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The Anti-Migratory Effect of Cirsium japonicum Pharmacopuncture in C6 Glioma Cell (대계 약침액의 C6 신경교종 세포에 대한 이주 억제 효과)

  • Park, Juyeon;Lee, Kangpa;Chang, Haeryong;Moon, Jinyoung
    • Korean Journal of Acupuncture
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    • v.30 no.4
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    • pp.212-219
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    • 2013
  • Objectives : Cirsium japonicum is a traditional Korean medicine that has been used in the treatment of inflammatory diseases such as appendicitis, hepatitis, pulmonary abscess and tumor. The aim of study was to elucidate anti-migratory activity of CJP(Cirsium japonicum pharmacopuncture) through regulation of inflammatory mediators in C6 glioma cell. Methods : Nitric oxide(NO) production was determined by using nitrite assay. The cell migration was analyzed by wound-healing assay and Boyden chamber assay. The expression levels of iNOS, and protein kinase C(PKC)-${\alpha}$ were measured by western blotting assay. Results : CJP showed a significant decrease on NO production. Moreover, glioma cell migration was effectively suppressed by CJP. Furthermore, CJP inhibited the expressions of iNOS and PKC-${\alpha}$ in C6 glioma cells. Conclusions : These results suggest that CJP inhibits glioma cell migration and iNOS expression through regulation of PKC-${\alpha}$. Therefore, it is expected that CJP could be an effective agents for blocking malignant progression of glioma.

Production and Characteristics of Cellulase from Sporocytophaga congregata and Mixed Culture with Yeast (Sporocytophaga congregata에 의(依)한 Cellulase 생산(生産) 및 그 효소특성(酵素特性)과 효모(酵母)와의 혼합배양(混合培養))

  • Kim, Chang-Jin;Kim, Sang-Soon;Lee, Ke-Ho
    • Applied Biological Chemistry
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    • v.29 no.1
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    • pp.36-43
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    • 1986
  • In order to produce cellulosic single cell protein from the cellulose, 163 strains of cellulose assimilating bacteria were isolated from 95 sources and one of them was screened by its strong cellulose assimilating activity. and was identified as Sporocytophaga congregate A-7. The optimum temperature and pH for cellulase production were $30^{\circ}C$ and 6.0, and the optimum temperature, pH and heat stability of the enzyme were $50^{\circ}C$, 7.0 and below $55^{\circ}C$. When the bacteria was cultured in fermentor, the specific growth rate was $0.034hr^{-1}$ and when the bacteria was mixed cultured with Candida guilliermondii var. guilliermondii, the specific growth rate of the bacteria and yeast were $0.06hr^{-1}$ and $0.08hr^{-1}$ respectively and total cell dry weight was $4{\sim}5g/l$.

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Dual CRLH Based Band Stop Filter Using Conductor-Backed Defected Coplanar Waveguide

  • Yang, Doo-Yeong;Yang, Lei
    • International Journal of Contents
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    • v.11 no.3
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    • pp.34-38
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    • 2015
  • A band stop filter is proposed with cascading unit cells that are based on a dual composite right/left-handed (D-CRLH) conductorbacked coplanar waveguide. The parameters of the unit cell have been analyzed to confirm the behavior of each component for the equivalent circuit of the cell. We simulated the dispersion characteristics and energy distribution and have determined that the unit cell has a D-CRLH property. The band stop filter was implemented by symmetrically cascading two of the proposed unit cells. The experimental results for the band stop filter revealed a band rejection performance of 32 dB and a return loss of 0.35 dB in the stopband frequency range from 869MHz to 954MHz. Finally, we show that there is a good agreement in the experimental results and those obtained through the simulations.

Hypericin, a Naphthodianthrone Derivative, Prevents Methylglyoxal-Induced Human Endothelial Cell Dysfunction

  • Do, Moon Ho;Kim, Sun Yeou
    • Biomolecules & Therapeutics
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    • v.25 no.2
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    • pp.158-164
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    • 2017
  • Methylglyoxal (MGO) is a highly reactive metabolite of glucose which is known to cause damage and induce apoptosis in endothelial cells. Endothelial cell damage is implicated in the progression of diabetes-associated complications and atherosclerosis. Hypericin, a naphthodianthrone isolated from Hypericum perforatum L. (St. John's Wort), is a potent and selective inhibitor of protein kinase C and is reported to reduce neuropathic pain. In this work, we investigated the protective effect of hypericin on MGO-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Hypericin showed significant anti-apoptotic activity in MGO-treated HUVECs. Pretreatment with hypericin significantly inhibited MGO-induced changes in cell morphology, cell death, and production of intracellular reactive oxygen species. Hypericin prevented MGO-induced apoptosis in HUVECs by increasing Bcl-2 expression and decreasing Bax expression. MGO was found to activate mitogen-activated protein kinases (MAPKs). Pretreatment with hypericin strongly inhibited the activation of MAPKs, including P38, JNK, and ERK1/2. Interestingly, hypericin also inhibited the formation of AGEs. These findings suggest that hypericin may be an effective regulator of MGO-induced apoptosis. In conclusion, hypericin downregulated the formation of AGEs and ameliorated MGO-induced dysfunction in human endothelial cells.

Incorporating Erlotinib or Irinotecan Plus Cisplatin into Chemoradiotherapy for Stage III Non-small Cell Lung Cancer According to EGFR Mutation Status

  • Lee, Youngjoo;Han, Ji-Youn;Moon, Sung Ho;Nam, Byung-Ho;Lim, Kun Young;Lee, Geon Kook;Kim, Heung Tae;Yun, Tak;An, Hye Jin;Lee, Jin Soo
    • Cancer Research and Treatment
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    • v.49 no.4
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    • pp.981-989
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    • 2017
  • Purpose Concurrent chemoradiotherapy (CCRT) is the standard care for stage III non-small cell lung cancer (NSCLC) patients; however, a more effective regimen is needed to improve the outcome by better controlling occult metastases. We conducted two parallel randomized phase II studies to incorporate erlotinib or irinotecan-cisplatin (IP) into CCRT for stage III NSCLC depending on epidermal growth factor receptor (EGFR) mutation status. Materials and Methods Patients with EGFR-mutant tumors were randomized to receive three cycles of erlotinib first and then either CCRT with erlotinib followed by erlotinib (arm A) or CCRT with IP only (arm B). Patients with EGFR unknown or wild-type tumors were randomized to receive either three cycles of IP before (arm C) or after CCRT with IP (arm D). Results Seventy-three patients were screened and the study was closed early because of slow accrual after 59 patients were randomized. Overall, there were seven patients in arm A, five in arm B, 22 in arm C, and 25 in arm D. The response rate was 71.4% and 80.0% for arm A and B, and 70.0% and 73.9% for arm C and D. The median overall survival (OS) was 39.3 months versus 31.2 months for arm A and B (p=0.442), and 16.3 months versus 25.3 months for arm C and D (p=0.050). Patients with sensitive EGFR mutations had significantly longer OS than EGFR-wild patients (74.8 months vs. 25.3 months, p=0.034). There were no unexpected toxicities. Conclusion Combined-modality treatment by molecular diagnostics is feasible in stage III NSCLC. EGFR-mutant patients appear to be a distinct subset with longer survival.

Synthesis of Hydrazone Derivatives of 4-(2-Chloroethyl) semicarbazide : A New Class of Cytotoxic Agents (4-(2-Chloroethyl) semicarbazide의 히드라존 유도체 합성:새로운 종류의 세포독성요법제)

  • El-Sabbagh, O.I.;El-Sadek, M.E.;Aboukull, M.E.;Shallal, H.M.
    • Journal of the Korean Chemical Society
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    • v.53 no.1
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    • pp.34-41
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    • 2009
  • A new series of hydrazone derivatives were synthesized from 4-(2-chloroethyl)semicarbazide and their antiproliferative activity against human brain (U251) and liver (Hepg2) carcinoma cell lines were evaluated. The hydrazone compounds are benzaldehyde (2a-2g), acetophenone (3a-3f), and 3-formylindole derivatives (4a-4d). Among the acetophenone derivatives, 3e (p-methoxy substituted) and 3f (p-nitro substituted) showed the highest cytotoxic activity against Hepg2 cell line (I$C_{50}$ = 6 and 8 $\mu$g/ml, respectively). Among the 3-formylindole derivatives, 4a (hydrazone of 3-formylindole itself) showed a pronounced cytotoxic activity against both U251 (I$C_{50}$ = 21 $\mu$g/ml) and Hepg2 (I$C_{50}$ = 7 $\mu$g/ml).

Preparation and Characterization for Carbon Composite Gas Diffusion Layer on Polymer Electrolyte Membrane Fuel Cells (고분자 전해질 연료전지에서 탄소복합 기체확산층의 제조와 특성분석)

  • Shim, Joong-Pyo;Han, Choon-Soo;Sun, Ho-Jung;Park, Gyung-Se;Lee, Ji-Jung;Lee, Hong-Ki
    • Transactions of the Korean hydrogen and new energy society
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    • v.23 no.1
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    • pp.34-42
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    • 2012
  • Gas diffusion layers (GDLs) of carbon composite type in polymer electrolyte fuel cells were prepared by simple and cheap manufacturing process. To obtain the carbon composite GDLs, carbon black with polymer binder was mixed in solvent, rolled to make sheet, and finally heat-treated at $340^{\circ}C$. The performance of fuel cell using composite GDLs was changed by PTFE content. The physical properties of composite GDLs for pore, conductivity and air permeability were analyzed to compare with the variation of fuel cell performance. The conductivity of composite GDLs was very similar to carbon paper as commercial GDL but pore properties and air flux were considerably different. The porosity, PTFE content and conductivity for composite GDLs did not have an influence on the cell performance much. The increase of pore diameter and air flux led to enhance cell performance.

Effects of PtMn composition on carbon supported PtMn catalysts for PEMFC (Mn조성비(組成比)가 PEMFC용(用) Pt/C 전극촉매(電極觸媒) 특성(特性)에 미치는 영향(影響)에 관(關)한 연구(硏究))

  • Yoo, Sung-Yeol;Kang, Suk-Min;Lee, Jin-A;Rhee, Choong-Kyun;Ryu, Ho-Jin
    • Resources Recycling
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    • v.21 no.2
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    • pp.34-40
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    • 2012
  • $Pt_{10}$/C, $Pt_9Mn_1$/C, $Pt_7Mn_3$/C electrocatalysts for Polymer Electrolyte Membrane Fuel Cells(PEMFCs) were synthesized by reduction with HCHO and their activity as a oxygen reduction reaction(ORR) was examined at half cell. The electrochemical oxygen reduction reaction(ORR) was studied by using a glaasy carbon electrode through cyclic voltammetric curves(CV) in a 1 M $H_2SO_4$ solution. The ORR activities of $Pt_9Mn_1$/C were higher than $Pt_{10}$/C, $Pt_7Mn_3$/C. Also potential-current curves of $Pt_9Mn_1$/C at 0.9, 0.8, 0.7, 0.6V for 5minutes respectively were higher than $Pt_{10}$/C, $Pt_7Mn_3$/C. Physical characterization was made by using x-ray diffraction(XRD) and transmission electron microscope(TEM). The TEM images of $Pt_9Mn_1$/C, $Pt_{10}$/C catalysts showed homogenous particle distribution with particle size of about 2.7 nm, 3 nm respectively and then the XRD results showed that the crystalline structure of the synthesized catalysts are seen FCC structure.

Chemistry of Ruthenium Hydridonitrosyl Complexes Containing Chelating Triphosphines I-Structures of RuH(NO)$P_3$ ($P_3$ : Chelating Triphosphines)

  • Ik Mo Lee;Devon W. Mee;Judith Gallucci
    • Bulletin of the Korean Chemical Society
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    • v.13 no.5
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    • pp.491-498
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    • 1992
  • Chelating triphosphines were applied to freeze the fluxionality and to minimize the number of isomers found in the monophosphine analogues and this technique was proved to be useful. RuH(NO)$P_3$($P_3$; Cyttp, ttp and etp) complexes were characterized to have similar trigonal bipyramidal structures with linear NO groups. Cyttp prefers to have a meridional geometry while etp prefers a facial one and ttp complexes are mixture of these two isomers. The crystal structure of RuH(NO)(Cyttp) has been determined to have a distorted trigonal bipyramidal structure with a linear NO in the equatorial plane. The crystals are orthorhombic, space group $P_{nma}$, with unit cell dimensions a = 16.356(2), b = 20.474(2), c = 10.915(l) ${\AA}$, V = 3655 ${\AA}^3$, Z = 4, R = 0.035 and $R_w$ = 0.034 for the 2900 intensities with $F_o^2 >3{\sigma}(F_o^2)$ and the 208 variables.

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Two Crystal structures of Fully Dehydrated Partially Magnesium Exchanged Zeolite A, $Mg_{1.5}Na_9-A\;and\;Mg{2.5}Na_7$-A

  • Kim, Yang;Lee, Suk-Hee;Park, Jong-Yul;Kim, Un-Sik
    • Bulletin of the Korean Chemical Society
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    • v.10 no.4
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    • pp.349-352
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    • 1989
  • Two Crystal structures of fully dehydrated partially magnesium exchanged zeolite A, stoichiometries of $Mg_{2.5}Na_7-A$ (a = 12.251 (1) ${\AA}$) and $Mg_{1.5}Na_9-A\; (a\;=\;12.214(1)\;{\AA})$ per unit cell, have been determined from the 3-dimensional X-ray diffraction data gathered by counter methods. All structures were solved and refined in the cubic space group Pm3m at 21(1)$^{\circ}C$. The structures of the dehydrated $Mg_{2.5}Na_7-A$ and $Mg_{1.5}Na_9-A$ were refined to yield the final error indices $R_1$ = 0.042 and $R_2$ = 0.049 with 318 reflections, and $R_1$ = 0.034 and $R_2$ = 0.032 with 252 reflections, respectively, for which I > $3{\sigma}(I)$. Both structures indicate that $Mg^{2+}$ ions are coordinated by three framework oxygens and the angle substended at $Mg^{2+}$ ions, O(3)-Mg(1)-O(3) is ca. $120^{\circ}$, close to the idealized trigonal planar value. $Mg^{2+}$ ions preferentially occupy 6-ring sites and $Na^+$ ions occupy 8-ring sites when total number of cations per unit cell is more than 8.

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