• Korean Journal of Medicinal Crop Science
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• v.12 no.2
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• pp.135-140
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• 2004

Total RNAs were isolated from cultured roots of Scopolia parviflora, $poly(A)^+$ RNA was obtained through the mRNA purification, cDNA library of Hnh6h was constructed. Recombinant baculoviruses in Spodoptera frugiperda (Sf) cells were constructed by use of the transfer vector pBacPAK, which has the AcNPV sequence under the polyhedrin promoter. The expression vector carrying Hnh6h gene was transferred to S. parviflora and obtained transgenic hairy root lines. Our results confirmed the over expression of the H6H protein was used by anti-pBacPAK about cDNAs of S. parviflora. This study will served for production of tropane alkaloids by metabolic engineering.

• Korean Journal of Plant Taxonomy
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• v.45 no.2
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• pp.136-144
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• 2015

We reviewed the taxonomic identity and morphological characteristics of Cardamine manshurica (Kom.) Nakai based on its original description and type specimens. Cardamine manshurica, with its smaller flowers and siliques and earlier flowering season, is distinguished from the closely related species C. flexuosa With. This species-level difference is additionally supported in a phylogenetic tree constructed using nrDNA ITS sequences. Taken together, we therefore propose that C. manshurica is a distinct species. The scientific name 'Cardamine manshurica (Kom.) Nakai' is accepted.

• Journal of Plant Biology
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• v.29 no.1
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• pp.53-65
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• 1986

The montane forests of Ulreung Island, Korea, were investigated by the ZM school method. By comparing the montane forests of this island with those of Korean Peninsula and of Japan, a new order, F a g e t a l i a m u l t i n e r v i s, a new alliance, F a l g i o n m u l t i n e r v i s, a new association, H e p a t i c o-F a g e t u m m u l t i n e r v i s and Rhododendron brachycarpum-Pinus parviflora community were recognized. The H e p a t i c o - F a g e t u m m u l t i n e r v i s was further subdivided into four subassociations; Subass. of Sasa kurilensis, Subass. of Rumohra standishii, Subass. of Rhododendron brachycarpum and Subass. of typicum. Each community was described in terms of floristic, structural and environmental features.

• Korean Journal of Medicinal Crop Science
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• v.11 no.5
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• pp.358-363
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• 2003

ScopoIamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia. parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supernatant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.209-215
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• 2006

We investigated antioxidant capacity in leaves of 14 coniferous trees under field conditions. We focused on understanding the species characteristics on antioxidant systems and screening the coniferous tree species with the best antioxidant systems using their characteristics. The antioxidant capacity of 14 coniferous trees was divided into three groups. First group was Thuja orientalis and Chamaecyparis obtusa and those species had the highest content of ${\beta}$-carotene and xanthophyll. Second group, C. obtusa and Juniperus chinensis, used antioxidant enzymes to mitigate stress. C. obtusa represented high activity at superoxide dismutase (SOD), glutathione reductase (GR), and peroxidase (POD), and J. chinensis exhibited high activity at SOD, POD, catalase (CAT). Third group employed antioxidant such as ascorbic acid and ${\alpha}$-tocopherol. The antioxidant content of T. orientalis was the highest while that of Pinus parviflora and C. obtusa were the lowest. Few species belonged in three groups simultaneously, and most species belonged in at least one or two groups. In summary, we proposed that C. obtusa and T. orientalis had the highest antioxidant capacity while P. parviflora and P. desiflora for. multicalus had the lowest antioxidant capacity.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.509-522
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• 2008

한국산 가막사리속(Bidens L.)에 포함된 10분류군(8종 2변종)의 수과형태를 연구하기 위하여 입체현미경과 주사전자현미경을 이용하여 관찰하였다. 연구된 가막사리속에서 까끄라기를 제외한 수과의 크기는 $3.0-19.5{\times}0.7-2.6 mm$이다. 관모는2-4개의 까끄라기로 구성되어 있고, 까끄라기의 길이는 2.2-5.6 mm이다. 강모의 배열은 1-3열이었다. 수과의 다형화가 6개의 분류군(B. bipinnata, B. biternata, B. frondosa, B. parviflora, B. pilosa var. pilosa, B. tripartita var. tripartita)에서 확인되었다. 수과의 형태와 까끄라기의 수에 의해 4가지 유형으로 구분되었다. - Type A: 장타원형이고, 까끄라기 수는 2개(B. parviflora); Type B: 장타원형이고, 까끄라기 수는 3개 이상(B. bipinnata, B. biternata, B. pilosa var. pilosa, B. pilosa var. minor) Type C: 도란형이고, 까끄라기의 수는 2개(B. frondosa, B. radiata var. radiata, B. radiata var. pinnatifida, B. tripartita var. tripartita); Type D: 도란형이고, 까끄라기의 수는 3개 이상(B. cernua). 까끄라기의 강모 표면무늬는 3가지 즉 나선상, 평활상, 불규칙한 직선형으로 나타났다. 수과의 표면은 각피층이 발달한 다각형, 평활상 그리고 굴곡형으로 나타났다. 수과형질의 분류학적 적용에 대하여 간략하게 토의하였다. 수과형질을 바탕으로 한국산 가막사리속 분류군에 대한 검색표를 제시하였다.

• YAKHAK HOEJI
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• v.52 no.3
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• pp.232-239
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• 2008

The aim of this study was to evaluate if a composition of herb extracts, PLX-PLS was effective to treat atopic dermatitis (AD) in mice. SPZZC is a composition of herb extracts containing the roots of Scopolia parviflora and Paeonia lactiflora, the herb of Zizania caudiflora, the fruit of Ziziphus jujuba and the leaf of Chinese arborvitae. AD in BALB/c mouse was induced by patching ovoalbumin on the backside, while it in NC/Nga mouse was induced by repeated application of 1-chloro 2,4-dinitrobenzene (DNCB). Mice were topically treated with SPZZC or Domohorn ointment on the backside for 2 weeks (BALB/c) or 1 week (NC/Nga). Scratching behavior, clinical skin severity and the levels of WBC, neutrophil, eosinophil and total serum IgE were measured. After AD induction, scores of scratching behavior and clinical skin severity and the levels of WBC, neutrophil, eosinophil and total serum IgE were increased. Treatment with SPZZC significantly decreased scores of scratching behavior and clinical skin severity in a dose dependent manner in NC/Nga and BALB/c mice. Treatment with SPZZC 2% significantly decreased also the levels of WBC, neutrophil, eosinophil and total serum IgE. Especially, treatment of SPZZC 2% reduced more rapidly score of clinical skin severity than clobetasol cream. These results suggest that the SPZZC may be an alternative substance for the management of AD.

• Journal of agriculture & life science
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• v.46 no.3
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• pp.19-26
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• 2012

The purpose of this study was to provide basic data for the cultivation of Allium victorialis var. platyphyllum in the area covered with forest vegetation. The populations of A. victorialis var. platyphyllum in Ulleungdo were naturally growing in the communities of Fagus japonica var. multinervis, Acer okamotoanum, Pinus parviflora, Tsuga sieboldii, Pinus densiflora, Pinus thunbergii, Tilia amurensis, and Alnus maximowiczii. On average, the study area of A. victorialis var. platyphyllum population was located in the places of 563 m above sea level, and in the slope area of $27.8^{\circ}$. Soil texture of its inhabitation site mainly consisted of sandy soil, sandy loam, loamy sand, and loam. The depth of the organic layer was 6 cm on average, soil pH 5.4, available phosphate content 18.5 ppm, and cation exchange capacity $18.6cmol_c/kg$. The correlation between the site factors and soil factors by CCA analysis showed that the community of beech tree indicated positive correlation with carbon, nitrogen, sand content, and cation exchange capacity.

• BMB Reports
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• v.39 no.4
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• pp.361-370
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• 2006

Genetic variation and molecular phylogeny of 22 taxa representing 14 extant species and 3 unidentified taxa of Boesenbergia in Thailand and four outgroup species (Cornukaempferia aurantiflora, Hedychium biflorum, Kaempferia parviflora, and Scaphochlamys rubescens) were examined by sequencing of 3 chloroplast (cp) DNA regions (matK, psbA-trnH and petA-psbJ). Low interspecific genetic divergence (0.25-1.74%) were observed in these investigated taxa. The 50% majority-rule consensus tree constructed from combined chloroplast DNA sequences allocated Boesenbergia in this study into 3 different groups. Using psbA-1F/psbA-3R primers, an insertion of 491 bp was observed in B. petiolata. Restriction analysis of the amplicon (380-410 bp) from the remaining species with Rsa I further differentiated Boesenbergia to 2 groupings; I (B. basispicata, B. longiflora, B. longipes, B. plicata, B. pulcherrima, B. tenuispicata, B. thorelii, B. xiphostachya, Boesenbergia sp.1 and Boesenbergia sp.3; phylogenetic clade A) that possesses a Rsa I restriction site and II (B. curtisii, B. regalis, B. rotunda and Boesenbergia sp.2; phylogenetic clade B and B. siamensis; phylogenetic clade C) that lacks a restriction site of Rsa I. Single nucleotide polymorphism (SNP) and indels found can be unambiguously applied to authenticate specie-origin of all investigated samples and revealed that Boesenbergia sp.1, Boesenbergia sp.2 and B. pulcherrima (Mahidol University, Kanchanaburi), B. cf. pulcherrima1 (Prachuap Khiri Khan) and B. cf. pulcherrima2 (Thong Pha Phum, Kanchanaburi) are B. plicata, B. rotunda and B. pulcherrima, respectively. In addition, molecular data also suggested that Boesenbergia sp.3 should be further differentiated from B. longiflora and regarded as a newly unidentified Boesenbergia species.

• Journal of Environmental Science International
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• v.21 no.11
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• pp.1307-1320
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• 2012

The purpose of this paper is to provide information on planting construction for healthy plant growth. To achieve this purpose, this study analyzed the planting type, planting density, withering rate, soil characteristics, and cambium electrical resistance (CER) of withered trees in an apartment complex with a high withering rate. The major plant groups examined consisted of native broad-leaved tree species (39.3%), native narrow-leaved tree species (24.2%), and native broad-leaved - exotic narrow-leaved tree species (16.4%). The planting density of the green area, where trees were planted from 0.0 to 0.3 trees per unit area, was measured as 98.4%. Withered trees were found in 19 of the 20 planted species, and the withering rate was 41.8% (610 withered/1,461 planted). Withering rates for tree species were measured as follows: Sophora japonica and Salix babylonica (100.0%), Magmolia denudata (84.3%), Lindera obtusiloba (74.7%), cornus kousa (69.3%), acer triflorum (69.2%), diospyros kaki (66.7%), Prunus yedoensis (62.8%), Acer palmatum (52.6%), Prunus armeniaca (51.1%), Chaenomeles sinensis (43.7%), Ginkgo biloba (40.9%), Zelkova serrata (31.0%), Cornus officinalis (28.6%), Taxus cuspidata (25.6%), Pinus densiflora (21.4%), Pinus parviflora (15.2%), Pinus strobus (14.6%), and Abies holophylla (10.3%). Soil chemical analyses for 18 samples revealed that as the withering rate increased, the following occurred: (a) the ratio of silt and clay in soil increased; (b) the soil pH, organic matter rate, nitrogen, available phosphorus, and cation exchange capacity (CEC) in samples were graded as "inadequate," based on the plant grading evaluation; and (c) the NaCl and cation exchange capacity were evaluated as "somewhat satisfactory." The measurement of CER for withering rate shows electrical resistance for higher withering rate are higher, which could predict that a tree will not grow well.