• Title/Summary/Keyword: Blood sedimentation

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Studies on the red blood cell sedimention rates in heteroplasma of chicken and goat (닭 및 산양의 이종plasma내에서 적혈구 침강에 대한 연구)

  • Yu, Chang-jun;Lee, Soo-doo
    • Korean Journal of Veterinary Research
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    • v.28 no.2
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    • pp.271-277
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    • 1988
  • In order to study the marked variation of red blood cell sedimentation rate in some species of animals, the packed cell volume, volume percentage of erythrocytes in whole blood, was reshuffled of 20%, 40% and 60% using heteroplasma of chicken and goat, and the red blood cell sedimentation rate was measured in Westergren tubes at $27{\pm}1^{\circ}C$ and $8{\pm}1^{\circ}C$. The results obtained were summarized as follows: 1. The values of packed cell volume(PCV) of goat and chicken were $40.7{\pm}4.1%$ and $30.2{\pm}2.2%$ respectively. 2. The sedimentation rates of reshuffled red blood cell were settled faster at lower PCV than higher PCV, i.${\acute{e}}$. there was a reverse relationship between the sedimention rate and PCV. 3. Red blood cells of chicken settled quickly, where as those of goat settled very slowly. Chicken red blood cell sedimented rapidly even in goat plasma, and goat red blood cell sedimented slowly in chicken plasma. These findings indicate that the plasma is not the only factor determining the rapid red blood cell sedimentation rate of chicken. 4. The sedimentation rate of reshuffled red blood cell of chicken and goat were accelerated at higher temperature than lower temperature.

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Erythrocyte Sedimentation Rates of Reshuffled Packed Cell Volume in Korean Native Goat (PVC수치와 온도가 한국재래 산양적혈구 침강에 미치는 영향)

  • Lee, Soo Doo;Kim, Young Hong;Yu, Chang Jun
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.55-59
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    • 1993
  • The packed cell volume(PCV) of Korean native goat, volume percentage of red blood cell in whole blood, was reshuffled of 20%, 40% and 60% using autoplasma, and erythrocyte sedimentation rate was measured in Westergren tubes at room temperature ($27{\pm}1^{\circ}C$) and low temperature ($8{\pm}1^{\circ}C$). The sedimentation rates of red blood cell obtained are summarized as follows. The erythrocyte sedimentation rates of Korean native goat are accelerated more at high temperature than low temperature. The erythrocyte sedimentation rates of reshuffled Korean native goat upon time are almost linear for several hours. The erythrocyte sedimentation rates of Korean native goat are settled faster at low PCV than higher PCV, i. e., there is a reverse relationshif between the erythrocyte sedimentation rate and packed cell volume.

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Effect of Dextrose, NaCl and Temperature on the RBC Sedimentation Rates in Goat (산양적혈구(山羊赤血球) 침강속도(沈降速度)에 미치는 온도(溫度), NaCl 및 Dextrose에 대하여)

  • Yu, Chang Jun
    • Current Research on Agriculture and Life Sciences
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    • v.2
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    • pp.109-114
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    • 1984
  • Eight Korean native black goats were used, $10m{\ell}$ of blood was collected from the Jugular vein into heparinized tubes a week interval. The heparinized blood was centrifuged for separation to blood plasma and corpuscles. The hematocrit, per centage of blood that is red blood cells, was reschuffuled of 10 %, 20 %, 30 %, 40 % and 50 % using blood plasma, 0.9 % NaCl solution and 5.4 % dextrose solution. The sedimentation rates of red blood cell obtained at $7^{\circ}C{\pm}1^{\circ}C$ and $27^{\circ}C{\pm}1^{\circ}C$ are summarized as follows. 1) The sedimentation rates of red blood cell were more increased by lower PCV%, i.e. there was a reverse relationship between the sedimentation rates and PCV% at any condition of these experiments. 2) The RBC were sedimented the most quickly in the NaCl solution and slower in the plasma compare with the dextrose solution at the same PCV%. 3) There was no temperature effect on the sedimentation rates between the two groups of $7^{\circ}C{\pm}1^{\circ}C$ and $27^{\circ}C{\pm}1^{\circ}C$(at PCV 20% and 10%), even though the temperature difference is $20^{\circ}C$.

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Red Cell Sedimentation Rates of Reshuffled Packed Cell Volume in Chicken (PCV수치를 변경시킨 닭적혈구 침강속도)

  • Yu, Chang Jun;Lee, Soo Doo
    • Current Research on Agriculture and Life Sciences
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    • v.8
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    • pp.115-118
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    • 1990
  • The packed cell volume(PCV) of chicken, volume percentage of erythrocytes in whole blood, was reshuffled of 20%, 40% and 60% using autoplasma, and the red blood cell sedimentation rate was measured in Westergren tubes at room temperature($27{\pm}1^{\circ}C$) and low temperature ($8{\pm}1^{\circ}C$). The results obtained were summarized as follows. 1. The sedimentation rates of chicken red blood cell were settled faster at low PCV than higher PCV, i,e., there was a reverse relationship between the sedimentation rate and PCV. 2. The sedimentation rate of chicken red blood cell was accelerated more at high temperature than low temperature. 3. The sedimentation rate of reshuffled chicken red blood cell by time was almost linear for several hours.

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Indirect calculation for volume of packed red cell(VPRC) by means of erythrocyte sedimentation rate(ESR) of diluted blood in dogs (개 회석혈의 적혈구침강율(ESR)에 의한 적혈구 침층용적(VPRC)치의 간접계산법)

  • Lee, Bang-whan;Park, Young-jun
    • Korean Journal of Veterinary Research
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    • v.29 no.2
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    • pp.41-49
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    • 1989
  • The study was undertaken to obtain indirect values of volume of packed red cell (VPRC) without centrifugation, using the erythrocyte sedimentation rate (ESR) of diluted blood in dogs. ESRs of diluted blood using the diluent of autologous plasma in which formed numerous RBC-rouleau clumps, autologous serum in which formed a few RBC-rouleau clumps, and 5% dextrose or 6% sucrose solutions in which formed numerous RBC-aggregation clumps, were accelerated. But, ESR of diluted blood using the 0.9% saline, D-S, ACD-B, CDP or D-PBS solutions were sluggish, because erythrocytes were dispersed in these diluents. Reliable values of VPRC on the basis of the correlating regressive equation to the ESR could be derived from values of$60^{\circ}$-angled-micro-ESR/40 min in the mixture, four parts of 5% dextrose solution and one part of whole blood. In the ESR values of diluted blood with low ratio, 1:1~3:1, $60^{\circ}$-micro-ESR was higher than $60^{\circ}$-Wintrobe-ESR. But, in the diluted blood with high ratio, 4:1~5:1, there was no different ESR values. For an aid of practical use, authers suggested a list of the $60^{\circ}$-micro-ESR/40 min in the diluted blood with equivalent VPRC of whole blood.

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Indirect calculation for volume of packed red cell(VPRC) by means of erythrocyte sedimentation rate (ESR) of blood diluted with isotonic dextrose solution in cattle (소에 있어서 등장 dextrose액으로 희석한 혈액의 적혈구 침강율(ESR) 측정에 의한 적혈구 침층용적(VPRC)치의 간접계산법)

  • Lee, Bang-whan;Bahk, Young-woo;Min, Byeong-man
    • Korean Journal of Veterinary Research
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    • v.30 no.1
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    • pp.107-112
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    • 1990
  • The purpose of this study is to derive indirect values of volume of packed red cell(VPRC), without centrifugation, from erythrocyte sedimentation rate(ESR) of blood diluted with isotonic dextrose solution in cattle. Each of ten blood samples taken from apprently healthy Korean cows and Holstein cows was used to produce eight different mixture of autologous plasma and blood corpuscles such that their values of VPRC lay between 15 to 50ml/100ml, and then each of the blood mixture was again diluted with 5% dextrose solution. The measurements of ESR using 45 degree-angled capillary hematocrit tube, 1.1~1.2mm bore, ($45^{\circ}$-micro-ESR) were practised for the diluted blood of various levels of VPRC under the ambient temperature of $10^{\circ}$, $20^{\circ}$ and $30^{\circ}C$. Reliable values of VPRC on the basis of the correlating linear regressive equation to the ESR could be derived from the values of $45^{\circ}$-micro-ESR/hr in the mixture of one part of whole blood and one part of 5% dextrose solution(1:1). For an aid of practical use, authors suggested a list of the $45^{\circ}$-micro-ESR/hr values of the diluted blood equivalent to VPRC of whole blood.

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The effect of Westergren tube angle, temperature and osmobility with the different percent of NaCl solution on erythrocyte sedimentation rate (적혈구 침강속도에 대한 Westergren tube 각도, 온도 및 NaCl 농도가 미치는 영향)

  • Kim, Do-kyun;Kim, Young-hong;Yu, Chang-jun
    • Korean Journal of Veterinary Research
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    • v.36 no.1
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    • pp.75-82
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    • 1996
  • Erythrocyte sedimentation rate is influenced by plasma protein, red cell itself and physiological conditions and it is clear that the measurement of erythrocyte sedimentation rate varies with technique and various environmental factors. The effect of temperature, angle of test tube, and osmobility with different percent of NaCl solution on erythrocyte sedimentation rate on sheep was determined by modified Westergren method. In sheep, as the angle of Westergren tube was decreased from $90^{\circ}$ to $45^{\circ}$, erythrocyte sedimentation rate was increased in both diluted plasma and NaCl solutions. As temperature was increased from $4^{\circ}C$ to $20^{\circ}C$ at $45^{\circ}$ angle. erythrocyte sedimentation rate was increased. In ruminants, this modified Westergren methods, erythrocyte 1 : plasma 9 instead of erythrocyte 4 : plasma 6(whole blood), enable them to have meanings as dog therefor, using this method, clinical can determine the erythrocyte sedimentation rate of ruminants for diagnosis. Because erythrocyte sedimentation rate was changed according to the angle of Westergren tube, temperature as erythrocyte diluted with NaCl, this study detected that the change of temperature, the angle of Westergren tube could change erythrocyte sedimentation rate by effecting red cell itself. The increase of osmobility owing to change of NaCl percent resulted in the decrease of rapid erythrocyte sedimentation rate. So this fact indicate that deformibility and the change of red cell volume have meaning in the change of erythrocyte sedimentation rate.

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Clinical Significance of Preoperative Inflammatory Parameters in Gastric Cancer Patients

  • Lee, Deuk Young;Hong, Seong Woo;Chang, Yeo Goo;Lee, Woo Yong;Lee, Byungmo
    • Journal of Gastric Cancer
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    • v.13 no.2
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    • pp.111-116
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    • 2013
  • Purpose: Chronic inflammation induces cancer and cancer induces local tissue damage with systemic inflammation. Therefore, the aim of this study is to investigate the potential relationship between the severity of inflammation and prognosis in cancer patients. Materials and Methods: This study enrolled 220 patients from January 2002 to December 2006 who underwent gastric surgery. We evaluated the relationship between preoperative inflammatory parameters (erythrocyte sedimentation rate, neutrophil-to-lymphocyte ratio) and other clinicopathological factors. Survival outcomes were compared according to the extent of inflammation. Results: Significant elevation of erythrocyte sedimentation rate was related with old age, increased neutrophil-to-lymphocyte ratio, decreased hemoglobin, increased carcinoembryonic antigen, increased tumor size and advanced TNM stage. Neutrophil-to-lymphocyte ratio was significantly correlated with old age, increased erythrocyte sedimentation rate and advanced TNM stage. In the univariate analysis, elevated erythrocyte sedimentation rate and increased neutrophil-to-lymphocyte ratio had significantly poorer survival than those without elevation (all P<0.05). However, the multivariate analysis failed to prove erythrocyte sedimentation rate and neutrophil-tolymphocyte ratio as independent prognostic factors. Conclusions: The elevation of erythrocyte sedimentation rate and neutrophil-to-lymphocyte ratio were correlated with poor prognosis in the univariate analysis and there was a strong correlation between inflammatory parameters (erythrocyte sedimentation rate and neutrophil- to-lymphocyte ratio) and tumor progression. Thus, erythrocyte sedimentation rate and neutrophil-to-lymphocyte ratio are considered useful as follow-up factors.

Relative Anticipated Erythrocyte Sedimentation Rate of Cattle Blood, as Measured by 45 Degree-Angled Capillary Hematocrit Tube, for Ambient Temperature and PCV Value (소 혈액의 45도 경사 모세관에 의한 ESR측정에 있어서 환경온도 및 PCV에 상관하는 관계적 예기ESR치)

  • Lee, Bang-whan
    • Korean Journal of Veterinary Research
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    • v.27 no.2
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    • pp.339-345
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    • 1987
  • Each of twenty blood samples taken from apparently healthy Korean Cows was used to produce five different mixtures of autologous plasma and blood corpuscles such that their values of packed cell volume (PCV) lay between 10 to 50ml/l00ml. The measurements of erythrocyte sedimentation rate (ESR) using 45 degree-angled capillary hematocrit tube, 1.1-1.2mm bore, ($45^{\circ}-micro-ESR$) were practised for the blood of various levels of PCV under the ambient temperature of ${10^{\circ}C}$, ${20^{\circ}C}$ and ${30^{\circ}C}$. Correlation of ESR to ambient temperature showed linear regression each in five levels of PCV. ESR increased with ascending ambient temperature, and magnitude of the increase of ESR became greater as the level of PCV lowered. Correlation of ESR, of which values were transformed by the linear regression equation correlated to ambient temperature to PCV showed curvilinear regression each in three levels of ambient temperature, and ESR was increased with decreasing PCV. The data were statistically analysed and a list of relative anticipate $45^{\circ}-micro-ESR$ values for PCV and ambient temperature was presented.

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Relative Anticipated Erythrocyte Sedimentation Rate of Cattle Blood, as Measured by 45 Degree-Angled Wintrobe Hematocrit Tube, for Ambient Temperature and PCV Value (소 혈액의 45도-경사-Wintrobe관에 의한 적혈구침강율 측정에 있어서 환경온도 및 적혈구침층용적치에 상관하는 관계적 예기 적혈구침강율)

  • Lee, Bang-whan;Park, Young-woo;Shin, Jong-uk
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.187-192
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    • 1988
  • Each of twenty blood samples taken from apparently healthy Korean cows was used to produce five different mixtures of autologous plasma and blood corpuscles such that their values of packed cell volume(PCV) lay between 10 to 50ml/100ml. The measurement of erythrocyte sedimentation rate (ESR) using 45 degree-angled Wintrobe hematocrit tube, 3mm bore, ($45^{\circ}$-Wintrobe-ESR) was practiced for the blood of various levels of PCV under the ambient temperature of $10^{\circ}C$, $20^{\circ}C$ and $30^{\circ}C$. Correlation of the ESR to the ambient temperature showed linear regression each in five levels of PCV. The ESR increased with ascending ambient temperature, and magnitude of the increase of the ESR became greater as the level of PCV lowered. Correlation of the ESR to PCV showed curvilinear regression each in three levels of the ambient temperature, and the ESR was increased with decreasing PCV. The data were statistically analysed and a list of relative anticipated $45^{\circ}$-Wintrobe-ESR values for PCV and ambient temperature was presented.

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