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Effect of Serum and Serum Free Media on the Developmental Competence of OPU Derived Bovine IVP Embryo

  • Choi, Byung-Hyun;Park, Bun-Young;Kong, Rami;Son, Mi-Ju;Park, Chan-Sang;Shin, Nyeon-Hak;Cheon, Hye-Young;Yang, Yeong-Rok;Lee, Jin-Woo;Jin, Jong-In;Kong, Il-Keun
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.4
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    • pp.305-310
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    • 2019
  • Embryos produced with serum show the alterations in their ultrastructure, impaired compaction, abnormal blastulation, aberrant mRNA expression profiles and large calf syndrome with greater incidences of stillbirths and deaths after birth. The aim of the present study was to describe in vitro embryo production by analyzing embryo production, fetal production and pregnancy rate in free-serum medium. The OPU-IVP data used in this study from 2016. Approximately, sixteen cows (Hanwoo), which belonged to the Institute of Gyeongsang National University, were used. Two experimental group is used in this study. Serum groups were conducted in March to July and free-serum group was conducted in September to December. The recovered cumulus-oocyte complexes were morphologically classified to four grades based on the compaction of cumulus cells layers and homogeneity of the cytoplasm. The number of oocyte was significantly greater in serum groups than that in free-serum groups (29.61 ± 0.63 vs. 15.6 ± 0.62; p < 0.05). Between serum and free-serum groups indicate that average of 1st and 2nd grade oocytes were no difference (2.38 ± 1.67 vs. 2.38 ± 1.48; p > 0.05), but number of 3rd and 4th grade oocytes were greater in serum groups than that in free-serum groups (7.31 ± 7.64 vs. 5.60 ± 6.29; p < 0.05). Embryo cleaved competence was higher in rate in free-serum groups than that in serum groups (62.1% vs. 58.3; p < 0.05). However, blastocyst developmental rate was no difference between serum and free-serum groups (33.1% vs. 43.5%; p < 0.05). 986 recipients were used for embryo transfer. Pregnancy rate was indicated that between serum and free-serum group was no difference (54.6% vs. 56.3%; p < 0.05). In conclusion, we developed the free-serum system for production of in vitro bovine embryos in order to meet the developmental and qualitative requirements for large scale commercial use.

A Review on the Change of Health Policy Based on Ethical Issues (윤리적 쟁점을 중심으로 한 보건의료정책 변화의 고찰)

  • Lee, Dong Hyun;Kim, So Yoon;Sohn, Myongsei
    • Health Policy and Management
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    • v.28 no.3
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    • pp.222-225
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    • 2018
  • Health policy is a historical product in the process of development, including the political and economic factors of the state as well as the social and cultural elements of the country. Bioethics began to debate the ethical questions that arise in the overall process of life's birth and death, and gradually evolved by presenting ethical directions for various social phenomena. Especially, according to the moral awakening of 'scientific medicine' which caused in some human problems in the rapidized scientific society from the late 19th century to the early 20th century, as a result of distress including the concept of various social relations, it is possible to say that it has reached the bioethics. Although health policy and bioethics are different in terms of starting and concept, they can be found in common with social, cultural, and political diversity in the times. In 2004, 'Bioethics Law' was enacted through the issue of research ethics in the life sciences. Therefore, in order to examine ethical aspects of current health policy direction and major issues, it can be divided into before and after enactment of 'Bioethics Law' in 2004. The authors would like to examine how the evolution of the ethical viewpoint on the health policy has changed in line with the enactment of the 'Bioethics Law' and how it is trying to solve it from an ethical point of view. Through the various events that took place in the 1990s and the 2000s, various discussions on bioethics were conducted in Korea. Prior to the enactment of the 'Bioethics Law,' ethical judgments of professions, distribution of healthcare resources, if the discussion focused on the ethical judgment of abortion, and the various events that appeared in the early 2000s became the beginning to inform that the ethical debate about the life, death, and dignity of human beings began in earnest in Korea with the enactment of the 'Bioethics Law.' Since then, 'Hospice and Palliative care Law' which was enacted in 2017, is based on the fact that the health policy of our country focuses on the treatment of the past diseases, health promotion, and delivery of health care services. It was an opportunity to let them know that even the quality problems were included. Therefore, considering the various circumstances, the ethical issue facing Korea's health care system in the future is the change of the demographic structure due to aging and what is to be considered as the beginning and the process of life in the overall process of life. It is the worry about how to die and when it sees as death. This has far exceeded the paradigm of traditional health care policies such as disease prevention and management and health promotion, and calls for innovative policy response at the national level that reflects the new paradigm, which in many cases creates a predictable ethical environment. And health policy should be shifted in the direction of future ethical review considering sustainability in the development process of future health care rather than coercive management.

Concept, Pioneers, and Characteristics of Bibliodrama (비블리오드라마의 개념, 선구자들, 그리고 근본성격)

  • Koh, Won Seok
    • Journal of Christian Education in Korea
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    • v.62
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    • pp.101-133
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    • 2020
  • This study is an attempt to grasp the fundamental characteristics and core structures of Bibliodrama, which has recently received a lot of attention in various fields including Christian education. Bibliodrama is a guided form of process-oriented staging of biblical texts in groups with the aim of mutually opening up the text and the biography of the participants in the implementation of holistic encounters (H. Aldebert). In the background of the birth of Bibliodrama can be found the hermeneutical efforts of the biblical scholar Walter Wink who sentenced the bankruptcy of historical criticism. He laid the biblical interpretative foundation for Bibliodrama which combines Bible and body. German theologian Gerhard Marcel Martin had a new experience of seeing the Bible through body activity during his life in New York, and based on that experience he began to work on the Bibliodrama. And the New Testament scholar Tim Schramm, who focused on the TCI (themecentered interaction) movement, found the optimal methodology to embody the interaction of biblical studies in Bibliodrama. On the other hand, Peter Pitzele, who wanted to realize the Bibliodrama in the tradition of Midrash, has developed a new type of Bibliodrama (Bibliolog) that is different from the European Bibliodrama. When we put together the positions of the pioneers of Bibliodrama, it turns out that it has three fundamental characteristics: body, interaction, and the empty space of the Bible. The body refers to the personality of learners participating in the Bibliodrama. They are not passive participants, but voluntary and active participants. Interaction is realized through the dramatic way of Bibliodrama. Bibliodrama aims for a dynamic process in which hermeneutical interaction occurs. The empty space of the Bible, which Bibliodrama pays attention to, allows us to understand why the Bible is not a fixed word but a living word that is still heard today. In order to understand the Bible as the content of education, Bibliodrama liberates the text that is fixed in a literal way and gives life by paying attention to the empty space of the Bible and reading it slowly.

Villagers' Participation in Conservation of Village Woodlands -Two cases of Namwon City, Korea (마을숲 보전 사업에 대한 마을 주민의 참여가능성 -남원시 2개 마을의 경우)

  • Park, So-Hee;Koo, Ja-Choon;Youn, Yeo-Chang
    • Journal of Korean Society of Forest Science
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    • v.102 no.1
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    • pp.15-23
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    • 2013
  • This study aims to analyze factors affecting villagers' participation in conservation of village woodlands. We postulated that socio-economic characteristics such as gender, age, place of birth, wealth level, frequency of use and leader experience could contribute to decision-making of participation in the village woodland conservation. Data were collected by interviewing 52 villagers in village A and 41 villagers in village B and were used to estimate the probability function of villager's participation in village woodland conservation using ordered logit model. The results indicate that frequent use of village woodlands increases the probability of villagers' participation in conservation of village woodlands in the case of village A. In the case of village B, people who were born in the village and have experiences of serving the village as a village leader are more likely to participate in the village woodland conservation activities. Considering the fact that the village woodland of village A is owned by the community as a village common, and that cultural activities remain in the village woodland of village A, the utility of village woodlands to the residents and their common understanding of village woodlands seem to influence the likelihood of villagers' participation in conservation of village woodlands. In order to induce villagers' participation in the village woodland conservation, it is necessary to recognize the right of villager's access and provide villagers with common understanding of village woodlands through cultural activities and education programs.

An Empirical Study on the Clustering Measurement and Trend Analysis among the Asian Ports Using the Context-dependent and Measure-specific Models (컨텍스트의존 모형 및 측정특유 모형을 이용한 아시아항만들의 클러스터링 측정 및 추세분석에 관한 실증적 연구)

  • Park, Ro-Kyung
    • Journal of Korea Port Economic Association
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    • v.28 no.1
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    • pp.53-82
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    • 2012
  • The purpose of this paper is to show the clustering trend by using the context-dependent and measure-specific models for 38 Asian ports during 10 years(2001-2009) with 4 inputs and 1 output. The main empirical results of this paper are as follows. First, clustering results by using context-dependent and measure-specific models are same. Second, the most efficient clustering was shown among the Hong Kong, Singapore, Ningbo, Guangzhou, and Kaosiung ports. Third, Port Sultan Qaboos, Jeddah, and Aden ports showed the lowest level clustering. Fourth, ranking order of attractiveness is Guangzhou, Dubai, HongKong, Ningbo, and Shanghai, and the results of progressive scores confirmed that low level ports can increase their efficiency by benchmarking the upper level ports. Fifth, benchmark share showed that Dubai(birth length), and HongKong(port depth, total area, and no. of cranes) have affected the efficiency of the inefficient ports.

The Relationship between Perceived Parent-Child Relationship, Parent-Child Attachment and Self-Concept of Woman College Students (여대생이 지각한 부모-자녀 관계가 부모-자녀 애착과 자아개념에 미치는 영향)

  • Moon, Young-Sook;Park, In-Sook
    • Korean Parent-Child Health Journal
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    • v.8 no.1
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    • pp.75-88
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    • 2005
  • Purpose: This study was to examine the relationship between perceived the past parent-child relationship(caring and overprotection), present parent-child attachment (communication, confidence, alienation) and self-concept of woman college students. Method: The data was collected from March 15 through 26, 2004. The subjects in this study were 395 students who attended universities located in N and T city. The data analyzed using with SAS program, and analysis of variance, Pearson correlation analysis, multiple regression. Results: Concerning the connection of demographic variables to the past parent-child relations(caring and overprotection), current parent-child attachment(communication, confidence, alienation) made a Significant difference in economic family standard (p<.05), educational level of their fathers(p<.01), birth of order(p<.05) and whether or not they lived with their parents under the same roof(p<.05), past experience of living with their parents under the same roof(p<.01). The relationship of the past parent-child relations(caring and overprotection), and current parent-child attachment(communication, confidence, alienation) made a significant difference to communication(p<.001), confidence(p<.001), alienation(P<.001). The correlation among the past parent-child relations(caring and overprotection), current parent-child attachment(communication, confidence, alienation) and self-concept, caring had significantly positive correlation(p<.001) with communication(r=0.631), confidence(r=0.669) and self-concept(r=0.520), and had significantly negative correlation(p<.001) with overprotection(r=-0.369), alienation(r=-0.531). The overprotection had Significantly negative correlation(p<.001) with communication(r=-0.303) confidence(r=-0.369) and self-concept(r=-0.292), and that had significantly positive correlation(p<.001) with alienation (r=0.275). Overall, the past parent child relations(caring and overprotection) made a 28.2% prediction of self-concept, and the current parent-child attachment(communication, confidence and a sense or alienation) made a 46.1% prediction or self-concept, and their predictability was all significant. Conclusion: This study suggests that perceived the past parent-child relationship(caring and overprotection) is significant influencing factors on present parent-child attachment(communication, confidence, alienation), self-concept. Therefore, nursing strategy is needed to manage these revealed factors.

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Studies on the Effects of Ginseng Extract for Mucociliary Change in Mice Nasal Septum Epithelia Exposured to Sulfur Dioxide gas (인삼(人蔘) 추출액(抽出液)이 SO2 Gas에 폭로(暴露)된 새앙쥐 호흡기상피(呼吸器上皮)의 섬모(纖毛)변화에 미치는 영향(影響)에 관한 연구(硏究))

  • Kim, Moo-Kang;Cho, Sung-Whan;Ryu, Si-Yun;Lee, Geun-Jwa;Han, Kyong-O;Lee, Chul-Ho
    • Korean Journal of Veterinary Research
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    • v.31 no.3
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    • pp.241-251
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    • 1991
  • In order to investigate the effect of Panax ginseng extract and it's degree in mucociliary change of mice nasal septum epithelia exposured to sulfur dioxide, 96 ICR male mice were used. They were at first divided the 4th week, the 8th week or the 16th week groups according to the age after birth and 6 hour or 12 hour groups according to the $SO_2$ gas exposured hour in a day, and at control, 50mg, 100mg and 200mg injection groups according to the dosage of the freeze-drying powder of the ginseng extract which was injected into the mouse peritoneal cavities in the condition of the solution solved with physiological saline solution. Each subgroups which were divided finaly included 4 male mice. The histological tissue sections for observation were made from nasal septum, posterior nasal orfice and trachea. The results obtained by experiments were summarized as followings. 1. The loss of the nasal mucosa epithelial cilia of the mouse exponsure to the $SO_2$ gas after ginseng extract injection was apparently diminish eompare to those exposured only $SO_2$ gas without pretreatment of ginseng extract (p<0.01). 2. The inhibition effect for the loss of nasal mucocilia according to the ginseng extract dosages not found in this research (p>0.05). 3. There were differences in the loss of nasal mucosa cilia according to the $SO_2$ gas exposure time between the control group and ginseng extract pretreatment group (p<0.01). 4. According to the increase of the postnatal time, there were remarkable differences between the control group and the ginseng extract pretreatment groups in the loss of nasal mucosa cilia (p<0.01). 5. Ciliary changes of the posterior nasal orifice and trachea according to the $SO_2$ gas exposure time, mice age and ginseng dosages, were not dearly observed in this light microscopical observation.

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The Effect of Positioning on the Hand Function and Correlation of Variable in Children with Cerebral Palsy(Spastic Diplegia) (자세변화에 대한 뇌성마비아동(경직성 양마비)의 손 기능 차이 비교와 제변수와의 상관관계의 연구)

  • Jung, Min-Ye;Current, Marion E.;Kwon, Hyuk-Cheol
    • Physical Therapy Korea
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    • v.3 no.3
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    • pp.44-58
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    • 1996
  • The purpose of this study was to compare and evaluate various hand functions in the most common position (chair sitting, standing, floor sitting) used by cerebral palsied children with spastic diplegia. The results, analysed statistically, could be useful in suggesting treatment strategy for the improvement of hand function in such patient. For this study, 27 children mild or moderate spastic diplegia were chosen. They were patients of the Rehabilitation Hospital, Yonsei University Medical Center. Both dominant and nondominant hands were tested by the Box and Block Test. Bilateral hand function was tested by bead striding and card sorting activities. Collected data was analysed using univariate correlation analysis and MANOVA. Results were as follows: 1) In chair sitting there was a significant positive correlation between dominant hand scores in the Bloc and Box Test and chronological age, gestation period, and time of treatment initiation. In bilateral hand function, card sorting scores correlated positively with time of treatment initiation. 2) In standing, there was a significantly positive correlation between dominant hand scores in the Block and Box Test and time of treatment initiation. 3) In floor sitting, there was a significantly positive correlation between the dominance hand scores in the Block and Box Test and the tine of treatment initiation. Bead stringing, a bilateral hand activity, correlated positively with gestation period and birth weight but negatively with the postnatal incubation period. 4) That score of children who walked showed no significant difference in any of the three postures. 5) Highest test scores in children who could nat walk were in the Box and Block Test for nondominant hand in bead stringing for bilateral hand function. There scores occurred with the children in thee chair sitting posture. The results showed that, in order to improve hand function in children with spastic diplegia, it is necessary to maintain a well supported upright trunk posture with variations allowed for relevance to the chosen position of thee improvements hand activity being performed.

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Female worker′s menstrual discomforts and coping -focus on hospital workers- (여성근로자의 월경시 불편감과 대처방법에 따른 완화정도 -의료기관 종사자를 대상으로-)

  • Lim, Yun-Mi
    • Journal of the Korea Convergence Society
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    • v.9 no.2
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    • pp.259-266
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    • 2018
  • Purpose: This study is to investigate the pattern of discomfort, coping style and relief level of female workers. Methods: The subjects were 394 women who worked at three general hospitals located in S city and agreed to the purpose of this study. Data were collected April 20~30, 2010, and one-way ANOVA and Pearson's correlation were performed using descriptive statistics using SAS 9.2. Results: showed that discomfort during menstruation was significantly different from age, marital status, pregnancy experience, and birth experience. The average degree of discomfort during menstruation was $2.46{\pm}0.68$, which was the average of 5, and the pattern was pain, water accumulation and behavior change. The degree of mitigation according to coping strategies was the most effective at $3.55{\pm}0.58$, and the relationship between coping behavior and discomfort during menstruation was correlated only with avoidant coping. p = .001). Conclusion: In order to mitigate the inconvenience of menstruation, women should seek to cope with their own coping strategies rather than passive coping methods and suggest the development of an educational program that can relieve the discomfort during menstruation.

A study of college students who were granted special admissions for vocational high school students (대학입학전형에서 동일계 특별전형 합격생들에 대한 연구)

  • Shin, Jae-Kyoung
    • Journal of the Korean Data and Information Science Society
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    • v.24 no.4
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    • pp.763-771
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    • 2013
  • Due to a decrease in the number of college prep students which was triggered by the low birth rate since 1990, it is predicted, beginning 2016, that the entrance quota for colleges will exceed the number of applicants for college admissions. This serious imbalance between supply and demand raises serious problems for the regional universities, many of which have already been struggling to recruit new students and even considered closing down the schools. In an effort to securing best high school students, many of the schools have been developing various unique and specialized selectional processes for the applicants. In this research, through a statistical analysis of special admission processes for vocational high school students who were admitted to a regional state-run university (University A), I tried to find a way to effectively deal with the student management. Statistically analyzing these first-year students' enrollment and their adjustment processes as well as the departmental relationships and gender differences, I tried to find some better ways to secure good applicants. It is expected that this study will not only be utilized as guide to adopt a way to bypass the enrollment quota in order to secure talented students but also be served as a reference that will help the students adapt themselves to school life until they graduate. It is also expected that this study will be expanded in such a way as to compare it with other models and even develop a new type of college entrance system that would fit future college admission policies.