• Title/Summary/Keyword: C.M.R.T.

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Development of a Sequence Characteristic Amplified Region Marker linked to the L4 Locus Conferring Broad Spectrum Resistance to Tobamoviruses in Pepper Plants

  • Kim, Hyun Jung;Han, Jung-Heon;Yoo, Jae Hyoung;Cho, Hwa Jin;Kim, Byung-Dong
    • Molecules and Cells
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    • v.25 no.2
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    • pp.205-210
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    • 2008
  • To develop molecular markers linked to the $L^4$ locus conferring resistance to tobamovirus pathotypes in pepper plants, we performed AFLP with 512 primer combinations for susceptible (S pool) and resistant (R pool) DNA bulks against pathotype 1.2 of pepper mild mottle virus. Each bulk was made by pooling the DNA of five homozygous individuals from a T10 population, which was a near-isogenic $BC_4F_2$ generation for the $L^4$ locus. A total of 19 primer pairs produced scorable bands in the R pool. Further screening with these primer pairs was done on DNA bulks from T102, a $BC_{10}F_2$ derived from T10 by back crossing. Three AFLP markers were finally selected and designated L4-a, L4-b and L4-c. L4-a and L4-c each underwent one recombination event, whereas no recombination for L4-b was seen in 20 individuals of each DNA bulk. Linkage analysis of these markers in 112 $F_2$ T102 individuals showed that they were each within 2.5 cM of the $L^4$ locus. L4-b was successfully converted into a simple 340-bp SCAR marker, designated L4SC340, which mapped 1.8 cM from the $L^4$ locus in T102 and 0.9 cM in another $BC_{10}F_2$ population, T101. We believe that this newly characterized marker will improve selection of tobamovirus resistance in pepper plants by reducing breeding cost and time.

Cu/Si/Cu Ohmic contacts to n-type 4H-SiC (n형 4H-SiC의 Cu/Si/Cu 오옴성 접합)

  • 정경화;조남인;김민철
    • Proceedings of the International Microelectronics And Packaging Society Conference
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    • 2002.11a
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    • pp.73-77
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    • 2002
  • Characteristics of Cu/Si/Cu ohmic contacts to n-type 4H-SiC were investigated systematically. The ohmic contacts were formed by rf sputtering of multi layer Cu/Si/Cu sputtered sequentially. The annealings were peformed With 2-Step using RTP in vacuum ambient. The specific contact resistivity($\rho$c), sheet resistance(Rs), contact resistance(Rc), transfer length(L$_{T}$) were calculated from resistance(R$_{T}$) versus contact spacing(d) measurements obtained from TLM(transmission line method) structure. Best results were obtained for a sample annealed at vacuum as $\rho$c = 1.0x10$^{-6}$ $\Omega$$\textrm{cm}^2$, Rc = 2$\Omega$ and L$_{T}$ = 1${\mu}{\textrm}{m}$. The physical properties of contacts were examined using XRO and AES. The results showed that copper silicide was formed on SiC and Cu was migrated into SiC.o SiC.

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Selection of Optimum Species of Tetraselmis for Mass Culture (대량배양에 적합한 Tetraselmis종의 선택)

  • 김철원;허성범
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.231-240
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    • 1998
  • Tetraselmis is widely used as a live food because of its easy handling, high nutrient, large size and wide tolerant range of temperature and salinity. In order to find the optimum Tetraselmis species for mass culture in Korea, five species of this microalgae were examined on size, optimum culture condition ${\textperthousand}$s, $^{\circ}C.$) and nutrient composition. The results obtained were as follows: Among five species of Tetraselmis, T. sp.(Haeundae) was the largest(major axis $17.6{\pm}1.87^{\mu}$m, mean cell volume 727${\mu}$m), and T. sp. (China) the smallest (major axis $14.6{\pm}1.46^{\mu}$m, mean cell volume 625m). Tetraselmis was very eurythermal and euryhaline species. But optimum temperature and salinity for growth were 24~$30^{\circ}C.$ and 27~30${\textperthousand}$, respectively. Among five species of Tetraselmis, T. sp. (China) seemed to be the most tolerant of high temperature over $30^{\circ}C.$, and T. tetrathele of low temperature below $6^{\circ}C.$. In culture density, T. suecica showed the highest growth rate among the among the five species. The cell density of this microalgae attained to $141{\times}10^4$cells/ml at $24^{\circ}C.$ and 30${\textperthousand}$ within 7 days. In chemical composition, crude protein amount was the highest in T. suecica (44.50%), and crude lipid amount it T. sp. (Haeundae, 7.13%). Total essential amino acid amount was the highest in T. sp. (Haeundae, 50.4%) and total polyunsaturated amount in T. sp. (China, 11.7%) The results on growth and chemical composition of five species of Tetraselmis indicated that T. suecica seemed to be the most suitable species for mass culture in Korea.

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Effect of HTT on Bending and Tensile Properties of 2D C/C Composites

  • Dhakate, S.R.;Aoki, T.;Ogasawara, T.
    • Carbon letters
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    • v.6 no.4
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    • pp.234-242
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    • 2005
  • Bending and tensile properties of 2D cross-ply C/C composites with processing heat treatment temperature (HTT) are evaluated. C/C composites used are made from two types of PAN based T700 and M40 carbon fibers with phenolic resin as carbon matrix precursor. Both the types of composites are heat treated at different temperatures (ranging from 750 to $2800^{\circ}C$) and characterized for bending and tensile properties. It is observed that, real density and open porosity increases with HTT, however, bulk density does show remarkable change. The real density and open porosity are higher in case T-700 carbon fiber composites at $2800^{\circ}C$, even though the density of M40 carbon fiber is higher. Bending strength is considerably greater than tensile strength through out the processing HTT due to the different mode of fracture. The bending and tensile strength decreases in both composites on $1000^{\circ}C$ which attributed to decrease in bulk density, thereafter with increase in HTT, bending and tensile strength increases. The maximum strength is in T700 fiber based composites at HTT $1500^{\circ}C$ and in M40 fiber based composites at HTT $2500^{\circ}C$. After attending the maximum value of strength in both types of composite at deflection HTT, after that strength decreases continuously. Decrease in strength is due to the degradation of fiber properties and in-situ fiber damages in the composite. The maximum carbon fiber strength realization in C/C composites is possible at a temperature that is same of fiber HTT. It has been found first time that the bending strength more or less 1.55 times higher in T700 fiber composites and in M40 fiber composites bending strength is 1.2 times higher than that of tensile strength of C/C composites.

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cDNAs encoding the antigenic proteins in pathogenic strain of Entamoeba histolytica (이질아메바 병원성 분리주에서 발현되는 항원 단백질을 coding하는 cDNA)

  • 임경일;최종태
    • Parasites, Hosts and Diseases
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    • v.35 no.3
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    • pp.203-210
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    • 1997
  • The difrrrenlial display reverse transcription polymerase chain reaction (DDRT-PCR) aniilysis roils performed to identify the pathogellir strain specific amplicons. mRNAs were purified from the trophozoites of the pathogenif strain YS-27 and the non-pathogenic strain S 16. respectively. Three kinds of rirsl stranded rDNAs were reverse transcribed from the mRNAs by one base anchored oligo-dT 11M (M: A. C, or G) primers. Each cDNA lemplatr was used for DDRT-PCK analysis. A total of 144 pathogenic strain specific amplicons was observed in DDRT-PCR analysis using primer combinations of the 11 arbitrary primers and the 3 one base anchored oli해-dT11M primers. Of these 31 amplit'tons were verified as the amplirons amplified only from the mRNAs of the pathogenic strain by DNA slots biol llybridizatioil. Furthel cklaracleization of the 31 pathogenic strain sprcifil amplicons by DNA slot blot hybridlnation analysis using biotin labeled Probes or the PCR amplified DNA of rysteine proteinase genes revealed that 21 of them were amplliried from the maNAs of the cysteine proteinase genes. Four randomly selected amplirons out of the rest 10 amplirons were used fur screening of cDNA library followed by immunoscreening and all of them were turned outs to be amplified from the mRNA.

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The Relationships among Trunk Control Ability, Dynamic Balance and Gait in Stroke Patients (뇌졸중 환자의 체간조절 능력과 동적균형 및 보행과의 상관관계)

  • Chung, Eun-Jeong;Lee, Jong-Soo;Kim, Seong-Sik;Lee, Byoung-Hee
    • The Journal of Korean Medicine
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    • v.33 no.1
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    • pp.148-159
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    • 2012
  • Objectives: This study investigated the correlation among Postural Assessment Scale for Stroke (PASS), Timed "Up and Go" Test (TUG) and gait (velocity, cadence, step-length, stride-length and single-limb support). Methods: The 70 subjects were assessed on trunk control measured with the use of the PASS, dynamic balance (TUG) and gait function (by GAITRite). The data were analyzed using Pearson product correlation. Results: The PASS total scores were significantly correlated with PASS-M, PASS-C, and PASS-T (r =.80 ~ .88 p<.01). All items of the PASS were significantly correlated with TUG (r = -.63 ~ -.81 p<.01), velocity (r = .44~.58 p<.01), cadence (r =.38 ~.51. p<.01), affected side step length (r = .44 ~.56 p<.01) and affected side stride length (r = .45 ~.59 p<.01). But affected side single-limb support was lowly correlated with PASS-M, PASS-C, PASS-T and PASS-total (r = .25~.36 p<.05). Conclusions: Measures of trunk control were significantly related with values of dynamic balance and gait. Based on these results, trunk control is an essential core component of balance and gait. Trunk control training programs after stroke should be developed and emphasized.

Development of an HVAC&R Systems Energy Evaluation Methodology and Simulation Program for Office Buildings (사무소 건물의 HVAC&R 시스템 에너지 분석방법 및 예측 프로그램 개발)

  • Cho, Jinkyun;Shin, Seungho;Kim, Jonghurn;Hwang, Dongkon;Hong, Hiki
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.25 no.7
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    • pp.363-370
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    • 2013
  • HVAC&R systems are the most energy consuming building services, representing approximately half of the final energy use in the building sector. Despite their significant energy use, there is a lack of a consistent and homogeneous framework to efficiently guide research. This research is about the energy consumptions of HVAC&R systems, with the aim of establishing a common idea for the analysis of building energy efficiency. Our approach deals with the concept of an HVAC&R set that is composed of subsystems. A matrix combination evaluation is examined, and a total of 960 sets can be implemented in a large scale office building. A method as a tool for energy evaluations of HVAC&R systems, and HEET as a energy simulation program, were developed.

Non-Dimensional Analysis on the Ice-on-coil as a Static Ice Making Type (정적제빙형인 관외 제빙에 관한 무차원 정리)

  • Yu, Jik-Su;Kim, Myoung-Jun;Kim, Sung-Yong;Jang, Hyeon
    • Proceedings of the Korean Society of Marine Engineers Conference
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    • 2011.06a
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    • pp.322-322
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    • 2011
  • 본 연구는 동관의 관외착빙형 특성을 조사하기 위한 기초적인 연구로써, 실험의 파라메터와 범위는 브라인의 온도는 -10[$^{\circ}C$], -8[$^{\circ}C$], -6[$^{\circ}C$], 브라인의 유속은1.0[m/s], 1.4[m/s], 1.8[m/s] 그리고물의 초기온도는6[$^{\circ}C$], 9[$^{\circ}C$], 12[$^{\circ}C$] 등으로 설정하였다. 본 논문에서는 무차원 얼음 두께와 무차원 동결 시간의 관계에 대하여 실험적으로 정리 하였다. 브라인온도, 물의 온도, 브라인 유속, 물의 초기온도, 동관의 직경사이즈에 대한 무차원 상관 방정식은 다음과 같이 얻어진다. r+3 = $0.063{\bullet}(t+)+0.206$ [적용범위: R+T+=2.1~4.7].

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