Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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The Anti-Migratory Effect of Cirsium japonicum Pharmacopuncture in C6 Glioma Cell

대계 약침액의 C6 신경교종 세포에 대한 이주 억제 효과

  • Park, Juyeon (Department of Acupoint, College of Korean Medicine, Dongguk University) ;
  • Lee, Kangpa (Division of Bio Science, College of Science and Technology, Dongguk University) ;
  • Chang, Haeryong (Division of Bio Science, College of Science and Technology, Dongguk University) ;
  • Moon, Jinyoung (Department of Acupoint, College of Korean Medicine, Dongguk University)
  • 박주연 (동국대학교 한의과대학 경혈학교실) ;
  • 이강파 (동국대학교 과학기술대학 세포유전학 실험실) ;
  • 장해룡 (동국대학교 과학기술대학 세포유전학 실험실) ;
  • 문진영 (동국대학교 한의과대학 경혈학교실)
  • Received : 2013.10.24
  • Accepted : 2013.11.25
  • Published : 2013.12.27
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Abstract

Objectives : Cirsium japonicum is a traditional Korean medicine that has been used in the treatment of inflammatory diseases such as appendicitis, hepatitis, pulmonary abscess and tumor. The aim of study was to elucidate anti-migratory activity of CJP(Cirsium japonicum pharmacopuncture) through regulation of inflammatory mediators in C6 glioma cell. Methods : Nitric oxide(NO) production was determined by using nitrite assay. The cell migration was analyzed by wound-healing assay and Boyden chamber assay. The expression levels of iNOS, and protein kinase C(PKC)-${\alpha}$ were measured by western blotting assay. Results : CJP showed a significant decrease on NO production. Moreover, glioma cell migration was effectively suppressed by CJP. Furthermore, CJP inhibited the expressions of iNOS and PKC-${\alpha}$ in C6 glioma cells. Conclusions : These results suggest that CJP inhibits glioma cell migration and iNOS expression through regulation of PKC-${\alpha}$. Therefore, it is expected that CJP could be an effective agents for blocking malignant progression of glioma.

Keywords

References

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