Proceedings of the Polymer Society of Korea Conference (한국고분자학회:학술대회논문집)

The Polymer Society of Korea (한국고분자학회)
권호 표지

Ordered Fragmentation of pDNA induced by PEG-PLL block copolymer -Correlation between Condensation degree and Biological Activity by Cell-Free System-

  • Osada, Kensuke (Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo) ;
  • Doi, Motoyoshi (Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo) ;
  • Shiotani, Tomonori (Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo) ;
  • Yamasaki, Yuichi (Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo) ;
  • Kataoka, Kazunori (Division of Clinical Biotechnology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo)
  • Published : 2006.10.13
Download PDF
⟨ Previous Next ⟩

Abstract

The sensitivity of plasmid DNA (pDNA) to S1 nuclease, an enzyme to cleave a single-strand DNA, was dramatically modulated through a supramolecular assembly (polyion complex micelle) with a synthetic block copolymer, poly(ethylene glycol)-b-poly(L-lysine) (PEG-PLL). The pDNA condensed in stoichiometric charge ratio was cleaved into 7 fragments each being 10/12, 9/12, 8/12, 6/12, 4/12, 3/12, and 2/12 of the original DNA length, on the other hand, the pDNA condensed in higher charge ratios (>4), were digested into non-specific manner. Condensation of the pDNA was investigated from two viewpoints that how does the rigid DNA molecules fold and condense and how does the condensation influence their biological activity.

Keywords