• 제목/요약/키워드: Bonding structure

검색결과 973건 처리시간 0.028초

A Study on the Inhibition of 2-deoxy-D-Glucose Transport of the Endogenous Glucose Transporters in Spodoptera frugiperda Clone 21-AE Cells by Using Hexoses

  • Lee Chong-Kee
    • 대한의생명과학회지
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    • 제11권4호
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    • pp.487-492
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    • 2005
  • The baculovirus/insect cell expression system is of great value in the study of structure-function relationships in mammalian glucose-transport proteins by site-directed mutagenesis and for the large-scale production of these proteins for mechanistic and biochemical studies. Spodoptera frugiperda Clone 21 (Sf2l) cells grow well on TC-100 medium that contains $0.1\%$ D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transporters. However, very little is known about the properties of the endogenous sugar transporter(s) in Sf2l cells, although a saturable transport system for hexose uptake has been previously revealed in the Sf cells. In order to further examine the substrate and inhibitor recognition properties of the Sf2l cell transporter, the ability of hexoses to inhibit 2-deoxy-D-glucose (2dGlc) transport was investigated by measuring inhibition constants $(K_i)$. The $K_i's$ for reversible inhibitors were determined from plots of uptake versus inhibitor concentration. Transport was effectively inhibited by D-mannose and D-glucose. Of the hexoses tested, L-glucose had the least effect on 2dGlc transport in the Sf2l cells, indicating that the transport is stereoselective. Unlike the human HepG2 type glucose transport system, D-mannose had a somewhat greater affinity for the Sf2l cell transporter than D-glucose, implying that the hydroxyl group at the C-2 position is not necessary for strong binding. However, epimerization at the C-4 position of D-glucose (D-galactose) resulted in a dramatic decrease in affinity of the hexose for the Sf2l cell transporter. Such a lowering of affinity might be the result of the involvement of the C-4 hydroxyl in hydrogen bonding. It is therefore suggested that Sf2l cells were found to contain an endogenous sugar transport activity that in several aspects resembles the human HepG2 type glucose transporter, although the insect and human transporters do differ in their affinity for cytochalasin B.

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한국형 전술차량 후방밴 강성·강도 보강을 통한 내구성 향상에 관한 연구 (A Study on the way to improve the rear van's durability of Korean Light Tactical Vehicle)

  • 박진원;김두호;송부근;김선진;윤상진
    • 품질경영학회지
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    • 제46권2호
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    • pp.269-282
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    • 2018
  • Purpose: The purpose of this study was to improve the FRP Van's durability by analyzing the problematic parameters, redesigning the rear van, and verifying the design drafts using the CAE analysis & Rig test. Methods: The collected data through the government quality inspection and field spot check were thoroughly analyzed through the characteristics diagram and the improvement suggestions were verified by performing CAE analysis, like the dynamic stiffness, Torsional stiffness, open/close condition's strength, Full car durability and Carrying out the actual test. Results: The results of this study are as follows; The output of CAE analysis shows that improvement suggestions have considerable effects on the reinforcement of FRP structure, and the actual torsion and open/close condition durability test prove that rear van may have durable life which is equivalent to vehicle life cycle. Conclusion: The structural weakness of KLTV's FRP rear van was overcome by applying the stiffener in rear van and changing the bonding method of each FRP pieces. That suggestions were proved using CAE analysis and Rig test.

초음파 분무 열 분해법을 통해 제조된 불소 도핑 된 주석 산화물 나노 입자의 전기화학적 특성 (Electrochemical Properties of Fluorine-Doped Tin Oxide Nanoparticles Using Ultrasonic Spray Pyrolysis)

  • 이도영;이정욱;안건형;류도형;안효진
    • 한국재료학회지
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    • 제26권5호
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    • pp.258-265
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    • 2016
  • Fluorine-doped tin oxide (FTO) nanoparticles have been successfully synthesized using ultrasonic spray pyrolysis. The morphologies, crystal structures, chemical bonding states, and electrochemical properties of the nanoparticles are investigated. The FTO nanoparticles show uniform morphology and size distribution in the range of 6-10 nm. The FTO nanoparticles exhibit excellent electrochemical performance with high discharge specific capacity and good cycling stability ($620mAhg^{-1}$ capacity retention up to 50 cycles), as well as excellent high-rate performance ($250mAhg^{-1}$ at $700mAg^{-1}$) compared to that of commercial $SnO_2$. The improved electrochemical performance can be explained by two main effects. First, the excellent cycling stability with high discharge capacity is attributed to the nano-sized FTO particles, which are related to the increased electrochemical active area between the electrode and electrolyte. Second, the superb high-rate performance and the excellent cycling stability are ascribed to the increased electrical conductivity, which results from the introduction of fluorine doping in $SnO_2$. This noble electrode structure can provide powerful potential anode materials for high-performance lithiumion batteries.

낙뢰로 인한 전기화재의 현장조사기법 연구 (A Study on Investigation Method of the Electric Fire Scene Caused by Lightning)

  • 송재용;사승훈;남정우;김진표;박남규
    • 한국화재소방학회논문지
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    • 제25권1호
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    • pp.50-56
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    • 2011
  • 최근 지구온난화에 의한 영향으로 낙뢰의 발생빈도가 증가하는 추세이고, 낙뢰에 수반되는 뇌격전류의 강도 또한 강해지고 있는 추세이다. 2008년 기준 국내의 낙뢰 발생 빈도는 56만여 건 정도가 발생하였으며, 낙뢰로 인한 인명피해 사고 및 건축구조물의 피해는 나날이 증가하고 있다. 특히, 낙뢰로 인한 전기화재는 낙뢰 발생에 따른 대지전위 상승으로 접지선과 전원선간 절연파괴 과정에서 발생되며, 현장조사 결과, 낙뢰 발생 지역에 인접한 지역에서 동시다발적으로 전기적 피해가 발생하였다. 낙뢰에 대한 피해를 방지하기 위해서는 낙뢰 보호 장치의 설치 및 등전위 접지 시행 등의 방법이 요구된다. 또한 낙뢰로 인한 화재현장 조사에 있어서는 기상청에서 제공되는 낙뢰 발생 기록 및 화재현장과 인접한 지역에서 동시다발적인 전기적 특이점 형성 여부에 대한 검토가 필요하다.

ICP Source를 이용한 저온 증착 a-SiNx:H 특성 평가 (Low Temperature Deposition a-SiNx:H Using ICP Source)

  • 강성칠;이동혁;소현욱;장진녕;홍문표;권광호
    • 한국전기전자재료학회논문지
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    • 제24권7호
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    • pp.532-536
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    • 2011
  • The silicon nitride films were prepared by chemical vapor deposition using inductively coupled plasma. During the deposition, the substrate was heated at $150^{\circ}C$ and power 1,000 W. To evolution low temperature manufacture, we have studied the role of source gases, $SiH_4$, $NH_3$, $N_2$, and $H_2$, to produce Si-N and N-H bond in a-SiNx:H film growth. $SiH_4$, $NH_3$, and $N_2$ flow rate fixed at 100, 10, and 10 sccm, $H_2$ flow rate varied from 0 to 10 sccm by small scale. To get the electrical characteristics, we makes MIM structure, and analysis surface bonding state. Experimental data show that Si-N and N-H bond is increased and hence electrical characteristics is showed 3 MV/cm breakdown-voltage, and leakage-current $10^{-7}\;A/cm^2$.

절연박막에서 유전상수의 보상에 관한 연구 (Study on the Compensation of Dielectric Constant in Dielectric Materials)

  • 오데레사
    • 한국진공학회지
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    • 제18권6호
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    • pp.435-439
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    • 2009
  • SiOC 박막의 유전상수가 낮아지는 원인에 대하여 굴절계수와 C-V 측정법을 이용하여 얻은 파라미터를 사용하여 연구되었다. SiOC 박막은 해리된 가스의 재결합을 통하여 이온결합에 의해서 형성된다. 전통적으로 유전상수는 굴절률의 제곱으로 얻을 수 있거나 혹은 금속/절연체/실리콘 구조에서 C-V 측정법을 이용하여 얻어진다. 유전상수는 이온과 전자 성분으로 이루어졌다. 그래서 이온과 전자성분을 포함한 SiOC 박막의 평균적인 유전상수에 대하여 조사되었다. 유전상수는 열처리 후 감소되었다. 증착한 박막은 대부분이 이온효과에 의하여 유전상수가 구성되는 경향성이 있으며, 반대로 열처리한 박막에서는 전자에 의한 효과가 컸다. 왜냐하면, 이온의 효과가 열처리에 의해 감소되기 때문이다. 결과적으로 열처리 공정을 통하여 SiOC 박막의 이온효과는 감소하고 전자의 효과는 증가된다는 것을 확인하였다.

입자 결합 및 파쇄 형태에 따른 전단거동 특성 (Characteristics of Shear Behavior According to State of Particle Bonding and Crushing)

  • 정선아;김은경;이동석;이석원
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2010년도 춘계 학술발표회
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    • pp.314-323
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    • 2010
  • Recently, granular soils having a large particle size are frequently used as a filling material in the construction of foundation, harbor, dam, and so on. The shear behavior of this granular soil plays a key role in the stability of structures. For example, soil particle crushing occurring at the interface between structure and soil and/or within soil mass can cause the disturbance of ground characteristics and consequently induce an issues in respect of stability of structures. In order to investigate the shear behavior according to an existence and nonexistence of particle crushing, numerical analyses were conducted by using the DEM(Discrete Element Method)-based software program PFC(Particle Flow Code). Using the crushing model and non-crushing model which were created in this study, numerical analyses of ring shear test were conducted and their results were analyzed and compared. In general, landslide and slope stability are accompanied by a large displacement and consequently not only a peak strength but also a residual strength are very important in the analysis of landslide and slope stability. However the direct shear test which has been commonly used in the determination of shear strength parameters has a limitation on displacement therefore the residual strength parameters can not be obtained. The characteristics of residual shear behavior were investigated through the numerical analyses in this study.

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초임계 암모니아를 이용한 p-Phenylenediamine(PPD) 합성 및 특성연구 (Synthesis of p-Phenylenediamine (PPD) using Supercritical Ammonia)

  • 조항규;임종성
    • Korean Chemical Engineering Research
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    • 제53권1호
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    • pp.53-56
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    • 2015
  • 초임계 암모니아 분위기와 CuI 촉매 하에서 p-Diiodobenzene(PDIB)를 아민화 반응시켜 p-Phenylenediamine(PPD)를 합성하는 방법을 연구하였다. 본 연구에서는 여러 가지 공정변수들이 PPD 생성 수율에 미치는 영향을 알아보기 위하여 반응 온도, 암모니아 초기 주입 양에 따른 반응 압력, 촉매의 유무 및 촉매 주입량, 반응 시간 등을 변화시키면서 이에 따른 PPD 수율 변화를 GC 분석을 통하여 조사하였다. 그 결과, 무촉매 반응 시에는 PPD가 전혀 생성되지 않음을 알 수 있었으며, 반응온도, 반응 압력, 촉매 주입량 및 반응시간이 증가함에 따라 PPD 생성 수율이 증가하는 것을 확인할 수 있었다 단, 반응온도의 경우 $250^{\circ}C$ 이상에서는 열분해에 의해 PPD가 감소하여 $200^{\circ}C$가 최적의 온도임을 알 수 있었다. 또한, FT-IR과 $^1H$-NMR 분석을 통하여 아민기의 결합 특성과 PPD의 구조를 확인하였다.

도재주조용 합금에 있어서 알루미륨 첨가에 따른 metal-ceramic과의 결합력 증진에 관한 연구 (A Study on Improvement of Metal-Ceramic Bonding Strength by Addition of Aluminum to Casting Metal Alloy)

  • 이재원;민병국;한민수
    • 대한치과기공학회지
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    • 제23권2호
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    • pp.161-170
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    • 2002
  • The Purpose of this study was to investigate the chemically improvement of metal-ceramics bond strength in the course of recasting Ni-Cr metal composite system with 10wt.%, 20wt.% and 30wt.% aluminum respectively. We have tested the bond strength, micro-structure, chemical composition of each metal composites and metal- ceramic bond interfaces by 3-point bending strength tester, SEM and EDS. We have made the conclusions through this study as follow: 1. The most suitable amount of aluminum to the Ni-Cr metal composite recasting is 20wt. % for improving metal-ceramics bond strength with debonding strength value of 49.54 kgf/mm2. 2. The aluminum must be changed to small spread alumina like phases and second aluminum-metal composites phases in the morphology of Ni-Cr metal composite system by adding during it's casting. These second phases have inclined functional oxide phases mixed with metal elements and they must take roll to improvement of metal-ceramics bond strength. 3. In the case of 30wt.% aluminum appended to Ni-Cr metal composite system, an excess of second inclined functional oxide phases produce cracks and spalling of them apart from it's base material. It must be a important factor of reduction of metal-ceramics bond strength.

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Ti:LiN$bO_3$ 광변조기 소자의 패키징 및 전기.광학적 특성 (The study of the packaging for Ti:LiN$bO_3$optical modulator device and its electrical and optical characteristics)

  • 윤형도;김성구;이한영;윤대원
    • 전자공학회논문지D
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    • 제35D권6호
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    • pp.72-78
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    • 1998
  • Ti:LiNbO$_3$ 광도파로위에 CPW 전극 구조를한 광변조기 내부칩을 제작한 후 일련공정에 따라 패키징 작업을 하였고 소자의 전기적특성과 광학적특성을 측정하였다. 광변조기 패키징을 위하여 페룰, 보조용 LN블럭 및 글래스, 진동 및 흡수용패드, 알루미나피더의 부분품을 사용하였으며 피그테일링, Au 와이어본딩, 에폭싱, SMA커넥팅, 실링 작업을 수행하였다. 전기적 특성에서 S/sub 21/은 -3 dB 점에서 9.8GHz의 값을, S/sub 11/은 14.4 GHz 대에서 -8.9 dB 값을 나타내었다. 광학적 특성은 제작된 광도파로가 1550nm 파장대에서 단일모드를 만족하였고, 패키지후 소자의 삽입손실은 실온에서 4.3 dB 이였으며, 소자를 온도감압챔버에 넣은후 5∼45℃까지 온도변화를 주었을 때 4.3∼6.4 dB의 삽입손실을 보였다. E-O bandwidth 응답은 3dB점에서 7.8GHz를 나타내어 10Gbps급 광통신시스템에 응용할수 있음을 확인하였다.

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