• 화훼연구
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• 제18권4호
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• pp.278-283
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• 2010

본 시험은 국화에서의 기형화 발생과 DNA 메틸레이션에 관여하는 S-adenosyl-L-homocysteine hydrolase (SAHH) 유전자 발현과의 관련성을 검토하기 위해 수행하였다. 스프레이 국화 'Lerbin'은 단일후 14일에서 27일 사이에 고온과 장일 조건에서 기형화가 발생되어, $35/20^{\circ}C$의 고온과 14시간의 장일 처리 할 경우 $25/20^{\circ}C$(12 h/12 h)에 비해 설상화가 2배 이상 증가하는 양상을 보였다. 스프레이 국화 'Lerbin'에서 분리한 전장 cDNA (DgSAHH)는 크기가 1455 bp로 다른 작물과 90%이상의 높은 상동성을 나타내었다. 국화의 DgSAHH 유전자 발현은 기형화 발생을 유도하는 고온과 장일 조건에서 크게 감소하였다. 이러한 결과를 통해 국화의 화아 발달에 있어서 DgSAHH 유전자 발현은 온도와 일장의 영향을 받으며, 억제된 이 유전자의 발현이 기형화 발생에 관여할 수 있을 것으로 판단된다.

• 한국작물학회지
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• 제51권spc1호
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• pp.242-246
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• 2006

국내 재배 유채의 육종을 위해서는 소포자배양을 통한 배발생법의 확립이 절실하나 대부분의 재배 품종이 배발생율이 낮고 품종별로 화뢰의 크기에 따른 소포자의 발달 상태가 다르기 때문에 이에 대한 조사가 반드시 필요하다. 따라서 본 연구에서는 국내 육성 유채 품종의 육종을 위한 기초 자료로서 품종 간 화뢰의 크기에 따른 생체 내 화분(핵)발육 상태를 비교, 관찰하였다. 1. 소포자 배양에 있어서 배 발생이 가능한 소포자 분리 시기는 1핵기말기 또는 2핵기초기가 효과적이며, 소포자 배양에 최적인 화뢰의 크기는 품종에 따라 차이가 있지만 3.5-4.0 mm이다. 2. 예외적으로 '탐라유채'는 3.5-4.0 mm의 크기에서 다수의 1핵기말기의 소포자와 2핵기초기의 소포자가 관찰되었으며, 화뢰크기가 더 큰 4.0-4.5 mm 크기에서도 2핵기초기의 핵이 관찰되어 화분발육상태가 늦게 진행되는 것을 알 수 있었다. 3. '목포64호'의 경우 3.0-3.5 m크기에서 1핵기초기와 1핵기중기 및 1핵기 말기의 소포자들이 관찰되었으나 다른 품종과 달리 2핵기 화분이 전혀 관찰이 되지 않았다. 'MS-B Line'의 경우는 2.5-3.0 mm 크기에서도 4분자포자가 관찰되어 초기 발육상태가 조금 늦게 일어나는 것을 볼 수 있었다.

• 한국환경복원기술학회지
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• 제6권1호
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• pp.41-50
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• 2003

The feasibility as floricultural crops and water garden plant materials of Korean native water plants was investigated. Propagation type, growing and flowering time were observed for development as water garden, interior aquarium plant and water purification materials. Flowering time of the water plant was 7 species in May, 28 species in June, 49 species in July, 55 species in August, 47 species in September, and 17 species in October. Beautiful flowering water plants were Nymphaeaceae, Nymphoides peltata, Nymphoides indica, Monochoria korsakowii, Iris pseudacorus, Iris laevigata, and etc. Ornamental leafy water plants were Ceratopteris thalictroides, Ludwigia ovalis, Myriophyllum verticillatim, Limnophila sessiliflora, Blyxa aubertii, Blyxa echinosperma, Vallisneria asiatica, Hydrilla verticillata and Eleocharis acicularis etc. Isoetes japonica, Isoetes coreana and Isoetes sinensis were propagated by spore. Blyxa aubertii, Blyxa echinosperma, Myriophyllum verticillatim, Nuphar japonicum, Nelumbo nucifera, Ottelia alismoides, Sagittaria aginashi, Trapa japonica, and Trapa natans were propagated by seed. Persicaria amphibia, Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Oenanthe javanica, Potamogeton crispus, Hydrilla verticillata and Acorus calamus were propagated by division. And Vallisneria asiatica, Hydrilla verticillata and Phragmites japonica were propagated by runner. Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Limnophila sessilifera were propagated by adventitious bud. Ceratopteris thalictroides was propagated by leaf cutting. The 35 genera, 68 species of water plants were available for horticultural use. The 45 species such as Iris laevigata, Eleocharis acicularis, Menyanthes trifoliata, Nymphaea minima, Nuphar pumilum, Nymphoides coreana, Nymphoides peltata, Nymphoides indica, Nymphaea tetragona (water lily), and Typha latifolia could be use for water garden plant. The 21 species such as Limnophila sessilifera, Vallisneria asiatica, Ceratophyllum demersum and Hydrilla verticillata available for indoor aquarium. The 19 species such as Ottelia alismoides, Oenanthe javanica, Limnophila sessilifera and Blyxa echinosperma could be culture in container. The 27 species such as Trapa japonica, Trapa incisa, Phramites commuris (reed), Phragmites japonica, and Zizania latifolia were usable for water purification plant materials.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제25권4호
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• pp.311-323
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• 1999

The osteonectin is a sort of glycoprotein which is secreted in human tissues. The osteonectin is generally detected in number of normal or neoplastic human tissues in vivo, but hasn't been studied the role of osteonectin in developing human teeth and odontogenic tumors. We evaluated degree of the expression of osteonectin immunohistochemically in 20 cases of developing tooth germ which growth from fetus 5 to 38 weeks, and total 51 odontogenic tumors whitch has taken from routine biopsy, such as 10 ameloblastomas, 5 cases of adenomatoid odontogenic tumors and odontomas and odontogenic fibromas, 4 cases of cementomas and calcifying epithelial odontogenic cyst and odontogenic keratocyst and dentigerous cysts and periapical cysts, and 3 cases of ameloblastic fibromas and myxomas. The results were as follows: 1. The osteonectin on the bud stage of tooth germ was strongly expressed in the epithelial dental lamina and in the outer dental epithelium on the early bell stage, and also strongly expressed in the inner dental epithelium on the late bell stage of tooth germs. 2. In ameloblastoma, the osteonectin was strongly expressed in the epithelial tumor component and especially in the acanthomatous types. 3. In both of calcifying epithelial odontogenic tumor and adenomatoid odontogenic tumors, the osteonectin was moderately expressed on the duct like spindle cells and epithelial tumor cells around calcification areas. 4. In odontogenic tumors originated from epithelial-mesenchymal tissues, the osteonectin was moderately expressed on the epithelial tumor components and in odontogenic cysts, it was expressed in ghost cells and calcification areas only. These were summaried the osteonectin may be strongly related to the developing tooth germ and odontogenic tumors and could be regulated hard tissue of human tooth in morphogenesis and involved with calcification mechanism in development odontogenic tumors.

• 한국작물학회지
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• 제51권spc1호
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• pp.297-303
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• 2006

Cuticular waxes on tea (Camellia sinensis L.) loaves consisted mainly of alkanes, fatty acids, primary alcohols, triterpenes, and a group of unknown compounds, dominated by primary alcohols and triterpenes. Tea tree accessions used in this study were M-1, M-2, Sakimidori, and Yabukita. For all accessions, the alkane, fatty acid, and primary alcohol constituents consisted of a homologues series, and the major constituents of primary alcohol class were the C28 and C30 homologues. Triterpenes consisted of friedelin, $\beta-amyrin$, and three unidentified ones and friedelin was the most abundant. Leaf area and the total amounts of cuticular waxes per leaf increased with lower leaf position from the apical bud in Yabukita variety. With different leaf position, total wax amount per unit leaf area on the youngest leaves of P1 (the uppermost leaf position) showed the largest amount $(12.80{\mu}g/cm^2)$, and on mature loaves of P2 to P6 ranged from 7.08 to $7.77{\mu}g/cm^2$, and then on the oldest loaves of P7 (the lowest leaf position) remained at an increased level $(17.53{\mu}g/cm^2)$. During leaf development (lower leaf position), the amount of primary alcohols decreased from P1 to P6 and increased at P7, whereas that of triterpenes increased from P1 to P7. The percentage of each wax class in the total wax amount occurred a decrease in primary alcohol and an increase in triterpene, with leaf age.

• 한국도서관정보학회지
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• 제31권2호
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• pp.253-278
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• 2000

이 연구는 DDC를 사용하는 한국도서관에서 종교류를 재전개하기 위한 시안을 제시하기 위한 것이다. 이를 위하여 우선 DDC 의 종교류에 마련된 임의규정의 발전 과정과 문제점을 살펴보고, DDC 종교류의 임의규정은 단일종교가 그 나라에서 대표성을 갖는 경우에 대비한 것으로, 한국과 같이 다수의 종교가 공존하는 나라의 경우에는 이를 적용하기가 어렵다는 점을 밝혔다. 이 연구에서 제시한 재전개안의 특징은 DDC 의 기본적인 체계와 배열순서를 유지하면서도, 한국의 실정에 맞도록 불교와 동양의 종교를 강조할 수 있도록 한 점이다. 이를 위해 DDC 원안의 220-280의 7개 강에 전개된 기독교를 220 하나의 d로 축소하였고, 230부터 270에는 불교를 비롯한 그 밖의 종교를 배정하여, 여러종교간의 균형을 유지하였고, 특히 동양3국의 주요종교를 상위에 배치시켰다. 아울러 이 안에서는 불교와 동양종교, 한국종교를 강조하고자 할 경우에 대비한 별법(別法)도 제시하였다.

• 농촌지도와개발
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• 제7권1호
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• pp.167-173
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• 2000

'거봉' 포도재배시 발생하는 결실불량 현상을 방지하기 위한 적절한 전정방법과 결과모지 선별방법을 구명하기 위하여 안성의 농가 포장과 원예연구소의 포장에서 수행한 연구결과는 다음과 같다. 약전정은 강전정에 비해 발아율, 신초수, 과방중, 착립률을 증가시킨 반면, 신초장, 신초당 엽수와 마디수, 단위 엽면적, 그리고 단위 엽생체중은 감소시켰다. 과실의 당도, 산함량 및 안토시아닌 함량은 약전정구와 강전정구간에 차이가 없었다. 평균신초장은 결과모지의 굵기가 굵을수록 뚜렷이 길어지는 경향을 나타내었으며, 엽면적과 엽록소함량은 결과모지의 굵기가 굵을수록 넓어지거나 높아지는 것으로 나타났다. 총탄수화물 및 N, P, K, Ca 및 Mg 등 무기성분 할량은 결과모지 굵기에 따라 유의한 차이를 보이지 않았으나 총탄수화물과 Ca함량은 결과모지 굵기가 가늘수록 높아지는 경향을 보였다. 과방당유핵과립수는 결과모지가 가늘수록 적어지고 착립률은 높아지는 경향을 나타냈다. 과방중, 과립중, 과방당 과립수, 당도, 안토시아닌 함량은 결과모지 굵기가 1cm 전후인 구에서 높게 나타났다.

• 동의생리병리학회지
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• 제31권3호
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• pp.188-193
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• 2017

This study investigated the antioxidant activities of distilled water, ethanol and methanol extracts of Plunus mume flower buds (PFB). The various solvent extracts of PFB were evaluated for their total polyphenol, flavonoid, reducing power and free radical scavenging activities by FRAP and DPPH analysis. The ethanol extract of PFB contained significantly higher amounts of total polyphenols (145 mg GAE/g) and flavonoids (25.43 mg QE/g) than methanol (132 and 25.42) and distilled water (113.6 and 18.04). Among solvent extracts of PFB, the ethanol extract showed the highest antioxidant activities. The 100% ethanol extract of PFB contained significantly higher amounts of total polyphenols and flavonoids than 70% and 50% ethanol extracts. Moreover, the 100% ethanol extract of PFB showed high efficacy in DPPH radical scavenging activity and in collagenase inhibition activity. This results suggest that 100% ethanol extract of PFB has the most effective antioxidant capacity compared to the methanol and water extracts tested in the present study. Thus, it can be applied for the effective extraction of functional material from PFB for usage of cosmeceutical and/or food industries.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.89-94
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• 2001

In vitro plant regeneration of inbred breeding line of hot pepper (Capsicum annuum L.) was established using leaf and petiole segments as explants. About 28 days old plants were excised and cultured on MS medium supplemented with TDZ and NAA or in combination with Zeatin. In all of the media compositions tested, combination of TDZ 0.5 mg/L, Zeatin 0.5 mg/L, and NAA 0.1 mg/L was found to be the best medium for shoot bud initiation. Young petiole was the most appropriate explant type for the plant regeneration as well as genetic transformation in hot pepper. In this study, HpMADS1 gene isolated from hot pepper was introduced using Agrobacterium-mediated transformation system. Based on the analysis of Southern blot and RT-PCR, HpMADS1 gene was integrated in the hot pepper genome. It has been known that floral organ development is controlled by a group of regulatory factors containing the MADS domain. Morphological characteristics in these transgenic plants, especially flowering habit, however, were not significantly altered, indicating this MADS gene, HpMADS1 may be non-functional in this case.

• 건축역사연구
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• 제9권1호
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• pp.27-46
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• 2000

This study aims at examining the background and development process of Masan city after Koryo Dynasty, making a reconstructive map of Original Masan using the first registration maps made in 1912 and analyzing the urban structure of the Original Masan area. The origin of Masan city went back to Koryo dynasty. Around 1040 Koryo government placed an official shipping facility in Masan to transport the taxed grain to the capital city. After that Masan became an important port covering the southern part of KyungSang-Do. And the urbanization of the Original Masan began to bud after a shipping facility was again established by the Chosun Dynasty in 1760 and strengthened in the 19th century after the social standing system was broken and many peasants moved to cities as daily workers. In 1899 Masan was forced to be opened to foreign powers and they placed an international settlement distanced from the area(Original Masan). After this many Japanese advanced to the international settlement and further to the area. The advancement of Japanese brought a critical change in the urban structure of the area. Land was owned by Japanese and many modern sysytems were introduced such as modern buildings, new roads, railroad and modern factories. According to the reconstructive map of the area, 80.5% of land lots are less than $200m^2$ showing the size of each land lot is comparatively small. And Japanese occupied 31.5% of the land in the area. Their land was located on the stratigic points near the port and the center of commerce. The ratio of the road area to the whole land was around 14%, not so low for urban areas in that time. The reconstructive map reveals the exact coastline of the year 1912 erased now due to filling the sea and extending the land, Present Masan city has been developed having the Original Masan as its nucleus. The area has been always the center of urban activities for Masan city. Making an exact reconstructive map for the area and analyzing the urban structure of the area in 1912 is a very important work to understand Masan city wholy.