• Korean Journal of Oriental Medicine
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• v.15 no.3
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• pp.83-91
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• 2009

$\beta$-glucan is a fiber-type complex sugar (polysaccharide) derived from the cell wall of baker's yeast, oat and barley fiber, and many medicinal mushrooms, such as maitake. The primary uses of $\beta$-glucan are to enhance the immune system, to lower blood cholesterol levels and to treat tumor. $\beta$-glucan has no systemic toxicity in mice, therefore it needed clinical trail to prove efficacy and safety for human. The subjects total 56 healty volunteers were divided into two groups including taken $\beta$-glucan tablet group and placebo group. Subjects were taken two tablets per oral for 4 weeks. They had agreed to take part in this experiment, and didn't take any other clinical trail products. After 4 weeks blood of subjects were checked. The check list are TNF-$\alpha$, INF-$\gamma$, IL-2, IL-4, total WBC, differential WBC, RBC, hemoglobin, platelet, MCV, MCH, MCHC, HCT, Na, K, Ca, Cl, AST, ALT, ALP, $\gamma$-GTP, total protein, triglyceride, total cholesterol, total bilirubin, albumin, uric acid, creatinine, BUN, pH, protein, glucose, ketone body, blood, bilirubin. We evaluated efficacy by cytokines that compare before and after taking. Collected data were analyzed as two sample t-test, chi-square test and ANOVA using SAS V.9.1.This study results are that in TNF-$\alpha$ of $1^{st}$ efficacy measurement item, all of two groups figure were increased significantly compare to before figure. In IL4 of $2^{nd}$ efficacy measurement item, experimental group figure were decreased significantly but placebo group figure were increased. The conclusions show that based on the above results, $\beta$-glucan has favorable effect to enhance immune system, especially IL4 results showed that it has effect to improve the allergic immune system.

• Journal of Chest Surgery
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• v.44 no.1
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• pp.9-17
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• 2011

Background: A peripheral extracorporeal membrane oxygenator (p-ECMO) has been developed to support patients who are dying due to a serious cardiopulmonary condition. This analysis was planned to define the clinical situation in which the patient benefits most from a p-ECMO. Material and Methods: Between June 2007 and Aug 2009, a total of 41 adult patients used the p-ECMO. There were 23 males and 18 females (mean age $54.4{\pm}15.1$ years). All patients had very unstable vital signs with hypoxia and complex cardiac problems. We divided the patients into 4 groups. In the first group, a p-ECMO was used as a bridge to cardiac operation. In the second group, patients did not have the opportunity to undergo any cardiac procedures; nevertheless, they were treated with a p-ECMO. In the third group, patients mostly had difficulty in weaning from CPB (cardiopulmonary bypass) after cardiac operation. The fourth group suffered from many complications, such as pneumonia, bleeding, infections, and LV dysfunction with underlying cardiac problems. All cannulations were performed by the Seldinger technique or cutting down the femoral vessel. A long venous cannula of $DLP^{(R)}$ (Medtronic Inc, Minneapolis, MN) or $RMI^{(R)}$ (Edwards Lifesciences LLC, Irvine, CA) was used together with a 17~21 Fr arterial cannula and a 21 Fr venous cannula. As a bypass pump, a Capiox emergency bypass system ($EBS^{(R)}$; Terumo, Tokyo, Japan) was used. We attempted to maintain a flow rate of $2.4{\sim}3.0\;L/min/m^2$ and an activated clotting time (ACT) of around 180 seconds. Results: Nine patients survived by the use of the p-ECMO. Ten patients were weaned from a p-ECMO but they did not survive, and the remainder had no chance to be weaned from the p-ECMO. The best clinical situation to apply the p-ECMO was to use it as a bridge to cardiac operation and for weaning from CPB after cardiac operation. Conclusion: Various clinical results were derived by p-ECMO according to the clinical situation. For the best results, early adoption of the p-ECMO for anatomical correction appears important.

• Journal of Animal Environmental Science
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• v.15 no.2
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• pp.155-160
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• 2009

This study was carried out to investigate the feasibility of Multi Process of Aerobic Digestion (MPAD) for recycling of swine manure slurry as fertilizer. MPAD consisted of three kinds of difference process which are thermophilic aerobic oxidation (TAO) system, lime solidification system, and reverse osmosis (R/O) membrane system. TAO system was studied well previously for decade. The chemical composition of the lime-treated solid fertilizer was as like that organic matter 17.4%, moisture 34.1%, N 0.9%, P 1.7%, K 0.3%, Ca 12.7%, and which was expected to be useful as acid soil amendment material. The concentrated liquid material produced by R/O membrane system was also expected as a good fertilizer for crops production and soil fertility improvement.

• Journal of Animal Environmental Science
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• v.15 no.2
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• pp.161-170
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• 2009

This experiment was conducted to investigate the effects of compost leachate and concentrated pig slurry on growth of pepper in substrate hyrdoponic culture. In process of composting, compost leachate was was through a saturated compost heap. Pig slurry was filtered by ultra filtration and concentrated by reverse osmosis process. The pig slurry was mixed with chemical nutrient solution and byproduct based on nitrogen content. Peppers were grown in the seven different hydroponic solutions; compost leachate (CL), concentrated pig slurry (CS), compost leachate + byproduct (CL+BP), concentrated pig slurry + byproduct (CS+BP), compost leachate 50% + nutrient solution 50% (CL+NS), concentrated pig slurry 50% + nutrient solution 50% (CS+NS) and chemical nutrient solution for pepper. The chemical nutrient solution was the standard solution of National Horticulture Research Station for the growth of pepper. The concentration of nutrient solution was adjusted $1.6{\sim}2.0mS/cm$ in EC. The compost leachate and concentrated pig slurry were low in phosphorus (P), calcium (Ca), magnesium (Mg), but rich in potassium (K). Growth characteristics as affected by the different nutrient solution were significantly different. Growth and fruit characteristics treated with CL 50+NS 50%CS and 50+NS 50% were similar with NS 100% control plot. The dry weight of stem and leaf were 107.4, 104.2g in plot of NS 100% and CS 50%+NS 50%, respectively. The fruit of pepper showed lowest in the plot treated with 100% concentrated pig slurry, and the growth of pepper severely decreased after application of 100% CS treatment. The yield of pepper was not significantly different between the plots treated with mixture of CS50 + NS50% and 100% nutrient solution treatment. Fruit yield of the compost leachate concentrated pig slurry plot were 59, 14% compared to control, repectively. In conclusion, the mixture solution of 50% of pig slurry and 50% of nutrient solution could be used as a nutrition solution of pepper in hydroponic culture.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.1
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• pp.9-15
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• 1998

A survey was conducted to investigate the chemical properties of soils such as pH, electrical conductivity, total organic matter content, soluble nitrate, available phosphate and major exchangeable canons, in plastic film houses at 513 sites. All the parameters surveyed in the plastic film house were much higher than those of open field soils. Particularly conspicuous was the accumulation of available P, exchangeable K and the occurrence of nitrate at relatively high concentration in both top soil(0-20 cm) and sub-soil(20-40 cm). In 70-80% of cases, the contents of available P and exchangeable K in top soils, were found to be higher than optimum levels. There was positive linear correlation between the content of exchangeable rations, and nitrate and EC of soils. The correlation coefficient was greater in the order of nitrate-EC > Mg-nitrate > K-nitrate > Ca-nitrate. The successive cultivation of horticultural crops in the plastic houses tended result in the accumulation of available P, exchangeable K and total organic matter in the soil.

• The Korean Journal of Food And Nutrition
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• v.34 no.5
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• pp.477-486
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• 2021

To utilize Malus pruniforia Borkh. as a functional material, cold-water (CW), hot-water (HW), and 70% ethanol (EtOH) extracts were prepared, and their antioxidant and anti-inflammatory activities were compared. The antioxidant activity of the HW extract evaluated by ABTS and DPPH radical scavenging and FRAP activity was significantly effective. The total polyphenol content of the HW extract was also higher by 15.5±0.7 mg GAE/g extract compared to other extracts. The EtOH extract showed significantly decreased TNF-α (39.8%), IL-6 (65.5%), and NO (34.9%) levels in RAW 264.7 cells compared to the LPS-induced control group. The levels of IL-6 (21.1%) and IL-8 (19.3%) were significantly decreased by treatment of EtOH extract in HaCaT keratinocytes induced with TNF-α and IFN-γ. The UHPLC-MS results indicated that the EtOH extract might have chlorogenic acid and phlorizin as the major compounds. This was validated using HPLC-DAD, which showed that the EtOH extract had higher levels of chlorogenic acid and phlorizin (1,185±58 and 470±10 ㎍/g extract, respectively). In conclusion, the present study suggested that the anti-inflammatory activity of the EtOH extract was more effective than the CW and HW extracts, and chlorogenic acid and phlorizin could be used as indicator compounds and functional substances.

• Journal of Korean Society of Forest Science
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• v.81 no.3
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• pp.273-279
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• 1992

Protoplasts isolated from leaf mesophyll tissues of Populus koreana ${\times}$ P. nigra var. italica were fused with those of P. euramericana cv. Guardi. Well expended healthy leaves of 5 to 7 week-old-plantlet grown in vitro were used as source materials. Leaves from P. koreana ${\times}$ P. nigra var. italica and P. euramericana cv. Guardi were digested in enzyme solution I (2.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v) and enzyme solution II (1.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v), respectively, The highest frequency of fusion among the protoplasts originated from the two source materials was approximately 21% using 40% PEG or 15% dextran. In addition, fusion frequency was enhanced by incorporating 30mM of $Ca^{2+}$ in eluting solution at pH 10.5. Dividing cells and/or mint-calli were obtained by culturing the fusion products in a liquid 8p-KM medium supplemented with 0.6M sucrose, $0.45{\mu}M$ 2, 4-D, and $0.5{\mu}M$ BA. Shoots were regenerated from the fusion product-derived calli after culture on MS medium containing $5.0{\mu}M$ zeatin. To verify the putative hybrid or cybrid, SDS-PAGE was carried out. From the 24 regenerants, just two plants showed intermediate protein band patterns compared with those of the original source plants.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.124-125
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• 2002

Two experiments were conducted to investigate the response of chitosan supplementation in diet on the major economic traits of broiler in two different breeds. In the both experiments, the Arbor Acres and Ross breeds were used as experimental stocks and two groups were assigned in each breed. The control group birds(CON) were fed with basal diet only and the experimental group birds(EXP) were fed with basal diet added with 10.5mg chitosan/bird/day. The chitosan was supplied to birds from day-old in experiment 1 and from 15-day-old in experiment 2. In experiment 1, the mean body weight at 35-day-old were significantly(P〈0.05) heavier by 121.2 g and 243.7 g in the EXP groups than in the CON groups of Arbor Acres and Ross, respectively. Whereas, the mean body weights at 35-day-old in experiment 2 were lighter by 91.7 g and 70.2 g in the EXP groups than in the CON groups of Arbor Acres and Ross, respectively : however, the comparisons between breeds in the mean body weight at 35-day-old did not show significant difference in each other in both breeds. In the mean feed conversion ratio of Arbor Acres from 14 to 35-day old in experiment 1, it did not show significant difference between EXP and CON groups although the feed conversion ratio of the EXP group of Ross was significantly higher(P〈0.05) than the CON group. In experiment 2, the feed conversion ratios from 14 to 35-day-old did not show significant differences between the two breeds. The percentage of mean abdominal fat depositions of EXP groups in both breeds In experiment 1 were significantly(P〈0.05) higher than those of CON groups. And the percentage of mean abdominal fat deposition of Ross was significantly(P〈0.05) lower than that of Arbor Acres. In experiment 2, the percentage of mean abdominal fat depositions did not show significant difference between EXP and CON groups in both breeds. Whereas, the interaction effects between breed and experimental groups on the above economic traits did not show significant in both experiments.

• Radiation Oncology Journal
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• v.15 no.3
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• pp.197-206
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• 1997

Purpose : Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid (PA) and choline. Recently, PLD has been drawing much attentions and considered to be associated with cancer Process since it is involved in cellular signal transduction. In this experiment, oleate-PLD activities were measured in various tissues of the living rats after whole body irradiation. Materials and Methods : The reaction mixture for the PLD assay contained $0.1\;\muCi\;1,2-di[1-^{14}C]palmitoyl$ phosphatidylcholine 0.5mM phosphatidylcholine, 5mM sodium oleate, $0.2\%$ taurodeoxycholate, 50mM HEPES buffer(pH 6.5), 10mM $CaCl_2$, and 25mM KF. phosphatidic acid, the reaction product, was separated by TLC and its radioactivity was measured with a scintillation counter. The whole body irradiation was given to the female Wistar rats via Cobalt 60 Teletherapy with field size of 10cmx loom and an exposure of 2.7Gy per minute to the total doses of 10Gy and 25Gy. Results : Among the tissues examined, PLD activity in lung was the highest one and was followed by kidney, skeletal muscle, brain, spleen, bone marrow, thymus, and liver. Upon irradiation, alteration of PLD activity was observed in thymus, spleen, lung, and bone marrow. Especially PLD activities of the spleen and thymus revealed the highest sensitivity toward $\gamma-rar$ with more than two times amplification in their activities In contrast, the PLD activity of bone marrow appears to be reduced to nearly $30\%$. Irradiation effect was hardly detected in liver which showed the lowest PLD activity. Conclusion : The PLD activities affected most sensitively by the whole-body irradiation seem to be associated with organs involved in immunity and hematopoiesis. This observation s1ron91y indicates that the PLD is closely related to the physiological function of these organs, Furthermore, radiation stress could offer an important means to explore the phenomena covering from cell Proliferation to cell death on these organs.

• The Korean Journal of Mycology
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• v.13 no.3
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• pp.157-168
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• 1985

To investigate nutritive values of a feed fermented with basidiomycetes, among the isolated strains, Lyophyllum decastes (Fr.) Sing. was found with the greatest enzyme productivity and rapid mycelial growth in rice straw medium. Optimum temperature, pH and moisture content for mycelial growth and enzyme production of the strain were $25{\sim}30^{\circ}C,\;pH\;4.0{\sim}7.0\;and\;70{\sim}75\;%$, respectively. Fifteen days of culture were required for the highest enzyme productivity. Among the sub-materials added, $30{\sim}40\;%$ of rice bran and $10{\sim}20\;%$ of defatted perilla seeds were effective for the enzyme production, but caused a reduced mycelial growth. The greatest effect of an addition of inorganic salts was obtained with $0.36{\sim}0.72\;%\;of\;(NH_4)_2HPO_4$. When 40 mesh or smaller rice straw and steam treatment at $0.5\;kg/cm^2$ were used, the mycelial growth decreased, whereas the enzyme production increased. The mycelial growth and enzyme production increased when $Ca(OH)_2$ was used as the alkali treatment, but decreased with increasing concentration of NaOH. As the fermentation proceeded, the amounts of ash, reducing sugar and total nitrogen increased, but cellulose, lignin and pentosan decreased. When the rice straw was treated with alkali, the amounts of ash, total nitrogen and lignin decreased, but reducing sugar and cellulose increased. At higher NaOH concentration, the variation become greater. The in vitro dry matter digestibility of the products increased from 55.03 % at the beginning of the fermentation to 62.72 % at 45 days after fermentation. The most effective alkali treatment on the digestibility of rice straw was KOH followed by NaOH. However, the digestibility increased with increasing concentration of NaOH. The digestibility of pretreated with alkali increased after fermentation as well.