Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Improvement Effect of Fermented Silkworm (Bombyx mori L.) Powder against Orotic Acid-induced Fatty Liver in Rats

발효누에분말 투여가 orotic acid 유발 흰쥐에 미치는 지방간 개선 효과

  • Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Park, Kyu-Rim (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Kim, Yu-Ra (Department of Biotechnology, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, Dong-A University)
  • 안희영 (동아대학교 대학원 의생명과학과) ;
  • 차재영 (대선주조(주)기술연구소) ;
  • 박규림 (동아대학교 대학원 의생명과학과) ;
  • 김유라 (동아대학교 생명공학과) ;
  • 조영수 (동아대학교 생명공학과)
  • Received : 2013.04.23
  • Accepted : 2013.06.21
  • Published : 2013.06.30
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Abstract

Current study was to investigate the potential effects of silkworm, Bombyx mori L. fermented with Bacillus subtilis (BFSP) and Aspergillus kawachii (AFSP) at the 5% (w/w) levels in Sprague-Dawley rats, which was intoxicated with 1% (w/w) orotic acid (OA) for 10 days. The rat group administered silkworm powder showed improvements in fatty liver condition. Consumption of fermented silkworm powder reduced triglyceride concentrations in the liver tissues and serum and increased the serum lipid concentrations to normal levels, thereby aiding in improving fatty liver conditions. These effects were more pronounced in the BFSP than that in SP or AFSP in orotic acid-induced hepatotoxicity and oxidative stress. Based on these results, fermented silkworms are considered to be a material with significant potential for development into a functional health food that can improve fatty liver conditions.

단백질 분해 활성, 이화학적 특성 및 생리활성을 검토한 결과 우수한 효능을 나타낸 Bacillus subtilis 및 Aspergillus kawachii 균주를 이용하여 Orotic acid-유발 지방간의 개선 효과를 알아보기 위해 발효된 누에 분말을 식이에 5% 수준으로 첨가하였다. 발효 누에 분말은 Orotic acid 지방간 유발 흰쥐의 간 조직 및 혈청 중성지질 농도를 낮춰주고, 혈청 지질농도를 정상 수준으로 올려주어 지방간 개선에 도움을 주었다. SP 투여군과 AFSP 투여군에 반해, BFSP 투여군에서 지질대사와 관련된 개선 효과를 보였고, 간 조직의 병리학적 관찰결과에서도 BFSP 투여군이 간소엽을 구성하고 있는 간 세포들이 전반적으로 균일하게 배열되어 있어 정상군(N)의 간 소견과 비슷한 양상을 보여 지방간의 개선효과가 있는 것으로 보였다. 이러한 결과를 바탕으로 발효 누에 분말은 향후 지방간 개선효능을 가지는 건강식품 개발 가능성이 높은 소재로 판단되어 진다.

Keywords

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