Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Selection of Optimum Species of Tetraselmis for Mass Culture

대량배양에 적합한 Tetraselmis종의 선택

  • Published : 1998.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

Tetraselmis is widely used as a live food because of its easy handling, high nutrient, large size and wide tolerant range of temperature and salinity. In order to find the optimum Tetraselmis species for mass culture in Korea, five species of this microalgae were examined on size, optimum culture condition ${\textperthousand}$s, $^{\circ}C.$) and nutrient composition. The results obtained were as follows: Among five species of Tetraselmis, T. sp.(Haeundae) was the largest(major axis $17.6{\pm}1.87^{\mu}$m, mean cell volume 727${\mu}$m), and T. sp. (China) the smallest (major axis $14.6{\pm}1.46^{\mu}$m, mean cell volume 625m). Tetraselmis was very eurythermal and euryhaline species. But optimum temperature and salinity for growth were 24~$30^{\circ}C.$ and 27~30${\textperthousand}$, respectively. Among five species of Tetraselmis, T. sp. (China) seemed to be the most tolerant of high temperature over $30^{\circ}C.$, and T. tetrathele of low temperature below $6^{\circ}C.$. In culture density, T. suecica showed the highest growth rate among the among the five species. The cell density of this microalgae attained to $141{\times}10^4$cells/ml at $24^{\circ}C.$ and 30${\textperthousand}$ within 7 days. In chemical composition, crude protein amount was the highest in T. suecica (44.50%), and crude lipid amount it T. sp. (Haeundae, 7.13%). Total essential amino acid amount was the highest in T. sp. (Haeundae, 50.4%) and total polyunsaturated amount in T. sp. (China, 11.7%) The results on growth and chemical composition of five species of Tetraselmis indicated that T. suecica seemed to be the most suitable species for mass culture in Korea.

온도와 염분에 대하여 내성이 강하고 대량배양이 쉬워서 조개류의 인공종묘생산시 먹이로서 많이 사용하고 있는 5종의 Tetraselmis (T. tetrathele, T. suecica, T. subcordiformis, T. sp. (Haeundae), T. sp (China)) 중 우리나라 기후 환경에 대향배양이 가장 적합한 종을 선택하기 위핵서 최적 배양환경 (온도, 염분)을 조사하고 이때의 세포크기와 영양성분을 조사하였다. 5종의 Tetraselmis의 세포크기 및 체적은 T. sp. (Haeundea)가 장경 $17.6{\pm}1.87$${\mu}$m, 체적 727${\mu}$$m^3$로 가장 대형종이었으며 중국산 T. sp.가 장경14.6+- 1.46${\mu}$m, 체적 625${\mu}$$m^3$으로 가장 소형종이였다. Tetraselmis는 광염성, 광온성이나 최적성장을 위한 수온과 염분 구간은 각각 24~$30^{\circ}C.$와 27~30%.였다. 5종의 Tetraselmis에서 고수온에 대한 내성은 중국산 Tetraselmis, 저수온데 대한 내성은 T. tetrathele가 높은 것으로 나타났다. 5종의 Tetraselmis중 배양밀도가 가장 높았던 종은 T. suecica였으며, 이 종은 배양 7일째 $24^{\circ}C.$, 30%.에서 세포수가 $141{\times}10^4$cells/ml (s.g.r., 0.3906)로 성장이 가장 좋았다. 5종의 Tetraselmis의 화학성분중 조단백질 함량은 T. suecica가 44.50%로 가장 높게 나타났고 조지방은 해운대산 T. sp.가 7.13%로 높게 나타났다. 필수아미노산은 해운대산 .T.sp.에서 50.40%로 가장 높게 나타났고, 불포화지방산은 중국산 T.sp.에서 11.7%로 가장 높게 나타났다. 5종의 Tetraselmis의 성장과 영양성분 결과로 보아 우리나라에서 대량배양이 가장 적합한 종은 T. seucica임을 알수 있었다.

Keywords

References

  1. J. Fish. Res. Bd. Canada v.25 no.8 Marine phytoplankter fatty acids Ackman, R. G.;C. S. Tocher;J. Mclachlan
  2. J. Phycol. v.21 Chemical profile of seletion species of microalgae with emphasis on lipids Ben-Amotz, A.;T. G. Tornabene;W. H. Thonas
  3. J. Exp. Mar. Biol. Ecol. v.50 The effect of continuous light and light intensity on the reproduction rates of twenty-two species of marine phytoplankton Brank, L. E.;R. R. L. Guillard
  4. J. Exp. Mar. Biol. Ecol. v.145 The amino-acid and sugar composition of 16 species of microalgae used in mariculture Brown, M. R.
  5. Aquaculture v.161 Biochemical composition of microalgae from the green algal classes Chlorophyceae and Prasinophyceae. 1. Amino acids, sugars and pigments Brown, M. R.;S. W. Jeffery
  6. Shuichan yu Jiaoyu. v.1978 no.2 Preliminary report on the culture and effect of Dicrateria zhanjiangensis Hu sp. nov. for the breeding Chen, M. Y.;S. Y. He;C. X. Chen;G. X. Dai
  7. J. Exp. Mar. Biol. Ecol. v.96 Evalution of phytoplankton as diets for juvenile Ostera edulis L. Enright, C. T.;G. F. Newkirk;J. S. Craigie;J. D. Castell
  8. Aquaculture v.18 Growth in bibalve molluscs: nutritional effects of two or more species of algae in diets fed to the American oyster, Crassostrea virginica (Gmelin) and the hard clam, Mercenaria marcenaria (L.) Epifanio, C. E.
  9. Aquaculture v.42 Growth of the marine microalgae Teraselmis suecica in batch culture with different salinities and nutrient concentration Fabregas, J. J.;C. Abalade;B. Herrero, Cabezas;M. Veiga
  10. Aquaculture v.50 Growth, chlorophyll and protein of the martne microalgae Isochrysis galbana in batch cultures with different salinities and high nutrient concentrations Fabregas, J.;C. Herrero;J. Abalde;B. Cabezas
  11. Aquaculture v.83 Biochemical production and variation in the Biochemical profile (total protein, carbohydrates, RAN, lipids and fatty acids) of seven soecies of marine microalgae Fernadez-Reiriz, M. J.;A. Perez-Camacho;M. J. Ferreiro;J. Blanco;M. Planas;M. J. Compos;U. Labrata
  12. Gram. Can. J. Microbiol. v.8 Studies for marine planktonic diatoms.Ⅰ . Cyclotella nana Hustedt and Detomnule conferracea (Cleve) Guillard, R. L.;J. H. Ryther
  13. J. Mar. Biol. Assoc. U. K. v.53 Growth measurement. In Hand book of physiocological method Guillard, R. L.
  14. J. Applied Phycol. v.2 Effects of nutrient and light limitation on the biochemical composition of phytoplankton Harrison, P. J.;P. A. Thompson;G. S. Calderwood
  15. J. Mar. Biol. Assoc. U. K. v.53 The effect of supplementary algal feeding of hatchery breeding stock of Ostrea edulis L. on larval vigor Helm, M. M.;D. L. Holland;R. R. Stephenson
  16. Fish. Res. Bd. Canada. v.21 no.4 The cell division rates of some marine phytoplankton as a function of light and temperature Jitts, H. R.;C. D. McAllister;K. Stephens;J. D. H. Strickland
  17. Aquaculture v.21 The influence of salinity on the production of two commercially important unicellular marine algae Laing, I.;S. D. Utting
  18. Aquaculture v.22 Factors affecting the semi-continuous production of Tetraselmis suecica (Kilin) Buth. in 200-1 vessels Laing, I.;M. M. Helm
  19. J. Mar. Biol. Ass. U.K. v.61 The effect of algal and artificial diets on the growth and fatty acid composition of Crassostrea gigas spat Langdon, C. J.;M. J. Waldock
  20. Oceanogr. v.9 Some interaction of temperature, light intensity, and nutrient concentration during the continuous culture of Nitzschia closterium and Tetraselmis sp. Simnol. Maddux, W. S.;F. J. Raymond
  21. Can. J. Microbiol. v.7 Effect of salinity on growth and chlorophyll content in representative classes of unicellular marine algae Mclachlan, J.
  22. J. phycol. v.26 Influence of irradiance on the fatty acid composition of phytoplankton Thompson, P. A.;P. J. Harrison;J. N. C. Whyte
  23. Biol. Bull. v.120 The effect of temperature on the growth and survival of several marine algal species Ukeles, S. R.
  24. Aquaculture Engineering v.4 Influence of nitrogen availability on the biochemical composition of three unicellular marine algae of commercial importance Utting, S. D.
  25. Proceedings of the Second International Conference of Aquaculture Nutrition : Biochemical and Physiological Approaches to Shellfish Nutrition Phytoplankton as a food source for bivalve larvae Webb, K. L.;F. L. Chu;G. pruder(ed.);C. J. Langdin(ed.);D. E. Conkilin(ed.)
  26. Aquaculture v.60 Biochemical composition and energy content of six species of phytoplankton used in mariculture of bivalves Whyte, J, N, C
  27. Aquaculture v.14 テラトセルミスTetraselmis tetrathele の 大量培養法と餌料價値 岡內正典
  28. 부산수산대학교 석사학위 논문 실내사육에서의 가리비(Patinopecten yessoensis) 치패의 먹이효과 金惠英
  29. 부산수산대학교 석사학위 논문 식물성 먹이생물 Pavolva lutheri의 성장에 미치는 조도와 온도의 영향 朴斗元
  30. 부산수산대학교 석사학위 논문 식물성 먹이생물 4종의 최적배양환경 朴正銀
  31. 한국양식학회지 v.8 no.4 해산 규조류 7종의 먹이효과 비교 裵晋晞