 4CL was in 6 DAH and their expression was gradually decreased as seeds develop. These genes expression data also exhibited that, in developing seeds of I.526, phenylpropanoid pathway genes were slightly or significantly (in some genes) upregulated compared to Dongjin. Therefore, the overexpression of AhRS3 changed the expression pattern of phenylpropanoid pathway genes in I.526 developing seeds and this modification for gene expression is closely related to biosynthesis of resveratrol and piceid.

• Economic and Environmental Geology
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• v.9 no.1
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• pp.27-43
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• 1976

The flourite in Hwacheon, Hwanggangri and Keumsan district are major fluorite producing areas in Korea. The fluorite deposits of Hwacheon district are wholly fissure filling hydrothermal veins embedded in Precambrian gneiss and schists and Jurassic granites. Also some fluorite deposits are emplaced in felsite whose age is unknown. Emplacement of most fluorite veins of the district are controlled by EW fracture system. Fluorites are generally accompanied to chalcedonic quartz and also kaolinite, montmorillonite, dickite and calcite in parts. Vertical and lateral mineral zonings are not distinct. The fluorite deposits in the Hwanggangri district are wholly embedded in limestone and other calcareous sediments of Paleozoic Yeongweol Group. Most of the fluorite deposits belong to one of two categories which are steeply. dipping veins and gently dipping replacement deposits adjacent to Late Cretaceous(83-90mys) granite bodies. The strikes of fluorite veins of Hwanggangri district mostly occupy the fractures of $N30^{\circ}-40^{\circ}E$ and $N30^{\circ}-40^{\circ}W$ system. Fluorites are accompanied to calcite, milky quartz, chalcedonic quartz, and also montmorillonite, kaolinite in parts. But in some deposits, scheelite, various sulfide minerals and barite are accompanied. Emplacement of fluorite deposits are largely controlled by lithology and structures of this district. In some deposits fluorite veins gradate to scheelite veins and also telescoping of the mineral zones are found in this district. In the Keumsan district, fissure-filled fluorite veins and replacement deposits are mostly emplaced in limestone of Paleozoic Yeongweol Group, late Cretaceous quartz-porphyry, granite and sandstone. Some deposits are emplaced in Precambrian metasediments. Mineralogy and other characteristics of the deposits in this district is similar to those of Hwanggangri district. Fluid inclusion studies reveal the difference of salinities, $CO_2$ contents of ore fluid and temperatures during fluorite mineral deposition in the these districts. In Hwacheon district, ore-fluids were comparatively dilute brine and low $CO_2$ content. Filling temperatures ranges $104^{\circ}C$ to $170^{\circ}C$. In the Chuncheonshinpo mine, most deeply exploited one in this district, salinitles range 0.5-2. 2wt. % NaCl and filling temperatures range from $116^{\circ}C$ to $143^{\circ}C$. In the Hwanggangri district, ore fluids were complex and filling temperature ranges very widly. In the contact metasomatic fluorite deposits, ore fluid were NaCl rich brines with moderate $CO_2$ content and filling temperatures range from $285^{\circ}C$ to above $360^{\circ}C$. Fluids inclusions in tungsten and sulfide minerals bearing fluorite veins show high $CO_2$ content up to 31wt. %. Filling temperature ranges from $101^{\circ}C$ to $310^{\circ}C$. Fluids inclusions In mainly fluorite bearing veins were more dilute brine and low $CO_2$ contents. Filling temperatures range from $95^{\circ}C$ to $312^{\circ}C$. Filling temperature of fluid inclusions of Keumsan district are between $95^{\circ}C$ and $237^{\circ}C$. Data gathered from geologic, mineralogic and fluid inclusion studies reveal that fluorite mineralization in H wacheon district proceeded at low temperature with dilute brine and low $CO_2$ content. In Hwangganri district, fluorite mineralization proceeded by several pulse of chemically distinct ore fluids and formed the mineralogically different type of deposits around cooling granite pluton which emplaced comparatively shallow depth.

• The Journal of Engineering Geology
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• v.14 no.4 s.41
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• pp.371-379
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• 2004

Soil investigation data at 7 different locations around Honam costal region were analyzed and experimental correlations between soil properties and parameters of soft clay were presented. Most soils were classified as CL and CH by the Unified Soil Classification System and were unstable structurally because the water contents were generally greater than the liquid limits. The compression index has good correlations with water content, liquid limit and initial void ratio. The trend of these correlations were similar to the Skempton equation Cc = 0.009(LL -10) and other studies for Korean soft clays but the constants were small different. The slope of these correlations for Honam costal region were slightly greater than those for Kyunggi costal region and Kyungnam costal region. The correlation coefficient (R) between the liquid limit and the plastic index is 0.93. It is seen that not only the water content and the liquid limit but also the water content and the initial void ratio are correlate, therefore the experimental equations were presented for the practical purpose.

• Radiation Oncology Journal
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• v.20 no.2
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• pp.179-185
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• 2002

Purpose : For the purpose of quality assurance of self-developed stereotactic radiosurgery system, a multi-purpose phantom was fabricated, and accuracy of radiation dose distribution during radiosurgery was measured using this phantom. Materials and Methods : A farmer chamber, a 0.125 cc ion chamber and a diode detector were used for the dosimetry. Six MV x-ray from a linear accelerator (CL2100C, Varian) with stereotactic radiosurgery technique (Green Knife) was used, and multi-purpose phantom was attached to a stereotactic frame (Fisher type). Dosimetry was done by combinations of locations of the detectors in the phantom, fixed or arc beams, gantry angles $(20^{\circ}\~100^{\circ})$, and size of the circular tertiary collimators (inner diameters of $10\~40\;mm$). Results : The measurement error was less than $0.5\%$ by Farmer chamber, $0.5\%$ for 0.125 cc ion chamber, and less than $2\%$ for diode detector for the fixed beam, single arc beam, and 5-arc beam setup. Conclusion : We confirmed the accuracy of dose distribution with the radiosurgery system developed in our institute and the data from this study would be able to be effectively used for the improvement of quality assurance of stereotactic radiosurgery or fractionated stereotactic radiotherapy system.

• Journal of the Korean Institute of Gas
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• v.5 no.3 s.15
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• pp.45-50
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• 2001

Open rack vaporizer (ORV) has been used in liquefied natural gas (LNG) receiving terminal in order to vaporize LNG into natural gas (NG) by heat exchange with seawater The U-type ORV which had been operated with seawater for 14 years is one of the important utilities of the gas production and the weld part of tube connected with header_ pipe had experienced many corrosion problems. To elucidate the cause of corrosion at weld part of vaporizer tube, corrosion potentials were compared by parts. This study concerns on the measurement of corrosion pit depth using non-destructive method and the evaluation of stress distribution in an aspect of safety with finite element analysis. In order to confirm the reliability of galvanic corrosion between weld parts and base metal, the measurement of corrosion potential by parts was conducted for 20 minutes in 3.5$\%$(wt.) NaCl solution. Many non-destructive methods were tried to measure the remaining thickness of vaporizer tube at fields. For general corrosion, tangential radiography test was confirmed as an effective method. In case of a fine corrosion pit, the shape of corrosion pit was reproduced using surface replication method. From collected data, stress distributions were quantitatively evaluated with 2-dimensional finite element method and the diagnostic evaluation on internal pressure of the U-type vaporizer could be made.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.2
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• pp.323-327
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• 2014

This study was carried out to provide basic data on the anion, element, chromatic and antioxidant activities of commercial dried lavers cultivated from Seocheon, Wando, Goheung and Busan in Korea. The highest concentrations of several anions ($Cl^-$, $NO_3{^-}$, $SO_4{^2-}$, and $PO_4{^3-}$ per 100 g of dried laver) were 412.00 mg in Wando, 545.90 mg in Goheung, 297.35 mg in Seocheon, and 79.70 mg in Wando, respectively. The highest concentrations of N, C, and S were 6.40%, 41.36%, and 0.98%, respectively, in dried laver cultivated from Wando. Hunter's color values were significantly high in the order of Seocheon < Goheung < Busan < Wando for lightness, Wando < Busan < Goheung < Seocheon for redness, and Goheung < Seocheon < Busan < Wando for yellowness in the dried lavers. Total phenolic content and DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activities showed highest values of 166.10 mg/100 g and 28.48%, respectively, in dried laver cultivated from Wando.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.14
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• pp.5873-5878
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• 2014

Hypoxia and autophagy are known to facilitate tumor progression. We here aimed to investigate the role of hypoxia-associated autophagy in cholangiocarcinoma (CCA) survival and metastasis. Immunostaining of hypoxic-responsive proteins (HIF-$1{\alpha}$ and BNIP3) and a key regulator of autophagy (PI3KC3) were examined in CCA tissues and their expression levels were compared with clinicopathological parameters. A hypoxia mimicking condition ($CoCl_2$ treatment) was also tested regarding CCA cell functions. Our results showed that HIF-$1{\alpha}$ (66%), BNIP3 (44%) and PI3KC3 (46%) showed strong staining in human CCA tissues. Positive expression of HIF-$1{\alpha}$ (p=0.033), BNIP3 (p=0.040) and PI3KC3 (p=0.037) was significantly correlated with lymph node metastasis. HIF-$1{\alpha}$ was well associated with BNIP3 (r=0.3, p<0.01) and PI3KC3 (r=0.2, p<0.01). The survival rates of patients who were positive with HIF-$1{\alpha}$ (p=0.047) or co-expressed HIF-$1{\alpha}$ and BNIP3 (p=0.032) or HIF-$1{\alpha}$ and PI3KC3 (p=0.043) were significantly greater than in the negative groups. CCA cells treated with $CoCl_2$ showed an increase in HIF-$1{\alpha}$, BNIP3, PI3KC3 and LC3-II, with increased cell migration and pFAK levels. These data suggest that hypoxia associated autophagy enhances CCA metastasis, resulting in a poor prognosis of CCA.

• Journal of the Korean Institute of Landscape Architecture
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• v.16 no.2
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• pp.1-7
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• 1988

Predicting treeform should be very important, eventhough it is difficult, step for landscape designers. This study was carried out to develop a method to classify the treeforms of landscape plants by numerical data. Twenty five treeforms are selected to be compared with 41 Zelkova serrata MAKINO, 26 Pinus strobus L., and 44 Magnolia denudata DESROUX. Statistical judgement was made by using X$^2$-Test. The results are summerized as follows. 1. Zelkova serrata was classified as B3 or C3. This species showed a tendency of changing from A type to B or C type, and the CL/CH ratio(canopy length/canopy height) is thought to be increased as aged. 2. pinus strobus was classified as D4-D5 and the CL/CH ratio tends to be increased as aged. 3. Magnolia denudata was classified as D4 and the change by aging was not observed clearly.