• 제목/요약/키워드: CFA

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Mannose-resistant Hemagglutination(MRHA) 및 Colonization Factor Antigen I(CFA I)을 표현하는 대장균의 장관내 존재와 설사증 발현과의 관계 (Gastrointestinal Carriage of Escherichia coli with Hemagglutination Activity and Colonization Factor Antigen I and its Relation to Diarrhea)

  • 노승현;김경희;조양자;서인수
    • 대한미생물학회지
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    • 제22권2호
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    • pp.147-153
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    • 1987
  • Colonization factor antigen I(CFA I) has been shown to be one of several virulence factors that promote attachment of enterotoxigenic E. coli(ETEC) to small intestinal epithelial cells of humans. The ability of ETEC to produce mannose-resistant hemagglutination(MRHA) of human blood group A has been used to detect CFA I. To determine gastrointestinal carriage in Korean children of E. coli with MRHA and CFA I, 116 strains of E. coli from diarrheal children admitted to Hanyang University Hospital were examined for MRHA of human erythrocytes and the presence of CFA I. Of 45 ETEC strains, 18(40%) gave a positive MRHA($MRHA^+$) and eight(18%) were positive for CFA I(CFA $I^+$). ETEC with CFA I were all heat-stable enterotoxin(ST) producers and two of these strains were of serogroups $O_{25}$. Of 17 classic enteropathogenic E. coli(EPEC), 7(41%) were $MRHA^+$ but all were negative for CFA I(CFA $I^-$). Of 30 enteroadherent E. coli(EAEC) strains, 11(37%) were $MRHA^+$ and one was CFA $I^+$. Of 24 nonpathogenic E. coli, 4(17%) were $MRHA^+$ but all were CFA $I^-$. It was shown that MRHA was common in all strains of E. coli, CFA I was limited only to ST producing ETEC and EAEC; although MRHA is a useful screening procedure, serologic tests seem to be necessary to comfirm CFA I production. CFA I was associated with a lower proportion of ETEC isolates in Korea than has been reported for other locations.

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디지털 카메라에서 컬러 필터 어레이를 위한 평가 시스템 (Evaluation System for Color Filter Array (CFA) in Digital Camera)

  • 배태욱
    • 한국멀티미디어학회논문지
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    • 제20권11호
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    • pp.1741-1749
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    • 2017
  • In commercial digital-cameras, color-filter filters light according to wavelength range of color filter array (CFA) and the filtered intensities contain color information of light. Then, output data of CFA is transformed to final rendered image through demosaicing process. In image processing of digital-camera, the quality of the final rendered image is affected by optical cross talk of CFA, kind of CFA pattern etc. Basically, pattern of CFA plays important role in image quality of final image rendered by digital-camera. Therefore, an evaluation system capable of quantitatively evaluating CFA is needed. This paper proposes a novel evaluation system using existing and proposed image metrics for evaluating CFAs of digital-camera. Proposed CFA evaluation system consist of color difference in CIELAB and S-CIELAB, Structure SImilarity (SSIM), MTF50, moire starting point (MSP), and subjective preference (SP). MSP and SP are newly designed for the proposed evaluation system. Proposed evaluation system is expressed in polar coordinates to analyze the characteristics of CFA objectively and intuitively. Through simulations, we confirmed that proposed CFA evaluation system can objectively assess performance of developed CFAs.

Antigenicity Study of CFA-001, cefazolin, a cephalosperin Derivative Produced by an :Enzymatic Semisynthesis

  • Park, Jong-Il;Jeong, Tae-Cheon;Kim, Hyoung-Chin;Han, Sang-Seop;Roh, Jung-Koo;Kim, Jeong-Hwan;Jeon, Yeong-Joong;Kim, Dal-Hyun;Kim, Je-Hak;Park, Kwan-Ha
    • Biomolecules & Therapeutics
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    • 제5권1호
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    • pp.48-52
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    • 1997
  • The antigenic potential of CFA-001, cefazolin, a cephalosporin derivative produced by an enzy-matic semisynthesis, was determined in Hartley guinea pigs. A battery of tests employed consisted of active systemic anaphylaxis (ASA), passive cutaneous anaphylaxis (PCA), and indirect hemagglutination test (IHA). The results were as follows: 1) In ASA, no signs attributable to anaphylaxis was observed in guinea pigs sensitized with CFA-001, whereas OVA-sensitized animals induced severe anaphylactic symptoms; 2) guinea pigs did not produce antibodies against CFA-001 when sensitized with or without Freund's complete adjuvant (FCA) in homologous PCA tests. Meanwhile, antibodies against ovalbumin (OVA) were clearly detected; 3) No CFA-001-specific hemagglutination was observed in the IHA using sera obtained from CFA-001- sensitized guinea pigs. These results suggest that CFA-001 has no antigenicity potential in guinea pigs.

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Effects of Coal Fly Ash as a Bulking Agent under Co-composting with Swine Manure and Saw Dust

  • Lee, Chang Hoon;Park, Seong Jin;Kim, Myung Sook;Yun, Sun Kang;Sonn, Yeon Kyu
    • 한국토양비료학회지
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    • 제47권5호
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    • pp.356-361
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    • 2014
  • The coal fly ash (CFA) may be utilized as an extender for organic waste composting at the same time fully expected to solve all industrial waste disposal and sawdust tribe. The main objective in this study was to evaluate the effect of CFA addition as a bulking agent for swine manure composting. To determine the suitable addition rate of CFA as a bulking agent, 0, 10, 20 and 30% of saw dust were mixed with 30, 20, 10 and 0% of coal fly ash, respectively. Compost quality for swine manure composting was to evaluate temperature, pH, C/N ratio, and phytotoxicity as germination index. Stability of compost increased with increasing levels of CFA as bulking agent during swine manure composting due to the high alkaline materials including CFA. C to N ratio in treatment added CFA was higher than that of the control without CFA. After finishing composting, germination index of lettuce and cabbage in swine manure compost added 10% of CFA was similar to the control, all the heavy metal contents were far below the stipulated standard for organic farming. These results indicated tahr coal fly ash as bulking agents might be alternative materials to save saw dust and apply industrial products for swine manure composting.

유한요소법을 이용한 쿨링팬의 진동 및 간섭에 관한 연구 (A Study on Resonance and Interference of a Cooling Fan Assembly by Using FEM)

  • 정일호;송하종;박태원;김주용
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2004년도 춘계학술대회논문집
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    • pp.919-924
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    • 2004
  • A CFA(Cooling Fan Assembly) is composed of a fan, motor and shroud, which is at the back of the automotive radiator. By forcing the wind to pass, the CFA controls the cooling performance of the radiator. The noise and vibration of the CFA may be primarily due to the resonance between the CFA and engine. The Interference among the fan, shroud and radiator by deformation is considered when the CFA is designed. In this paper, in order to analyze the structural vibration of the CFA for automobiles, a finite element model of the CFA is established by using a commercial FEM code. After the finite element modeling, the natural frequencies and the mode shapes are obtained from the FE analysis. The natural frequencies are obtained from the vibration test as well. Then, the results of the vibration test are compared with those of the FE analysis. The natural frequencies obtained by experiment have a great similarity to the results from FE model. We have confirmed the validity of the FE model and verify the structural safety for the resonance. The stress and displacements are obtained from FE analysis. We have confirmed the safety for the interference and failure.

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환도(環跳)(GB30)·양릉천(陽陵泉)(GB34) 온침(溫鍼)이 CFA로 유발된 백서의 류마티스 관절염에 미치는 영향 (The Effects of Warm Needling at GB30·GB34 on Complete Freund's Adjuvant(CFA)-induced Rheumatoid Arthritis in Rats)

  • 정주용;조명래
    • Journal of Acupuncture Research
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    • 제32권2호
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    • pp.65-75
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    • 2015
  • Objectives : The purpose of this study was to investigate the effects of warm needling at $GB_{30}{\cdot}GB_{34}$ on Complete Freund's Adjuvant(CFA)-induced rheumatoid arthritis in rats. Methods : Arthritis was induced by injecting CFA subcutaneously into the left knee joint and paw. Acupuncture(AT) and warm needling(W-AT0.5, W-AT1.0) were injected at $GB_{30}{\cdot}GB_{34}$, every other day for a total of 5 times beginning on day 10 after the CFA injection. Thereafter, external shape, paw edema, serum aminotransferase and anti-inflammatory factors were assessed, and hematological and histological observations were made. Results : In paw edema volume all 3 groups(AT, W-AT0.5, W-AT1.0) showed significant decrease compared to the CFA control group. In TNF-${\alpha}$ and IL-6, all 3 groups showed significant decrease compared to CFA control group. In AST and ALT all 3 groups showed no significant change. In IL-$1{\beta}$, W-AT0.5 and W-AT1.0, groups showed significant decrease compared to the CFA control group. Leucocyte, erythrocyte and thrombocyte, all 3 groups showed no significant change. In histological observations, all 3 groups were similar to the intact group in terms of synoviocyte, cartilage lacuna and cartilage cells. Conclusions : The results suggest that warm needling at $GB_{30}{\cdot}GB_{34}$, has the effect of suppressing inflammation of CFA-induced rheumatoid arthritis in rats.

$1{\alpha},\;25\;Dihydroxyvitamin\;D_3$이 랫드 골다공증에 미치는 영향 (Effect of $1{\alpha},\;25\;Dihydroxyvitamin\;D_3$ on Osteoporosis in Rats)

  • 배춘식;최석화
    • Applied Microscopy
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    • 제32권3호
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    • pp.223-230
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    • 2002
  • Sprague-Dawley rat에 complete Freund's adjuvant (CFA)를 투여하여 유발한 골다공증에 미치는 $1{\alpha}$, 25 dihydroxyvitamin $D_3\;(Vit\;D_3)$의 효과를 알아보기 위하여 실험동물을 정상 대조군, CFA 투여 대조군, CFA 투여후 $1{\alpha}$, 25 dihydroxyvitamin $D_3\;0.01{\mu}g/kg$ 투여군 (Vit $D_{3}L$) 및 $0.1{\mu}g/kg$ 투여군(Vit $D_{3}H$)으로 분류하여 경구로 3주간 투여하였다. 골다공증의 억제 효과를 알아보기 위하여 골밀도와 골함유량의 변화 및 주사전자현미경적 소견을 관찰한 결과 다음과 같은 결론을 얻었다. 골밀도와 골함유량은 $1{\alpha}$, 25 dihydroxyvitamin $D_3$ 투여용량과 부위에 따라 차이가 있었는데 Vit $D_3$ 투여군이 CFA 투여 대조군과 비교하여 골밀도와 골함유량이 증가하였으며 Vit $D_{3}H$군이 Vit $D_{3}L$군보다 좀 더 효과적이었다. 대퇴골의 주사전자현미경적 소견은 Vit $D_3$ 투여군의 골피질과 골소주의 손상이 CFA 투여 대조군보다 억제되었음을 볼 수 있었으며 Vit $D_{3}H$ 투여군이 Vit $D_{3}L$ 투여군보다 좀 더 효과적이었다. 이상의 결과를 종합해 보면 $1{\alpha}$, 25 dihydroxyvitamin $D_3$의 투여는 CFA에 의해 유발된 rat의 골다공증 모델에서 골다공증의 진행을 억제하였고 Vit $D_{3}H$ 투여군이 Vit $D_{3}L$ 투여군보다 좀 더 효과적이었다.

A Pseudo Multiple Capture CMOS Image Sensor with RWB Color Filter Array

  • Park, Ju-Seop;Choe, Kun-Il;Cheon, Ji-Min;Han, Gun-Hee
    • JSTS:Journal of Semiconductor Technology and Science
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    • 제6권4호
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    • pp.270-274
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    • 2006
  • A color filter array (CFA) helps a single electrical image sensor to recognize color images. The Red-Green-Blue (RGB) Bayer CFA is commonly used, but the amount of the light which arrives at the photodiode is attenuated with this CFA. Red-White-Blue (RWB) CFA increases the amount of the light which arrives at photodiode by using White (W) pixels instead of Green (G) pixels. However, white pixels are saturated earlier than red and blue pixels. The pseudo multiple capture scheme and the corresponding RWB CFA were proposed to overcome the early saturation problem of W pixels. The prototype CMOS image sensor (CIS) was fabricated with $0.35-{\mu}m$ CMOS process. The proposed CIS solves the early saturation problem of W pixels and increases the dynamic range.

Effect of Electroacupuncture on AMPA Receptor GluR2 Subunit in Complete Freund's Adjuvant-induced Inflammatory Pain Model

  • Kim, Chul-Yun;Choi, Hye-Young;Yang, Yeun-Jin;Choi, Byung-Tae
    • 동의생리병리학회지
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    • 제25권5호
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    • pp.881-886
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    • 2011
  • AMPA receptor (AMPAR)s are heterotetrameric structures made up from 4 units (GluR1-4) and are thought to underlie perception of persistent inflammatory pain. Complete Freund's adjuvant (CFA)-evoked inflammation induces synaptic GluR2 internalization, which is initiated by GluR2 phosphorylation, in dorsal horn neurons during the maintenance of CFA-induced hypersensitivity. The present study investigated whether electroacupuncture (EA) stimulation has any effect on GluR2 trafficking by using immunoblot and immunohistochemistry. We examined that CFA-induced dorsal horn GluR2 internalization was attenuated by EA treatment. EA treatment could also decrease the level of pGluR2 regardless of whether CFA injection was administrated or not. In addition, previous studies suggest that microglial cells are increased without morphological change in CFA injected animal. In our study, increases in microglial cells in CFA group were observed, whereas EA with or without CFA-injected group showed similar aspects with normal group. In conclusion, our results indicate that EA might blunt CFA-evoked inflammation by coordinating mechanisms at the upstream step of neuron activation and GluR2 phosphorylation.

Hydrazine-Copper 방법을 이용한 연속흐름제어장치를 통한 식물체의 nitrate 분석 (Continuous-Flow Analysis for Determination of Nitrate Using Hydrazine-Copper Method in Plan)

  • 박양호;박소현;이주영;장병춘;이기상
    • 한국토양비료학회지
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    • 제39권4호
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    • pp.204-208
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    • 2006
  • 이연구의 목적은 연속흐름제어장치를 통한 식물체의 nitrate 분석방법 및 RQflex 방법 및 salicylic acid 방법과 비교를 통해 그 정확성을 확인하고자 함에 있다. 분석은 메론, 수박, 오이, 그리고 토마토 잎을 현장에서 채취 후, 분석시까지 초저온 냉동고에 보관후 분석하였다. RQflex로 측정한 결과가 CFA방법이나 salicylic acd 방법보다 약간 높게 나왔으며, CFA방법과 salicylic acid 방법과의 상관관계는 $R^2=0.9671$을 보였으며, RQflex와의 상관관계는 $R^2=0.9739$를 보였다.