Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
권호 표지

Stability Studies of Biodegradable Polymersomes Prepared by Emulsion Solvent Evaporation Method

  • Lee Yu-Han (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Chang Jae-Byum (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Kim Hong-Kee (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Park Tae-Gwan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Di-block copolymers composed of two biocompatible polymers, poly(ethylene glycol) and poly(D,L-lactide), were synthesized by ring-opening polymerization for preparing polymer vesicles (polymersomes). Emulsion solvent evaporation method was used to fabricate the polymersomes. Scanning electron microscope (SEM) images confirmed that polymersomes have a hollow structure inside. Confocal laser microscope and optical microscope were also used to verify the hollow structure of polymersomes. Polymersomes having various sizes from several hundred nanometers to a few micrometers were fabricated. The size of the polymersomes could be readily controlled by altering the relative hydrodynamic volume fraction ratio between hydrophilic and hydrophobic blocks in the copolymer structure, and by varying the fabrication methods. They showed greatly enhanced stability with increased molecular weight of PEG. They maintained their physical and chemical structural integrities after repeated cycles of centrifugation/re-dispersion, and even after treatment with surfactants.

Keywords

References

  1. M. Antonietti and S. Förster, Adv. Mater., 15, 1323 (2003) https://doi.org/10.1002/adma.200300010
  2. B. M. Discher, Y. Won, D. S. Ege, J. C. Lee, F. S. Bates, D. E. Discher, and D. A. Hammer, Science, 284, 1143 (1999) https://doi.org/10.1126/science.284.5417.1143
  3. H. E. Warriner, S. H. J. Idziak, N. L. Slack, P. Davidson, and C. R. Safinya, Science, 271, 969 (1996) https://doi.org/10.1126/science.271.5251.969
  4. K. Yu and A. Eisenberg, Macromolecules, 31, 3509 (1998) https://doi.org/10.1021/ma971419l
  5. Y. S. Nam, H. S. Kang, J. Y. Park, T. G. Park, S. Han, and I. Chang, Biomaterials, 24, 2053 (2003) https://doi.org/10.1016/S0142-9612(02)00641-5
  6. M. Yokoyama, G. S. Kwon, T. Okano, Y. Sakurai, T. Seto, and K. Kataoka, Bioconjugate Chem., 3, 295 (1992) https://doi.org/10.1021/bc00016a007
  7. M. Yokoyama, T. Okano, Y. Sakurai, and K. Kataoka, J. Control. Release, 32, 269 (1994) https://doi.org/10.1016/0168-3659(94)90237-2
  8. R. Gref, A. Domb, P. Quellac, T. Blunk, R. H. Muller, J. M. Verbavatz, and R. Langer, Adv. Drug Deliver. Rev., 16, 215 (1995) https://doi.org/10.1016/0169-409X(95)00026-4
  9. M. Yokoyama, T. Okano, Y. Sakurai, S. Suwa, and K. Kataoka, J. Control. Release, 39, 351 (1996) https://doi.org/10.1016/0168-3659(95)00165-4
  10. G. Kwon, M. Naito, M. Yokoyama, T. Okano, Y. Sakurai, and K. Kataoka, J. Control. Release, 48, 195 (1997) https://doi.org/10.1016/S0168-3659(97)00039-4
  11. H. K. Kim and T. G. Park, J. Control. Release, 98, 115 (2004) https://doi.org/10.1016/j.jconrel.2004.04.020
  12. Y. Yamamoto, K. Yasugi, A. Harada, Y. Nagasaki, and K. Kataoka, J. Control. Release, 82, 359 (2002) https://doi.org/10.1016/S0168-3659(02)00147-5
  13. E. Jule, Y. Yamamoto, M. Thouvenin, Y. Nagasaki, and K. Kataoka, J. Control. Release, 97, 407 (2004)
  14. B. M. Discher, H. Bermudez, D. A. Hammer, and D. E. Discher, J. Phys. Chem. B, 106, 2848 (2002) https://doi.org/10.1021/jp011958z
  15. D. E. Discher and A. Eisenberg, Science, 297, 967 (2002) https://doi.org/10.1126/science.1074972
  16. F. Meng, C. Hiemstra, G. H. M. Engbers, and J. Feijen, Macromolecules, 36, 3004 (2003) https://doi.org/10.1021/ma034040+
  17. F. Meng, G. H. M. Engbers, and J. Feijen, J. Control. Release, 101, 187 (2005) https://doi.org/10.1016/j.jconrel.2004.09.026
  18. H. S. Yoo and T. G. Park, J. Control. Release, 70, 63 (2001) https://doi.org/10.1016/S0168-3659(00)00340-0
  19. F. Szoka and D. Papahadjopoulos, Proc. Natl. Acad. Sci. U. S. A., 75, 4194 (1978)
  20. F. Ahmed and D. Discher, J. Control. Release, 96, 37 (2004) https://doi.org/10.1016/j.jconrel.2003.12.021
  21. G. Gregoriadis, Liposome Technology, CRC Press, Daejeon, 1993
  22. J. H. Jeong, D. W. Lim, D. K. Han, and T. G. Park, Colloid Surface B., 18, 371 (2000) https://doi.org/10.1016/S0927-7765(99)00162-9
  23. Y. Tsutsumi, M. Onda, S. Nagata, B. Lee, R. J. Kreitman, and I. Pastan, Proc. Natl. Acad. Sci. U. S. A., 97, 8548 (2000)