Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Determination of Tiapride in Human Plasma Using Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry

  • Moon, Ya (Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Phytofermentation Research Center, Wonkwang University) ;
  • Paek, In-Bok (Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Phytofermentation Research Center, Wonkwang University) ;
  • Kim, Hui-Hyun (Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Phytofermentation Research Center, Wonkwang University) ;
  • Ji, Hye-Young (Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Phytofermentation Research Center, Wonkwang University) ;
  • Lee, Hye-Won (Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Phytofermentation Research Center, Wonkwang University) ;
  • Park, Hyoung-Geun (Research Laboratories, Dong-A Pharm. Co., Ltd) ;
  • Lee, Hye-Suk (Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy and Phytofermentation Research Center, Wonkwang University)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

A rapid, sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric(HILIC-MS/MS) method for the determination of tiapride in human plasma was developed. Tiapride and internal standard, metoclopramide were extracted from human plasma with dichloromethane at basic pH and analyzed on an Atlantis HILIC silica column with the mobile phase of acetonitrile-ammonium formate (190 mM, pH 3.0) (94：6, v/v). The ana-Iytes were detected using an electrospray ionization tandem mass spectrometry in the multi-ple-reaction-monitoring mode. The standard curve was linear (r＝0.999) over the concentration range of 1.00-200 ng/mL. The coefficient of variation and relative error for intra- and inter-assay at three QC levels were 6.4∼8.8％ and -2.0∼3.6％, respectively. The recoveries of tiapride ranged from 96.3 to 97.4％, with that of metoclopramide (internal standard) being 94.2％. The lower limit of quantification for tiapride was 1.00 ng/mL using 1 00 $\mu$L of plasma sample.

Keywords

References

  1. Aly, F. A., Alarfaj, N. A., and Alwarthan, A. A, Flow-injection chemiluminometric analysis of some benzamides by their sensitizing effect on the cerium-sulphite reaction. Talanta, 54, 715-725 (2001) https://doi.org/10.1016/S0039-9140(01)00320-4
  2. Buna, M., Aaron, J. J., Prognon, P. and Mahuzier, G., Effects of pH and solvent on the fluorescence properties of biomedically important benzamides. Application to determination in drugs and in human urine. Analyst, 121, 1551-1556 (1996) https://doi.org/10.1039/an9962101551
  3. Chiba, R., Ogasawara, A., Kubo, T., Yamazaki, H., Umino, M., and Ishizuka, Y., Direct determination of benzamides in serum by column-switching high-performance liquid chromatography. Anal. Sci., 19,785-789 (2003) https://doi.org/10.2116/analsci.19.785
  4. Guidance for Industry-Bioanalytical Method Validation, US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Veterinary Medicine, May 2001, httpJ/www.fda. gov/cder/guidance/ index.htm
  5. Heun, R., Rosler, M., and Allain, H., Tiapride in gerontopsy-chiatry. Eur. Psychiatry, 16 (suppl 1), 3s-4s (2001)
  6. Kamizono, A., Inotsume, N., Miyamoto, K., Ueda, K., Miyakawa, T., Arimoto, H., and Nakano, M., Determination of sultopride and tiapride in serum by gas chromatography using a surface ionization detector. J. Chromatogr., 567, 113-120 (1991) https://doi.org/10.1016/0378-4347(91)80315-4
  7. Kotzailias, N., Finsterer, J., Aull, S., Eichler, H.-G., Pratscher, B., and Jilma, B., Influence of tiapride on platelet counts in healthy volunteers and patients with movement disorders. Prog. Neuropsychopharmacol. Biol. Psychiatry, 27, 595-599 (2003) https://doi.org/10.1016/S0278-5846(03)00046-0
  8. Naidong, W., Bioanalytical liquid chromatography tandem mass spectrometry methods on underivatized silica columns with aqueous/organic mobile phases. J. Chromatogr. B, 796, 209-224 (2003) https://doi.org/10.1016/j.jchromb.2003.08.026
  9. Naidong, W. and Eerkes, A., Development and validation of a hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the analysis of paroxetine in human plasma. Biomed. Chromatogr., 18, 28-36 (2004) https://doi.org/10.1002/bmc.288
  10. Norman, T. R., James, R. H., and Gregory, M. S., Determination of tiapride in plasma by high-performance liquid chromatography. J. Chromatogr. B, 375, 197-201 (1986) https://doi.org/10.1016/S0378-4347(00)83711-1
  11. Robert, P. H. and Allain, H., Clinical management of agitation in the elderly with tiapride. Eur. Psychiatry, 16 (suppl 1), 42s-47s (2001) https://doi.org/10.1016/S0924-9338(00)00527-7