• 동의생리병리학회지
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• 제18권4호
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• pp.1021-1027
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• 2004

Panax ginseng has been used as a typical tonic medicine in Asian countries, such as Korea, China, and Japan. It has been reported that ginsenoside Rg1 in Panax ginseng increases the proportion of T helper cells in the whole T cells and promotes IL-2 gene expression in murine splenocytes. These studies imply that ginsenoside Rg1 increases the immune activity of CD4+ T cell, however the exact mechanism of ginsenoside Rg1 on helper T cell remains to be verified. The present study tried to elucidate the direct effect of Rg1 on helper T cell s activities and its Th1/Th2 lineage development. The results demonstrated that ginsenoside Rg1 had not mitogenic effects on the unstimulated CD4+ T cell, but augmented CD4+ T cell proliferation upon activating with anti-CD3/anti-CD28 antibodies in a dose dependent manner. Rg1 also enhanced the expression of cell surface protein CD69 on CD4+ T cell. In Th0 condition, ginsenoside Rg1 increases the expression of IL-2 mRNA, and enhances the expression of IL-4 mRNA on CD4+ T cells, suggesting Rg1 prefer to induce Th2 lineage development. In addition, ginsenoside Rg1 increases IL-4 secreting CD4+ T cell under Th2 skewed condition, while decreases IFN-γ secreting cell in Th1 polarizing condition. Thus, Rg1 enhances Th2 lineage development from naive CD4+ T cell both by increasing Th2 specific cytokine secretion and by repressing Th1 specific cytokine production. Therefore, these results suggest that ginsenoside Rg1 might be desirable agent for enhancing CD4+ T cell's activity, as well as the correction of Th1 dominant pathological disorders.

• 한국환경보건학회지
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• 제18권2호
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• pp.135-145
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• 1992

This study was carried out to find out some characteristic responses of biological samples under the exposure of trace metals with the considering of Sex, age, residence period and smoking. Blood, Urine and Hair were collected from 116 dwellers in the vicinity of a smelting factory. Cd, Cu, Pb and Zn concentrations were determined by flame atomic absorption spectrophotometry and the following results were found important. 1) The concentrations of trace metals in the blood were 0.010+ 0.003 $\mu$g/ml in Cd, 0.82+ 0.11 $\mu$g/ml in Cu, 0.23_+ 0.13 $\mu$g/ml in Pb and 5.78_+ 1.43 $\mu$g/ml in Zn, those in the urine were 1.7_+ 1.7 $\mu$g/l in Cd, 16.5_+ 16.0 $\mu$g/l in Cu, 24.6_+ 23.0 $\mu$g/l in Pb and 367.7_+ 283.4 $\mu$g/l in Zn, and those in the hair were 0.52_+ 0.56 $\mu$g/g in Cd, 11.00_+4.01 $\mu$g/g in Cu, 8.53_+ 7.05 $\mu$g/g in Pb and 383.23_+ 110.56 $\mu$g/g in Zn. 2) In sex, the concentrations of Zn in the blood and urine of male were higher than those of female, however, the concentrations of Cd and Zn in the hair of female were higher than those of male. 3) The concentrations of Cd, Pb and Zn in the urine showed an increasing trend by age. 4) While the concentration of Cd in the urine increased, the concentration of Pb in the hair decreased by residential period in all the samples. 5) The concentrations of Cd and Zn in the blood and that of Zn in the urine of smoking group were higher than those of non-smoking group. The concentration of Pb in the blood of smoking group and those of Cd, Pb and Zn in the urine of non-smoking group showed increasing the trend by age. The concentrations of Cd and Zn in the urine of non-smoking group, the concentration of Zn in the hair of smoking group showed increasing trend by residential period, however, the concentrations of Pb in the blood and hair and that of Zn in the hair showed decreasing trend by residential period. 6) When the correlations of the concentrations between Zn (main smelting metal) and other metals were analyzed, Zn in the blood was significantly correlated with Cu, Pb and Zn in the urine (positively) and Zn in the urine was significantly correlated with Zn in the blood, Cd, Cu and Pb in the urine, and Pb in the hair (positively). Zn in the hair was significantly correlated with Cd in the urine and Cu in the hair (positively). 7) Consequently, it was useful to note that samples of urine showed higher sensitivity under the metal exposure than those of blood and hair in individual sampling. In addition, as trace metals showed higher accumulation in the hair of the groups, sampling of hair was more highly recommended than those of urine and blood in group sampling.

• 한국환경농학회지
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• 제20권3호
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• pp.175-179
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• 2001

우리나라 주요 밭작물의 생육에 미치는 카드뮴의 영향을 구명하기 위하여 토양 중 카드뮴의 농도를 무처리, 5, 10, 25, 50, 100 mg/kg을 처리한 후, 배추, 무, 상추를 재배하여 작물의 생육피해양상과 작물체내 카드뮴의 흡수정도를 조사하였다. 카드뮴에 의한 생육피해 증상은 배추, 상추에서 발아 후 생육초기 생육저해와 잎 가장자리부터의 황백화 현상이 관찰되었다. 5%의 유의적인 생육 및 수량의 감소를 초래하는 생육 피해농도는 배추가 $50{\sim}100$ mg/kg, 상추는 $10{\sim}25$ mg/kg이었으나, 무는 100 mg/kg일 때에서도 생육저해를 받지 않아 작물에 따른 피해 농도가 다름을 알 수 있었다. 토양중 카드뮴 농도가 증가함에 따라 작물 가식부위별 생체중 카드뮴 함량은 상추는 5 mg/kg 배추와 무는 25 mg/kg부터 유의적으로 증가하였으며, 가식 부위의 카드뮴 함량은 토양 중 카드뮴 농도가 5 mg/kg일 때 상추, 배추, 무 각각 3.37, 0.13, 0.08 mg/kg F.W.이었다. 개체당 총 흡수량은 25 mg/kg이상의 고농도 카드뮴 토양에서는 배추 > 무 > 상추순으로 증가하였으나 10 mg/kg 아래의 저농도에서는 상추의 흡수량이 배추나 무보다 높았다.

• Journal of Ginseng Research
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• 제40권1호
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• pp.18-27
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• 2016

Background: It is not clear whether ginseng affects cyclosporine A (CsA)-induced desirable immunosuppressive action. In this study, we evaluated the immunological influence of combined treatment of ginseng with CsA. Methods: Using CD4+ T cells from mouse spleens stimulated with the T cell receptor (TCR) or allogeneic antigen-presenting cells (APCs), we examined the differentiation of naïve T cells into T helper 1 (Th1), Th2, Th17, and regulatory T cells (Tregs), and their cytokine production during treatment by Korean Red Ginseng extract (KRGE) and/or CsA. The influence of KRGE on the allogeneic T cell response was evaluated by mixed lymphocyte reaction (MLR). We also evaluated whether signal transducer and activator of transcription 3 (STAT3) and STAT5 are implicated in this regulation. Results: Under TCR stimulation, KRGE treatment did not affect the population of CD4+interferon gamma ($IFN{\gamma}$)+ and CD4+interleukin (IL)-4+ cells and their cytokine production compared with CsA alone. Under the Th17-polarizing condition, KRGE significantly reduced the number of CD4+IL-17+ cells and CD4+/phosphorylated STAT3 (p-STAT3)+ cells, but increased the number of CD4+CD25+forkhead box P3 (Foxp3)+ cells and CD4+/p-STAT5+ cells compared with CsA alone. In allogeneic APCs-stimulated CD4+ T cells, KRGE significantly decreased total allogeneic T cell proliferation. Consistent with the effects of TCR stimulation, KRGE reduced the number of CD4+IL-17+ cells and increased the number of CD4+CD25+Foxp3+ cells under the Th17-polarizing condition. Conclusion: KRGE has immunological benefits through the reciprocal regulation of Th17 and Treg cells during CsA-induced immunosuppression.

• 혜화의학회지
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• 제18권1호
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• pp.29-41
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• 2009

Wished to examine closely effect that SoPungDoJeokTang-KaMi (SPDJTK) medicines used to atopy dermatitis disease patient get in atopy eruption control experimentally. SPDJTK medicines controlled $CD4^+/IFN-\gamma$, and $CD4^+/CD25^+/foxp3^+$ revelation that an experiment that motive allergy immune reponse because an in vitro experiment stimulates T cells of a NC/Nga mouse same time by anti-CD40/rmIL-4, and interleukin-$1{\beta}$, IL-6, TNF-$\alpha$, and TGF-$\beta$ mRNA outturn that bear in T and B cells decreased remarkably by SPDJTK medicines. Intracellular staining of splenocytes anti-CD40/rmIL-4 plus rmIL-4 stimulated as described in a, assessed after 24 h, SPDJTK exerts a mainly immunosuppressive effect that acts at least partially through suppression of the transcription factor GATA3 expression in $CD4^+$ T cells. Atopic dermatitis (AD) usually develops in patients with an individual or family history of allergic diseases, and is characterized by chronic relapsing inflammation seen specially in childhood, association with IgE hyperproduction and precipitation by environmental factors. However, the exact etiology of AD has been unclear. To further explore the pathogenesis and treatment of AD, a suitable animal model is required. We found that skin lesions, which were clinically and histologically very similar to human AD, mite antigen-induced dermatitis on the face, neck, ears and dorsal skin of inbred NC/Nga mice. Result that Th1 cell and Th2 cell observe to be shifted by cytokine expression with $CD4^+/CD25^+/foxp3^+$ Treg cells induction by SPDJTK medicines could know that SPDJTK medicines can use usefully in allergy autoimmnune diease.

• 대한예방한의학회지
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• 제5권1호
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• pp.76-89
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• 2001

OMH which is known for its properties of recruiting vitality, is composed of Polygonum multiflorum Thunb. and Sesamum indicum DC.. This formula is known to possess the properties of recruiting vitality, blackening white hair and expanding life span. 16-week-old SD-Rats were treated with OMH for 16 days. After 24 hours, the rats were treated with MTX(Mthotrexate is oral administrated for 4 days(1mg/kg/day), in order to lower immunity. Then, These rats were classified in to groups, the N-16 group(not specially tested), the Control group(MTX), the OMH-L group(2.5% OMH+MTX)&the OMH-H group(10% OMH+MTX) and 6 rats were assigned to each group. After 18 hours from MTX treatment the organ index of the rats from each group Thymus and Spleen were measured. The percentage of CD+4, CD8+ T cell were measured and compared by flow cytometer. 1) Rats from the OHM-L&OMH-H group showed higher organ indexes of the Thymus and Spleen compared to the rats from the Control Group. This proves that OMH possesses the properties to mitigate degenerations of immunity($F_{thymus}=20.162,\;F_{spleen}=5.882$, ANDVA, p<0.05). 2) The rats from the two OMH groups showed higher rates of CD4+ T cell counts compared to the control group(F=26.906, ANOVA, p<0.05). CD8+ T cell showed lower rates compared to the Control group, but showed no differences within the two OMH groups(F=1.254, ANOYA, p>0.05). CD4+/CD8+ showed higher rates in the two OMH groups compared to the control group which can be thought as a proof that OMH prevents depression of immune response(F=10.554, ANOVA, p<0.05). In this test 16 week-old rats were used, which can be considered as the middle and prime age of the human being. These rats were treated with OMH which ended up showing properties of mitigating degeneration of immune responses and maintaining T-cell rates within the blood. It was possible to study that OMH possesses the properties to increase immune responses.

• 정보처리학회논문지C
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• 제18C권2호
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• pp.71-78
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• 2011

현금자동입출금기/현금지급기에서 비밀번호를 입력할 때 신용카드나 통장의 비밀번호가 노출되어 자신도 모르게 현금이 인출되는 금융사고가 빈번하게 발생하고 있으므로 비밀번호를 입력하는 과정에서 각별한 주의가 요청되고 있다. 본 논문은 현금자동입출금기/현금지급기를 사용할 때 어깨너머로 비밀번호를 훔쳐보는 것을 방지하기 위하여 비밀번호를 안전하게 입력하는 방법을 제안하였다. 제안한 방법은 사용자가 비밀번호를 입력하는 과정을 옆이나 뒤에서 훔쳐봐도 무엇을 입력하였는지를 알 수 없도록 숫자들이 무작위 순으로 표시되었다가 사라진 상태에서 비밀번호를 입력함으로써 비밀번호 훔쳐보기를 원천적으로 차단하여 비밀번호가 유출되는 것을 방지할 수 있다. 비밀번호를 안전하게 입력하는 방법은 훔쳐보기 시험, 직관적인 관점 및 이론적인 분석으로 구분하여 안전성을 검증하였다. 또한, 현금자동입출금기/현금지급기에서 적용할 수 있도록 구현한 결과는 비밀번호 훔쳐보기 공격으로부터 비밀번호 획득 확률이 기존 방법보다 현저하게 낮은 것으로 평가되어 비밀번호가 유출되는 것을 방지할 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.869-874
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• 2015

Background: The prognostic value of microvessel density (MVD), reflecting angiogenesis, detected in ovarian cancer is currently controversial. Here we performed a meta-analysis of all relevant eligible studies. Materials and Methods: A comprehensive search of online PubMed, Medline, EMBASE and Sciencedirect was performed to identify all related articles. The search strategy was designed as 'microvessel density', 'ovarian cancer', 'ovarian neoplasm', 'CD34' and 'angiogenesis'. Results: The studies were categorized by author/year, number of patients, FIGO stage, histology, cutoff value for microvessel density, types of survival analysis, methods of hazard rations (HR) estimation, HR and its 95% confidence interval (CI). Combined hazard ratios suggested that high MVD was associated with poor overall survival (OS) and progression-free survival (PFS), with HR and 95% CIs of 1.84 (1.33-2.35) and 1.36 (1.06-1.66), respectively. Subgroup analysis showed that high MVD detected by CD34 was relevant for OS [HR=1.67 (1.36-2.35)], but not MVD detected with other antibodies [HR=2.11 (0.90-3.31)]. Another subgroup analysis indicated that high MVD in patients without pre-chemotherapy, but not with pre-chemotherapy, was associated with OS [HR=1.88(1.59-2.18 and HR=1.70 (-0.18-3.59)]. Conclusions: The OS and PFS with high MVD were significant poorer than with low MVD in ovarian cancer patients. However, high MVD detected by CD34 seems to be more associated with survival for patients without pre-chemotherapy.

• 한국광학회지
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• 제18권1호
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• pp.74-78
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• 2007

본 논문에서는 포토마스크 후면에 위상 패턴을 형성하여 웨이퍼 상 CD(critical dimension) 균일도를 개선할 수 있는 투과율 조절 포토마스크 기술을 ArF 리소그래피에 적용한 결과에 대하여 보고한다. 위상 패턴 조밀도에 따른 노광 광세기 변화 계산에 포토마스크 후면으로부터 포토마스크 전면까지의 광의 전파를 고려하여 ArF 파장에서의 위상 패턴 조밀도에 따른 노광 광세기 저하에 관한 실험결과를 이론적으로 재현할 수 있었다. 본 기술을 ArF 리소그래피에 적용하여 DRAM(Dynamic Random Access Memory)의 한 주요 레이어에 대해 필드 내 CD 균일도를 $3{\sigma}$ 값으로 13.8 nm에서 9.7 nm로 개선하였다.

• 대한불안의학회지
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• 제18권2호
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• pp.48-55
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• 2022

Objective : Childhood trauma is a risk factor for and resilience is a protective factor against later affective symptoms. The current study aimed to explore the relation between childhood trauma and anxiety symptoms and the mediating effect of resilience in a sample of college students. Methods : Data from 238 subjects who completed the Beck Anxiety Inventory (BAI), the Childhood Trauma Questionnaire (CTQ) and the Connor-Davidson Resilience Scale (CD-RISC) were analyzed. Predictors for BAI score and factor analyzed anxiety symptom dimensions were examined using regression models. Path analytic models were applied to test the mediating effect of the CD-RICS score on BAI score. Results : The CTQ score was significantly associated with the BAI score and the mediating effect of CD-RISC was significant as well. The cognitive dimension of anxiety was related to emotional abuse while the somatic dimension of anxiety to physical neglect. The CD-RISC score did not mediate those relations between anxiety dimensions and individual types of childhood trauma. Conclusion : Our data suggest that childhood trauma contributes to adult anxiety symptoms. There may be specific relations between types of childhood trauma and anxiety symptoms dimensions.