• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Journal of Korean Society of Forest Science
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• v.97 no.2
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• pp.181-186
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• 2008

Even small changes in phosphorus concentrations in stream water could cause eutrophication because of very low level of phosphorus concentrations in natural waters. I investigated the impact of strip cut and clear cut on phosphorus concentrations in stream water at the Hubbard Brook Experimental Forest and investigated stream water phosphorus concentrations as a function of flow rate and season (as well as cutting history). Mean phosphate concentrations in the control (undisturbed forest) increased $1.9{\mu}g\;L^{-1}\;to\;2.6{\mu}g\;L^{-1}$, while strip cut treatment increased phosphate concentrations in stream water $2.2{\mu}g\;L^{-1}\;to\;3.7{\mu}g\;L^{-1}$ during the same period. There was no significant effect of clear cut treatment on phosphate concentrations in stream water. No relationships were found between discharge rate and phosphate concentrations, but the magnitude of fluctuation were increased during two decades in undisturbed forest: $1-5{\mu}g\;L^{-1}$ from 1963 to 1975 and $1-12{\mu}g\;L^{-1}$ from 1983 to 1995. Based on this study, forest harvests with buffer zone will not make a problem by imported phosphate to cause eutrophication in natural water.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2005.11a
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• pp.161-162
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• 2005

2G HTS coated conductor wire consists of textured substrate, buffer layer, superconduct layer, Ag cap layer, stabilizer. For practical application filed, coated conductor have mechanical and electrical stability and environment protection properties. This property Cu and stainless steel strip is laminated to Ag cap layer as stabilizer materials. Lamination process join stabilizer material strip and Ag cap layer with soldering method. we have laminated HTS with continuous dipping soldering process different stabilizer Cu and stainless steel strip and changed lamination process condition. The effect of lamination stabilizer and process condition has been investigated mechanical and electrical properties.

• Journal of Environmental Impact Assessment
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• v.24 no.5
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• pp.516-531
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• 2015

In this study, a software module to predict the effectiveness of vegetation buffer strip (VBS) has been developed for using with Chemicals, Agricultural Management and Erosion Losses (CAMEL), a distributed watershed model. Most basic functions for the VBS module are same as CAMEL except functions newly developed to implement sedimentation enhancement by vegetation and level spreaders. For verification of the VBS module, sensitivity analyses for length, roughness, soil and vegetation type of VBS were carried out using a test grid cell. The surface discharge of sediment are highly sensitive to the roughness coefficient of VBS. The removal efficiencies of VBS for the surface discharges of sediment and TP are generally high regardless of environment changes. The surface discharges of TOC and TN are highly sensitive to the length and soil of VBS. The removal efficiencies of VBS for the surface discharges of TOC and TN are generally lower than those of sediment and TP. The newly developed VBS module reasonably simulates the removal efficiencies of surface discharges that vary according to the environment changes. It is expected that this VBS module can be used for evaluating the effectiveness of VBS-based best management practices to be applied to reduce pollution discharges from various non-point sources.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.29A no.8
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• pp.41-48
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• 1992

The characteristics of traveling-wave electrodes for high-frequency electroptic integrated devices are described from the view point of improvement of phase-matching based on the conformal mapping method. Specific calculations of the characteristic impedance, effective microwave index, and eletrode loss for asymmetric coplanar strip(ACPS) and coplanar waveguide(CPW) electrode structures are presented as a function of the geometric electrode parameters including the electrode thickness and buffer layer thickness. 5-10(x10S0-6Tm) thick Au-ACPS electrodes were successfully fabricated by electroplating and ECR etcher. The improvement of modulation bandwidth can be theoretically observed from the combination of electrode and buffer layer thickness.

• Environmental Engineering Research
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• v.25 no.1
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• pp.123-128
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• 2020

Non-point source pollutant load reductions were calculated using the Hydrologic Simulation Program-Fortran (HSPF) model under the assumption that landuse pattern was changed according to land purchases. Upon the simulation of non-point pollutant and areas with high land purchase ratios to select a buffer zone, the Namgang dam Reach 11, Imha dam Reach 10, and the Reach 136 watershed of the main river were found to rank high for the construction of buffer zones. Assuming that the forms of the purchased lands were changed to wetlands, biological oxygen demand (BOD) loads were changed through the HSPF model. No changes of BOD were present in the Namgang dam and the Imha dam watersheds. BOD loads in Reach 136 according to landuse change were analyzed through a flow duration analysis based on the total maximum daily loads of the United States. The flow duration analyses undertaken to examine changes in BOD of main river Reach 136 watershed indicated a shift of 0.64 kg/d from 3.16 to 2.52 during high flow. The change of BOD under the conditions of moist, mid-range and dry were 11.9%, 9% and 4.5%. At the low flow condition, the variation range in the BOD load was from 0.58 kg/d to 0.41 kg/d.

• Journal of the Korean Chemical Society
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• v.47 no.4
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• pp.339-344
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• 2003

Paraquat is an effective and widely used herbicide, but it is also very toxic to humans. It is well-known that urine paraquat concentration is one of the most important prognostic indicator for paraquat-poisoning. Quantitative analysis of paraquat, however, are not generally used in clinical laboratories. In this work, a new test strip to detect paraquat concentration using sodium dithionite in urine was developed. Using these second-derivative method, the test strip prepared in $0.5{\%}$ borate buffer (pH 8.0), 0.25 M $Na_2S_2O_4,\0.1~0.8{\%}$ PVP, and $1{\%}$ decanol showed not only better color reaction but also an excellent application possibility to be used in automatic analyzer.

• Journal of Korean Society of Forest Science
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• v.83 no.4
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• pp.545-555
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• 1994

The objective of the study was to provide the useful scientific data on the early rehabilitation of the legging road after timber harvesting in the forest area. This study was carried out at logging roads which were constructed during 1989 and 1994 in Mt. Baekwoon. The field survey was conducted in July, 1991. Judging from the analysis of soil bulk density, time required for recovery as the undisturbed forest soil condition was more than 10 years in the road which was left, and the regression equation is as follows, $$Y_1=1.4195-0.0744{\cdot}X(R^2=0.91)$$ $$Y_2=1.4673-0.0688{\cdot}X(R^2=0.73)$$ (X : elapsed year after road construction. $Y_1$, $Y_2$ : soil bulk density($g/cm^3$) at 0~7.5cm, and 7.5~15.0cm, respectively) Especially soil bulk density with buffer strip-woods was $0.890-0.903g/cm^3$, so it was 20% lower than that of logging road surface without buffer strip-woods. Among the 7 factors, location, sand content, and soil hardness had statistically significant effect on the soil bulk density in logging road surface. The pioneer species on logging road surface were Rhus cratargifolius, Prunus chinensis, and Lespedeza cyrtobotrya, etc. in woody species, and Pteridium aquilinum, Arundinella hirta, and Lysimachia clethroides, etc. in herb species. So, in process of year, average plant coverage were 70% on cutting and banking slope and 20% on logging road surface which elapsed 6 years after logging road construction. Through this research, buffer strip-woods must be remained for environmental conservation of forest conditions, and from the time to be closed the road, planting, seeding, and grazing works could be effective to the soil condition and vegetation recovery.

• KSBB Journal
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• v.18 no.1
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• pp.35-38
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• 2003

Analysis of proteome in amniotic fluid was performed by 2-D PAGE (polyacrylamide gel electrophoresis). Proteins in amniotic fluid were separated by centrifugation and solubilized in buffer solution for IEF, using an IPG strip of pH 4-7L. Both a homogeneous slab gel of 12.5% and a gradient gel of 8-18%, were used. After 2-D PAGE, spots were stained with silver nitrate and picked up for in-gel digestion. Digested peptides were analyzed by MALDI-TOF and proteins were further identifical. More protein spots were detected in the gradient gels and a protein not previously reported was identified.

• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.1
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• pp.67-74
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• 2005

The aim of this study was to analyze the relationship between salivary flow, salivary buffer capacity, level of mutans streptococci and dental caries experience in Korean dental college students. A total of 81 students of the school of dentistry, Chonbuk national university, mean age of 26.1 years, were subjected to oral examination to establish the DMFT and DMFS according to WHO guidelines. Saliva samples from the students were collected for quantitating stimulated and unstimulated salivary flow rate. Buffering capacity was estimated using a commercial colorimetric strip test, and number of mutans streptococci was determined from stimulated saliva using a strip mutans test. The means of DMFT and DMFS were 6.57 and 12.65, respectively. The stimulated salivary flow rate was correlated with DMFT(${\acute{o}}\;=\;-0.219$) and high levels of salivary mutans streptococci were significantly correlated with higher DMFT and DMFS scores(P<0.05). Level of mutans streptococci was significantly correlated with dental caries experience, in both DMFT and DMFS score, and buffering capacity was inversely correlated to DMFT score. However, unstimulated salivary flow rate was not correlated with caries experience.