• Journal of Animal Science and Technology
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• v.49 no.5
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• pp.549-558
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• 2007

C4B and BAT2, assigned to the SLA class III region, were recently reported on relation with human diseases. The primers for RT-PCR and RACE-PCR for CDS analysis of these genes of pig were designed by aligning the CDSs of humans and mice from GenBank. After we amplified and sequenced with these primers and cDNAs, the full-length CDSs of pig were determined. The CDS lengths of C4B and BAT2 were shown as 5226 bp and 6501 bp. In addition, the identities of nucleotide sequences with human and mouse were 76% to 87%, and the identities of amino acids were 72% to 90%. After we carried out the alignment with determined CDSs in this study and pig genomic sequences from GenBank, the primers for cSNP detection in genome were designed in intron regions that flanked one or more exons. Then, we amplified and directly sequenced with genomic DNAs of six pig breeds. Four cSNPs from C4B and three 3 cSNPs from BAT2 were identified. In addition, amino acid substitution occurred in six cSNP positions except for C4248T of C4B. By the Multiplex-ARMS method, we genotyped seven cSNPs with DNA samples used for direct sequencing. We verified that this result was the same as that analyzed using direct sequencing. To demonstrate recrudescence, we performed both direct sequencing and Multiplex-ARMS on two randomly selected DNA samples. The genotype of each sample showed the same result from both methods. Therefore, seven cSNPs were identified from C4B and BAT2 and could be used as the basic data for haplotype analysis of SLA class III region. Moreover, the Multiplex-ARMS method should be powerful for genotyping of genes assigned to the whole SLA region for the xenograft study.

• Korean Journal of Crystallography
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• v.8 no.1
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• pp.1-5
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• 1997

The crystal structure of the title compound consists of discrete 4-N,N-dimethylamino-4'-N'-methyl-stilbazolium, $C_{16}H_{19}N_2$, and tosylate, $C_7H_7SO_3$, dimer. The 4-N,N-dimethylamino-4'-N'-methyl-stilbazolium molecule has a trans conformation at the central C=C double bond: the dihedral angle between the phenyl and the pyridyl rings is $5.7(2)^{\circ}$ and the whole molecule is planar within $0.138(8){\AA}$. Tosylate molecules display hydrogen-bonded dimers with the O-H...O distances of 2.855(9) and $2.899(8){\AA}$, respectively. The shortest intermolecular contact is the distance $3.10(1){\AA}$ between O(3) and C(16).

• BMB Reports
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• v.47 no.10
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• pp.563-568
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• 2014

Human genome projects have enabled whole genome mapping and improved our understanding of the genes in humans. However, many unknown genes remain to be functionally characterized. In this study, we characterized human chromosome 4 open reading frame 34 gene (hC4orf34). hC4orf34 was highly conserved from invertebrate to mammalian cells and ubiquitously expressed in the organs of mice, including the heart and brain. Interestingly, hC4orf34 is a novel ER-resident, type I transmembrane protein. Mutant analysis showed that the transmembrane domain (TMD) of hC4orf34 was involved in ER retention. Overall, our results indicate that hC4orf34 is an ER-resident type I transmembrane protein, and might play a role in ER functions including $Ca^{2+}$ homeostasis and ER stress.

• Journal of Life Science
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• v.8 no.2
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• pp.203-207
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• 1998

NAD4 as a gene encoding NADH dehydrogenase subunit 4 in the micodhondrion from maize has been cloned using RT-PCR and sequenced for examining RNA edited sites. Analysis of mt cDNA sequences showed the typical RNA editing patterns and unusual base changes as well;RNA editing from cDNA sequences occured base change from c to U in most cases, however transitions from t to g and G to A were also observed. Even though those editings appared to be occurred randomly, RNA edited sites showed mostly in exon 1 and exon 4 regions, when compared with NAD4 cDNA from wheat, locations of edited sites did not consistent with each other suggesting that the phenomenon of RNA editing occured randomly not site-specific manner.

• YAKHAK HOEJI
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• v.35 no.1
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• pp.22-29
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• 1991

pMB4 is a 2.4-kilobase plasmid of Staphylococcus aureus TR-1 that confers inducible resistance to the macrolide-lincosamide-streptogramin B(MLS) antibiotics. By subcloning studies, it was found that the MLS resistance determinant was located at 1.0Kb fragment between Sau3AI and TaqI sites. DNA sequence of the MLS resistant determinant, named ermC-4 was determined, and found to be highly homologous with that of ermC. Because the leader peptide sequence of ermC-4 was identical with that of ermC, the expression of the resistance gene is thought to be controlled by posttranscriptional attenuation in S. aureus TR-1.

• Journal of Digestive Cancer Research
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• v.5 no.2
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• pp.73-85
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• 2017

Background: To compare the quality of life (QoL), the convenience of chemotherapy and satisfaction between colon cancer patients treated with FOLFOX4 and XELOX. Methods: The study was conducted in 26 patients with stage III colon cancer. Patients were received FOLFOX4 (n=17) or XELOX (n=9). QoL, convenience, and satisfaction were assessed using the Quality of Life Questionnaire-C30 (QLQ-C30), Quality of Life Questionnaire-Chemotherapy Induced Peripheral neuropathy (QLQ-CIPN) and Functional Assessment of Chronic Illness Therapy Chemotherapy Convenience and Satisfaction Questionnaire (FACIT-CCSQ), respectively. Patients completed questionnaires at baseline, at cycle 4 (C4) and cycle 8 (C8) (FOLFOX4) or at cycle 3 (C3) and cycle 6 (C6) visits (XELOX) and at their final visit. Results: In the QLQ-C30, at the final visit, XELOX patients had better functional scores than FOLFOX4 patients (physical: 85.7 vs.60.4, p=0.03; role: 83.3 vs. 57.5, p=0.04) as well as better symptom scores (constipation: 9.5 vs. 40.4, p=0.01). In CIPN, at the C6/C8 visit, XELOX patients had lower motor scale scores than FOLFOX4 patients (3.8 vs. 21.6, p=0.02). Moreover, at the C6/C8 visit, XELOX was more convenient than FOLFOX4 in FACIT-CCSQ (79.7 vs. 55.5, p=0.04). Male patients were especially likely to consider XELOX to be more convenient (90.0 vs. 55.0, p=0.01) and satisfactory (55.4 vs. 26.2, p=0.03) and fewer concern (91.0 vs. 65.0, p=0.03) than FOLFOX4. XELOX patients spent fewer days on hospital visits at C3/C4, C6/C8 and final visit (2.8 vs. 4.2, p=0.01; 2.7 vs. 4.1, p=0.01; 3.0 vs. 4.5, p=0.01). Conclusion: XELOX may be a better adjuvant chemotherapy choice for patients with colon cancer than FOLFOX4 in terms of QoL, convenience, and satisfaction.

• Journal of the Korean Ceramic Society
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• v.40 no.1
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• pp.18-23
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• 2003

Composite powder of$B_4C-A1_2O_3$was prepared from a mixed powder of$B_2O_3/A1/C$by SHS under argon pressure instead of using a chemical furnace. A mixture of$B_2O_3,$Al and C powder (equivalent amounts to the reaction,$2B_2O_3+4A1+C=B_4C+2A1_2O_3)$was ball milled for 2 h. The mixed powder was placed in a SHS reactor and filled with 10 atm of argon gas and ignited. The inner and outer products were the same by XRD analysis. It was consisted of a composite powder of$B_4C-A1_2O_3$without $AlB_{12}/C_2$which was always produced using a chemical furnace. The composite powder was about$60~100{mu}m$size which was composed of crystalline particles of about 0.3~l${mu}m$size. But when 15 atm of argon was employed, partial sintering took place to give rise hard composite powder of$15~25{mu}m$$B_4C$with $0.1~0.2{mu}m$$A1_2O_3.$

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.223-228
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• 1985

Photosynthetic inhibition to temperature were conducted with ginseng(4 year old) and tobacco(var. Bulgaria). The plants were kept under various temperature conditions from 1$0^{\circ}C$ to 4$0^{\circ}C$ and 440$\mu$E/$m^2$/sec for 3 and 6hrs, and net $CO_2$ uptake were measured after 2hrs at $25^{\circ}C$. Photosynthetic optimal leaf temperature of ginseng was 21$^{\circ}C$ and tobacco was $25^{\circ}C$. Stomatal resistance and mesophyll resistance increased at high temperature. Especially, stomatal resistance seemed to have a significant role in determining the temperature responses of photosynthesis. In tobacco photosynthetic capacity was not changed by temperature treatment for 3hrs. However, 6hrs exposure reduced 8% of net photosynthesis at 4$0^{\circ}C$ and 12% at 1$0^{\circ}C$. Ginseng plants exposed for 6hrs at 4$0^{\circ}C$ lost photosynthetic capacity by 83%. Temperature responses of ginseng were very sensitive at above-optimum temperature resulting greater thermal inhibition other than photoinhibition.

• Journal of the Korean Ceramic Society
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• v.36 no.10
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• pp.1007-1015
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• 1999

The Si3N4 composites reinforced with carbon-coated SiC whiskers were fabricated by hot-pressing at 180$0^{\circ}C$ for 2 hours to examine the effects of carbon-coated whiskers on the mechanical properties of SiC whisker reinforced Si3N4 composites. The flexural strength of the Si3N4 composites and Si3N4 monolith respectively. The weak interfacial bond between carbon-coated SiC whiskers and Si3N4 matrix which enhances the crack deflection and whisker pull-out could contribute to the improvement of mechanical properties of the composites.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.4
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• pp.196-206
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• 2012

Studies of seasonal changes in $C_3/C_4$ mixed communities are rare, particularly in Asian summer monsoon climate zones. In our present study, seasonal changes in the profile and coverage of $C_3$ and $C_4$ plants were investigated in 2009 in Haenam, Yeongdong and Cheorwon regions of South Korea (all at different latitudes). The aim was to estimate the impacts of temperature and sunshine duration on species composition and transition timing of the $C_3$ and $C_4$ plants. From our results, the number of $C_3$ plants was found to increase from early spring to mid-May, and then decrease again until September in the Haenam and Yeongdong regions, but continuously increase from early spring to September in the Cheorwon region under relatively low summer temperatures. On the other hand, the number of $C_4$ plants increased from June or July to September in all three regions. These seasonal changes in species number and ratio have a direct impact upon species diversity which is highest when there are no dominant species. The relative coverage and relative summed dominance ratio (SDR') of the $C_3$ plants decreased from spring to autumn, but increased for the $C_4$ plants during this time in an exponential fashion with increasing accumulated temperature and sunshine duration. The transition timing from $C_3$ to $C_4$ plants occurred when the sum of sunshine duration for the days with daily mean temperature above $5^{\circ}C$ was 1017 hrs for the SDR'.