The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Evaluation of the Physiological Activity and Identification of the Active Ingredients of Crab Apple (Malus prunifolia Borkh.) Extracts

꽃사과(Malus prunifolia Borkh.) 추출물의 생리활성 평가 및 활성 성분의 규명

  • Shin, Hyun Young (Transdisciplinary Major in Learning Health System, Department of Integrated Biomedical and Life Science, Korea University) ;
  • Kim, Hoon (College of Biotechnology and Natural Resources, Chung-Ang University) ;
  • Jeong, Eun-Jin (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Kim, Hyun-Gyeong (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Lee, Kyung-Haeng (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Bae, Yun-Jung (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Kim, Woo Jung (Biocenter, Gyeonggido Business and Science Accelerator) ;
  • Lee, Sanghyun (Dept. of Plant Science and Technology, Chung-Ang University) ;
  • Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
  • 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공) ;
  • 김훈 (중앙대학교 생명공학대학) ;
  • 정은진 (한국교통대학교 식품영양학전공) ;
  • 김현경 (한국교통대학교 식품영양학전공) ;
  • 이경행 (한국교통대학교 식품영양학전공) ;
  • 배윤정 (한국교통대학교 식품영양학전공) ;
  • 김우중 (경기도 경제과학진흥원 바이오센터) ;
  • 이상현 (중앙대학교 식물생명공학과) ;
  • 유광원 (한국교통대학교 식품영양학전공)
  • Received : 2021.08.27
  • Accepted : 2021.09.26
  • Published : 2021.10.31
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Abstract

To utilize Malus pruniforia Borkh. as a functional material, cold-water (CW), hot-water (HW), and 70% ethanol (EtOH) extracts were prepared, and their antioxidant and anti-inflammatory activities were compared. The antioxidant activity of the HW extract evaluated by ABTS and DPPH radical scavenging and FRAP activity was significantly effective. The total polyphenol content of the HW extract was also higher by 15.5±0.7 mg GAE/g extract compared to other extracts. The EtOH extract showed significantly decreased TNF-α (39.8%), IL-6 (65.5%), and NO (34.9%) levels in RAW 264.7 cells compared to the LPS-induced control group. The levels of IL-6 (21.1%) and IL-8 (19.3%) were significantly decreased by treatment of EtOH extract in HaCaT keratinocytes induced with TNF-α and IFN-γ. The UHPLC-MS results indicated that the EtOH extract might have chlorogenic acid and phlorizin as the major compounds. This was validated using HPLC-DAD, which showed that the EtOH extract had higher levels of chlorogenic acid and phlorizin (1,185±58 and 470±10 ㎍/g extract, respectively). In conclusion, the present study suggested that the anti-inflammatory activity of the EtOH extract was more effective than the CW and HW extracts, and chlorogenic acid and phlorizin could be used as indicator compounds and functional substances.

Keywords

Acknowledgement

본 논문은 2021년 농촌진흥청의 지원을 받아 수행한 연구로 이에 감사드립니다(과제번호 PJ0152852021).

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