• Mycobiology
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• v.43 no.1
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• pp.24-30
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• 2015

The effects of monokaryotic strains on fruiting body formation of Lentinula edodes were examined through mating and cultivation of the mated dikaryotic mycelia in sawdust medium. To accomplish this, monokaryotic strains of L. edodes were isolated from basidiospores of the commercial dikaryotic strains, Chamaram (Cham) and Sanjo701 (SJ701). A total of 703 matings (538 self-matings and 165 outcrosses) were performed, which generated 133 self-mates and 84 outcross mates. The mating rate was 25% and 50% for self-mating and outcross, respectively. The bipolarity of the outcross indicated the multi-allelic nature of the mating type genes. The mating was only dependent on the A mating type locus, while the B locus showed no effect, implying that the B locus is multi-allelic. Next, 145 selected dikaryotic mates were cultivated in sawdust medium. The self-mated dikaryotic progenies showed 51.3% and 69.5% fruiting rates for Cham and SJ701, respectively, while the fruiting rate of the outcross mates was 63.2%. The dikaryotic mates generated by mating with one of the monokaryotic strains, including A20, B2, E1, and E3, showed good fruiting performance and tended to yield high fruiting body production, while many of the monokaryotic strains failed to form fruiting bodies. Overall, these findings suggest that certain monokaryotic strains have traits enabling better mating and fruiting.

• Korean Journal of Materials Research
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• v.26 no.10
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• pp.528-534
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• 2016

The aim of this paper is to consider the effect of the manufacturing processes on corrosion and centerline cracking of ancient bronze spoons. The ancient bronze spoons in question were made by several steps of forging, in reheated condition with cast ingots. The manufacturing method is similar to that of the modern spoons. The investigations include observations from light and scanning electron microscopes of the microstructure in terms of the crack propagation. Cracks in the centerline are caused by solute segregation in the center-line region; this solute is solidified in the final stage of bronze spoon manufacture. Centerline cracking is also caused by ${\alpha}$ phase segregation, accompanied by forged overlapping along the longitudinal direction of the spoons. A vertical stripe with cracks along the centerline of the spoon's width is formed by folding in the wrought process. The overlapping area causes crack propagation with severe corrosion on the spoon surfaces over a period of a thousand years. The failure mechanisms of ancient bronze spoons may be similar to that of modern spoons, and the estimation of the failure mechanisms of ancient spoons can be appropriate to determine failure causes for such modern spoons.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.634-643
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• 2021

Han In-jin (Artemisia iwayomogi Kitamura) and Cham Dang-gwi (Angelica gigas Nakai) exhibit antibacterial, antiparasitic, antifungal, and antiviral properties in vitro. In this study, mixture of the extracts of these two medicinal plants was absorbed on pellets. Thereafter, these pellets were fed to olive flounder Paralichthys olivaceus for 12 weeks at laboratory (1^st experiment) and 24 weeks at field test (2^nd experiment), and the immune activity and disease resistance properties of the extracts were examined. It was observed that lysozyme activities of plasma, spleen, and kidney improved after 12 weeks. Furthermore, when the olive flounders were artificially infected with bacterial pathogens, their cumulative mortality decreased in the group that was fed the extracts for 12 weeks compared to that in control group, and the relative percent survival also improved. This study concluded that mixture of Han In-jin and Cham Dang-gwi extracts provides disease resistance in vivo.

• Journal of Species Research
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• v.10 no.3
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• pp.246-254
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• 2021

Subalpine and alpine ecosystems are especially vulnerable to temperature increases. Betula ermanii Cham. (Betulaceae) is a dominant broad-leaved tree species in the subalpine zone and is designated as a 'Climate-sensitive Biological Indicator Species' in South Korea. This study aimed to predict the potential distribution of B. ermanii under current and future climate conditions in South Korea using the MaxEnt model. The species distribution models showed an excellent fit (AUC=0.99). Among the climatic variables, the most critical factors shaping B. ermanii distribution were identified as the maximum temperature of warmest month (Bio5; 64.8%) and annual mean temperature (Bio1; 20.3%). Current potential habitats were predicted in the Baekdudaegan mountain range and Mt. Hallasan, and the area of suitable habitat was 1531.52 km², covering 1.57% of the Korean Peninsula. With global warming, future climate scenarios have predicted a decrease in the suitable habitats for B. ermanii. Under RCP8.5-2070s, in particular, habitat with high potential was predicted only in several small areas in Gangwon-do, and the total area suitable for the species decreased by up to 97.3% compared to the current range. We conclude that the dominant factor affecting the distribution of B. ermanii is temperature and that future temperature rises will increase the vulnerability of this species.

• Journal of dental hygiene science
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• v.24 no.1
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• pp.54-61
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• 2024

Background: The Korean name for Angelica gigas Nakai (AGN) is Cham-dang-gui, which grows naturally or is cultivated, and its dried roots are used in traditional herbal medicines. The AGN root exert various pharmacological effects. Despite the various pharmacological effects of the AGN root, there are no reports on its anti-oral microbial effects. The purpose of this study was to reveal the anti-oral microbial effect and the microbial and biochemical changes in oral microorganisms according to the concentration of the ethanol extract of AGN (EAGN) root, and to confirm the possibility of using EAGN as a plant-derived functional substance for controlling oral infectious microorganisms. Methods: Disk diffusion test, growth measurement, biofilm formation assay, and measurements of acid production and buffering capacity were performed to confirm the antibacterial effect of EAGN. Results: EAGN showed anti-oral bacterial effects against Streptococcus mutans and Aggregatibacter actinomycetemcomitans at all concentrations, with S. mutans showing a more susceptible effect at concentrations above 5.0 mg/ml and A. actinomycetemcomitans at 3.75 mg/ml. EAGN treatment significantly reduced A. actinomycetemcomitans growth at all concentrations tested. Biofilm formation was significantly reduced at concentrations above 3.75 mg/ml for S. mutans and 2.5 mg/ml for A. actinomycetemcomitans. Acid production in S. mutans and A. actinomycetemcomitans was significantly increased by treatment with EAGN, and the buffering capacities of S. mutans and A. actinomycetemcomitans increased from an EAGN concentration of 3.75 mg/ml and above. Conclusion: EAGN showed anti-oral bacterial effects against both S. mutans and A. actinomycetemcomitans at concentrations above 3.75 mg/ml, which were thought to be related to the inhibition of their growth and biofilm formation. Therefore, EAGN can be used as a safe functional substance derived from medicinal plants owing to its antibacterial effects against S. mutans and A. actinomycetemcomitans.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.1
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• pp.52-56
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• 1985

For the fundamental studies of radiation breeding in edible marine algae, the biological effects on conchospores of Porphyra species by gamma-irradiation were examined. Two varieties, Keun-cham-gim (Porphyra tenera Kjell. form tamatsuensis Miura) and Saga No.5, were chosen for this study, and their conchospores after r-irradiation($5.0{\sim}20.0$ KR) were cultured for 50 days. The results obtained were summarized as follows. 1. Gamma-irradiation in less than the dose of 20KR did not affect germination of conchospores, and almost all spores grew into two cells germ in 24 hours after irradiation, but withering germs were gradually increased in number according to higher exposure within 5 days old culture. 2. High irradiation caused the induction of giant cells, abnormal useless growth of hold-fast, lumpish thalli and callus-like lumpy tissues. 3. The liberation of neutral spores from young germs and carpospores from mature thalli were observed on the frond exposed at $10{\sim}20$ KR irradiation. All spores were normal in division and its size. 4. The best irradiation effect on growth of Keun-cham-gim was observed at 10 KR dose, whose growth-rates were $140\%$ in wet weight and $108\%$ in mean frond area, but only $48\%$ was recorded in wet weight at 20 KR exposure. Saga No.5 were in contrast with Keun-cham-gim, and their most growth-rate was $400\%$ in wet weight ($258\%$ in frond area) at 10 KR irradiation and the worst was $20\%$ at the dose of 20 KR. 5. The withering phenomenon to death by treatment of gamma-ray presented substantial difference between two varieties. Survival rate compared with control in Keun-cham-gim was $70.7\%$ at 20 KR, but that in Saga No.5 recorded $47.0\%$ at same dose. 6. Synthesizing the results of high and low r-irradiation, it was suggested tat high r-irradiation in more than 5.0 KR inhibited conspicuously the growth of germs derived from conchospores, and about half of them withered at 15.0 KR dose or more.

• Journal of Korean Medical classics
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• v.22 no.1
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• pp.27-34
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• 2009

In "Maekyojeongmiron(脈要精微論)", Jangbu(臟腑) is assigned to Chon.Gwan.Cheok(寸關尺) in according to the position. Kidney[腎] is assigned to the backward area of Cheok. Abdominal cavity[腹中] is assigned to the middle area of Cheok. Hypochondrium[季脇] is assigned to the forward area of Cheok. Liver[肝] is assigned to the backward area of left Gwan. Diaphragm[鬲] is assigned to the forward area of left Gwan. Stomach[胃] is assigned to the backward area of right Gwan. Spleen[脾] is assigned to the forward area of right Gwan. Heart[心] is assigned to the backward area of left Chon. Central part of the chest[膻中] is assigned to the forward area of left Chon. Lung[肺] is assigned to the backward area of right Chon. Thoracic cavity[胸中] is assigned to the forward area of right Chon. This method of assignment is simple and useful in clinic.

• Proceedings of the Korean Fiber Society Conference
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• 2007.11a
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• pp.449-450
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• 2007

• Proceedings of the Korean Fiber Society Conference
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• 2007.11a
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• pp.365-366
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• 2007

• Proceedings of the Korean Fiber Society Conference
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• 2007.11a
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• pp.519-520
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• 2007

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