• 대한한방내과학회지
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• 제21권3호
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• pp.477-486
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• 2000

Objective : This is the experimental paper to investigate the effects of Gami-oryungsan(GO) on decreasing the activities of free radicals. Methods : We used three different group; In the normal group, we injected Gami-oryungsan extract intraperitoneally daily for 15days(90mg/kg), bromobenzene(310mg/kg) for 2days and injected normal saline in the control group. Results : We have observed the effects of Gami-oryungsan about the damage of rat's liver induced by bromobenzene. We can find the level of lipid peroxidation and type conversion ratio of xanthine oxidase decreased compared to the case of bromobenzene-treated group. The enzyme of activities of superoxide dismutase, catalase and glutathione peroxidase highly increased in Gami-oryungsan pre-acupunctured group compared to the group treated with only bromobenzene. The level of glutathione in Gami-oryungsan pre-acupunctured group was increased as highly as normal group. Also it was not seen special effects concerning aldehyde oxidase. Conclusions : Gami-oryungsan extract recovers the damage of liver due to bromobenzene intoxication by decreasing the lipid peroxidation.

• 한국약용작물학회지
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• 제15권1호
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• pp.21-25
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• 2007

해당화 뿌리는 우리나라 민간에서 당뇨병 치료제로 사용되는 약용식물이다. Bromobenzene으로 간독성을 유발한 흰쥐에 뿌리에서 분리한 화합물인 (+)-catechin을 경구투여하여 bromobenzene대사계에 미치는 효소활성을 간독성 물질인 bromobenzene 3,4-oxide 생성에 관여하는 효소인 aminopyrine N-demetylase와 aniline hydroxylase와 독성 epoxide 대사중간체를 무독화 시키는 epoxide hydrolase와 glutathione S-transferase에 활성을 관찰하였다. (+)-Catechin의 투여가 aminopyrine N-demetylase, aniline hydroxylase 및 glutathione S-transferase에 활성에는 영향을 주지 못하였으나, epoxide hydrolase는 positive control로 사용한 ascorbic acid에 미치지 못하지만, bromobenzene 처리군 보다 39% 효소활성을 회복 시켰다. 따라서, (+)-catechin은 간독성 물질을 무독화시키는 epoxide hydrolase의 활성을 회복시켜 간보호 활성을 나타냄을 알 수 있었으며, 해당화에서 분리한 사포닌 성분인 rosamultin도 이효소의 활성을 증가시킴으로 인해 보호활성을 나타내는 것으로 보고된바 있다.

• 한국식품과학회지
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• 제32권5호
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• pp.1179-1185
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• 2000

멸치액젓의 용매분획을 $50^{\circ}C$에서 10일동안 항온실험한 경우 각 용매분획의 건조중량 10 ${\mu}g$ 또는 20 ${\mu}g$이 5 mg의 linoleic acid의 자동산화를 저해하는 것으로 나타났으며, 용매분획 중 부탄올분획 및 수용액분획이 염분을 함유하고 있었고 염분의 함량은 각각 12.2% 및 21.1%이었다. 흰쥐에게 간장독성을 유발시킨다고 알려진 bromobenzene을 주사하고 간조직을 분리하여 in vitro에서 멸치액젓이 간균질액의 지질과산화물 생성에 미치는 효과를 관찰한 결과, 모든 용매분획들이 3 mg/mL 또는 1 mg/mL 농도에서 지질과산화물 생성을 강하게 감소시켰으며 수용액분획의 경우 0.3 mg/mL 농도에서도 활성을 가지는 것으로 나타났다. 간조직의 지질과산화반응에 관여하는 것으로 알려진 효소들 중 aniline hydroxylase, aminopyrine N-demethylase, xanthine oxidase 및 aldehyde oxidase 활성에 대한 멸치액젓의 효과를 관찰한 결과, 간세포 마이크로솜 분획에 존재하는 aniline hydroxylase 및 aminopyrine N-demethylase의 활성을 모든 용매분획이 저해하였다. 즉, 3 mg/mL 농도에서는 거의 정상쥐의 효소활성 수준까지 회복시켰고, 0.3 mg/mL의 농도에서도 유의적인 효과가 나타났으나 세포질 효소인 xanthine oxidase 및 aldehyde oxidase의 활성에 대하여는 저해효과가 나타나지 않았다. 이와 같은 결과로부터, bromobenzene-유도 간지질의 과산화물 생성과정에 있어서 멸치액젓의 항산화물질들은 free radical을 포획하여 지질과산화물 생성을 억제하는 것이 아니라 epoxide생성단계를 저해하여 과산화지질 생성을 억제하는 것으로 사료된다.

• 한국식품영양과학회지
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• 제23권6호
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• pp.894-898
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• 1994

To evaluate an effect of dietary protein on the liver damage, the bromobenzene was intraperitoneally injected to the rats fed a low or high protein diet and then the liver weight per body weight and serum levels of alanine aminotransferase (ALT) activities were determined to demonstrate the differences in liver damage between the groups fed low or high protein diet. Hepatic aniline hydroxylase (AH), glutthione (GSH) content and glutathione s-transferase(GST) activity were also determined to clarify causes of liver damage between the two groups. Increases of liver weight per body weight and serum ALT activities were higher in brombenzene treated rats fed low protein diet than those fed high protein diet. The increasing rate of hepatic AH activity was higher in bromobenzne-treated rats fed low protein diet than that in those fed high protein diet. Furthermore , hepatic glutathione contents and GST activities in bromobenzene-treated rats were higher in rats fed high protein diet than those fed low protein diet. In case of control group, the heaptic glutathione content and GST activity were also higher in rats fed high protein diet than those fed low protein diet.

• Toxicological Research
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• 제13권4호
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• pp.371-376
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• 1997

To compare the severe liver damage with the slight one on the bromobeazene metabolism in rats, the animal group described as B7 group was induced the stage of slight liver damage with 7 times bromobenzene injection every other day (400 mg/Kg body wt. i.p.), whereas B40 group was induced that of more severe liver damage with bromobeazene 40 times injection as identified with determination of serum levels of alanine aminotransferase(ALT) activity and the histopathological findings. In the present experimental animal model, the decreasing rate of glutathione(GSH) and the increasing rate of glutathione S-transferase activity to the control group were higher in B7 group than B40 group. Furthermore the single dose of bromobenzene was injected to the two groups and sacrificed at 8hr and the hepatic aniline hydroxylase(AH) activity, GSH content and GST activity were determined. The increasing rate of AH activity to the control was lower in B40 group than B7 group and the decreasing rate of GSH to the control was also lower in B40 than B7 group. Moreover, B7 group showed the increased activity of hepatic GST to the control whereas B40 group showed the decrease activity of the enzyme. And Vmax value in GST was more decreased in B40 group than B7 group.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1988년도 학술대회지
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• pp.39-42
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• 1988

이 연구는 mouse liver의 epoxide hydrolase에 대한 인삼 사포닌 분획들의 효과를 검정하기 위해 고안되었다. Epoxide hydrolase는 반응성을 갖는 수많은 epoxide 중간물질들의 독성 및 발암성으로부터 세포를 보호하는데 중요한 역할을 하는 효소들의 한 group이다. Cytosolic epoxide hydrolase 활성도는 저농도의 사포닌 분획 첨가시에는 농도에 비례하여 활성이 증가되었으나, 고농동에서는 반대효과를 나태냈다. 수일간 인삼 사포닌 분획을 처리한 epoxide hydrolase의 활성도는 무처리에 비해 유의하게 증가되었음이 관찰되었다.

• Journal of Ginseng Research
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• 제12권2호
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• pp.173-181
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• 1988

We have studied the mechanism by examing the effect of ginseng on the epoxide hydrolase which is catabolized the reactive intermetabolite of bromobenzene, and bromobenzene-induced hepatotoxicity. It was observed that ginseng saponin fraction protects against bromo benzene-induced hepatotoxicity in mice as evidenced 1. increased the epoxide hydrolase activity, 2. lower serum transaminase activity, 3. decreased the formation of lipid peroxide. These results suggested that the inducing effect of ginseng on the epoxide hydrolase is believed to be a possible detoxication mechanism for the bromobenzene toxicity in mice.

• 생약학회지
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• 제31권2호
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• pp.228-234
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• 2000

Effects of Houttuynia cordata on the level of lipid peroxide and the enzyme activities of the liver were investigated in bromobenzene-induced rats. Lipid peroxide content in liver was increased by bromobenzene. It was decreased when the methanol extract from the aerial parts of H. cordata was treated to the rat. The methanol extract reduced the activities of aminopyrine N-demethylase and aniline hydroxylase that increased by bromobenzene, however did not affect glutathione S-transferase activity. The methanol extract recovered the activity of epoxide hydrolase activity that decreased significantly by bromobenzene. We suggest that under our experimental conditions the extract might play an important play in the prevention of hepatotoxicity by reduction of aminopyrine N-demethylase and aniline hydroxylase activities as well as enhancement of epoxide hydrolase activity. Six phenolic compounds have been isolated from H. cordata and identified by means of spectral analysis as protocatechuic acid, quercetin, apigenin, afzelin, hyperoside and quercitrin.

• 동의생리병리학회지
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• 제17권5호
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• pp.1177-1181
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• 2003

Chinese cabbage is a vegetable of Cruciferous family. It was usually consumed as Kimchi. It was known to have amount of vitamin c. Recently the trend for the development of functional food combined with oriental herbs. For this aim the study was performed to evaluate the hepatoprotective effect via antioxidant activity of leaf and root of Sanchon Chinese cabbage(Brassica campetris L.) comparatively. The methanol extracts of Chinese cabbage were tested for investigating the effects on the formation of lipid peroxide and the activities of free radical generating enzyme in vitro in bromobenzene-treated rats. The methanol extracts of chinese cabbage reduced bromobenzene-induced hepatic lipid peroxidation and inhibited the activity of xanthine oxidase. The methanol extracts of chinese cabbage did not activate amionopyrine N-demethylase, aniline hydroxylase and glutathione S-transferase. Epoxide hydrolase activity was decreased by bromobenzene, which was restored by pretreatment of the methanol extracts of chinese cabbage. The results suggest that the methanol extracts of Chinese cabbage is reduced by enhancing the activity of epoxide hydrolase.

• Fisheries and Aquatic Sciences
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• 제20권9호
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• pp.22.1-22.6
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• 2017

The antihyperlipidemic and antioxidant activities of dietary supplementation of sea tangle from Goseong and the alginate-free residue of sea tangle were investigated in Sprague Dawley rats treated with a high-fat diet, streptozotocin, poloxamer 407, and bromobenzene. The alginate-free residue of Goseong sea tangle induced a significant reduction in triglycerides and total cholesterol levels, as well as a significant increase in high-density lipoprotein cholesterol levels. Alginate-free Goseong sea tangle residue reduced the activities of the phase I enzymes aminopyrine N-demethylase and aniline hydroxylase, which had been increased by intraperitoneal injection of bromobenzene. Pretreatment with Goseong sea tangle residue prevented a bromobenzene-induced decrease in epoxide hydrolase activity. Bromobenzene reduced hepatic glutathione content and increased hepatic lipid peroxide levels. Pretreatment with alginate-free Goseong sea tangle residue prevented lipid peroxidation induced by bromobenzene, but pretreatment with Goseong sea tangle did not. These results suggest that Goseong sea tangle residue exerted antihyperlipidemic and antioxidant activities that were higher than those induced by alginate-containing sea tangle. Therefore, the alginate-free residue may contain physiologically unknown active components, other than alginic acid, which may potentially be used to prevent hyperlipidemic atherosclerosis.