• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.2
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• pp.136-143
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• 1999

To understand the growth of attached microalgae to the immersed artificial surfaces in seawater with exposure time, chlorophyll a (chl a) concentration and abundance of attached microalgae to glass slides, and primary productivity and chl a concentration on coverglasses were investigated in Incheon Harbour in May, June 1996 and January-February 1997. Chl a concentrations of microalgae and abundances of diatoms attached to glass slides reached 62.5 mg chl a $m^{-2}$ and $144{\times}10^3$ cells $cm^{-2}$, respectively, during the study period. Chl a concentrations increased with exposure time, and they were significantly correlated with the abundances of attached diatoms ($r^2=0.79$, p<0.001). The chl a concentrations of attached micro algae on coverglass reached the maximum values of 31.1 mg chl a $m^{-2}$ and 65.4 mg chl a $m^{-2}$, and then decreased in May, June 1996. But in January-February 1997, the chl a concentration increased continuously up to 98.9 mg chl a $m^{-2}$. The primary productivity reached the maximum values of 63.1 mgC $m^{-2}\;h^{-1}$, 347.0 mgC $m^{-2}\;h^{-1}$ and 78.3 mgC $m^{-2}\;h^{-1}$, respectively, in May, June and January-February. The primary productivity in May and June varied in accordance with chl a concentrations. But in January-February, the primary productivity decreased from 26 days of exposure while chl a concentration continued to increase. Two cases that primary productivity decreased abruptly seemed to be caused by decrement of chl a and light specific $P^B$ (chl a specific primary productivity) (May and June) and by decrement of light specific $P^B$ due to photoinhibition (January-February). The results of present study indicated that chl a concentrations and the primary productivity of microalgae attached to artifical surfaces immersed in seawater would expedite analysis of dynamics of biomass and physiological status of attached microalgae during biofilm formations.

• Korean Journal of Ecology and Environment
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• v.41 no.4
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• pp.512-518
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• 2008

This study were to evaluate trophic conditions, N : P ratios, and empirical relations of chlorophyll (CHL) systematically using TN, TP, and CHL values in agricultural reservoirs and drinking water supplying dams. During the study, nutrients and CHL varied depending on seasonal conditions and types of the reservoirs, but most reservoirs were diagnozed as eutrophic to hypertrophic. Mass ratios of TN : TP averaged 93.1 (range: $0.68{\sim}1342$) and about 96.6 % of the total observations (n=516) was > 17 in the N : P ratios. This result suggests that P was a potential factor limiting algal growth in the entire reservoir. Thus, TN : TP ratios were a function of phosphorus rather than nitrogen. Regression analysis of log-transformed N : P ratios against TP in DWDRs and ARs showed that ratios were linearly declined with an increase of TP ($R^2$>0.66; p<0.001). Seasonal mean CHL was minimum ($4.3{\mu}g\;L^{-1}$, range: $0.1{\sim}39.7{\mu}g\;L^{-1}$) in premonsoon, and was similar between the monsoon and postmonsoon. In contrast, one of the tremendous features was that values of CHL was greater in the ARs than DWDRs. Thus, the spatial and temporal patterns in CHL were similar to those of TP but not TN. Empirical models of CHL-TP showed that CHL variation could explain average 15.3% and 11.3% in DWDRs and ARs, respectively. Seasonal analysis of empirical models showed that CHL-TP relations were stronger in postmonsoon than those of premonsoon and monsoon.

• Korean Journal of Ecology and Environment
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• v.45 no.1
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• pp.82-92
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• 2012

In this study, we identified spatial and temporal patterns of reservoir trophic state within Keum-river watershed and analyzed correlations between chlorophyll-$a$ (Chl-$a$) and water quality parameters including conductivity and total phosphorus (TP). The reservoirs were separated into three trophic categories by the criteria of TP: 2 oligotrophic (9.3~9.4 ${\mu}g\;L^{-1}$), 15 mesotrophic (10.3~19.2 ${\mu}g\;L^{-1}$), and 14 eutrophic reservoirs (38.9~117.1 ${\mu}g\;L^{-1}$). Water quality parameters such as conductivity, TP, and Chl-$a$ reflected rainfall patterns, and the patters of annual mean TP were similar to the variation of annual mean Chl-$a$. Empirical models of Chl-$a$ against TP in reservoirs showed that statistical significance ($p$ <0.05) occurred in only some seasons and the trophic relations were modified by a washing-out effect or high non-algal light attenuation.

• Molecules and Cells
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• v.40 no.10
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• pp.773-786
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• 2017

The loss of green coloration via chlorophyll (Chl) degradation typically occurs during leaf senescence. To date, many Chl catabolic enzymes have been identified and shown to interact with light harvesting complex II to form a Chl degradation complex in senescing chloroplasts; this complex might metabolically channel phototoxic Chl catabolic intermediates to prevent oxidative damage to cells. The Chl catabolic enzyme 7-hydroxymethyl Chl a reductase (HCAR) converts 7-hydroxymethyl Chl a (7-HMC a) to Chl a. The rice (Oryza sativa) genome contains a single HCAR homolog (OsHCAR), but its exact role remains unknown. Here, we show that an oshcar knockout mutant exhibits persistent green leaves during both dark-induced and natural senescence, and accumulates 7-HMC a and pheophorbide a (Pheo a) in green leaf blades. Interestingly, both rice and Arabidopsis hcar mutants exhibit severe cell death at the vegetative stage; this cell death largely occurs in a light intensity-dependent manner. In addition, 7-HMC a treatment led to the generation of singlet oxygen ($^1O_2$) in Arabidopsis and rice protoplasts in the light. Under herbicide-induced oxidative stress conditions, leaf necrosis was more severe in hcar plants than in wild type, and HCAR-overexpressing plants were more tolerant to reactive oxygen species than wild type. Therefore, in addition to functioning in the conversion of 7-HMC a to Chl a in senescent leaves, HCAR may play a critical role in protecting plants from high light-induced damage by preventing the accumulation of 7-HMC a and Pheo a in developing and mature leaves at the vegetative stage.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.3
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• pp.183-188
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• 2001

The deposition behavior and photoelectric response characteristics of chlorophyll a(Chl a) monolayers and multilayers were investigated under various film fabrication conditions. Chl a LB films were deposited onto quartz and pretreated ITO glass substrates under several fabrication conditions, including surface pressure and number of layers. The absorption spectra of Chl a in a solution state and solid-like state (LB films) were fairly consistent with each other, and two absorption peaks were found at 678 and 438nm, respectively. The prepared Chl a LB films were set into an electrochemistry cell equipped with a Pt plate as the counter electrode, and the photoelectric response characteristics were obtained and analyzed relative to the light illumination. By considering the resulting photocurrents, the optimal fabrication conditions for Chl a LB films were determined as 20mN/m of surface pressure and 20 layers. The action spectrum of the Chl a LB films was obtained in the visible region, and was found to be in good agreement with the absorption spectrum. The possible application of the proposed system as a constituent of an artificial color recognition device was suggested based on combining with the photoelectric conversion property of another light-sensitive biological pigment.

• Environmental Engineering Research
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• v.23 no.4
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• pp.364-373
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• 2018

The present study assessed the efficient removal of nutrients and Chlorophyll-a (Chl-a) by using methyl esterified sericite (MES) and pine needle extracts (PNE), a low cost and abundant green hybrid material from nature. For this purpose, the optimal conditions were investigated, such as the pH, temperature, MES and PNE ratio, and MES-PNE dose. In addition, a Microcystis aeruginosa control using MES-PNE was also analyzed with various inhibition models. The removal of the nutrient and Chl-a onto MES-PNE was optimized for over 95% removal as follows: 2-2.5 for the MES-PNE ratio, 7-8 pH and a $22-25^{\circ}C$ temperature. In this respect, approximately 1.52-2.20 g/L of MES-PNE was required to remove each 1 g of dry weight/L of Chl-a. Total phosphorus (TP) has a greater influence on the increase in Chl-a than total nitrogen (TN) according to the correlation between TN, TP and Chl-a. Moreover, the Luong model was the best model for fitting the biodegradation kinetics data from Chl-a on MES-PNE from lake water. The novel hybrid material MES-PNE was very effective at removing TN, TP and Chl-a from the lake and can be applied in the field.

• Fisheries and Aquatic Sciences
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• v.22 no.1
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• pp.2.1-2.9
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• 2019

In the present study, the protective effects of Hizikia fusiforme and Chlorella sp. extracts on lead acetate-induced hepatotoxicity were investigated. Hepatic damage was induced in rats by intraperitoneal (i.p.) injection of lead acetate and the protective effects of H. fusiforme (HZK) and Chlorella sp. (CHL) extracts on lead acetate-induced hepatic damage in rat liver were examined. The results revealed significantly increased glutamic oxaloacetate and glutamic pyruvic transaminase levels in the group treated with lead acetate only (Pb group); oral administration of HZK and CHL extracts tended to decrease the enzyme levels similar to those observed in the control group. Regarding antioxidant enzymes, superoxide dismutase activity was increased in the Pb group and decreased in a concentration-dependent manner in the HZK- and CHL-treated groups. Glutathione levels were increased in a concentration-dependent manner in the HZK- and CHL-treated groups. There was no significant difference in catalase activity. Western blot analysis showed inflammation-related protein expression in mitogen-activated protein kinase and Nrf2 pathways was affected in the HZK- and CHL-treated groups. Therefore, HZK and CHL extracts exerted antioxidant and anti-inflammatory effects against lead acetate-induced hepatotoxicity. Development of functional health foods containing HZK and CHL extracts, which have hepatoprotective effects against inhaled lead acetate, should be considered.

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.499-506
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• 2013

To estimate the effect of algae to particulate organic matter in the upper regions of brackish Lake Sihwa, temporal and spatial variations of particulate organic carbon (POC) and phytoplankton pigments (chlorophyll a; Chl-a, pheophytin-a; Pheo-a), and their relationships were studied at seven sites of the brackish regions from March to October 2005 and 2006. POC concentration varied from 1.0 to $76.6mgL^{-1}$ (mean $7.4mgL^{-1}$), with maximal concentrations occurring in the middle parts of the study area in spring of 2005 and 2006. Concentrations of Chl-a and Pheo-a varied from 1.3 to $942.9{\mu}gL^{-1}$ (mean $71.0{\mu}gL^{-1}$) and $1.4{\sim}1,545.5{\mu}gL^{-1}$ (mean $59.9{\mu}gL^{-1}$), respectively, and corresponded closely with variation in POC. During the study period Pheo-a concentration was 44.2% of total Chl-a, implying that non-living or inactive phytoplankton is also the important part of phytoplankton-derived POC in brackish regions of Lake Sihwa. From the positive linear relationships between POC and phytoplankton pigments (POC with Chl-a (r=0.93), total Chl-a (r=0.88), and Pheo-a (r=0.81)), it is suggested that phytoplankton was a significant component of POC in the upper regions of brackish Lake Sihwa. On the other hand, the ratios of POC/Chl-a and POC/total Chl-a (Chl-a+Pheo-a) were 82.9 and 35.9, respectively. The ratio of POC/total Chl-a is similar to those reported in previous studies, including 40~60 in estuaries. This study suggests that Pheo-a concentration is considered in estimation of POC concentration from phytoplankton pigments in aquatic systems with high content of Pheo-a, like an upper region of blackish Lake Sihwa.

• Journal of Environmental Impact Assessment
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• v.26 no.6
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• pp.470-478
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• 2017

This study was classified into two groups, normal season group and drought season group, by the cluster analysis using the weather and water quality data from 2012 to 2015, using SPSS 18 version. Also each cluster was classified into three spaces, Gangcheon, Yeoju and Ipoh weir. We performed the multiple regression analysis with each monthly data that concentration of Chl-a was more than algae warming level. 6 groups classified in time and space were analyzed by the correlation analysis between concentration of Chl-a and 3 weather, 11 water quality and discharge factors. We developed Chl-a prediction equations of each group with independent variables of the multiple regression analysis applying to the correlation result. The result of cluster analysis was that the period was divided into two groups, normal group(2012-2013) that total annual precipitation rate was normal and drought group(2014-2015) that total annual precipitation rate was less than 1,000 mm/hr, in time. The months that concentration of Chl-a was more than algae warming level in each group classified by cluster analysis were that the normal group was 3~8 and drought group was 3 and 6~10. The correlation result between Chl-a and weather, water quality and discharge factors for each 6 group was that relationships between Chl-a and water, discharge factors were high in the drought group more than in normal group at all weirs. This was influenced by velocity reduction and increasing HRT according to the intense drought. Weather, water quality and discharge factors that were high correlation with Chl-a were applied to independent variables of Chl-a prediction equations and each equations were developed. Among them, Each adjusted R square of Prediction equations for Chl-a in each group at Ipoh weir where is located in Namhan river downstream and is directly connected to Paldang dam were normal group = 0.920 and drought group = 0.818. It's showed the high linear.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.605-619
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• 2019

The objective of this study was to analyze eutrophication characteristics, empirical model analysis, and variation of water quality according to monsoon intensity in Hapcheon Reservoir for 16 years from 2002 to 2017. Long-term annual water quality analysis showed that Hapcheon Reservoir was in a meso-nutrition to eutrophic condition, and the eutrophic state intensified after the summer monsoon. Annual rainfall volume (high vs. low rainfall) and the seasonal intensity in each year were the key factors that regulate the long-term water quality variation provided that there is no significant change of the point- and non-point source in the watershed. Dry years and wet years showed significant differences in the concentrations of TP, TN, BOD, and conductivity, indicating that precipitation had the most direct influence on nutrients and organic matter dynamics. Nutrient indicators (TP, TN), organic pollution indicators (BOD, COD), total suspended solids, and chlorophyll-a (Chl-a), which was an estimator of primary productivity, had significant positive relations (p<0.05) with precipitation. The Chl-a concentration, which is an indicator of green algae, was highly correlated with TP, TN, and BOD, which differed from other lakes that showed the lower Chl-a concentration when nutrients increased excessively. Empirical model analysis of log-transformed TN, TP, and Chl-a indicated that the Chl-a concentration was linearly regulated by phosphorus concentration, but not by nitrogen concentration. Spatial regression analysis of the riverine, transition, and lacustrine zones of $log_{10}TN$, $log_{10}TP$, and $log_{10}CHL$ showed that TN and Chl-a had significant relations (p<0.005) while TN and Chl-a had p > 0.05, indicating that phosphorus had a key role in the algal growth. Moreover, the higher correlation of both $log_{10}TP$ and $log_{10}TN$ to $log_{10}CHL$ in the riverine zone than the lacustrine zone indicated that there was little impact of inorganic suspended solids on the light limitation in the riverine zone.