• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.152-152
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• 2002

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2002년도 Current Trends in Toxicological Sciences
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• pp.90-90
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• 2002

• 대한의생명과학회지
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• 제13권2호
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• pp.91-97
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• 2007

Obesity is defined as increased mass of adipose tissue, conferring a higher risk of cardiovascular and metabolic disorders such as diabetes, hyperlipidemia, and coronary heart disease. To get a better understanding of the role of a male sex hormone testosterone on obesity, we thus measured the effects of testosterone on white adipose tissue (WAT) mass, adipocyte histology and hepatic lipid accumulation in male castrated (CAST) C57BL/6J mice. Compared to male CAST control mice, testosterone-treated mice had the decreased WAT mass and the increased the number of adipocytes. Especially, histological data showed that the adipocyte size was reduced in a dose-dependent manner and was most effective at dose 150 $\mu$g per mouse for testosterone. In addition, the administration of testosterone resulted in the inhibition of hepatic lipid accumulation compared with control mice. Our results suggest that testosterone regulates adipocytes development and hepatic lipid metabolism, resulting in the prevention of obesity in male CAST mice.

• 대한본초학회지
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• 제25권1호
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• pp.33-38
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• 2010

Objectives : This study was designed to investigate therapeutic effects fo NR on allergic asthma in terms of measurement of cytokine profiles, distribution of BAL and expression levels of asthma induced by OVA. Methods : C57BL/6 mouse was divided into normal, NRI, control, and NRII, and Notopterygii Rhizoma Extract was orally administered to the C57BL/6 mice in the normal group and the normal mouse in NRI group while it was orally administered to the control group after inducing asthma by OVA and NRII. The study measured them by means of ELISA after dividing BALF of each group, and analyzed RT-PCR by using lung tissues. Results : In our results, lung weight and the number of total cells in the lung and of Leukocyte and Eosinophils in BALF were significantly decreased. In addition, IL-4, IL-5, IL-13, and IgE in BALF were significantly decreased compared to the control group. Conclusions : These results demonstrate that NR Extract be a desirable altemative therapy for allergic asthma by inhibiting the expressin of immune cells, the activation of inflammatory mediator.

• 약학회지
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• 제43권3호
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• pp.351-357
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• 1999

Hair growth effects of Sangmosu have been evaluated in C57BL/6 mice. Animals were topically treated with Sangmosu (50 or $100{\;}{\mu}l/mouse$) for 40 days on the back of shaved area. Twenty days after treatment, hair growth effects were significantly observed in both female and male mice. There was no sex difference although 30 days after treatment female mice were shown to be more effective at the dose of $50{\;}{\mu}l/mouse$ than males. These data demonstrate that Sangmosu is very effective in hair growth effects in animals and thus it may be applicable to humans.

• Applied Microscopy
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• 제38권1호
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• pp.11-19
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• 2008

전해알칼리환원수가 동물의 면역에 미치는 영향을 알아보고자 본 실험을 수행하였다. 소장에서 기생하는 호르텐스극구흡충(Echinostoma hortense)을 이용하여 C57BL/6 생쥐 소장과 혈액에서의 면역반응을 관찰하였다. C57BL/6 생쥐에 호르텐스극구흡충 피낭유충을 마리 당 15개 경구감염 시킨 후 2주 경과하여 충체를 회수하였고 소장 점막의 배상세포와 비만세포의 변화를 관찰하였으며 protein chip을 이용하여 혈청 내 cytokine의 변화를 비교 확인하였다. 그 결과 호르텐스극구흡충을 감염시킨 군에서는 평균 8.3마리, 알칼리환원 수를 급이한 군에서는 평균 10마리의 충체를 회수하였다. 배상 세포 변화의 관찰에서는 호르텐스극구흡충을 감염시키고 전해알칼리환원수를 급이한 실험군이 융모 당 평균 4.3개 관찰되어 배상세포의 발현이 적은 것으로(p<0.001) 나타났다. 비만세포 변화의 관찰에서도 호르텐스극구흡충을 감염시키고 전해알칼리환원수를 급이한 실험군에서 응모 당 평균 11개 관찰되었고 정수물을 급이한 호르텐스 감염 대조군에 비해 적게 나타났다(p<0.001). 마우스 혈청 내에서의 cytokine 발현에서 호르텐스극구흡충에 감염된 실험군과 대조군의 비교에서 Th1 cytokine인 IL-6, IL-$1{\beta}$, IFN-${\gamma}$, TNF-${\alpha}$, Il-2 등과 Th2 cytokine인 IL-4, IL-5, IL-10, IL-13 등의 발현은 모두 유의한 차이가 관찰되지 않았다. 본 연구에서 전기분해 알칼리환원수가 호르텐스극구흡충의 감염에 있어 C57BL/6 마우스에서 장점막조직의 배상세포 수 및 mucin terminal sugar의 변화를 억제하고 아울러 이로 인해 충체배출이 지연되며, 알칼리환원수가 호르텐스극구흡충에 감염된 C57BL/6 마우스에서 혈청 내 cytokine의 변화에는 큰 영향을 미치지 않는다는 결론을 얻었다.

• 대한수의학회지
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• 제26권2호
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• pp.307-319
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• 1986

성숙한 A/J, $C_{57}BL/6N$, DBA/2 및 NIH-GP계(系) 마우스에 benzo(a) pyrene과 charcoal powder를, $C_{57}/6N$ 및 NIH-GP계(系) 마우스에 Urethan을 기관내(氣管內) 주입(注入)하여 폐장(肺臟)의 종양발생(腫瘍發生)과 조직변화(組織變化)를 관찰(觀察)한 바 다음과 같은 결과(結果)를 얻었다. Benzo(a)pyrene을 주입(注入)한 군(群)에서는 편평상피암(扁平上皮癌) 및 선암(腺癌), 그리고 편평상피암(扁平上皮癌)과 선암(腺癌)의 혼합 발생예(例)를 볼 수 있었다. 이와 같은 병변(病變)은 A/J 및 $C_{57}BL/6N$계(系) 마우스에서 발생률(發生率)이 높았으며 A/J계(系) 마우스에서 편평상피암(扁平上皮癌)의 발생(發生) 및 분화(分化)가 현저하였다. 한편 DBA/2 및 NIH-GP계(系) 마우스에서는 종양(腫瘍) 발생률(發生率)이 극히 낮았다. Benzo(a)pyrene의 주입시(注入時) 동일 계통의 마우스에서도 대량을 주입(注入)할 때 선암(腺癌)보다 편평상피암(扁平上皮癌)의 발생(發生)이 많았으며 A/J계(系) 마우스에서는 편평상피암(扁平上皮癌) 단독(單獨) 발생예(發生例)가 다수 관찰(觀察)되었고 $C_{57}BL/6N$계(系) 마우스에서는 선암(腺癌) 및 선암(腺癌)과 편평상피암(扁平上皮癌)의 혼합 형태(形態)가 주(主)로 관찰(觀察)되었다. 그리고 기관지상피(氣管支上皮)의 편평상피화생(扁平上皮化生) 및 편평상피암(扁平上皮癌)의 발생시(發生時) alcian blue-PAS 양성반응세포가 관여함을 알 수 있었고 선종양조직(腺腫樣組織) 발생예(發生例)에서도 편평상피화생(扁平上皮化生) 및 편평상피암(扁平上皮癌)으로 분화(分化)되는 경우도 관찰(觀察)되었다. Urethan 주입군(注入群)은 극히 낮은 종양발생률(腫瘍發生率)을 나타내어 발암물질(發癌物質)의 종류(種類), 용량(用量) 및 마우스의 계통(系統)에 따라 종양(腫瘍)의 발생률(發生率) 및 형태(形態)의 차이가 인정되었다.

• IMMUNE NETWORK
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• 제4권1호
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• pp.53-59
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• 2004

Background: Minor histocompatibility HY antigen, as a transplantation antigen, has been known to cause graft rejection in MHC (major histocompatibility complex) matched donor-recipient. The aim of our study is to investigate the role of male antigen (HY) disparity on MHC matched pancreatic islet transplantation and to examine the mechanism of the immune reaction. Methods: Pancreatic islets were isolated and purified by collagen digestion followed by Ficoll gradient. The isolated islets of male C57BL6/J were transplanted underneath the kidney capsule of syngeneic female mice rendered diabetic with streptozotocine. Blood glucose was monitored for the rejection of engrafted islets. After certain period of time, tail to flank skin transplantation was performed either on mouse transplanted with HY mismatched islets or on sham treated mouse. The rejection was monitored by scoring gross pathology of the engrafted skin. Results: HY mismatched islets survived more than 300 days in 14 out of 15 mice. The acceptance of second party graft (male B6 islets) and the rejection of third party graft (male BALB/c islets) in these mice suggested the tolerance to islets with HY disparity. B6 Skin with HY disparity was rejected on day $25{\pm}7$. However, HY mismatched skin transplanted on the mice tolerated to HY mismatched islets survived more than 240 days. Tetramer staining in these mice indicated the CTL recognizing MHC Db/Uty was not deleted or anergized. Conclusion: The islet transplantation across HY disparity induced tolerance to HY antigen in C57BL6 mouse, which in turn induced tolerance to HY mismatched skin, which otherwise would be rejected within 25 days. The MHC tetramer staining suggested the underlying mechanisms would not be clonal deletion or anergy.

• Toxicological Research
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• 제22권3호
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• pp.221-228
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• 2006

Bonogen shampoo is composed of several plant extracts which are known to be used in oriental medicine. This study was carried out to investigate the effects of Bonogen shampoo on hair growth in an alopecia model of C57BL/6 mice. There were eight male and female experimental groups including distilled water(DW: negative control), a commercial shampoo[M], 3% minoxidil (MXD) and Bonogen shampoo(BNG). Dorsal skin hair of six-week-old mice was trimmed with an electric clipper carefully not to damage the skin. The next day, mice without skin scratch were selected, randomized and separated in 10 mice per group. The test compounds were topically treated with 0.15 ml per mouse or dorsal skin for 21 days daily and then washed thoroughly with DW. The hair regrowth was determined photographically at 0, 4, 7, 10, 15, 18, and 21 days and histologically at day 21. No clinical signs were observed in all mice. Although body weight was slightly increased in 3% MXD group than other groups, it was not significant. Hair regrowth began to be promoted after 14 days and appeared a distinct regrowth pattern in all animals by topical treatment of test compounds at 18 days. In particular, the topical treatment of bonogen shampoo or 3% MXD for 21 days to dorsal skin accelerated hair regrowth faster than DW or M shampoo. At 21 days, the hair regrowth promotion speed was in order of 3% MXD>BNG>M>DW. The bonogen shampoo or 3% MXD also promoted hair follicle elongation compared to the negative control. These results suggest that bonogen shampoo has hair growth promoting activities and may be useful for treatment of bald or alopecia.

• 생명과학회지
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• 제23권6호
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• pp.779-788
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• 2013

본 연구는 5주간의 고지방 식이(45% 지방)가 복강 대식세포의 면역 반응에 미치는 효과를 분석하였다. C57BL/6 마우스(4주령, N=16)를 2집단으로 나누어 고지방 식이집단(HD, N=8)은 45% 지방 함량 고지방식이를 정상 식이집단(ND, N=8)은 정상식이(10% 지방)를 5주간 실시하였다. 복강 대식세포는 복강세척법을 이용하여 획득하였으며, LPS (lipopolysaccharide, $1{\mu}g/ml$, 24시간)를 이용하여 배양하였다. 체중은 고지방식이 후 유의하게 증가하였으며, LPS 처치 후 HD그룹의 산화질소, IL-$1{\beta}$ 및 IFN-${\gamma}$ 생성량은 ND그룹에 비하여 유의하게 낮았다. 그러나 두 집단에서 TNF-${\alpha}$와 IL-12의 생성량은 유의한 변화가 없었다. 이러한 결과는 비만 시 대식세포의 식균작용, 산화질소, IL-$1{\beta}$ 및 IFN-${\gamma}$ 생성량이 낮아짐에 따라 면역반응과 항원자극 감수성이 저하되는 것으로 사료된다.