• Journal of Microbiology and Biotechnology
• /
• v.14 no.6
• /
• pp.1286-1294
• /
• 2004

In a previous study by the current authors, hepatocellular carcinoma (HCC) was determined to be epidemiologically sex-dependent, and the incidence and multiplicity of HCC found to decrease in estradiol-3 benzoate (EB)-treated F344 rats. Therefore, to ascertain the anticancer mechanism of EB, a commercially available cDNA microarray, with a total of 14,815 cDNA rat gene clones, was used to determine the differentially expressed genes in nontreated livers, EB-treated livers, and diethynitrosolamine (DEN)-induced HCC. In the sequenced experiment, a total of 85 genes were differentially expressed at either two or more times the rate of the normal expression, where 33 genes were downregulated by EB, and 52 genes upregulated. Candidate genes were selected according to significant changes observed in the mRNA expression in the EB-treated livers compared with the nontreated livers, then these genes were filtered according to their different expression patterns in the DEN-induced tumors compared to the estrogen-treated livers. To confirm the microarray data, a real-time PCR analysis was performed for ten selected genes: the H-ras revertant protein 107 (H­rev107), insulin-like growth factor binding protein (lOFBP), parathyroid hormone receptor (PI'HR), SH3 domain binding protein (SH3BP), metallothionein, src-suppressed C-kinase substrate (SSeCK) gene, phosphodiesterase I, CD44, epithelial membrane protein 3 (EMP3), and estrogen receptor a (ERa). The SSeCK and phosphodiesterase I genes were both upregulated in the DEN-induced hepatocarcinomas, yet their possible carcinogenic functions remain unknown. Meanwhile, the other genes were downregulated, including the genes related to growth regulation (IOFBP, H-revI07, ER$\alpha$), adipogenesis inhibition (PTHR), and tumor suppression (metallothionein).

• Journal of Veterinary Clinics
• /
• v.25 no.4
• /
• pp.245-248
• /
• 2008

Herbal medicines are widely used to treat a variety of human diseases. However, therapeutic effect of herbal medicine on the canine facial nerve paralysis (FNP) has not been investigated. This study examined therapeutic effects of Oyaksungisan on the canine FNP. Ten dogs with the induced canine FNP were equally divided into the control and an herbal medicine-treated groups (5 dogs, Oyaksungisan-treated group), respectively. No treatment was given to the control group. In the Oyaksungisan-treated group, Oyaksungisan was administered orally, twice per day for 2 weeks (50 mg/kg) after inducing FNP. Significant differences in the clinical scores were detected between the Oyaksungisan-treated and control groups on day 14 (p < 0.05). The serum creatine kinase (CK) activities in the Oyaksungisan-treated group showed a tendency to decrease but there was no significant difference compared with those of the control group. In conclusion, Oyaksungisan was effective for the induced canine FNP.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2008.04a
• /
• pp.373-376
• /
• 2008

Ultra High Strength Concrete(UHSC) is necessary a clear presentation about mechanical property that is different from normal strength concrete and an evaluation of serviceability of high rise building which is used ultra high strength concrete. To mixing ultra high strength concrete with $f_{ck}$=150MPa pre-mix cement were manufactured and experimental study were conducted to evaluated on the mixing properties and compressive strength with major variables as unit cement contents, water-binder ratio and type of pre-mix cement. As a test result, it is shown that the concrete mixing time is required about 5$^{\sim}$6 minute untill the each materials(ordinary portland cement, silica fume, blast-furnace slag powder and anhydrite) are revitalized enough. A slump flow of fresh concrete are shown about 700$^{\sim}$750mm with proper viscosity. And average value of concrete compressive strength are shown about 77% in 7days, 87% in 14days and 102% in 56days for 28days of concrete material age. From this experimental study, a proper mixture proportion of pre-mix cement are recommended about 54$^{\sim}$59% OPC, 25$^{\sim}$30% blast-furnace slag powder and 10$^{\sim}$15% silica fume for mix the ultra high strength concrete with $f_{ck}$=150MPa.

• Journal of Veterinary Clinics
• /
• v.24 no.4
• /
• pp.503-508
• /
• 2007

Although canine facial nerve paralysis(FNP) occurs similarly in humans, there is no properly recognized therapy using Western medicine for idiopathic causes. To elucidate therapeutic measures by acupuncture(AP) on canine FNP, we examined the therapeutic effect of injection-AP on the artificially induced canine FNP. Twelve dogs on artificially induced canine FNP were divided into a control group(4 dogs), an experimental dexamethasone-treated group(dexamethasone group, 4 dogs) and an experimental bee venom-treated group(apitoxin group, 4 dogs). Saline (1 ml) was intramuscularly injected into the head muscle after the induction of FNP in the control group. On the other hand, injection-AP with dexamethasone was performed on such acupoints as LI04, LI20, ST02, ST07, TH17, SI18, GB03 and GB34, twice per week after induction of FNP in the dexamethasone group. In addition, injection-AP with $100{\mu}g$ of apitoxin was performed on the same acupoints as the dexamethasone group twice per week after the induction of FNP in the apitoxin group, respectively. The changes of the clinical symptoms of FNP with each treatment during the experimental period were recorded by using clinical scores, respectively. The changes of serum creatine kinase(CK) activities along with each treatment were determined using an autoanalyzer. The significant differences of clinical scores were detected on day 14(p<0.05) in the apitoxin and dexamethasone groups, compared with those in the control group, respectively. However, significant difference was not detected between the apitoxin and dexamethasone groups. Significant differences of serum CK activities were detected on day 7(p<0.05) and day 14(p<0.05) in the dexamethasone and apitoxin groups, compared with those in the control group, respectively. However, significant difference was not detected between the dexamethasone and apitoxin groups. In condition, injection-APs with apitoxin and dexamethasone were all effective for treatment of canine FNP and the therapeutic effect by injection-AP with apitoxin was similar to that of injection-AP with dexamethasone.

• Journal of Chest Surgery
• /
• v.49 no.6
• /
• pp.435-442
• /
• 2016

Background: Blunt traumatic cardiac rupture is rare. However, such cardiac ruptures carry a high mortality rate. This study reviews our experience treating blunt traumatic cardiac rupture. Methods: This retrospective study included 21 patients who experienced blunt traumatic cardiac rupture from 1999 to 2015. Every patient underwent surgery. Several variables were compared between survivors and fatalities. Results: Sixteen of the 21 patients survived, and 5 (24%) died. No instances of intraoperative mortality occurred. The most common cause of injury was a traffic accident (81%). The right atrium was the most common location of injury (43%). Ten of the 21 patients were suspected to have cardiac tamponade. Significant differences were found in preoperative creatine kinase-myocardial band (CK-MB) levels (p=0.042) and platelet counts (p=0.004) between the survivors and fatalities. The patients who died had higher preoperative Glasgow Coma Scale scores (p=0.007), worse Trauma and Injury Severity Scores (p=0.007), and higher Injury Severity Scores (p=0.004) than those who survived. Conclusion: We found that elevated CK-MB levels, a low platelet count, and multi-organ traumatic injury were prognostic factors predicting poor outcomes of blunt cardiac rupture. If a patient with blunt traumatic cardiac rupture has these factors, clinicians should be especially attentive and respond promptly in order to save the patient's life.

• Preventive Nutrition and Food Science
• /
• v.15 no.2
• /
• pp.88-91
• /
• 2010

This study examined the effects of Korean red ginseng (RG) on muscle injury induced by eccentric exercise. Eighteen college male students were randomly assigned to either the RG group or the placebo group (P). The RG group ingested RG extract at 20 g/day (mixed with 200 mL of water) for 7 days prior to performing a downhill treadmill exercise and for 3 days after performing the downhill treadmill exercise, while the P group ingested 200 mL of water containing Agastachis Herba for 7 days prior to and 3 days following a downhill treadmill exercise. All subjects performed 2 bouts of a downhill treadmill exercise (6 km, -14 degree slope, 12 km/hr speed) with a 5 minute resting interval between bouts. Blood samples were drawn immediately before and after exercise, and at 1 hr, 24 hr, 48 hr, and 72 hr. Two-way repeated ANOVA documented that creatine kinase (CK) levels in the RG group were significantly reduced at 48 hr after exercise, as compared with P group, and remained constant until 72 hr after exercise. Lactate levels in the RG group were also significantly lower at 24 hr and 72 hr after exercise as compared to the P group. We conclude that supplementation of RGreduces CK levels and may prevent muscle injury induced by eccentric exercise.

• The Korean Journal of Food And Nutrition
• /
• v.14 no.5
• /
• pp.411-417
• /
• 2001

This study was designed to observe the effects of edible mugwort and medicinal mugwort ( Ganghwa medicinal mugwort) on blood components of rats. Eighteen young rats of Sprague Dawley strain that weighted 84${\pm}$9g, were used in this study. They were fed on the basal diet(control diet). 5% edible mugwort powder(EM diet) and 5% medicinal mugwort(MM diet) for 4 weeks respectively. In proximate composition of mugworts, crude protein and crude ash contents of EM were 2% higher than those of MM. Mn, Zn and vitamin A contents of EM were 3 times higher than those of MM, but in Ca content, MM was 5 times higher than that of EM. The contents of albumin and the activity of ALT, AP amylase and CK in serum were significantly increased, but the activity of AST was significantly lowered in EM and MM diet groups. The contents of TG and the activity of LDH were towered in EM diet group and MM diet group resulted in decrease of glucose content and increase of TP content.

• Journal of the Korea Concrete Institute
• /
• v.17 no.4 s.88
• /
• pp.535-542
• /
• 2005

Two series of experiments on the performance of beam-column joints in High-Strength Reinforced concrete frames were carried out. Main experimental parameters were : concrete strength, column axial load and amount of joint hoop reinforcement. Test result showed that the ultimate shear strength of exterior joints increased of column axial compressive force and the amount of the joint hoop reinforcements. Through the regression analysis on the 24data, the following equation is obtained $jv_u=(2.935{\times}10-3\;{\rho}jw{\cdot}fy\;+\;0.365){\sqrt{f_{ck}}}$

• Journal of Korean Society for Atmospheric Environment
• /
• v.32 no.2
• /
• pp.184-192
• /
• 2016

Recently, studies on reducing mercury have been actively conducted worldwide, which include the current status of mercury emissions and mercury control technology. Among the control technology, Sorbent Trap measurement method has been aggressively developed due to its reliability, easiness in measurement and analysis. The purpose of this study is to evaluate the applicability of the new international measurement method; Sorbent Trap. For this, the study compared the Sorbent trap method (US EPA Method 30B) and the Korean Standard Method for Examination of Air (ES 01408.1) to evaluate their reliability, and developed mercury emission factors. As the result, the relative standard deviations (% RSD) of the two methods were 3.5~13.4% at Coal-fired Power Plants (CPP), 4.0~18.4% at Cement Kilns (CK), and 3.0~11.3% at Medical Waste Incinerators (MWI). The emissions factors were developed as 14.50 kg/ton at CPP, 45.10 kg/ton at CK, and 1,290.2 kg/ton at MWI.

• Natural Product Sciences
• /
• v.28 no.2
• /
• pp.68-74
• /
• 2022

HemoHIM was used as a Korean traditional medicine for anti-inflammatory and antioxidant effects. However, there is no study on the effect of HemoHIM on fatigue. We examined the potential use of HemoHIM to determine whether it can induce anti-fatigue effects. Mice were administered with HemoHIM and VEH for 14 days. On the last day of treatment, mice were subjected to behavioral tests. Subsequently, their plasma and muscle were collected after the treadmill test to measure lactate, lactate dehydrogenase (LDH), ammonia, corticosterone, glycogen, and creatine kinase (CK). We found that HemoHIM moderately increased the running time (s) in the treadmill and mobility duration in the cold swimming tests. In addition, the VEH group showed a significant increase in lactate, LDH, and corticosterone levels in the plasma compared to the group that did not perform the test. However, this was moderately reduced in HemoHIM treatment. Moreover, the HemoHIM-treated group showed significant differences in LDH and glycogen levels, and showed significantly different CK levels in the muscle. HemoHIM is considered to be effective in improving fatigue, given the duration of cold swimming or running time on a treadmill. Also, HemoHIM treatment resulted in reduced concentrations of blood and muscle parameter analysis.