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Effects of Yellow Clay on the Production of Volatile Fatty Acids during the Anaerobic Decomposition of the Red Tide Dinoflagellate Cochlodinium polykrikoides in Marine Sediments (해양퇴적층에서 적조생물(Cochlodinium polykrikoides)의 혐기성 분해과정 중 황토가 휘발성 지방산 생성에 미치는 영향)

  • Park, Young-Tae;Lee, Chang-Kyu;Park, Tae-Gyu;Lee, Yoon;Bae, Heon-Meen
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.45 no.5
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    • pp.472-479
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    • 2012
  • The formation of volatile fatty acids(VFAs) and changes in pH, oxidation and reduction potential(Eh) and acid volatile sulfide(AVS) with the addition of yellow clay were investigated using microcosm systems to examine the effects of yellow clay dispersion on the anaerobic decomposition of Cochlodinium polykrikoides in marine sediments. The acetate concentration reached a maximum by day 4 and was 1.2-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (224-270 vs. 333 uM). The formate concentration reached a maximum by day 1 and was 1.3-2.8 fold less in the sample treated with yellow clay compared to the untreated sample (202-439 vs. 563 uM). The propionate concentration reached a maximum by day 2 and was 1.5-1.8 fold less in the sample treated with yellow clay compared to the untreated sample (32.6 vs. 57.2 uM). After the amounts of acetate, formate and propionate peaked the levels dropped dramatically due to the utilization by sulfate reducing bacteria. The Eh of the samples treated with yellow clay was similar to the untreated sample on day 0 but was higher in the sample treated with yellow clay(140-206 mV) from days 4 to 17. AVS started to form on day 3 and this was sustained until day 6, and 1.2-2.2 fold less was produced in the sample treated with yellow clay compared to the untreated sample (40.2-69.3 vs. 83.2-93.8 mg/L). Accordingly, during the anaerobic decomposition of C. polykrikoides in marine sediments, yellow clay dispersal seems to suppress the reduction state of Eh and the formation of volatile fatty acids(acetate, formate and propionate) used as an energy source by sulfate reducing bacteria, indicating that this process controls the production of hydrogen sulfide that negatively affects marine organisms and the marine sediment environment.

Analytical Study of Delay Model of Traffic Signal Progression Evaluation on Arterial (간선도로 신호연동화 효율의 평가를 위한 지체도 모형의 해석적 연구)

  • 김영찬;황연하
    • Journal of Korean Society of Transportation
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    • v.17 no.1
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    • pp.131-139
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    • 1999
  • The quality of progression at signalized intersection has the largest potential effect. TRANSYT-7F is widely used to estimate the signal progression delay, but the progress of collecting and executing the compute program appears to be rather cumbersome. The research is to develop the analytical and progressing platoon delay model that is as simple as the methodology of HCM and familiar with the output of simulation model. The general approach to this research was conducted to examine the Rouphail and NCHRP 339 methodology together with the existing progression delay model (TRANSYT-7F. HCM). The scope is contained to be applicable only to cycles with no overflow queue and to obtain a comprehensive evaluation of the effects of changes in the quality of traffic signal progression on stopped delay and to be analyzed a simple mathematical method. The principle assumption for this model is that secondary flows is dispersed and partly mixed with average flow of the primary progressed flow. A second assumption is that through flow is consisted with the part of saturation flow at the front of it and the part of average flow at the rear of it. The delay equations vary for two arrival. The conclusion of this study could be summarized as 1)The evaluation of this model was consistently similar to that of TRANSYT-7F, 2) Platoon pattern has the real traffic flow characteristics. 3) The computing process of progression delay is made to have simple logic and easy calculation by integration, 4) This model could be estimated to be applied in almost all case.

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Agrobacterium-Mediated Transformation on a Plant with Saccharomyces cerevisiae Acid Phosphatse Gene(PHO5) (Agrobacterium을 이용한 Saccharomyces cerevisiae Acid Phosphatse 유전자 (PHO5) 의 식물체로의 도입)

  • Ki yong Kim;Dae yuong Son;Yong Gu Park;Won Il Jung;Jin Ki Jo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.13 no.3
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    • pp.177-183
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    • 1993
  • This study was conducted to obtain the transformed tobacco plants with S. cerevisiae Acid phosphatase gene(PH05) using Agrobacterium tumefaciens and th confirm plant transformation and gene expression. the results obtained were summarized as follows: APase activity of Saccharomyces cereviase NA 87-11A was remarkably showed up as deep red color when assayed by Tohe and Oshima(1974). PH05 fragment, Apase gene, was obtained from pVC727G and the graphically estimated size was about 1.5kb by agarose gel electrophoresis. The sequencing results of 5'end and 3'end of PH05 using dideoxy chain termination method were coinsided with the full length nucleotide already. pBKJ I vector was constructed by isolation of PH05 fragment from pVC727-1 and pBKSI-1 digesred with Sma I and Xba I. Isolated plasmid from transformed A. tumefaciens with constructed pBKJ I when it was electrophoresed with agarose gel. The dosc of tobacco leaf was cocultivated 재소 transformed Agronacterium tumefaciens. Transformed shoots were selected on kanamtcin-containing MS-n/B medium and they were regenerated. The transgenic tobacco plants were elucidated by isolation of genomic DNA and genomic southern hybridization using ${\alpha}-^{32}P$ labelled PH05 fragments. The PH05 in transformed tobacco plants was expressed in leaf, stem and root, and its APase activity was estimated as deep red color by Tohe method.

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Development of an Actuated Traffic Signal Control Strategy to Minimize Dilemma Zone (딜레마 구간 최소화를 위한 감응식 신호제어전략의 개발)

  • Kim Youngchan;Huh Jung Ah
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.1 no.1
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    • pp.58-69
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    • 2002
  • Most of the traffic accidents are a rear-end collision and a clash generated in the signalized intersection on the local roads. So, it is demanded that the high-quality of signal control and dilemma zone control. According to the cases generated by foreign countries, we established the strategies which are composed of Volume-Density Control, strategy of the dilemma zone control using R-detector (microwave detector) In Japan and EC-DC Control. MOEs(Measure of effectiveness) are car numbers in the dilemma zone , max-out probability in the safe side and the average stopping delay in the progress side. We choose a signalized intersection in rural highway to analyze the effect of the strategies and practiced an on-the-spot survey. The result of the survey is applied to the basic data in the simulator. Consequently, strategy of the dilemma zone control using R-detector(microwave detector) in Japan is the best effective in the safe side and EC-DC control is the best in the progress side. Based on the result, we developed the effective strategy of the signal control . This strategy is composed of the strategy of Japan and the detector on the stopping line used in the EC-DC control. On the result of the analysis, new strategy is the best effective in two sides.

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Design and Construction of Detector Module for UFFO Burst Alert & Trigger Telescope

  • Jung, Aera;Ahmad, Salleh;Barrillon, Pierre;Brandt, Soren;Budtz-Jorgensen, Carl;Castro-Tirado, Alberto J.;Chen, Pisin;Choi, Ji Nyeong;Choi, Yeon Ju;Connell, Paul;Dagoret-Campagne, Sylvie;Eyles, Christopher;Grossan, Bruce;Huang, Ming-Huey A.;Jeong, Soomin;Kim, Ji Eun;Kim, Min Bin;Kim, Sug-Whan;Kim, Ye Won;Krasnov, A.S.;Lee, Jik;Lim, Heuijin;Linder, Eric V.;Liu, T.C.;Lund, Niels;Min, Kyung Wook;Na, Go Woon;Nam, Ji Woo;Panasyuk, Mikhile I.;Park, Il Hung;Ripa, Jakub.;Reglero, Victor;Rodrigo, Juana M.;Smoot, George.F.;Suh, Jung Eun;Svertilov, Sergei.;Vedenkin, Nikolay;Wang, Min-Zu;Yashin, Ivan
    • The Bulletin of The Korean Astronomical Society
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    • v.37 no.2
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    • pp.207.1-207.1
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    • 2012
  • One of the key aspects of the upcoming Ultra-Fast Observatory (UFFO) Pathfinder for Gamma-Ray Bursts(GRBs) identification will be the UFFO Burst Alert & Trigger Telescope(UBAT), based on a novel space telescope technique. The UBAT consists of coded mask, hopper, and detector module(DM). The UBAT DM consists of YSO crystal arrays, multi-anode photo mulipliers, and readout electronics. We will present the design and construction of the UBAT DM, and the response of the UBAT DM to X-ray sources.

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The Effect of Mg Precursors on Optical and Structural Characteristics of Sol-Gel Processed Mg0.3Zn0.7O Thin Films (졸-겔법으로 성장시킨 Mg0.3Zn0.7O 박막의 Mg 전구체의 종류에 따른 광학적·구조적 특성에 관한 연구)

  • Yeom, Ahram;Kim, Hong Seung;Jang, Nak Won;Yun, Young;Ahn, Hyung Soo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.33 no.3
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    • pp.214-218
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    • 2020
  • In this study, MgxZn1-xO thin films, which can be applied not only to active layers of light-emitting devices (LEDs), such as UV-LEDs, but also to solar cells, high mobility field-effect transistors, and power semiconductor devices, are fabricated using the sol-gel method. ZnO and Mg0.3Zn0.7O solution synthesized by the sol-gel method and the thin film were grown by spin coating on a Si (100) substrate and sapphire substrate. The solutions are synthesized by dissolving precursor materials in 2-methoxyethanol (2-ME) solvent, and then monoethanolamine (MEA) was added to the mixed solution as a sol stabilizer. Zinc acetate dihydrate is used as a ZnO precursor, while Mg nitrate hexahydrate and Mg acetate tetrahydrate are used as an MgO precursor. Then, the optical and structural characteristics of the fabricated thin films are compared. The molar concentration of the Zn precursor in the solvent is fixed at 0.3 M, and the amount of the Mg precursor is 30% of Mg2+/Zn2+. The optical characteristics are measured using an UV-vis spectrophotometer, and the transmittance of each wavelength is measured. Structural characteristics are measured using X-ray diffraction (XRD) and transmission electron microscopy (TEM). Composition analyses are performed using energy dispersive X-ray spectroscopy (EDS). The Mg0.3Zn0.7O thin film was well formed at the ratio of the Mg precursor added regardless of the type of Mg precursor, and the c-axis of the thin film was decreased, while the band gap was increased to 3.56 eV.

A Study on Genetic Analysis and Extract Cytotoxicity of Scolopendra subspinipes multilans L. Koch (노랑머리왕지네의 유전학적(遺傳學的) 분석(分析) 및 약침액(藥鍼液)의 세포독성(細胞毒性)에 관한 연구(硏究))

  • Kim, Sung-Nam;Lim, Jeong-A;Lee, Sung-Yong;Hwang, Woo-Jun;Lee, Geon-Mok;Cho, Nam-Geun;Seo, Jung-Chul;Moon, Hyung-Cheol;Kim, Sung-Chul
    • Journal of Pharmacopuncture
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    • v.9 no.2
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    • pp.49-65
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    • 2006
  • Objective : The purpose of this study is to investigate nucleotide sequence and extract cytotoxicity of Scolopendrae corpus. The nature and taste of Scolopendrae corpus is hot, Warm and toxic, and the effect of this is dispelling wind, anti-spasmodic action and detoxication so it has been used for C.V.A, facial palsy, sensory disorder at extremities, wounds and arthritis. Methods : Scolopendrae corpus were collected by locality on the market. They were morphologically classified. Their nucleotide sequence was investigated and compared among them. In addition, the water-alcohol extract cytotoxicity of them was studied by MTT-based cytotoxicity assay. Results : It was shown that the each Scolopendrae corpus by locality is almost identical at genetic result and is identified as Scolopendra subspinipes mutilans L. Koch. Nucleotide sequence of Scolopendra subspinipes mutilans L. Koch in this study will help to discriminate other species of Scolopendrae corpus. The water-alcohol extract of Scolopendra subspinipes mutilans L. Koch did not induce cytotoxicity on Hep G2, L929 cell and peritoneal macrophages. Besides, it did not influence nitrite production of peritoneal macrophages. These results can be used as basic data for genetic discrimination with another species of scolopendrae corpus.

THE EFFECT OF ACID ETCHING ON GLASS IONOMER CEMENT SURFACES (Glass ionomer cement 표면의 산부식 효과에 관한 연구)

  • Han, Seung-Weon;Park, Sang-Jin;Min, Byung-Soon;Choi, Ho-Young;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.18 no.1
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    • pp.1-26
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    • 1993
  • The purpose of this study was to investigate the effect of acid etching on the surface appearance and fracture toughness of five glass ionomer cements. Five kinds of commercially available glass ionomer cements including chemical curing filling type, chemical curing lining type, chemical curing metal reinforced type, light curing tilling type and light curing lining type were used for this study. The specimens for SEM study were fabricated by treating each glass ionomer cement with either visible light curing or self curing after being inserted into a rubber mold (diameter 4mm, depth 1mm). Some of the specimens were etched with 37% phosphoric acid for 0, 15, 30, 60, go seconds, at 5 minutes, 1 hour and 1 day after mixing of powder and liquid. Unetched ones comprised the control group and the others were the experimental groups. The surface texture was examined by using scanning electron microscope at 20 kV. (S-2300, Hitachi Co., Japan). The specimens for fracture toughness were fabricated by curing of each glass ionomer cement previously inserted into a metal mold for the single edge notch specimen according to the ASTME399. They were subjected to a three-point bend test after etching for 0, 30, 60, and 90 seconds at 5 minutes-, 1 hour-and 1 day-lapse after the fabrication of the specimens. The plane strain fracture toughness ($K_{IC}$) was determined by three-point bend test which was conducted with cross-head speed of 0.5 mm/min using Instron universal testing machine (Model No. 1122) following seven days storage of the etched specimens under $37^{\circ}C$, 100% humidity condition. Following conclusions were drawn. 1. In unetched control group, crack was present, but the surface was generally smooth. 2. Deterioration of the surface appearance such as serious dissolving of gel matrix and loss of glass particles occured as the etching time was increased beyond 15 s following Immediate etching of chemical curing type of glass ionomer cements. 3. Etching after 1 h, and 1 d reduced surface damage, 15 s, and 30s etch gave rough surface appearance without loss of glass particle of chemical curing type of glass ionomer cements. 4. Light curing type glass ionomer cement was etched by acid, but there was no difference in surface appearances according to various waiting periods. 5. It was found that the value of plane stram fracture toughness of glass ionomer cements was highest in the light curing filling type as $1.79\;MNm^{-1.5}$ followed by the light curing lining type, chemical curing metal reinforced type, chemical curing filling type and chemical curing lining type. 6. The value of plane stram fracture toughness of the chemical curing lining type glass ionomer cement etched after 5 minutes was lower than those of the cement etched after 1 hour or day or unetched (P < 0.05). 7. Light curing glass ionomer cement showed Irregular fractured surface and chemical curing cement showed smooth fractured surface.

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Fabrication and Characterization of Lead Oxide (PbO) Film for High Efficiency X-ray Detector (고효율 X선 검출기 적용을 위한 PbO 필름 제작 및 특성 연구)

  • Cho, Sung-Ho;Kang, Sang-Sik;Choi, Chi-Won;Kwun, Chul;Nam, Sang-Hee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.06a
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    • pp.329-329
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    • 2007
  • Photoconductive poly crystalline lead oxide coated on amorphous thin film transistor (TFT) arrays is the best candidate for direct digital x-ray detector for medical imaging. Thicker films with lessening density often show lower x-ray induced charge generation and collection becomes less efficient. In this work, we present a new methodology used for the high density deposition of PbO. We investigate the structural properties of the films using X-ray diffraction and electron microscopy experiments. The film coatings of approximately $200\;{\mu}m$ thickness were deposited on $2"{\times}2"$ conductive-coated glass substrates for measurements of dark current and x-ray sensitivity. The lead oxide (PbO) films of $200\;{\mu}m$ thickness were deposited on glass substrates using a wet coating process in room temperature. The influence of post-deposition annealing on the characteristics of the lead oxide films was investigated in detail. X-ray diffraction and scanning electron microscopy, and atomic force microscopy have been employed to obtain information on the morphology and crystallization of the films. Also we measured dark current, x-ray sensitivity and linearity for investigation of the electrical characteristics of films. It was found that the annealing conditions strongly affect the electrical properties of the films. The x-ray induced output charges of films annealed in oxygen gas increases dramatically with increasing annealing temperatures up to $500^{\circ}C$ but then drops for higher temperature anneals. Consequently, the more we increase the annealing temperatures, the better density and film quality of the lead oxide. Analysis of this data suggests that incorporation and decomposition reactions of oxygen can be controlled to change the detection properties of the lead oxide film significantly. Post-deposition thermal annealing is also used for densely film. The PbO films that are grown by new methodology exhibit good morphology of high density structure and provide less than $10\;pA/mm^2$ dark currents as they show saturation in gain (at approximate fields of $4\;V/{\mu}m$). The ability to operate at low voltage gives adequate dark currents for most applications and allows voltage electronics designs.

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Endothelial-specific deletion of Ets-1 attenuates Angiotensin II-induced cardiac fibrosis via suppression of endothelial-to-mesenchymal transition

  • Xu, Lian;Fu, Mengxia;Chen, Dongrui;Han, Weiqing;Ostrowski, Michael C.;Grossfeld, Paul;Gao, Pingjin;Ye, Maoqing
    • BMB Reports
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    • v.52 no.10
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    • pp.595-600
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    • 2019
  • Cardiac fibrosis is a common feature in chronic hypertension patients with advanced heart failure, and endothelial-to-mesenchymal transition (EndMT) is known to promote Angiotensin II (Ang II)-mediated cardiac fibrosis. Previous studies have suggested a potential role for the transcription factor, ETS-1, in Ang II-mediated cardiac remodeling, however the mechanism are not well defined. In this study, we found that mice with endothelial Ets-1 deletion showed reduced cardiac fibrosis and hypertrophy following Ang II infusion. The reduced cardiac fibrosis was accompanied by decreased expression of fibrotic matrix genes, reduced EndMT with decreased Snail, Slug, Twist, and ZEB1 expression, as well as reduced cardiac hypertrophy and expression of hypertrophy-associated genes was observed. In vitro studies using cultured H5V cells further confirmed that ETS-1 knockdown inhibited $TGF-{\beta}1$-induced EndMT. This study revealed that deletion of endothelial Ets-1 attenuated Ang II-induced cardiac fibrosis via inhibition of EndMT, indicating an important ETS-1 function in mediating EndMT. Inhibition of ETS-1 could be a potential therapeutic strategy for treatment of heart failure secondary to chronic hypertension.