• BMB Reports
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• 제39권6호
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• pp.766-773
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• 2006

Fragile-X-syndrome (FXS) is the most common type of inherited cognitive impairment. The underlying molecular alteration consists of a CGG-repeat amplification within the FMR-1 gene. The phenotype is only apparent once a threshold in the number of repeats has been exceeded (full mutation). The aim of this study was to characterize the FMR-1 CGG-repeat status in Argentine patients exhibiting mental retardation. A total of 330 blood samples from patients were analyzed by PCR and Southern blot analysis. Initially, DNA from 78 affected individuals were studied by PCR. Since this method is unable to detect high molecular weight alleles, however, we undertook a second approach using the Southern blotting technique to analyze the CGG repeat number and methylation status. Southern blot analysis showed an altered pattern in 14 out of 240 (6%) unrelated patients, with half of them presenting a mosaic pattern. Eight out of 17 families (47%) showed a (suggest deleting highlight). The characteristic FXS pattern was identified in 8/17 families (47%), and in 4 of these families 25% of the individuals presented with a mosaic model. The expansion from pre-mutation to full mutation was shown to occur both at the pre and post zygotic levels. The detection of FXS mutations has allowed us to offer more informed genetic counseling, prenatal diagnosis and reliable patient follow-up.

• 대한물리치료과학회지
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• 제11권1호
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• pp.5-13
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• 2004

The purpose of this study was to the Pre-Post maximal exercise for analyze the Physiological Variable(Lactate, LDH, Glucose) in the runners. The subjects were 12 volunteers 6 sprinters and 6 distance runners who agreed to intention of this study. Subjects performed until possible all-out trials. Blood samples were taken from a median cubital vein Pre-Post exercise and every rest 5min during the all-out trial. The results obtained were summarized as follow ; 1) Lactate of Pre-Post exercise in treadmill test showed no significant difference between sprinters and distance runners, but showed significant in sprinters and distance respectively 2) LDH of Pre-Post exercise in treadmill test showed no significant difference between sprinters and distance runners, and that showed no significant in sprinters and distance respectively 3) Glucose of Pre-Post exercise in treadmill test showed no significant difference between sprinters and distance runners, and showed no significant in distance, but showed significant in sprinters therefor, since the runners has an intermittent characteristic which includes a frequent momentary change, it needs the fitness training that being consisted of various training, in the training method, it needs the training process which is from the whole fitness to specific fitness to improve general physical ability.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1989년도 춘계학술대회
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• pp.81-83
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• 1989

In surgery the hemostatic control is very important for the parenchymatous organs. These organs consist of the fragile and the blood content tissues such as liver, spleen and kidney, etc.. One of the control methods to solve this problem is to insert the mono polar typed needle electrode, which gathers the thermal effect of microwave, directly into tissues so as to coagulate and stop the hemorrhage. This method has same advantages: First, the range of the heat energy is limited. Second, the coagulation, the hemostatic characteristic, and stability are excellent. Third, more convenient operation is possible. This paper is aimed to manufacture the microwave tissue coagulation system and to suggest the new direction for development, hereafter.

• Biomolecules & Therapeutics
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• 제16권1호
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• pp.40-45
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• 2008

The objective of this study was to investigate the single dose and 2-week repeated dose toxicity of Aceporol 330 in ICR mice following single intravenous administration and to compare its toxicity with a commercially available solubilizer of paclitaxel, Cremophor EL. In single dose toxicity test, $LD_{50}$ of Aceporol 330 in mice was estimated to be greater than maximum applicable dose, 4 ml/kg. However, $LD_{50}$ of Cremophor EL in male mice was determined to be 4 ml/kg. Maximum tolerated dose (MTD) of males and females in Aceporol 330-treated group and MTD of females in Cremophor EL-treated group were 3 ml/kg. MTD of males in Cremophor EL-treated group was less than 3 ml/kg. Characteristic toxic symptoms, and hematological and blood chemical changes were not observed after single dose and repeated dose of Aceporol 330 or Cremophor EL. No histopathological abnormalities were found in organs of all animal groups. Based on the linear pharmacokinetic property of paclitaxel and the higher $LD_{50}$ in mice, Aceporol 330 has a potential for use as a safer solubilizer for paclitaxel than Cremophor EL.

• 한국동물위생학회지
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• 제32권2호
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• pp.139-146
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• 2009

Bovine brucellosis is a zoonosis, long incubation period and chronic infectious disease, usually caused by Brucella abortus. This study was carried out to investigate the biotyping and biochemical characterization of B. abortus isolated from 208 farm 871 korean cattle and holstein diagnosed brucellosis by serological positive in Gyeongbuk province during the period from 2002 to 2006. B. abortus was isolated from 124 (14.2%) of 871 cattle, and isolated 110 (13.4%) of 820 Korean cattle and 14 (27.5%) of 51 holstein in breed. The uterus of korean cattle was isolated in 8 (17.8%) of 45 cattle and supramammary lymph none of holstein was isolated 11 (68.8%) of 16 cattle. 101 (12.5%) of 810 serological positive blood samples were isolated B. abortus. The isolation rate of B. abortus was correlated with antibody titers. The biochemical characterization of isolates was non-hemolytic, production of H$_2$S, oxidase-positive, catalase-positive, hydrolyzation of urea and growth of basic fuchsin dye medium. As a result, all of isolates was identified B. abortus bv 1. 124 isolates were susceptible to ampicillin, lincospectin, amikacin, gentamicin, kanamycin, neomycin, streptomycin, tetracycline, ciprofloxacin, norfloxacin and enrofloxacin.

• 대한수의학회지
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• 제22권2호
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• pp.171-174
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• 1982

The disease syndrome characterized by the acute vomiting and diarrhea with high mortality had been greatly epidemic in Korea since June, 1931 and it was followed serologically and electron microscopically for the clarification of the agent. The agent present in feces of dogs associated with this syndrome had characteristic feature in agglutinating pig red blood cells that was specifically inhibited by anti-CPV reference dog serum. This also showed the serological identity with the reference CPV antigen in immuno-diffusion. Electron micrograph of the material revealed parvovirus particles with size of 20nm and icosahedral structure. These results clearly indicated that CPV was the primary cause of canine epidemic prevailing in 1981 in Korea.

• 한국자기학회지
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• 제27권4호
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• pp.145-152
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• 2017

동맥스핀표지 기법(ASL)은 체내의 혈액을 이용하여 조직의 관류상태를 평가할 수 있는 자기공명영상 방법이다. 조영제를 사용하지 않는 비침습적 검사 특성과 정량적인 관류량의 측정이 가능하여 임상이나 연구목적으로 이용 빈도가 증가하고 있다. 아직까지는 ASL 방법이 조영제를 이용한 관류영상 방법에 비해 낮은 SNR과 영상화 과정에서의 여러 가지 변수의 최적화 과정이 어렵기 때문에 이로 인한 측정오차가 발생할 수 있다. 이를 개선하기 위해 다양한 기술을 적용한 ASL 방법들이 소개되고 있다. 본 논문은 ASL의 개요와 영상화 과정에서의 특징 및 다양한 기술, 임상적 적용에 대해 간단히 소개한다.

• 대한한의학원전학회지
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• 제28권2호
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• pp.61-66
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• 2015

Objectives : Through the analyze of formative factor of time sense and the vital movement temporal rhythm in Huang Di Nei Jing(黃帝內經). Methods : We find out the principle and methods of Chronomedicine and Health Preserving in Huang Di Nei Jing(黃帝內經), and this finding will direct the life nurturing for later generations. We find that time sense had been established in Huang Di Nei Jing(黃帝內經), which had yin-yang and five elements characteristic. Results : There have temporal rhythm of functions of zang-fu viscera, meridian and collateral, qi and blood. Based on the theory of correspondence between human body and natural environment, established principle of health maintenance about based on yin and yang, temporal rhythm of visceral qi. Conclusions : Established health maintenance methods based on time such as adjusting-spirit according to time, regular daily life, dietary regimen according to time, needling and moxibustion according to time. These methods will guide syndrome differentiation and therapeutic method and Medication which based on suitable time.

• 한국임상수의학회지
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• 제19권4호
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• pp.450-454
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• 2002

A five-year-old, female Great Dane dog with edema, localized trauma, mild pain, and lameness of the right hind limb was referred to the Veterinary Medical Teaching Hospital of Chungbuk National University. This dog had a history of mammary tumor excisions 6 months ago. Abnormal changes were not seen in the values of complete blood count and serum biochemical tests. But pedal direct lymphangiography using aqueous-based radiographic agent showed the obstructed lymph flow in right popliteal lymph node. Based on these observations, the dog was suspected as lymphedema resulted from lymph drainage flilure without any other possibilities of inflammation or other causes. Although recommended chemotherapy and physiotherapy had been applied for resolvinr presented problems for one month, there was no improvement on edema of damaged region and any other clinical signs. Therefore, the necropsy was performed after euthanasia under agreement of the owner of patient. In histopatholofical examination, the most characteristic lesions in the mass of femoral region were diffuse edema fibrosis and neoplastic cells in the lymphatics. Also, the neoplastic cells were very similar to those found in the tumor mass of mammary gland, which had diagnosed as fibrosing carcinoma. These facts suggested that the cause of obstructed lymph flow was the neoplasia in lymphatics of the right hind limb. With these results, a diagnosis of malignant lymphedema was made in this dog.

• Journal of Acupuncture Research
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• 제17권3호
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• pp.75-86
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• 2000

Objectives : This study is to applied effectively to the acupuncture treatment for disorders following whiplash injuries. Methods : This study suggests five various ways and principtes of treating disorders following whiplash injuries by means of acupuncture. First principle is that acupuncture treatment is carried out on acupoint far from cervical spine at acute stage, on the other hand, Extraordinary Vessel and other acupuncture treatment such as five element acupuncture treatment is applied at chronic stage in order to control balance disoders due to cervical pathology. Second is acupuncture treatment will be performed in group of three part of Meridian System-Yangmyung, Taeyang, Soyang-according to cervical kinetic, its characteristic and location of pain. Third is acupuncture treatment could be performed on basis of theory of Eohyel-disorders and disturbance of blood circulation. Fourth is to decrease tension of SCM muscle, because disorders following whiplash injuries has much association to injury of CM muscle and is simillar to tension syndroms of SCM muscle. fifth is taping therapy could control and decrease disorders following whiplash injuries based on spiral balance taping theory. Conclusions : These results suggest that acupuncture treatment for disorders following whiplash injuries will significally decrease pain and control syndrom caused by cervical disorder.