• Korean Journal of Optics and Photonics
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• v.8 no.4
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• pp.255-259
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• 1997

In this paper, the result of a performance study on a home-built spinning disk type confocal microscope is presented. The confocal microscope was fabricated with a Nipkow disk made of the made of the microfilm. The throughput of the disk was 0.5%, allowing the observation of specimen with higher reflectivities only. A laser diode at 692.7nm was used as the light source. The topographic structures of a PC ROM and the CD ROM were observed with sufficient reliability, while the effect of the convolution of the beam size with the finite object size was found dominant. Also the shadowing effect by the etched pattern was observed.

• Journal of the East Asian Society of Dietary Life
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• v.9 no.3
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• pp.363-369
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• 1999

The heavy metal removability of four kinds of tea was investigated in the various conditions: particle size of tea(10, 35. 70, 100 mesh), concentration of heavy metal(25, 50, 100ppm) and extraction temperature(30. 50, 70. 10$0^{\circ}C$). The removabilities by the tea solids were increased as the particle size decreased, concentration of heavy metal increased, and extracting temperature increased. Of the four kinds of tea Pb, Cd and Cu removability by Eucommia ulmoides Oliv tea was the highest. In addition, heavy metal removability by Schizandra chinensis Baillon tea was better than that of other tea as extraction temperature was increased.

• Journal of Wetlands Research
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• v.13 no.3
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• pp.523-534
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• 2011

In this study, the particle size distribution and pollutants content of sediments collected from different sites in the coastal area of Chungnam province were analyzed. The sediment samples were collected from different parking lots near beaches, harbors, museum and bridges. The particle size distribution analysis showed that the particle is generally $106-500{\mu}m$ and the effective size $D_{10}$ ranges from 40 to $60{\mu}m$ while the $D_{50}$ and $D_{60}$ ranges from 200 to $810{\mu}m$ and 235 to $1005{\mu}m$, respectively. For particle size $D_{10}$, there was no significant difference in all sites. However, for $D_{50}$ and $D_{60}$, the range is large. Sediment analysis implicates that as the particle size decreases, the pollutant content increases. This is because smaller particles have higher specific surface area resulting to have more adsorption capacity. Particles from tires, emission gas from vehicles and dust particles belongs to smaller particles. For particle sizes less than $63{\mu}m$, the analysis showed that as the particles become coarser, the concentration of VS, $COD_{cr}$, TN, and TP is at least 2 to 14 times higher. Cu and Pb were detected in all sites and shows a higher concentration with smaller particle size. Cu concentration are almost the same for all sites but in the case of Pb, the sediments from Sinjindo has higher concentration of up to 2 to 3 times as compared to those collected from the other sites. In the Beach site as well as in the Seocheon Ocean Museum, Cd was contained only in fine particles. However, in Daechon Harbor and Sinjindo Bridge sites, Cd was detected in all the sediment particle size.

• Analytical Science and Technology
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• v.12 no.1
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• pp.53-60
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• 1999

Dissolution properties for the 6 inorganic pollutants (As, Cd, Cu, Pb, Zn, Cr) in soils have been studied. These 6 inorganic pollutants were spiked to 3 kinds of fresh soils which were sand, clay, and loam. The dissolution properties of the prepared samples were investigated under the various extracting conditions such as extracting time, acid concentration, particle size, etc. in order to obtain basic information about the process of extraction test and improvement of related analytical methods. As the results, dissolution properties were affected mainly by acid concentration in extracting procedure and mineral composition of soils. On the other hand, extracting time, sort of acids and particle size of soils had a little influence on the dissolution properties. Cd revealed very high dissolving efficiency and As was very low in whole extracting test.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.11a
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• pp.456-456
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• 2008

In the past few years, the deposition and characterization of cadmium sulfide semiconducting thin films has received a considerable amount of interest due to their potential application in the area of electronic and opto-electronic devices fabrications. Polycrystalline CdS thin films posses good optical transmittance, wide band-gap and electrical properties makes it as one of the ideal material for their application to solar cell fabrication. Cadmium sulfate thin films were deposited by the chemical bath deposition method using tartaric acid and triethanolamine as a complexing agent. Deposition parameters such as pH, temperature, deposition time and concentration of the reactant species were optimized so as to obtain reflecting, good adherent uniform thin films on the glass substrate. Reaction mechanism of the thin film formation is also reported. The crystallographic structure and the crystallite size were studied by the X-ray diffraction pattern. The optical band-gap of deposited film is identified by measuring the transmittance in the visible region. Temperature dependence of resistivity confirmed the semiconducting behavior of the film. Scanning electron micrographs (SEM) showed the presence of grain particles of size 50 nm.

• Journal of Acupuncture Research
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• v.24 no.6
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• pp.45-61
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• 2007

Objectives : The purpose of this study is to observe the effects of Carthami Flos herbal-acupuncture (CF-HA) at Joksamni($ST_{36}$) on arthritis in mice induced by Collagen II. Methods : The author performed several experimental items, including arthritis evaluation, change in weight, spleen size and stenosis rate, change in cytokine level, IgG, IgM and anti-collagen II, change of immunocyte count and histological change of the CIA mouse joint. Conclusions are as follows: Results : 1. In the CF-HA, the arthritis index and rate and the incidence of arthritis were decreased as the experiment proceeded. 2. In the CF-HA, spleen swell and stenosis, joint edema and change were decreased. 3. In the CF-HA, the level of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ and $IFN-{\gamma}$ in blood serum were significantly decreased. 4. In the CF-HA, the level of IgG, IgM and anti-collagen II were decreased. 5. In the CF- HA, $IFN-{\gamma}$, $IFN-{\gamma}/IL-4$, IL-10 of the culture fluid was decreased. 6. In the CF-HA, the cell rate of $CD3e^+$ and $CD45R^+$, $CD4^+$ and $CD8^+$, $CD4^+/CD25^+$ in spleen was similar to the cell rate of the normal group. 7. In the CF-HA, the cell rate of $CD4^+/CD25^+$, $CD45R^+/CD69^+$ in a lymph node was decreased as in the normal group. 8. In the CF-HA, the cell rate of $CD3^+/CD69^+$, $CD11b^+/Gr-1^+$ in joints was decreased as in the normal group. 9. In the CF-HA, the cartilage destruction and the inflammation cell growth in the H&E stain were decreased. The collagen fiber in the M&T stain were less destructed, therefore the result was similar to the normal group. Conclusions : These results suggest that CH-HA at $ST_{36}$ has an effect in controlling immune reaction and suppressing inflammation in rheumatoid arthritis therefore, the continuous flow of the following study is expected.

• Laser Solutions
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• v.7 no.3
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• pp.37-46
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• 2004

The shape and size variations of the nanopatterns produced on a polymer film using a near-field scanning optical microscope(NSOM) are investigated with respect to the process variables. A cantilever type nanoprobe having a 100nm aperture at the apex of the pyramidal tip is used with the NSOM and a He-Cd laser at a wavelength of 442nm as the illumination source. Patterning characteristics are examined for different laser beam power at the entrance side of the aperture($P_{in}$), scan speed of the piezo stage(V), repeated scanning over the same pattern, and operation modes of the NSOM(DC and AC modes). The pattern size remained almost the same for equal linear energy density. Pattern size decreased for lower laser beam power and greater scan speed, leading to a minimum pattern width of around 50nm at $P_{in}=1.2{\mu}W\;and\;V=12{\mu}m/s$. Direct writing of an arbitrary pattern with a line width of about 150nm was demonstrated to verify the feasibility of this technique for nanomask fabrication. Application on high-density data storage is discussed.

• IMMUNE NETWORK
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• v.9 no.4
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• pp.138-146
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• 2009

Background: The MHC region of the chromosome contains a lot of genes involved in immune responses. Here we have investigated the mouse NG29/Cd320 gene in the centrometrically extended MHC region of chromosome 17. Methods: We cloned the NG29 gene by RT-PCR and confirmed the tissue distribution of its gene expression by northern blot hybridization. We generated the NG29 gene expression constructs and polyclonal antibody against the NG29 protein to perform the immunofluorescence, immunoprecipitation and flow cytometric analysis. Results: The murine NG29 gene and its human homologue, the CD320/8D6 gene, were similar in the gene structure and tissue expression patterns. We cloned the NG29 gene and confirmed its expression in plasma membrane and intracellular compartments by transfecting its expresssion constructs into HEK 293T cells. The immunoprecipitation studies with rabbit polyclonal antibody raised against the NG29-NusA fusion protein indicated that NG29 protein was a glycoprotein of about 45 kDa size. A flow cytometric analysis also showed the NG29 expression on the surface of Raw 264.7 macrophage cell line. Conclusion: These findings suggested that NG29 gene in mouse extended MHC class II region was the orthologue of human CD320 gene even though human CD320/8D6 gene was located in non-MHC region, chromosome 19p13.

• Analytical Science and Technology
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• v.12 no.2
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• pp.105-110
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• 1999

The studies on the adsorption properties and the antibacterial effects of the Cd and Cu-treated activated carbon were carried out. From the adsorption studies on the series of these metal-treated activated carbons, typical Type-I isotherm was observed. The surface areas of the treated carbon obtained from BET equation were in the range of $1101-1418m^2/g$ for Cd-AC and of $1084-1361m^2/g$ for Cu-AC. Using ${\alpha}_s$-plot, the micropore volumes and pore size distribution were obtained. From the SEM study, it is also observed that many of micropores in activated carbon are blocked by window blocking effect of metals after the impregnation. Finally, antibacterial effects of M-activated carbon against Escherichia coli was discussed. From the study, the area of antibacterial activity becomes larger with the increase of the amount of metal treated.

• The Journal of Dong Guk Oriental Medicine
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• v.5
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• pp.197-207
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• 1996

As a mood-altering drug, long-term alcohol consumption have significant harmful effects on the human body and people's mental functioning. This study observed that the suppression of cell mediated immunity induced in spleen of ICR mouse by long-term alcohol administration. After 8% alcohol voluntary administered for 120 days, the splenic tissue irnmunohistochemically stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly-2(CD8), IL-2 receptor(CD25R) and NK-1.1(CD56) after embedding with paraffin. The results were as follows. 1. The size of marginal zone in splenic white pulp was diminished and the number of macrophage in marginal zone was decreased in test group than control group. 2. After alcohol administration, the number of Helper T lymphocyte, cytotoxic T lymphocyte, and IL-2 receptor were decreased in periarterial lymphatic sheaths of white pulp and penicilla artery of red pulp and the degree of CD4, CD8, and CD25R positive reaction were soften. 3. In test group, the number of NK cell were decreased. These results indicated that the secretion of lymphokine as IL-2 was inhibited by long-term alcohol administration and subsequently prevent to activate and proliferate splenic T lymphocytes and NK cells as cell mediated immunity component.