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Determination of Tiapride in Human Plasma Using Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry

  • Moon, Ya;Paek, In-Bok;Kim, Hui-Hyun;Ji, Hye-Young;Lee, Hye-Won;Park, Hyoung-Geun;Lee, Hye-Suk
    • Archives of Pharmacal Research
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    • v.27 no.9
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    • pp.901-905
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    • 2004
  • A rapid, sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric(HILIC-MS/MS) method for the determination of tiapride in human plasma was developed. Tiapride and internal standard, metoclopramide were extracted from human plasma with dichloromethane at basic pH and analyzed on an Atlantis HILIC silica column with the mobile phase of acetonitrile-ammonium formate (190 mM, pH 3.0) (94:6, v/v). The ana-Iytes were detected using an electrospray ionization tandem mass spectrometry in the multi-ple-reaction-monitoring mode. The standard curve was linear (r=0.999) over the concentration range of 1.00-200 ng/mL. The coefficient of variation and relative error for intra- and inter-assay at three QC levels were 6.4∼8.8% and -2.0∼3.6%, respectively. The recoveries of tiapride ranged from 96.3 to 97.4%, with that of metoclopramide (internal standard) being 94.2%. The lower limit of quantification for tiapride was 1.00 ng/mL using 1 00 $\mu$L of plasma sample.

An Unrecorded Alien Species in Korea: Phalaris paradoxa L. (Poaceae) (한국 미기록 외래식물: 날개카나리새풀(벼과))

  • Ryu, Tae-bok;Lee, Seung-eun;Kim, Deokki;Choi, Dong-hee;Kim, Nam-young
    • Korean Journal of Plant Resources
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    • v.31 no.1
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    • pp.52-55
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    • 2018
  • An unrecorded alien species plant, Phalaris paradoxa L. (Poaceae) was found in Andeok-myeon, Seogwipo-si, Jeju island, Korea. It is native to the Mediterranean region of Europe, and it is currently found worldwide. Phalaris paradoxa is distinguishable from related taxa (P. arundinacea, P. canariensis, P. minor and P. arundinacea var. picta) in Korea by the following combination of characterstics: spikelets in clusters (the upper central fertile spikelet surrounded by six lower sterile spikelets), wing of the keel. This taxon was named 'Nal-gae-ca-na-ri-sae-pul' in Korean based on its character. We provide its description, illustrations, photographs and a key of related taxa in Korea.

Quality Characteristics of Soy Sauces by Various Manufacturing Methods (간장의 제조방법에 따른 품질 특성 비교 연구)

  • Choi, Ji-Mi;Lee, Chun-Bok;Kim, Hak-Seon
    • Culinary science and hospitality research
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    • v.22 no.2
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    • pp.57-65
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    • 2016
  • This study aimed to evaluate quality characteristics of soy sauce by various manufacturing methods. We examined color values, contents of saccharide and free amino acid. Regarding color values, brewed soy sauce showed lower level of L-values than traditional soy sauce, and the L-value was increased with time dependent manner in traditional soy sauce. The one year old traditional soy sauce exhibited the lowest a-value whereas the three years old traditional soy sauce showed the highest b-value (+4.27). The content of the bitter and savory taste amino acids was the highest in commercial soy sauce with the values of 28.98% and 18.93%, respectively. In addition, traditional soy sauce contained more GABA than brewed soy sauce.

Fuzzy M/M/l/K Queueing Network Model for Performance Evaluation of Network System (네트워크 시스템의 성능평가를 위한 퍼지 M/M/l/K 큐잉네트워크모델)

  • Choo, Bong-Jo;Jo, Jung-Bok;Woo, Chong-Ho
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.38 no.4
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    • pp.1-9
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    • 2001
  • In this paper, we propose Fuzzy M/M/1/K queueing network model which has derived by appling the fuzzy set theory to M/M/l/K queueing network model in which has single server and system capacity K. When the arriving rate of input job and the servicing rate of a server arc represented as the linguistic attributes, the system analysis can be performed by using this model. The major evaluation measures of system such as the average number of jobs existing in the system, the average number of jobs into system, and the average spending time of job in system etc. are derived for the evaluation of system. Computer simulation was performed for verifying the effectiveness of these result equations. In which the various fuzzy arriving rates and fuzzy servicing rates according to varying the system capacity K were given for the system evaluation. We verified that the results of simulation are in accord with the expected evaluations in the proposed fuzzy model.

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Effects of Light Source, Plant Growth Regulators (GA, BA, ABA) and Sodium Hypochlorite on 'Grand Rapid' Lettuce (Lactuca Sativa L.) Seed Germination (광질(光質), 생장조절물질(生長調節物質) (GA, BA, ABA) 및 Sodium Hypochlorite의 처리가 상치 (Lactuca sativa L.) 종자의 발아(發芽)에 미치는 영향(影響))

  • Lee, Young Bok;Kim, Young Rae
    • Korean Journal of Agricultural Science
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    • v.10 no.2
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    • pp.242-248
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    • 1983
  • The effects of light source, plant growth regulators (GA, BA, ABA), and sodium hypochlorite (4% Cl, SH) on germination of 'G rand Rapid' lettuce (Lactuca sativa L.) seed were studied. Seed did not germinate under the conditions of dark and blue light. The treatment of GA or BA did not promote on germination under dark or blue light. The germination percentage under red light was very high, it was similar to the condition of white light. ABA inhibited lettuce seed germination. Although ABA was treated, BA 10ppm treatment was moderately effective under white light condition and BA 10ppm or 50ppm combined with GA 50ppm were also effective on seed germination under dark condition. Presoaking in sodium hypochlorite solution induced germination of lettuce seed in any condition of dark or white light. The optimum presoaking time in sodium hypochlorite solution was twenty min.

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Effect of Drought Stress on Carbohydrate Composition and Concentration in White Clover

  • Kim, Tae-Hwan;Lee, Bok-Rye;Jung, Woo-Jin;Kim, Dae-Hyun;Kim, Kil-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.1
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    • pp.48-53
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    • 2002
  • To investigate the changes in the composition and pool size of carbohydrates under drought stress, white clover (Triforium repens L.) were exposed to -0.04 Mpa(well-watered, control) or to -0.12 Mpa (drought-stressed) of soil water potential during 28 days. Dry weight of leaves in drought-stressed plants was remarkably decreased by 45% within 14 days and 74% within 28 days compared to those of the control. Glucose concentration in drought-stressed plants was increased, while that of control was slightly decreased or remained at same level throughout experimental period. Fructose and sucrose concentrations in leaves were not significantly changed for drought-stressed plants, but those of the control were significantly decreased on plant after 14 days. Fructose and sucrose concentrations in stolon of control plants were sharply decreased, while that of drought-stressed plants was less varied. Those concentrations in roots were generally increased in drought-stressed plants. The concentration of total soluble sugars at 28 day was 438.0 and 632.6 mg $g^{-l}$ dwt. in control and drought stressed plants, respectively. Starch concentration of stolon and roots of control plants was significantly increased to 2.0 and 1.4 times of initial level, respectively, whereas those of drought stressed plants was nearly same level or slightly decreased compared to initial level.l.

The Corrosion Effect of the Water Pipelines in Buildings according to Drinking Water Quality (수돗물 수질에 따른 옥내급수관 부식에 미치는 영향분석)

  • Yu, Soon-Ju;Park, Su-Jeong;Ahn, Kyung-Hee;Kim, Hyun-Gu;Kim, Chang-Soo;Jung, Il-Rock;Park, Young-Bok
    • Journal of Korean Society on Water Environment
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    • v.24 no.6
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    • pp.701-708
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    • 2008
  • As a countermeasure for reduction of corrosion in the delivery and distribution pipes used for tap water, materials for the pipelines in-houses and the effect of water quality on corrosivity of water pipelines were investigated in the distribution system of Han river. As the corrosion index at 6 water purification facilities of Han river, average Langelier Saturation Index (LI) of raw and finished water were -1.0 and -1.4 respectively and average Larson Index (LR) were 9.5 and 9.9, respectively. And also corrosion potential showed corrosivity in finished water (-431~-462 mV) as well as raw water (-426~-447 mV). This results indicate that tap water quality of han river have corrosivity. To understand the corrosivity effect in pipe material used for premise distribution system, water quality of stagnant tap water and tap water were analyzed and the differences between them were calculated. The difference concentration of iron, copper and zinc were $12.9{\mu}g/L$, $31.0{\mu}g/L$ and $45.0{\mu}g/L$ respectively in galvanized steel pipe for use more than 15 years and showed highest concentration. As a result, the control to corrosivity in the water pipelines, corrosivity control treatment in the water purification system can be applied. In advance it is necessary to monitor corrosivity of water quality using corrosive index because corrosivity may differ from the seasonal and regional characteristics and water chemicals dosage. For the future the guideline for corrosion index have to be established.

Super-Resolution Reconstruction Algorithm using MAP estimation and Huber function (MAP 추정법과 Huber 함수를 이용한 초고해상도 영상복원)

  • Jang, Jae-Lyong;Cho, Hyo-Moon;Cho, Sang-Bok
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.46 no.5
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    • pp.39-48
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    • 2009
  • Many super-resolution reconstruction algorithms have been proposed since it was the first proposed in 1984. The spatial domain approach of the super-resolution reconstruction methods is accomplished by mapping the low resolution image pixels into the high resolution image pixels. Generally, a super-resolution reconstruction algorithm by using the spatial domain approach has the noise problem because the low resolution images have different noise component, different PSF, and distortion, etc. In this paper, we proposed the new super-resolution reconstruction method that uses the L1 norm to minimize noise source and also uses the Huber norm to preserve edges of image. The proposed algorithm obtained the higher image quality of the result high resolution image comparing with other algorithms by experiment.

Investigation of Impact Detection Characteristics of Piezoelectric Paint According to Boundary Conditions (구조물의 경계조건에 따른 압전 페인트 센서의 충격검출 특성 평가)

  • Park, Seung-Bok;Han, Dae-Hyun;Kang, Lae-Hyong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.12
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    • pp.1335-1343
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    • 2014
  • Piezoelectric paint can be used to monitor vibrations or impacts occurring in large engineering structures such as ships and airplanes. This study investigated the impact detection characteristics of a piezoelectric paint sensor and possible errors in detecting impacts according to boundary conditions. The piezoelectric paint sensor used in this study was coated on an aluminum plate with four different electrode areas. After the occurrence of the poling process, the output voltages from the paint sensors were obtained when impact occurred in a certain sensor region. The experimental results revealed a large difference in magnitudes between the sensor signal in the impact region and those in the other regions, and this relation was maintained regardless of the changes in the boundary conditions.

The role of matrix modifier and for the determination of cadmium in blood by graphite furnace atomic absorption spectrometry (흑연로 원자흡수분광법에 의한 혈중 카드뮴 분석시 매트릭스 개선제(matrix modifier)의 역할)

  • Yoo, Kwang-Sik;Kim, Chang-Bok;Kwon, Jin-Kee
    • Analytical Science and Technology
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    • v.8 no.1
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    • pp.9-16
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    • 1995
  • The low concentration of cadmium in the whole-blood was determined by graphite furnace atomic absorption spectrometry(GFAAS) after the sample was diluted five-fold by 1% Triton X-100, 2% $(NH_4)_2HPO_4$ as matrix modifier and pyrocoated graphite tube with L'vov platform was tried remove the interferences of blood matrix and reduce the loss of volatility of cadmium at higher ashing temperature($600^{\circ}C$). The criteria for evaluating the accuracy and precision of this analysis was confirmed by analysis of interlaboratory comparison(Japan) and NIST SRM No. 909(Cd in Serum). The limit of the determination for cadium was 0.1ng/ml and the relative standard deviation(RSD) at 1.0ng/ml level was about 10% for the GFAAS.

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