• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1132-1139
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• 2016

Brucellosis is a zoonotic disease caused by Brucella, a genus of gram-negative bacteria. Cytokines have key roles in the activation of innate and acquired immunities. Despite several research attempts to reveal the immune responses, the mechanism of Brucella infection remains unclear. Therefore, immune responses were analyzed in mice immunized with nine recombinant proteins. Cytokine production profiles were analyzed in the RAW 264.7 cells and naive splenocytes after stimulation with three recombinant proteins, metal-dependent hydrolase (r0628), bacterioferritin (rBfr), and thiamine transporter substrate-binding protein (rTbpA). Immune responses were analyzed by ELISA and ELISpot assay after immunization with proteins in mice. The production levels of NO, TNF-α, and IL-6 were time-dependently increased after having been stimulated with proteins in the RAW 264.7 cells. In naive splenocytes, the production of IFN-γ and IL-2 was increased after stimulation with the proteins. It was concluded that two recombinant proteins, r0628 and rTbpA, showed strong immunogenicity that was induced with Th1-related cytokines IFN-γ, IL-2, and TNF-α more than Th2-related cytokines IL-6, IL-4, and IL-5 in vitro. Conversely, a humoral immune response was activated by increasing the number of antigen-secreting cells specifically. Furthermore, these could be candidate diagnosis antigens for better understanding of brucellosis.

• Journal of Veterinary Science
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• 제22권2호
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• pp.15.1-15.13
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• 2021

Background: Attenuated Salmonella strain can be used as a vector to transport immunogens to the host antigen-binding sites. Objectives: The study aimed to determine the protective efficacy of attenuated Salmonella strain expressing highly conserved Brucella immunogens in goats. Methods: Goats were vaccinated with Salmonella vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10⁹ CFU/mL and challenge of all groups was done at 6 weeks after vaccination. Results: Among these vaccines inoculated at 5 × 10⁹ CFU/mL in 1 mL, Omp19 or SOD showed significantly higher serum immunoglobulin G titers at (2, 4, and 6) weeks post-vaccination, compared to the vector control. Interferon-γ production in response to individual antigens was significantly higher in SOD, Omp19, PrpA, and BLS individual groups, compared to that in the vector control (all p < 0.05). Brucella colonization rate at 8 weeks post-challenge showed that most vaccine-treated groups exhibited significantly increased protection by demonstrating reduced numbers of Brucella in tissues collected from vaccinated groups. Real-time polymerase chain reaction revealed that Brucella antigen expression levels were reduced in the spleen, kidney, and parotid lymph node of vaccinated goats, compared to the non-vaccinated goats. Besides, treatment with vaccine expressing individual antigens ameliorated brucellosis-related histopathological lesions. Conclusions: These results delineated that BLS, Omp19, PrpA, and SOD proteins achieved a definite level of protection, indicating that Salmonella Typhimurium successfully delivered Brucella antigens, and that individual vaccines could differentially elicit an antigen-specific immune response.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.89-92
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• 2011

The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.

• 한국환경보건학회지
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• 제15권2호
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• pp.117-121
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• 1989

An observation on the outbreak patterns of zoonoses was carried out on 261,862 cattle, 1,967 swine, 91,500 fowl samples in Chronnam Province. And it was classified by ppsitive.reaction from numbers of request examined and diagnosis to Animal Health Center of Chonnam Province from 1983 to 1988. The results are summarized as follows: Incidence rate of zoonoses in Chonnam area was observed in the order of alveolar sarcoma of fowl (75.0%), bovine facioliasis (47.6%), bovine mastitis (19.0%), bovine salmonellosis (12.6%), salmonellosis of swine (11.0%), salmonellosis of fowl (8.9%), bovine streptogenes (6.7%), tetanus of bovine (2.0%), streptogenes of swine (1.7%), pullorum disease (0.6%), bovine tubercullosis (0.02%), and bovine brucellosis (0.01%). And, especially bovine facioliasis was observed highest outbreaks (89.4%).

• 한국동물위생학회지
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• 제21권2호
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• pp.107-115
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• 1998

In order to establish a rapid, sensitive and specific diagnostic method for detection of antibody to Brucella abortus, a enzyme-linked immunosorbent assay(ELISA) was adapted. The diagnostic efficacy of the established ELISA was compared with that of the standard tube agglutination test for B abortus. 1. It was found that the optimal concentration of antigen for this ELISA was 5$\mu\textrm{g}$/ml, the optimal dilution of conjugate was 1 : 2000, and the optimal dilution of serum was 1 : 200, respectively. 2. Cut off value in this ELISA was 1,102 that was determined by mean absorbance(at 492nm) of tube agglutination test negative serum added with the triple value of the standared devation. 3. The relationship between the tube agglutination test and ELISA was showen high corresponding rate with sensitivity(96.3%) and specificity(98.1%). 4. The efficacy of the ELISA for detection of B abortus antibody was compared with tube agglutination test In brucellosis outbreak farm. The sensivity of ELSIA was higher than tube agglutination test.

• 한국동물위생학회지
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• 제32권4호
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• pp.347-351
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• 2009

Bruella spp. involves a considerable danger of public health and farm animal industry. In this study, we assessed the disinfection efficacy of alkaline disinfectant solution and three commercial farm disinfectants (quaternary ammonium compound, sodium dichloroisocyanurate, potassium monopersulphate/sodium dichloroisocyanurate) against Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of selected disinfectants following exposure to test bacteria for 30 minutes at $4^{\circ}C$. Disinfectants and test bacteria are diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. Three commercial disinfectant showed excellent antimicrobial activity (up to dilution of $\times200$ in OM treatment). Alkaline disinfectant solution demonstrated favorable bactericidal efficacy against B. abortus (at dilution of $\times20$ in OM treatment). Three commercial farm disinfectants possess excellent efficacy against B. ovis. Alkaline disinfectant solution has lower potency than commercial farm disinfectant but could help to limit the spread of brucellosis.

• 한국동물위생학회지
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• 제30권1호
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• pp.95-102
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• 2007

The purpose of this study was to investigate the seroprevalence of infection with the production-limiting diseases in dairy cattle in Jeonbuk-Iksan area. The blood samples were collected from 260 dairy cows in 52 herds, and examined. The antibody rates against N caninum, M paratuberculosis, and bovine leukemia virus were 34.6%, 13.5% and 89.6%, respectively. All samples for bovine brucellosis were negative.

• 대한수의학회지
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• 제28권2호
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• pp.339-343
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• 1988

Serological study and isolation of Brucellae from dairy cattle were carried out in Kyungpook area of Korea during the period from 1984 to 1985. Biotyping of the isolates was done. Of the 11168 cattle in 2479 herds examined, 27 cattle (0.24%) in the 17 herds (0.69%) were serologically positive for brucellosis. Brucella abortus was recovered from 20 (74.1%) of the 27 infected cattle, and all the strains of the isolates belonged to biotype 1. Of the tissues cultured for brucellae, the supramammary lymph nodes had the highest rate (55.6%) of recovery of brucella organisms.

• Asian-Australasian Journal of Animal Sciences
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• 제24권3호
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• pp.429-438
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• 2011

Mastitis is characterized by physical, chemical and bacteriological changes in the milk and pathological changes in the glandular tissue of the udder and affects the quality and quantity of milk. The bacterial contamination of milk from the affected cows render it unfit for human consumption and provides a mechanism of spread of diseases like tuberculosis, sore-throat, Q-fever, brucellosis, leptospirosis etc. and has zoonotic importance. Somatic cell count (SCC) is a useful predictor of intramammary infection (IMI) that includes leucocytes (75%) i.e. neutrophils, macrophages, lymphocytes, erythrocytes and epithelial cells (25%). Leucocytes increase in response to bacterial infection, tissue injury and stress. Somatic cells are protective for the animal body and fight infectious organisms. An elevated SCC in milk has a negative influence on the quality of raw milk. Subclinical mastitis is always related to low milk production, changes to milk consistency (density), reduced possibility of adequate milk processing, low protein and high risk for milk hygiene since it may even contain pathogenic organisms. This review collects and collates relevant publications on the subject.

• 한국동물위생학회지
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• 제29권1호
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• pp.47-53
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• 2006

For examination of antibiotic therapeutic efficacy in canine brucellosis, this examination was carried out two female bitches infected with Brucella canis in Gyeongbuk province, and used combicillin, baytril and doxycycline in susceptible antibiotics at B canis. During 18 month after the termination of antibiotic therapy, blood sample of the two bitches were examined for B canis antibody and antigen. The antibody of one bitch was disappeared at 5 month after antibiotic therapy and the other was continued at 18 month, but two bitches were not detected antigen by blood culture and PCR. Examination of blood chemical value (AST, ALT, urea, creatinine) of two bitches was increased in AST value during antibiotic therapy.