Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Selection of Optimal Biotic Elicitor on Tropane Alkaloid Production of Hairy Roots in Scopolia parviflora Nakai

미치광이풀 모상근 배양에서 Tropane Alkaloids 생산성 증진을 위한 최적 생물학적 엘리시터 선발

  • Jung, Hee-Young (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Seung-Mi (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Young-Min (Division of Forest Science, Gyeongsang National University) ;
  • Kim, Yong-Duk (Division of Forest Science, Gyeongsang National University) ;
  • Yang, Jae-Kyung (Division of Forest Science, Gyeongsang National University) ;
  • Chung, Young-Gwan (Division of Forest Science, Gyeongsang National University) ;
  • Choi, Myung-Suk (Division of Forest Science, Gyeongsang National University)
  • Published : 2003.12.31
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Abstract

ScopoIamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia. parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supernatant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

미치광이풀의 모상근 뱌양에 의해 의학적으로 중요한 물질인 scopolamine과 hyoscyamine 생산을 증진시키기 위해 3종의 곰팡이와 1종의 효모 유래의 생물학적 elicitor를 supernatant와 homogenate의 형태로 처리하였다. Elicitor 중 Candida albicans는 supernatant와 homogenate 모두 scopolamine 생산성을 증가시켰다. Fusarium solani의 homogenate와 C. albicans의 supernatant 처리로 hyoscyamine 함량이 증가되었다. 그러나 나머지 fungus strain도 대조구에 비해 tropane alkaloids의 생산을 다소 증가시켰다. 본 연구에서는 C. albicans가 tropane alkaloids 생산에 적합한 elicitor임으로 구명되었다. 본 연구 결과는 미치광이풀 모상근 대량배양에 의한 tropane alklaoids 대량생산에 기여할 것이다.

Keywords

References

  1. Collins-Pavao M, Chin CK, Pedersen H (1996) Taxol partitioning in two-phase plant cell cultures of Taxus breviiolia, J. Biotechnol. 49:95-100 https://doi.org/10.1016/0168-1656(96)01525-8
  2. Eilert U, Kurz WGW, Constabel F (1985) Stimulation of sanguarine accumulation in Papaver somniferum cell cultures by fungal elicitors. J. Plant Physiol. 119:65 76 https://doi.org/10.1016/S0176-1617(85)80216-9
  3. Funk C, Gugler K, Brodelins P (1987) Increased secondary product formation in plant cell suspension cultures after treatment with a yeast carbohydrate preparation (elicitor) Phytochern. 26:401-405 https://doi.org/10.1016/S0031-9422(00)81421-1
  4. Hashimoto T, Yun DJ, Yamada Y (1993) Production of tropane alkaloids in genetically engineered root culture. Phytochem. 32:713-718 https://doi.org/10.1016/S0031-9422(00)95159-8
  5. Jung BY, Kang MJ, Kang YM, Yun DJ, Bahk JD, Chung YG, Choi MS (2002) Optimal culture conditions and XAD resin on tropane alkaloid production in Scopolia parviflora hairy root cultures. Korean J. Biotechnol, Bioeng. 17:525-530
  6. Jung BY, Kang SM, Kang YM, Kang MJ, Yun DJ, Bahk JD, Yang lK, Choi MS (2003) Enhanced production of scopolamine by bacterial elicitors in adventitious hairy root cultures of Scopolia parviflora. Enzyme Microb. Tcchnol. 33:987-990 https://doi.org/10.1016/S0141-0229(03)00253-9
  7. Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep. 5:239-242 https://doi.org/10.1007/BF00269811
  8. Kargi F, Rosenberg MZ (1987) Plant cell bioreactors : present states and future trends. Biotechnol. Prog. 3:1-8 https://doi.org/10.1002/btpr.5420030102
  9. Leon J, Rojo E, Sanchez-Serrano JJ (2001) Wound signalling in plants. J. Exp, Bot. 52:1-9 https://doi.org/10.1093/jexbot/52.354.1
  10. Pitta-Alvarez SI, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme and Microb. Technol, 26:252-258 https://doi.org/10.1016/S0141-0229(99)00137-4
  11. Roberts SC, Shuler ML (1997) Large-scale plant cell culture. Curr. Opin. Biotechnol. 8:154-159 https://doi.org/10.1038/nbt0290-154
  12. Wang C, Wu J, Mei Z (2001) Enhancement of taxol production and excretion in Taxus chinen sis cell culture by fungal elicitation and medium renewal. Appl, Microbiol. Biotechnol. 55:404-410 https://doi.org/10.1007/s002530000567
  13. Yamada Y, Tabata M (1997) Plant biotechnology of tropane alkaloids. Plant Biotechnol. 14:1-10
  14. Zabetakis I, Edwards R, Hamilton JIG, O'Hagan D (1998) The biosynthetic relationship between littorine and hyoscyamine in transformed roots of Datura stramonium. Plant Cell Rep. 18:341-345 https://doi.org/10.1007/s002990050583
  15. Zhao J, Zhu WH, Hu Q (2001) Selection of fungal elicitors to increase indole alkaloid accumulation in Catharanthus rose us suspension cell culture. Enzyme Microb. Technol. 28:666-672 https://doi.org/10.1016/S0141-0229(01)00309-X