• YAKHAK HOEJI
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• v.50 no.4
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• pp.228-233
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• 2006

Matrix metalloproteinase-2 is known to be involved in pathological processes such as tumor invasion or rheumatoid arthritis. A soil microorganism producing siderophore under low iron stress $(up\;to\;5\;{\mu}m\;of\;iron)$ was identified as Burkholderia sp. Hydroxamate type siderophore produced by Burkholderia sp. CAS-5 was partially purified. MMP inhibitory activity of siderophore was confirmed by gelatin zymography. The $Zn^{2+}-chelating$ activity of siderophore correlated with the inhibition of MMP-2 activity.

• Korean Journal of Environmental Biology
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• v.28 no.2
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• pp.95-100
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• 2010

Burkholderia cepacia PBSA-7, Bacillus licheniformis PBSA-8 and Burkholderia sp. PBSA-9 previously collected from Korea soil (Joo and Kim, 2009) were analyzed for the presence of genes encoding proteins operative in the degradation of poly(butylene succinate-co-butylene adipate; PBSA). Polymerase chain reaction analyses revealed a 1.5 kb fragment of the lipase gene (lip A) in B. cepacia PBSA-7 and Burkholderia sp. PBSA-9, while B. licheniformis PBSA-8 harbored the same gene fragment at 600 bp. The three strains possessed "Gly-X1-Ser-X2-Gly" and "Ala-X1-Ser-X2-Gly" lipase sequence regions. Burkholderia sp. PBSA-7 lip A displayed 36~40% homology with the family 1-1 lipases and 82~92% homology with the family 1-5. Burkholderia sp. PBSA-8 lip A was 64~65% homologous with the subfamily 1-4 lipases, but displayed no homology with the subfamily 1-5 lipases. Burkholderia sp. PBSA-9 lip A displayed 35~37% homology with the family I1 lipases and 83~94% homology with the family I2 lipases, similar to Burkholderia sp. PBSA-7.

• Weed & Turfgrass Science
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• v.6 no.3
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• pp.249-261
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• 2017

To prevent large patch disease, caused by Rhizoctonia solani AG-2-2, in zoysiagrass a fungicide, Tebuconazole and three microbial agents Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 were applied in commercial turfgrass cultivation field in Sanchung, Gyeongnam, Korea. All treatments showed 50% reduced the pathogen population in thatch layer throughout the yearly cultivation period. Not only reduced the pathogen population, Tebuconazole, Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 treatment also enhanced turfgrass growth, chlorophyll and proline content. Malondialdehyde contents in each treatment was reduced from 6.2~28.9% when compared with the control. Taken together, reduction of pathogen population in soil lowered the disease incidence or severity, and allowed the turfgrass developed as normal condition. The results suggested that the selected microbial agents may use as biological control and growth promotion agents for the Zoysia turfgrass.

• KSBB Journal
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• v.15 no.6
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• pp.589-593
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• 2000

Several bacterial strains growing on benzene minimal medium were isolated from soil by enrichment culture, Burkholderia sp. SKK381 was identified and selected. In order to determine the ability of Burkholderia sp. SKK381 to degrade benzene. Changes in substrate concentration, cell growth, and pH were monitored from start-up in bath culture. At 30$^{\circ}C$, 1000 ppm of benzene was degraded 100% within 28hours. Cell growth conditions were best at an initial pH of 7.0 and a benzene concentration of 1000 ppm at 30$^{\circ}C$.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.3
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• pp.251-258
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• 2016

A rhizobacterium LPN-2, which showed strong antifungal activity and auxin producing ability, was isolated from a farmland in North Gyeongsang Province, South Korea. Based on analysis of the 16S rDNA sequence, strain LPN-2 was identified as a novel strain of Burkholderia and was designated as Burkholderia sp. LPN-2. In vitro experiments showed that the isolated stain LPN-2 significantly produced auxin within 48 hr incubation. In order to check for PGPR function we performed in vivo growth promoting test in different crops, including mung bean, pea and cabbage. Application of Burkholderia sp. LPN-2 showed dramatic growth promoting effect on all the tested plants. We also confirmed siderophore and cellulase productions by Burkholderia sp. LPN-2 using CAS blue agar and CMC plate test. Further treatment with LPN-2 and the crude culture broth was effective in suppressing anthracnose in vitro test and also reduced incidence and severity of anthracnose in apple and pepper. Taken together, we conclude that Burkholderia sp. LPN-2 might be used as organic fertilizer for effective crop production in organic farming.

• Journal of Bio-Environment Control
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• v.22 no.2
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• pp.100-107
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• 2013

Unlike general microwave, QRD (Quadratic Residue Diffusor) Microwave used in this study is known as a new technology that enhances the sterilization effect with low power because it is possible to induce the average sterilization by changing wavelength phase difference. Therefore, basic research was conducted on the function that could sterilize culture media for plant factory by using environmentally friendly and low energy consuming QRD Microwave. The results are as follows: It was confirmed that there was no external deformation in the polyurethane foam and rock wool medium when changing the microwave level between 2 and 8 kW in different water content of culture media. However, PDA solid media at 2 kW were not dissolved in 60 and 180 seconds. All of the media were dissolved in other processing. There was little difference in the microwave irradiation level and surface temperature of the strain according to the processing time between Bacillus sp. and Burkholderia sp. In the sterility test according to the microwave irradiation level and processing time, it was confirmed that both Bacillus sp. and Burkholderia sp. grew in the microwave level 2 kW regardless of time. In the microwave level 6 kW, all experimental groups except the processing of Burkholderia sp. for 60 seconds were sterilized, and all of Bacillus sp. was killed in the all experimental groups. In the microwave level 8 kW, it was confirmed that both Bacillus sp. and Burkholderia sp. were sterilized regardless of time. The temperature in microwave-processed media after contaminating strains to each medium was maintained at more than 100 in polyurethane foam and rock wool medium after 60 seconds. In general, it was shown that it was possible to sterilize after 60 seconds. Therefore, it is considered that Bacillus sp. and Burkholderia sp. which are the biggest problems in the plant factory can be adequately sterilized by QRD Microwave used in this study.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.116-123
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• 2000

The purpose of this work was to investigate the induction of stress shock proteins (SSPs) in Burkholderia sp. YK-2 in response to 2,4-dichlorophenoxyacetic acid (2,4-D), and Pseudomonas sp. DJ-12 to benzoate, 4-chlorobenzoate (4-CBA), 4-hydroxybenzoate, and biphenyl. The SSPs, which contribute to the resistance of the cytotoxic effect of the toxic aromatic compounds including 2,4-D and 4-CBA, were induced at different concentrations of the compounds in exponentially growing cultures of Burkholderia sp. YK-2 or Pseudomonas sp. DJ-12. This response involved the induction of a 43 kDa DnaK and 41 kDa GroEL proteins in Burkholderia sp. YK-2, characterized by SDS-PAGE and Western blot using the anti-DnaK and anti-GroEL monoclonal antibodies. In Pseudomonas sp. DJ-12, 70 kDa DnaK and 60 kDa GroEL proteins was induced as SSPs, respectively. The total SSPs were analyzed by 2-D PAGE. Survival of Burkholderia sp. YK-2 or Pseudomonas sp. DJ-12 with time in the presence of different concentrations of the compounds was monitored, and viable counts paralleled the induction of the SSPs in these strains. Cells treated with the increased concentrations of toxic compounds showed some destructive openings on the cell envelopes.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.643-650
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• 2000

A new aniline-utilizing microorganism, strain HY1 obtained from an orchard soil, was characterized by using the BIOLOG system, an analysis of the total cellular fatty acids, and a 16S rDNA sequence. Strain HY1 was identified as a Burkholderia species, and was designated Burkholderia sp. HY1. GC and HPLC analyses revealed that Burkholderia sp. HY1 was able to degrade aniline to produce catechol, which was subsequently converted to cis,cis-muconic acid through an ortho-ring fission pathway under aerobic conditions. Strain HY1 exhibited a drastic reduction in the rate of aniline degradation when glucose was added to the aniline media. However, the addition of peptone or nitrate to the aniline media dramatically accelerated the rate of aniline degradation. A fatty acid analysis showed that strain HY1 was able to produce lipids 16:0 2OH, and 11 methyl 18:1 ${\omega}7c$ approximately 3.7-, 2.2-, and 6-fold more, respectively, when grown on aniline media than when grown on TSA. An analysison the alignment of a 1,435 bp fragment. A phylogenetic analysis of the 16S rDNA sequence based on a 1,420 bp multi-alignment sowed of the 16s rDNA sequence revealed that strain HY1 was very closely related to Burkholderia graminis with 95% similarity based that strain HY1 was placed among three major clonal types of $\beta$-Proteobacteria, including Burkholderia graminis, Burkholderia phenazinium, and Burkholderia glathei. The sequence GAT(C or G)${\b{G}}$, which is highly conserved in several locations in the 16S rDNA gene among the major clonal type strains of $\beta$-Proteobacteria, was frequently replaced with GAT(C or G)${\b{A}}$ in the 16S rDNA sequence from strain HY1.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.131-139
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• 2007

From the course of screening of useful enzyme producing microorganism from insect guts, we isolated 9 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram negative bacteria grouped on Serratia sp., Pseudomonas sp., and Burkholderia sp.. Among them, an excellent lipase producing strain, Burkholderia sp. HY-10 identified by 16S rDNA analysis and biochemical methods, was further studied its lipase producing characteristics. It was isolated from a longcorm beetle, Prionus insularis and showed cell density dependent lipase producing activity in the culture media that contained olive oil as a carbon source. Maximum lipase production was achieved in the M9 media containing 0.5% yeast extract and 0.5% olive oil when cultured at $30^{\circ}C$ for 36-42 hrs.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.1
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• pp.112-117
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• 2011

A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg $L^{-1}$ after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg $ha^{-1}$) soil inoculation with Burkholderia sp. (Twenty milliliters/plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.