Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of High-fat Diet on Peritoneal Macrophage Immunocompetence in C57/BL6 Mice

고지방 식이가 C57/BL6생쥐의 복강대식세포 면역능력에 미치는 영향

  • Jeong, Jun-Hyun (Department of Sports Science, Chungnam National University) ;
  • Park, Hee-Geun (Department of Sports Science, Chungnam National University) ;
  • Lee, Wang-Lok (Department of Sports Science, Chungnam National University)
  • 정준현 (충남대학교 스포츠과학과) ;
  • 박희근 (충남대학교 스포츠과학과) ;
  • 이왕록 (충남대학교 스포츠과학과)
  • Received : 2013.04.03
  • Accepted : 2013.06.19
  • Published : 2013.06.30
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Abstract

This study was to investigate the effect of high-fat diet on macrophage immunocompetence in C57BL/6 mice. C57BL/6 male mice (4 weeks aged, n=16) were divided into two groups. HD groups fed high-fat diet (45% of fat) and ND groups fed chow diet (10% of fat). Peritoneal macrophages were obtained from each mouse intra-peritoneal by sterile lavage method. Macrophage were stimulated with $1{\mu}g/ml$ of lipopolysaccharide (LPS) for 24 hr. Body weight was significantly increased by high-fat diet. Macrophage phagocytosis of HD was significantly lower than that of ND. After 24 hr of LPS stimulation, NO, IL-$1{\beta}$ and IFN-${\gamma}$ production of HD were significantly lower than those of ND. There were no significant differences in the production of TNF-${\alpha}$ and IL-12 between HD and ND. These findings suggest that high fat diet-induced obesity is associated with decreased Immunocompetence and antigen-stimulated sensitivity of peritoneal macrophage, and lower production of NO, IL-$1{\beta}$ and IFN-${\gamma}$ may contribute to these changes.

본 연구는 5주간의 고지방 식이(45% 지방)가 복강 대식세포의 면역 반응에 미치는 효과를 분석하였다. C57BL/6 마우스(4주령, N=16)를 2집단으로 나누어 고지방 식이집단(HD, N=8)은 45% 지방 함량 고지방식이를 정상 식이집단(ND, N=8)은 정상식이(10% 지방)를 5주간 실시하였다. 복강 대식세포는 복강세척법을 이용하여 획득하였으며, LPS (lipopolysaccharide, $1{\mu}g/ml$, 24시간)를 이용하여 배양하였다. 체중은 고지방식이 후 유의하게 증가하였으며, LPS 처치 후 HD그룹의 산화질소, IL-$1{\beta}$ 및 IFN-${\gamma}$ 생성량은 ND그룹에 비하여 유의하게 낮았다. 그러나 두 집단에서 TNF-${\alpha}$와 IL-12의 생성량은 유의한 변화가 없었다. 이러한 결과는 비만 시 대식세포의 식균작용, 산화질소, IL-$1{\beta}$ 및 IFN-${\gamma}$ 생성량이 낮아짐에 따라 면역반응과 항원자극 감수성이 저하되는 것으로 사료된다.

Keywords

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