• ALGAE
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• v.37 no.2
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• pp.163-174
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• 2022

Light-emitting-diodes (LEDs) are a lighting source useful for the precise evaluation of light quality effect on biological systems. Despite the importance of light spectra on the regeneration of land plant protoplasts ("naked cells"), this factor has not been tested yet on protoplasts from multicellular algae. This study reports on the effects of pure primary colors (red, blue, and green), dichromatic (red plus blue, RB, 1 : 2) and white LEDs on protoplast regeneration from male and female Undaria pinnatifida gametophytes. We also evaluated the effect of different light spectra on pigment composition (chlorophyll a, chlorophyll c, and fucoxanthine), and the light intensities under the best condition on the regeneration process. In the early stages, blue or RB LEDs increased the percentage of dividing female protoplasts, whereas red, blue, and RB LEDs enhanced that of dividing male protoplasts. In the later stages, RB LEDs showed a positive effect only on the percentage of multiple rhizoid-like protrusions (male gametophyte). They also increased the final area of both regenerated gametophytes. The LEDs did not affect pigment composition in female gametophytes. In male gametophytes, in contrast, they reduced chlorophyll c, while blue, RB, and green LEDs decreased fucoxanthin. Under RB LEDs, the optimal light intensity was 80 µmol photons m^-2 s^-1 for female gametophytes and 40 to 60 µmol photons m^-2 s^-1 for male gametophytes. Our results suggest that dichromatic LED illumination (red-blue) improves regeneration of U. pinnatifida gametophyte-isolated protoplasts. Thus, dichromatic LEDs might a suitable light source for enhancing protoplast regeneration in brown seaweeds.

• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.413-420
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• 2018

Purpose : We present a novel method for the analysis of blue light applied by the analysis method of David L. with optical experiment and blue light by measuring the transmittance by dividing it by the refractive power in the spectacle lens made by MR8. Methods : The lenses of -8.00D, -7.00D, -6.00D, -5.00D, -4.00D, -3.00D, and 0.00D manufactured by MR8 being sold in the market, were selected. The transmittance was measured at the intervals of 5 nm from 200 to 1000 nm with UV-VIS Spectrophotometers (SolidSpec 3700), and they were in range of the blue light (380 to 500 nm) analyzed by a David L.'s analysis method. Results : All of the MR8 lenses selected for this study almost completely blocked at the UV range. A lens of -8.00D was measured as the lowest transmittance of 59.56% in the blue light area and low values were measured at the blue areas 1 and 2 according to the analysis of David L.In the infrared ray area, the transmittance of all lenses gradually decreased. The average value of the luminous transmittance was 23.67% ~ 26.33% and then gradually decreased from -4.00D. Conclusion : Applying the analysis of David L., a minimum of 41.28% and a maximum of 46.60% were measured at the blue light 1 area and a minimum of 87.30% and a maximum of 97.55% were measured at the blue light 2 area. A minimum of 86.83% and a maximum of 96.55% were measured at the blue light 3 area, and the average was 94%. The luminous transmittance of the -3.00D lens was 26.33%, which was the highest, and that -8.00D was 23.67%, which was the lowest.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.214-219
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• 2013

This study was conducted to examine the effect of LED light quality and treatment time on the growth and flowering in potted plants of Impatiens (Impatiens hawkerihybrid). Plant height of Impatiens was enhanced under Blue light, regardless of treatment time. Root length and stem diameter of Impatiens were enhanced by Red light or Blue light. The number of internodes was not influenced by LED light quality. The number of branches of Impatiens was increased under Blue light, but treatment time did not result in statistically significant differences. Leaf area was increased by all LED lights in Impatiens. The number of flower buds and open flowers was decreased by LED light, but days to flowering were reduced by Red light in Impatiens. Chlorophyll and anthocyanin content were not significantly affected by LED light, but anthocyanin content tended to increase by Blue light for 4 h after sunset. Fresh and dry weights were enhanced by Blue light in Impatiens.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.46 no.3
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• pp.59-68
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• 2023

Currently, yellow phosphor of Y3Al5O12:Ce3+ (YAG:Ce) fluorescent material is applied to a 450~480nm blue LED light source to implement a white LED device and it has a simple structure, can obtain sufficient luminance, and is economical. However, in this method, in terms of spectrum analysis, it is difficult to mass-produce white LEDs having the same color coordinates due to color separation cause by the wide wavelength gap between blue and yellow band. There is a disadvantage that it is difficult to control optical properties such as color stability and color rendering. In addition, this method does not emit purple light in the range of 380 to 420nm, so it is white without purple color that can not implement the spectrum of the entire visible light spectrum as like sunlight. Because of this, it is difficult to implement a color rendering index(CRI) of 90 or higher, and natural light characteristics such as sunlight can not be expected. For this, need for a method of implementing sunlight with one LED by using a method of combining phosphors with one light source, rather than a method of combining red, blue, and yellow LEDs. Using this method, the characteristics of an artificial sunlight LED device with a spectrum similar to that of sunlight were demonstrated by implementing LED devices of various color temperatures with high color rendering by injecting phosphors into a 405nm deep blue LED light source. In order to find the spectrum closest to sunlight, different combinations of phosphors were repeatedly fabricated and tested. In addition, reliability and mass productivity were verified through temperature and humidity tests and ink penetration tests.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.415-422
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• 2013

This study was conducted to establish the optimum LED light source and quality for growth of Wasabia japonica seedlings in the LED chamber plant factory system. The light treatments were combined with four colors LED (red, blue, white, far-red), irradiation time ratio of the red and blue LED per minute(1:1, 2:1, 5:1, 10:1), and duty ratio of mixed light (100%, 99%, 97%). The growth response of W. japonica was the greatest in the R + B mixed light treatment, and seedlings grown in the red LED alone was higher than blue LED alone in the monochromic radiation treatments. In the R + B mixed LED, 1:1 ratio of R and B was the best for total biomass and tiller production. In mixed light treatments, the growth response of W. japonica was highest in the 100% duty ratio with R + B mixed light, while that was highest in the 97% duty ratio with R + B + W mixed light. Leaf area and dry weight were increased in the red light treatment alone, while specific leaf area was increased in the blue light alone. With the increasing red LED light ratio, leaf area and dry weight of W. japonica was significantly increased under the R + B mixed light treatment. In mixed light treatments, the leaf growth responses of W. japonica was highest in the 97% duty ratio with R+B mixed light, while that was highest in the 100% duty ratio with R + B + W mixed light. For cultivating W. japonica in a plant factory, treating red LED supplemented with a blue light or higher ratio of the red to blue LED was benefit to promote the growth of W. japonica.

• Journal of Information Display
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• v.11 no.3
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• pp.123-127
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• 2010

Fluorescent white organic light-emitting diodes showing high color-rendering indices (CRIs) of up to 81 was demonstrated, with a silicon-cored anthracene derivative (PATSPA) doped with DPAVBi utilized as the deep-blue host and dye materials, and the commercial dyes rubrene and DCM2 utilized as the orange- and red-light-emitting dyes. The devices, consisting of three emissive layers, showed bright-white-light emission, but the ratio of the blue peak to the orange and red peaks changed with the current density and the thickness of the blue emissive layer. A high CRI was achieved with the use of a deep-blue emitter doped in a novel host and by optimizing the blue-layer thickness. The device with a blue-layer thickness of 10 nm showed the Commission Internationale de l'Eclairage (CIE) color coordinate of (0.33, 0.35), a high CRI of 81, and a moderate external quantum efficiency of 2% at a current density of $2.5\;mA/cm^2$.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.1
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• pp.99-112
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• 2020

A edible mushroom, Hypsizygus marmoreus is commercially cultivated. However, the researches of cultivation and physiological characteristics were not conducted in Korea. In this study, we conducted on artificial cultivation of H. marmoreus and elucidated the effect of light on the mycelial growth and fruit body development using LED light sources with different wavelength; blue (peak wave length 460nm), green(peak wave length 530nm), yellow(peak wave length 590nm), red(peak wave length 630nm), and white as positive control. Mycelial growth of H. marmoreus strains were inhibited about 30~40% in inhibition ratio under the illumination with blue, green, yellow LED light. However, red LED light was not inhibited. Elongation of stipe was effective under the long wave length such as yellow and red light. Abnormal fruit body was produced under the long wavelength and dark. However, development of pileus was effective under the short wavelength such as green and blue light. Also, as a result of cultivation with mixed light for high quality and harvest, many effective numbers and yields of fruiting bodies were obtained in the mixed treatment of blue and white light, and pileus developed well.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.66-66
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• 2018

Light fermentation has been conducted under different light conditions such as normal dark light, white light, and light emitting diodes (LEDs) various color (blue, green, red, white on blueberry powder with fermenting bacteria Bacillus subtilis (B2). The bacteria B2 was isolated and identified by 16S rRNA sequencing method. RYRP biologically converted to secondary metabolites through light fermentation in the presence of Bacillus subtilis, the bacteria actively involved in bioconversion process. LEDs fermentation to enhance the production of phenolic content while comparing to normal dark and white light. Among the different color LEDs, blue LEDs mediated fermentation showed higher amount of total phenolic and flavonoid content. Then blue LEDs mediated fermented compound were characterized by FTIR and GC-MS, subsequently the compound was analyzed antioxidant activity tests and the antioxidant activity exhibited higher. This is the first study to demonstrate that B. subtilis-LEDs mediated fermentation is useful for facilitating phenolic compound production and enhancing antioxidant activity, which may have greater application fermentation fields.

• Journal of Environmental Science International
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• v.33 no.5
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• pp.305-322
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• 2024

The aim of this study was to investigate the effects of artificial light sources on the germination and seedling vigor of lettuce, aiming to identify the optimal artificial light source for producing high-quality seedlings. Lettuce cultivar of 'Tomalin' and 'Seonpunggold' exhibited the highest seed emergence in the Metal halide(MH) lamp and High-pressure sodium(HPS) lamp treatment group, while the emergence rate parameter, T50, was the fastest in the HPS lamp treatment group. Both cultivars showed good growth characteristics such as number of leaves, root length, and stem diameter under RGB-LED, and their seedling vigor was excellent as well. The plant height was smallest in the Red + Green + Blue LED treatment, but the leaves were round and thick, resulting in higher biomass and dry weight. Single light sources of Red LED and Blue LED led to reduced growth compared with that under the mixed light treatments. Chlorophyll content in lettuce varied with the type of artificial light, with both cultivars exhibiting the highest chlorophyll content in the Red + Green + Blue LED treatment. The most suitable artificial light for lettuce seedling growth was the Red + Green + Blue LED treatment.

• Environmental Analysis Health and Toxicology
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• v.10 no.3_4
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• pp.7-13
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• 1995

The most periodlengths($\tau$) of conidiation of Neurospora were shortened in the medium with Li$^+$, Rb$^+$, and Cs$^+$ under the blue or green light. The higher the concentration of the heavy metal elements are, the shorter the circadian lengths are. The largest differences between the maxima- and minima circadian lengths showed in the medium with LiCI, RbCl, and CsCI under the blue lights at 150 Lux, while the little differences of circadian lengths presented in 1 mM heavy metal elements at the 270Lux blue light. Li$^+$ under the blue light effected extremely much and in the order of Rb$^+$ and Cs$^+$ on the conidiation of Neurospora. Under the green lights at 270Lux, the smallest changes of the circadian lengths are resulted in the medium with heavy metal elements. The other way at the green light 150Lux, the conidiation is stimulated by lmM LiCI or RbCl for the average 0.71h and 0.29h longer than the periodlength of control 28.34h. The medium with Li$^+$ under the green light has less effect on the conidiation rhythm of Neurospora than with Rb$^+$ or Cs$^+$.