• Title/Summary/Keyword: Blue pigment

Search Result 183, Processing Time 0.019 seconds

Substitution of Pro206 and Ser86 Residues in the Retinal Binding Pocket of Anabaena Sensory Rhodopsin is Not Sufficient for Proton Pumping Function

  • Choi, Ah-Reum;Kim, So-Young;Yoon, Sa-Ryong;Bae, Ki-Ho;Jung, Kwang-Hwang
    • Journal of Microbiology and Biotechnology
    • /
    • v.17 no.1
    • /
    • pp.138-145
    • /
    • 2007
  • Anabaena sensory rhodopsin is a seven transmembrane protein that uses all-trans/13-cis retinal as a chromophore. About 22 residues in the retinal-binding pocket of microbial rhodopsins are conserved and important to control the quality of absorbing light and the function of ion transport or sensory transduction. The absorption maximum is 550 nm in the presence of all-trans retinal at dark. Here, we mutated Pro206 to Glu or Asp, of which the residue is conserved as Asp among all other microbial rhodopsins, and the absorption maximum and pKa of the proton acceptor group were measured by absorption spectroscopy at various pHs. Anabaena rhodopsin was expressed best in Escherichia coli in the absence of extra leader sequence when exogenous all-trans retinal was added. The wild-type Anabaena rhodopsin showed small absorption maximum changes between pH4 and 11. In addition, Pro206Asp showed 46 nm blue-shift at pH7.0. Pro206Glu or Asp may change the contribution to the electron distribution of the retinal that is involved in the major role of color tuning for this pigment. The critical residue Ser86 (Asp 96 position in bacteriorhodopsin: proton donor) for the pumping activity was replaced with Asp, but it did not change the proton pumping activity of Anabaena rhodopsin.

The Study of Development of Color-Mud for Boryeong Mud Festival by Color Pigment (착색안료를 이용한 보령머드축제용 유색머드의 개발)

  • Shim, Seung-Bo;Oh, Seong-Geun;Hong, Kyung-Hee;Chun, Yong-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.11 no.6
    • /
    • pp.2300-2305
    • /
    • 2010
  • This study was performed about color-contained mud to provide fun and diversity for the event of mud self massage as a core program of Boryeong Mud Festival which has become one of representative festivals in Korea. Adjusting its own lower brightness of mud and based on the primary study performed about the expressivity of yellowish colors, inorganic colors (oxidized steel in red, ultra marine pink, ultra marine blue, chrome oxide green) were added for the development of colored mud in diverse colors on the basis of Korean traditional colors. It had been judged about each expressivity of color by methods of colormeter, naked eyes inspection and feeling of usage, etc. For the color decided, it had been examined about the coloring when it got wet with the cleaning condition so that it can be used for the event of mud self massage as a program of Boryeong Mud Festival to increase diversity of the Festival and also attempted to develope colored mud to show Korean traditional colors.

The Effects of Storage Temperature and pH on Color Change in Garlic Puree (마늘 퓨레의 변색에 관여하는 저장온도와 pH의 영향)

  • 장현세;홍경훈
    • Food Science and Preservation
    • /
    • v.5 no.3
    • /
    • pp.211-216
    • /
    • 1998
  • This study was carried out to how the effects of storage temperature of garlic bulbs, pH and temperature of garlic puree on its color change. Color change was severely appeared in puree made of garlics stored at pH 4.0, 2$^{\circ}C$ and pH 4.0, 25$^{\circ}C$. The color of garlic puree was changed from green to blue during storage. The yellowing occured by the oxidation at high temperature of puree. Garlic puree absorbed the wavelengths of 486nm. 580nm, and 656nm. The precursors of pigments produced by enzymatic reaction were seemed to be the reasons of color change because there was no my change of color in puree when the enzymes including alliinase were inactivated. However, the color changes of puree at low storage temperature should be studied further in the future.

  • PDF

Hydrothermal synthesis of $(Li,Al)MnO_2(OH)_2$:Co compound (수열법에 의한 $(Li,Al)MnO_{2}(OH)_{2}$:Co 화합물의 합성)

  • 최종건;황완인;김판채
    • Journal of the Korean Crystal Growth and Crystal Technology
    • /
    • v.11 no.4
    • /
    • pp.154-159
    • /
    • 2001
  • (Li,Al)$MnO_2(OH)_2$:Co compound was synthesized by hydrothermal method. $MnO_2$, LiOH.$H_2$O, $Co_3O_4$ and $Al(OH)_3$ were used as starting materials and the optimum conditions for synthesis of monolithic (Li,Al)$MnO_2(OH)_2$:Co compound were as follows : reaction temperature; $200^{\circ}C$, reaction time; 3 days, hydrothermal solvent; 3M-KOH solution, reaction apparatus; seesaw type, atomic ratio of Li:Al:Mn;Co = 1:2.1:2.5~2:0.5~1. Monolithic(Li,Al)$MnO_2(HO)_2$:Co compound synthesized in this work had a god crystallinity and excellent color forming effect as a blue pigment compatible with natural mineral. The particles of the synthesized (Li,Al)$MnO_2(OH)_2$:Co compound have hexagonal plate shape with the size of 0.5~1 $\mu\textrm{m}$.

  • PDF

The Effect of Light on Germination of the Major Weed Seeds in Upland Fields (주요(主要) 밭잡초종자(雜草種子)의 발아(發芽)에 미치는 광(光)의 영향(影響))

  • Woo, I.S.;Choi, K.S.;Pyon, J.Y.
    • Korean Journal of Weed Science
    • /
    • v.10 no.4
    • /
    • pp.305-311
    • /
    • 1990
  • Effects of light on germination of major weeds in upland fields were examined. Most weeds showed light positive response to germination at above 25$^{\circ}C$, but germination percentage was very low at lower temperatures. Red light promoted germination of Amaranthus lividus, Portulaca oleracea, and A. retroflexus, but far-red light inhibited germination. Germination resposnse of A. retroflexus, A. lividus, and P. oleracea showed reversible reaction to red/far-red light, and thus it seemed that phytochrome was involoved in seed germination, Echinochloa crusgali and Eleusine indica showed higher germination percentage under the conditions of continuous illumination of red, white, and bule lights, and thus it was estimated that blue light pigment (HIR, high irradiation reaction) related to long time illumination was involved in germination in addition to phytochrome.

  • PDF

Comparative Study on the Pigments Applied on the Wall Paintings of Temple in 18~19C (18~19세기 사찰벽화에 사용된 안료 비교 고찰)

  • Son, Young;Kang, Dai Ill;Lee, Hwa Soo;Lee, Han Hyoung
    • Journal of Conservation Science
    • /
    • v.29 no.4
    • /
    • pp.445-450
    • /
    • 2013
  • This study investigated the properties of the painting materials used in the temple wall paintings of the $18^{th}$ to $19^{th}$ centuries by synthetically comparing the component analysis data on the pigments used in the temple wall paintings. The study subjects analyzed from ED-XRF are the data on the 61 temple wall paintings distributed nationwide. The colors of the wall painting coloring layers are classified into seven categories: white, incanadine, yellow, red, green, blue and black color. No big geographical and temporal differences in the type of the pigments were found in the temple paintings of the late Joseon Dynasty distributed in Gyeongsangdo and Jeollado. On the other hand, there were differences in the use of a color when mixing it with other colors depending on the painted parts.

Scientific Analysis and Conservation of Nectar Ritual Painting in the 16th Century (16세기 감로도의 과학적 조사와 보존)

  • Ahn, Jiyoon;Cheon, Juhyun;Kim, Sooyeon
    • Conservation Science in Museum
    • /
    • v.14
    • /
    • pp.13-22
    • /
    • 2013
  • The Nectar Ritual Painting(Jeung7551) in National Museum of Korea is estimated to have been produced in the 16th century. Ezimagodo who is the head priest of Ruganji in Kyoto, Japan donated this painting in 2010. Overall, damages were serious, such as missing, staining, folding, insects of the face and mounting silk, therefore, it need to conservation treatment. The treatment of conservation in painting was removing surface stains and repairing missing area. It was mounted by following the original form. The analysis result of textile in mounting and support fiber used silk and hemp in microscope. Pigments in paint were divided into six color types. This painting was used that vermilion(cinnabar) and red lead for red color, litharge(massicot) and gold for yellow color, lead white for white color, malachite for green color, azurite for blue color, and carbon black(chinese ink) for black color.

The Stability Appraisement on Cultural Property Material with the Replacing Fumigation Gas of Methyl Bromide (Methyl Bromide를 대체하는 훈증 가스의 문화재 재질 안정성 평가)

  • Kang, Dai-Ill
    • Journal of Conservation Science
    • /
    • v.25 no.3
    • /
    • pp.283-291
    • /
    • 2009
  • Methyl Bromide that was used as fumigation gas turned out to be the substance of destroying the ozone layer. For that reason, at the Montreal Protocol in 1987 the use of methyl bromide was forbidden starting 2005 in the advanced country. Also according to the 2007 Bali Protocolly methyl bromide is expected to be forbidden and therefore the purpose of this study is to find out the effects of substitution fumigation gas (Ethylene Oxide+HFC 134a, Methyl Iodide, Cyanogen and Argon) on the metal(silver, copper and iron), wood(oregon pine), pigment(yellow, red, blue, white and black), textile(hemp, ramie, jute, silk1 and silk2 / indigo, safflower and cork) and paper. After the fumigation test, ethylene oxide+HFC 134a did not have changes in the weight and color of the material itself before and after the experiment. On exterior alteration, color change occurred partly on paper and metal. Also, in most materials color change extent was 0.5 to 1.5 on the average and showed scanty difference. The materials after the fumigation test with methyl iodide did not show weight changes after the test. However, color changes more than 1.0 was shown in most of the materials especially in dyed textile material. In blue pigment, the discoloration on the surface could be seen by naked eyes. Fumigation test with cyanogen gas did not show weight changes and discoloration is more than 1.5 before and after the test. The weight changes of test materials with the argon gas was decreased about 3 to 6%. It can be observed that discoloration on paper was generated. Color changes can be seen on jute dyed with safflower and cork for two weeks with argon gas and the extent was 6.3 and 6.0.

  • PDF

Discrimination between FRC-post and core according to the color difference (색상차이에 따른 FRC-포스트와 코어 사이의 구별)

  • Kim, Jou-Hwe;Kim, Jin-Woo;Cho, Kyung-Mo;Park, Se-Hee
    • Journal of Dental Rehabilitation and Applied Science
    • /
    • v.31 no.2
    • /
    • pp.75-85
    • /
    • 2015
  • Purpose: The purpose of this study is to evaluate which FRC-posts were more distinguishable from core. Materials and Methods: Nine extracted single-rooted mandibular premolars with similar lengths (${\pm}0.5 mm$) and widths (${\pm}0.5 mm$) were endodontically treated and obturated. One specimen which the three roots were embedded in a cold mounting resin was made. 3 specimens were made by this method. Post spaces were prepared for the following post system: FRC $Postec^{(R)}$ Plus, MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$, $Snowpost^{(R)}$. After three different posts were placed in the roots of a specimen, each three specimens received a direct core build-up: $Luxacore^{(R)}$ Dual A3, $Luxacore^{(R)}$ Dual blue, $Filtek^{TM}$ Z350 A1E. Digital images were taken of the post and core with and without air-blowing. We asked to fifty dentists and fifty dental college students which post was more clearly discriminated from the core. Results: In surveys, when core was $Luxacore^{(R)}$ Dual blue, among three types of posts people more easily discriminated the $Snowpost^{(R)}$ from core. When core was $Luxacore^{(R)}$ Dual A3, among three types of posts people similarly more easily discriminated $Snowpost^{(R)}$ from core. When core was $Filtek^{TM}$ Z350 A1E, among three types of posts people more easily discriminated distinguished MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$ post from core. People more easily distinguished MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$ post from core when temperature was lowered by air-blowing. Conclusion: Ability to discriminate between FRC-post and core is different according to color contrast. MACRO-$LOCK^{TM}$ POST $ILLUSION^{TM}$ $XRO^{(R)}$ posts are more discriminable when temperature is lowered by air-blowing.

Evaluation of the Color-change and Stability of Hoecheong (Smalt) Pigments When Exposed to Airborne Environmental Pollutants (회청 안료의 보존 환경에 따른 안정성 평가)

  • PARK, Juhyun;LEE, Sunmyung;KIM, Myoungnam
    • Korean Journal of Heritage: History & Science
    • /
    • v.54 no.4
    • /
    • pp.22-35
    • /
    • 2021
  • Recently, as the climate changes rapidly and the prevalence of airborne fine particulate matter increases, the pattern of pollutants in the atmospheric environment is also changing. Therefore, the importance of studying the stability of pigments used in colored cultural properties is emerging. Hoecheong is an inorganic blue glass pigment called smalt; it is made by using cobalt as a coloring element in potash glass, and was widely used in colored cultural assets, such as murals and paintings. In this study, we collected three other hoecheong pigments to analyze their properties. The percentage of Co and K contained are different according to the manufacturer, and the smalt-3 sample has a lower cobalt content (15.1 wt.%) and higher potassium content (29.6 wt.%). After this analysis, colored specimens were prepared. Prepared specimens were exposed to ultra-violet rays, CO2/NO2, and NaCl, which are known to have the greatest influence on the stability of pigments. We found that factors affecting the color stability were NO2 gas, ultra-violet rays, and water-soluble salts (NaCl). Among them, NO2 has the most severe impact on color change of the pigments. Results of the component analysis showed that the color change depends on the potassium and cobalt content of the hoecheong pigment. Among the specimens, smalt-3 showed the most vulnerability after exposure to NO2 gas and water-soluble salts. Pigment film stability is affected by watersoluble salts, giving rise not only to color change, but also weakening the physical properties of the film. However, there was no significant change in composition and color after exposure to CO2 gas. In conclusion, we found that hoecheong pigments underwent color change and increased instability of the coating film when exposed to any of the atmospheric environmental factors used in this study, except for CO2.