• Title/Summary/Keyword: Block Assembly

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Ordered Fragmentation of pDNA induced by PEG-PLL block copolymer -Correlation between Condensation degree and Biological Activity by Cell-Free System-

  • Osada, Kensuke;Doi, Motoyoshi;Shiotani, Tomonori;Yamasaki, Yuichi;Kataoka, Kazunori
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.254-254
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    • 2006
  • The sensitivity of plasmid DNA (pDNA) to S1 nuclease, an enzyme to cleave a single-strand DNA, was dramatically modulated through a supramolecular assembly (polyion complex micelle) with a synthetic block copolymer, poly(ethylene glycol)-b-poly(L-lysine) (PEG-PLL). The pDNA condensed in stoichiometric charge ratio was cleaved into 7 fragments each being 10/12, 9/12, 8/12, 6/12, 4/12, 3/12, and 2/12 of the original DNA length, on the other hand, the pDNA condensed in higher charge ratios (>4), were digested into non-specific manner. Condensation of the pDNA was investigated from two viewpoints that how does the rigid DNA molecules fold and condense and how does the condensation influence their biological activity.

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A study for production simulation model generation system based on data model at a shipyard

  • Back, Myung-Gi;Lee, Dong-Kun;Shin, Jong-Gye;Woo, Jong-Hoon
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.8 no.5
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    • pp.496-510
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    • 2016
  • Simulation technology is a type of shipbuilding product lifecycle management solution used to support production planning or decision-making. Normally, most shipbuilding processes are consisted of job shop production, and the modeling and simulation require professional skills and experience on shipbuilding. For these reasons, many shipbuilding companies have difficulties adapting simulation systems, regardless of the necessity for the technology. In this paper, the data model for shipyard production simulation model generation was defined by analyzing the iterative simulation modeling procedure. The shipyard production simulation data model defined in this study contains the information necessary for the conventional simulation modeling procedure and can serve as a basis for simulation model generation. The efficacy of the developed system was validated by applying it to the simulation model generation of the panel block production line. By implementing the initial simulation model generation process, which was performed in the past with a simulation modeler, the proposed system substantially reduced the modeling time. In addition, by reducing the difficulties posed by different modeler-dependent generation methods, the proposed system makes the standardization of the simulation model quality possible.

Preparation and characterization of fluorinated poly(arylene ether sulfone) block copolymers for fuel cell applications (고분자 연료전지용 불소계 poly(arylene ether sulfone) 블록 공중합체 전해질막의 합성 및 특성연구)

  • Yoo, Min-Chul;Chang, Bong-Jun;Kim, Jeong-Hoon;Lee, Soo-Bok;Lee, Yong-Taek
    • New & Renewable Energy
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    • v.2 no.4 s.8
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    • pp.46-55
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    • 2006
  • 본 연구는 불소그룹을 함유한 술폰화된 아릴렌에테르계 블록 공중합체 고분자 전해질막의 제조 및 연료전지 특성에 관한 것이다. 이러한 불소그룹을 함유한 술폰화된 아랄렌에테르계 블록 공중합체를 제조하기 위하여 양말단에 불소계 비닐기를 가지면서, 고분자 전환시 상온에서 술폰화 가능한 biphenyl계 단량체와 술폰화가 불가능한 sulfonyl계 단량체를 각각 합성하였다. Biphenyl계 단량체로 부터 올리고머를 합성한 후 sulfonyl계 단량체와 열적 고리화 부가중합을 하여 다양한 몰조성을 갖는 일련의 perfluorocyclobutane(PFCB)기를 포함하는 블록 공중합체를 제조하였다. 제조된 블록 공중합체를 상온에서 술폰화제인 chlorosulfonic acid(CSA)를 이용하여 후술폰화시켜 강산 이온기인 sulfonic acid를 biphenyl계 올리고머 부분에 선택적으로 도입하였다. 이렇게 제조된 술폰화된 고분자를 제막한 후 연료전지 특성을 Nafion-115와 비교하였다. 술폰화가 되는 올리고머 블록의 비율 증가에 따라 이온교환능력 (IEC)이 증가하였고 , 그에 따른 팽윤도 역시 증가하는 것을 보였다. 술폰화된 고분자들은 건조 및 습윤 상태에서도 기계적 강도가 우수하였다. 최적화된 술폰화 블록 고분자(S-2) 를 대상으로 membrane electrolyte assembly(MEA) 를 제조하여 연료전지 초기성능을 측정한 결과 Nafion-112와 유사한 전기화학적 성능을 나타내었다.

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Stability Studies of Biodegradable Polymersomes Prepared by Emulsion Solvent Evaporation Method

  • Lee Yu-Han;Chang Jae-Byum;Kim Hong-Kee;Park Tae-Gwan
    • Macromolecular Research
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    • v.14 no.3
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    • pp.359-364
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    • 2006
  • Di-block copolymers composed of two biocompatible polymers, poly(ethylene glycol) and poly(D,L-lactide), were synthesized by ring-opening polymerization for preparing polymer vesicles (polymersomes). Emulsion solvent evaporation method was used to fabricate the polymersomes. Scanning electron microscope (SEM) images confirmed that polymersomes have a hollow structure inside. Confocal laser microscope and optical microscope were also used to verify the hollow structure of polymersomes. Polymersomes having various sizes from several hundred nanometers to a few micrometers were fabricated. The size of the polymersomes could be readily controlled by altering the relative hydrodynamic volume fraction ratio between hydrophilic and hydrophobic blocks in the copolymer structure, and by varying the fabrication methods. They showed greatly enhanced stability with increased molecular weight of PEG. They maintained their physical and chemical structural integrities after repeated cycles of centrifugation/re-dispersion, and even after treatment with surfactants.

Finite Element Analysis of Engine Cylinder Block and Main Bore for Reliable Design (신뢰성 설계를 위한 엔진 실린더 블록과 메인 보어의 유한요소해석)

  • Yang Chulho;Han Moonsik
    • Transactions of the Korean Society of Automotive Engineers
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    • v.13 no.4
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    • pp.39-48
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    • 2005
  • Finite element analyses have been performed for the purpose of obtaining the robust and reliable design of engine cylinder block. Fatigue under high cycle operating loads is a primary concern and is evaluated by a probabilistic method. The robust and reliable design by a probabilistic method can provide satisfactory design conditions for the performance of the system under the influence of noise factors. Therefore, the design by this method will be desensitized to the uncontrollable noise factors. The simple methodology evaluates the distortion of main bore is proposed for the purpose of maintaining a well-controlled clearance between the crankshaft and main bores. The proposed methodology has proven a capability of predicting the distortion of the main bore under assembly, thermal, and firing loads. The calculated results are correlated well with the experimental ones.

Effects on TMR and Airflow in HDD Cavity due to Disk Damper Shapes (디스크 댐퍼 현상이 HDD 내부 유동 및 TMR에 미치는 영향)

  • Um, Yo-Han;Rhim, Yoon-Chul
    • Transactions of the Society of Information Storage Systems
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    • v.2 no.1
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    • pp.32-37
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    • 2006
  • The recording density of a hard disk drive(HDD) has been increased so rapidly that the storage capacity of a commercial HDD for the personal computer already reaches several hundred giga-bytes recently. Many technologies related to the HDD, such as servo, media, actuator dynamics, thermo and fluid dynamics, etc. must be developed together to realize higher recording density. Especially, airflow inside the HDD cavity has been concerned as the rotational speed of the disk increases. Typical problem due to the airflow is the off-track vibration of a head stack assembly(HSA) as the airflow collides with the E-block, suspensions, and sliders, i.e., the flow induced vibration(FIV). This problem is one of the most significant sources of the track mis-registration(TMR) so that it must be resolved. In this study, disk damper shape is modified to minimize the influence of airflow on the HSA. Modified disk dampers, which change the flow field of the inside cavity of a HDD, show good effects not only on the disk vibration but also on the off-track vibration of a HSA. Vibrations of E-block and slider have been measured with LDV and the airflow field inside the HDD cavity has been analyzed with commercial CFD program to verify these effects.

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Parallel Implementation of SPECK, SIMON and SIMECK by Using NVIDIA CUDA PTX (NVIDIA CUDA PTX를 활용한 SPECK, SIMON, SIMECK 병렬 구현)

  • Jang, Kyung-bae;Kim, Hyun-jun;Lim, Se-jin;Seo, Hwa-jeong
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.31 no.3
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    • pp.423-431
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    • 2021
  • SPECK and SIMON are lightweight block ciphers developed by NSA(National Security Agency), and SIMECK is a new lightweight block cipher that combines the advantages of SPECK and SIMON. In this paper, a large-capacity encryption using SPECK, SIMON, and SIMECK is implemented using a GPU with efficient parallel processing. CUDA library provided by NVIDIA was used, and performance was maximized by using CUDA assembly language PTX to eliminate unnecessary operations. When comparing the results of the simple CPU implementation and the implementation using the GPU, it was possible to perform large-scale encryption at a faster speed. In addition, when comparing the implementation using the C language and the implementation using the PTX when implementing the GPU, it was confirmed that the performance increased further when using the PTX.

Nano-Scale Patterning by Gold Self-Assembly on PS-PB-PS Triblock Copolymer Thin Film Templates (PS-PB-PS 삼블럭 공중합체 박막형판에서의 금의 자기응집에 의한 Nano-Scale 패턴형성)

  • Kim, G.;Libera, M.
    • Elastomers and Composites
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    • v.34 no.1
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    • pp.45-52
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    • 1999
  • This paper describes how the gold particles self assemble on the specific phase on the microphase separated block copolymer thin film and form a well ordered patterns. For this study, polystyrene-polybutadiene-polystyrene (PS-PB-PS) triblock copolymer (30wt % PS) thin films (${\sim}100nm$) having a cylindrical morphology were cast from 0.1wt% toluene solution to be used as polymer thin film templates. The films having either vertical PS cylinders or in-plane PS cylinders in PB matrix from each different solvent evaporation condition were obtained. Cross-sectional transmission electron microscopy(TEM) was used to study the surface and bulk morphologies of block copolymer thin films. Small amount of gold particles was evaporated on a block copolymer thin film template to obtain a nano-scale pattern. When an as-cast thin film template was used, gold particles preferentially self assemble on the low surface tension PB phase and a relatively well ordered pattern in nano-scale was produced. However, after the formation of a low surface energy PB rich layer upon annealing, a gold self-assembled pattern was not observed.

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Design of Integrated Process-Based Model for Large Assembly Blocks Considering Resource Constraints in Shipbuilding (자원제약을 고려한 조선 대조립 공정의 통합 프로세스 기반 모델 설계)

  • Jeong, Eunsun;Jeong, Dongsu;Seo, Yoonho
    • Journal of the Korea Society for Simulation
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    • v.28 no.2
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    • pp.107-117
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    • 2019
  • Because shipbuilding is single-product production with limited resources, production management technology is essential to manage the resources effectively and maximize the productivity of ship-process. Therefore, many shipbuilding companies are conducting research on ship production plan and process considering various constraints in the field by applying modeling and simulation. However, it is difficult to provide accurate production plan on sudden schedule and process changes, and to understand the interconnectivity between the processes that produce blocks in existing research. In addition, there are many differences between the production planning and field planning because detailed processes and quantity of blocks can not be considered. In this research, we propose the integrated process-based modeling method considering process-operation sequences, BOM(Bill of materials) and resource constraints of all the scheduled blocks in the indoor system. Through the integrated process-based model, it is easy for the user to grasp the assembly relationship, workspace and preliminary relationship of assembly process between the blocks in indoor system. Also, it is possible to obtain the overall production plan that maximizes resource efficiency without the separate simulation and resource modeling procedures because resource balancing that considers the amount of resource quantity shared in the indoor system is carried out.

Development of Movable nose crossing turnout on concrete track using Fast Clip (Fast clip을 적용한 콘크리트궤도용 노스가동 분기기 개발)

  • Hwang, Kwang-Ha;Ryou, Ki-Tae;Park, Chun-Bok;Park, Kwang-Ryoun;Yun, Byung-Hyun
    • Proceedings of the KSR Conference
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    • 2011.10a
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    • pp.287-296
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    • 2011
  • Turnout is a mechanical installation enabling railway trains to be guided from one track to another at a railway junction. A movable nose crossing frog is a device used at a railway turnout to eliminate the gap at the common crossing (High manganese, block, assembly crossing)which can cause impact damage, noise and vibration. Our government has a plan speed up of conventional line to 250km/h semi-high speed. We had already developed flexible turnout with fixed crossing(High manganese) and SFC fastening system can cover in the semi-high speed line In this study is about development of the movable nose crossing turnout available Semi-high speed line on concrete track. This paper describes about geometry, attack angle, bending stress at the nose, switching force, safety of continuous welded long rails. This movable nose crossing turnout is expected greatly increases passing speed of turnout in national railway.

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