• Korean Journal of Acupuncture
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• v.30 no.4
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• pp.212-219
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• 2013

Objectives : Cirsium japonicum is a traditional Korean medicine that has been used in the treatment of inflammatory diseases such as appendicitis, hepatitis, pulmonary abscess and tumor. The aim of study was to elucidate anti-migratory activity of CJP(Cirsium japonicum pharmacopuncture) through regulation of inflammatory mediators in C6 glioma cell. Methods : Nitric oxide(NO) production was determined by using nitrite assay. The cell migration was analyzed by wound-healing assay and Boyden chamber assay. The expression levels of iNOS, and protein kinase C(PKC)-${\alpha}$ were measured by western blotting assay. Results : CJP showed a significant decrease on NO production. Moreover, glioma cell migration was effectively suppressed by CJP. Furthermore, CJP inhibited the expressions of iNOS and PKC-${\alpha}$ in C6 glioma cells. Conclusions : These results suggest that CJP inhibits glioma cell migration and iNOS expression through regulation of PKC-${\alpha}$. Therefore, it is expected that CJP could be an effective agents for blocking malignant progression of glioma.

• Applied Biological Chemistry
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• v.29 no.1
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• pp.36-43
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• 1986

In order to produce cellulosic single cell protein from the cellulose, 163 strains of cellulose assimilating bacteria were isolated from 95 sources and one of them was screened by its strong cellulose assimilating activity. and was identified as Sporocytophaga congregate A-7. The optimum temperature and pH for cellulase production were $30^{\circ}C$ and 6.0, and the optimum temperature, pH and heat stability of the enzyme were $50^{\circ}C$, 7.0 and below $55^{\circ}C$. When the bacteria was cultured in fermentor, the specific growth rate was $0.034hr^{-1}$ and when the bacteria was mixed cultured with Candida guilliermondii var. guilliermondii, the specific growth rate of the bacteria and yeast were $0.06hr^{-1}$ and $0.08hr^{-1}$ respectively and total cell dry weight was $4{\sim}5g/l$.

• Biomolecules & Therapeutics
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• v.25 no.2
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• pp.158-164
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• 2017

Methylglyoxal (MGO) is a highly reactive metabolite of glucose which is known to cause damage and induce apoptosis in endothelial cells. Endothelial cell damage is implicated in the progression of diabetes-associated complications and atherosclerosis. Hypericin, a naphthodianthrone isolated from Hypericum perforatum L. (St. John's Wort), is a potent and selective inhibitor of protein kinase C and is reported to reduce neuropathic pain. In this work, we investigated the protective effect of hypericin on MGO-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Hypericin showed significant anti-apoptotic activity in MGO-treated HUVECs. Pretreatment with hypericin significantly inhibited MGO-induced changes in cell morphology, cell death, and production of intracellular reactive oxygen species. Hypericin prevented MGO-induced apoptosis in HUVECs by increasing Bcl-2 expression and decreasing Bax expression. MGO was found to activate mitogen-activated protein kinases (MAPKs). Pretreatment with hypericin strongly inhibited the activation of MAPKs, including P38, JNK, and ERK1/2. Interestingly, hypericin also inhibited the formation of AGEs. These findings suggest that hypericin may be an effective regulator of MGO-induced apoptosis. In conclusion, hypericin downregulated the formation of AGEs and ameliorated MGO-induced dysfunction in human endothelial cells.

• Journal of the Korean Chemical Society
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• v.53 no.1
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• pp.34-41
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• 2009

A new series of hydrazone derivatives were synthesized from 4-(2-chloroethyl)semicarbazide and their antiproliferative activity against human brain (U251) and liver (Hepg2) carcinoma cell lines were evaluated. The hydrazone compounds are benzaldehyde (2a-2g), acetophenone (3a-3f), and 3-formylindole derivatives (4a-4d). Among the acetophenone derivatives, 3e (p-methoxy substituted) and 3f (p-nitro substituted) showed the highest cytotoxic activity against Hepg2 cell line (I$C_{50}$ = 6 and 8 $\mu$g/ml, respectively). Among the 3-formylindole derivatives, 4a (hydrazone of 3-formylindole itself) showed a pronounced cytotoxic activity against both U251 (I$C_{50}$ = 21 $\mu$g/ml) and Hepg2 (I$C_{50}$ = 7 $\mu$g/ml).

• Transactions of the Korean hydrogen and new energy society
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• v.23 no.1
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• pp.34-42
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• 2012

Gas diffusion layers (GDLs) of carbon composite type in polymer electrolyte fuel cells were prepared by simple and cheap manufacturing process. To obtain the carbon composite GDLs, carbon black with polymer binder was mixed in solvent, rolled to make sheet, and finally heat-treated at $340^{\circ}C$. The performance of fuel cell using composite GDLs was changed by PTFE content. The physical properties of composite GDLs for pore, conductivity and air permeability were analyzed to compare with the variation of fuel cell performance. The conductivity of composite GDLs was very similar to carbon paper as commercial GDL but pore properties and air flux were considerably different. The porosity, PTFE content and conductivity for composite GDLs did not have an influence on the cell performance much. The increase of pore diameter and air flux led to enhance cell performance.

• Resources Recycling
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• v.21 no.2
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• pp.34-40
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• 2012

$Pt_{10}$/C, $Pt_9Mn_1$/C, $Pt_7Mn_3$/C electrocatalysts for Polymer Electrolyte Membrane Fuel Cells(PEMFCs) were synthesized by reduction with HCHO and their activity as a oxygen reduction reaction(ORR) was examined at half cell. The electrochemical oxygen reduction reaction(ORR) was studied by using a glaasy carbon electrode through cyclic voltammetric curves(CV) in a 1 M $H_2SO_4$ solution. The ORR activities of $Pt_9Mn_1$/C were higher than $Pt_{10}$/C, $Pt_7Mn_3$/C. Also potential-current curves of $Pt_9Mn_1$/C at 0.9, 0.8, 0.7, 0.6V for 5minutes respectively were higher than $Pt_{10}$/C, $Pt_7Mn_3$/C. Physical characterization was made by using x-ray diffraction(XRD) and transmission electron microscope(TEM). The TEM images of $Pt_9Mn_1$/C, $Pt_{10}$/C catalysts showed homogenous particle distribution with particle size of about 2.7 nm, 3 nm respectively and then the XRD results showed that the crystalline structure of the synthesized catalysts are seen FCC structure.

• Bulletin of the Korean Chemical Society
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• v.13 no.5
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• pp.491-498
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• 1992

Chelating triphosphines were applied to freeze the fluxionality and to minimize the number of isomers found in the monophosphine analogues and this technique was proved to be useful. RuH(NO)$P_3$($P_3$; Cyttp, ttp and etp) complexes were characterized to have similar trigonal bipyramidal structures with linear NO groups. Cyttp prefers to have a meridional geometry while etp prefers a facial one and ttp complexes are mixture of these two isomers. The crystal structure of RuH(NO)(Cyttp) has been determined to have a distorted trigonal bipyramidal structure with a linear NO in the equatorial plane. The crystals are orthorhombic, space group $P_{nma}$, with unit cell dimensions a = 16.356(2), b = 20.474(2), c = 10.915(l) ${\AA}$, V = 3655 ${\AA}^3$, Z = 4, R = 0.035 and $R_w$ = 0.034 for the 2900 intensities with $F_o^2 >3{\sigma}(F_o^2)$ and the 208 variables.

• Bulletin of the Korean Chemical Society
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• v.10 no.4
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• pp.349-352
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• 1989

Two Crystal structures of fully dehydrated partially magnesium exchanged zeolite A, stoichiometries of $Mg_{2.5}Na_7-A$ (a = 12.251 (1) ${\AA}$) and $Mg_{1.5}Na_9-A\; (a\;=\;12.214(1)\;{\AA})$ per unit cell, have been determined from the 3-dimensional X-ray diffraction data gathered by counter methods. All structures were solved and refined in the cubic space group Pm3m at 21(1)$^{\circ}C$. The structures of the dehydrated $Mg_{2.5}Na_7-A$ and $Mg_{1.5}Na_9-A$ were refined to yield the final error indices $R_1$ = 0.042 and $R_2$ = 0.049 with 318 reflections, and $R_1$ = 0.034 and $R_2$ = 0.032 with 252 reflections, respectively, for which I > $3{\sigma}(I)$. Both structures indicate that $Mg^{2+}$ ions are coordinated by three framework oxygens and the angle substended at $Mg^{2+}$ ions, O(3)-Mg(1)-O(3) is ca. $120^{\circ}$, close to the idealized trigonal planar value. $Mg^{2+}$ ions preferentially occupy 6-ring sites and $Na^+$ ions occupy 8-ring sites when total number of cations per unit cell is more than 8.

• KSBB Journal
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• v.5 no.4
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• pp.355-363
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• 1990

Methanol-utilizing bacterium isolated from sewage samples in Seoul showed optimal temperature and pH of $33^{\circ}C$ and 7.1 for growth, respectively. The maximum specific growth rate was $0.42hr^{-1}$. The minimum medium composition was reconstituted depending on the surplus and the deficit of each component in the basal medium at steady state. The optimal composition was given as(g/l); Methanol 40, $(NH_4)_2\;SO_42, \;KH_2PO_4\;1.5, \;K_2HPO_4\;0.2, \;H_3PO_4\;0.79, \;Na_2HPO_4{\cdot}12H_2O\;0.15, \;MgSO_4{\cdot}7H_2O\;1.5, \;FeSO_4{\cdot}7H_2O\;0.034, \;MnSO_4{\cdot}4H_2O\;0.005, \;CuSO_4{\cdot}5H_2O\;0.0027, \;CaCl_2{\cdot}2H_2O\;0.25, \;ZnSO_4{\cdot}7H_2O\;0.007, \;(NH_4)_6\;Mo_7O_{24}{\cdot}4H_2O\;0.00048, \;H_3BO_3\;0.00068, \;CoCl_2\; 0.00024$ Under the continuous culture with optimum medium the maximum cell productivity was 3.8g/1/hr at dilution rate $0.23hr^{-1}$. Maximum cell concentration and its protein content were 19.5g/l and 70% at dilution rate of $0.1hr^{-1}$, respectively.

• Biomolecules & Therapeutics
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• v.21 no.5
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• pp.371-380
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• 2013

Doxorubicin is still main drug in chemotherapy with limitation of use due to adverse drug reaction. Increased oxidative stress and alteration of nitric oxide control have been involved in cardiotoxicity of doxorubicin (DOX). A Disintegrin And Metalloproteinase (ADAMs) are transmembrane ectoproteases to regulate cell-cell and cell-matrix interactions, but role in cardiac disease is unclear. The aim of this study was to determine whether DOX activates peroxynitrite and ADAM 10 and thus ADAM and matrix metalloproteinase (MMP) induce cardiac remodeling in DOX-induced cardiomyopathy. Adult male Sprague-Dawley rats were subjected to cardiomyopathy by DOX (6 times of 2.5 mg/kg DOX over 2-weeks), and were randomized as four groups. Then followed by 3, 5, 7, and 14 days after cessation of DOX injection. DOX-injected animals significantly decreased left ventricular fractional shortening compared with control by M-mode echocardiography. The expressions of cardiac nitrotyrosine by immunohistochemistry were significant increased, and persisted for 2 weeks following the last injection. The expression of eNOS was increased by 1.9 times (p<0.05), and iNOS was marked increased in DOX-heart compared with control (p<0.001). Compared to control rats, cardiac ADAM10- and MMP 9- protein expressions increased by 20 times, and active/total MMP 9 proteolytic activity showed increase tendency at day 14 after cessation of DOX injection (n=10, each group). DOX-treated $H_9C_2$ cell showed increased ADAM10 protein expression with dose-dependency (p<0.01) and morphometric changes showed the increase of ventricular interstitial, nonvascular collagen deposition. These data suggest that activation of cardiac peroxynitrite with increased iNOS expression and ADAM 10-dependent MMP 9 expression may be a molecular mechanism that contributes to left ventricular remodeling in DOXinduced cardiomyopathy.