• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.175-175
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• 2005

• ETRI Journal
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• v.34 no.3
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• pp.439-449
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• 2012

In recent innovation trends, one notable feature is the merging and overlapping of technologies: in other words, technological convergence. A key technological convergence is the fusion of biotechnology (BT) and information technology (IT). Major IT advances have led to innovative devices that allow us to advance BT. However, the lack of data on IT-BT convergence is a major impediment: relatively little research has analyzed the inter-disciplinary relationship of different industries. We propose a systematic approach to analyzing the technological convergence of BT and IT. Patent analysis, including citation and co-classification analyses, was adopted as a main method to measure the convergence intensity and coverage, and two portfolio matrices were developed to manage the technological convergence. The contribution of this paper is that it provides practical evidences for IT-BT convergence, based on quantitative data and systematic processes. This has managerial implications for each sector of IT and BT.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.317-324
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• 2009

The insecticidal toxin gene of Bacillus thuringiensis (Bt) is one of the most commonly used in the development of genetically modified (GM) crops. In this research, we analyzed Bt rice showing lepidopteran pest-resistance. The Bt gene is a synthetic Cry1Ac composed of optimal codons for plants, and the Bt protein is targeted to the chloroplast by a transit peptide. Three Cry1Ac rice events (C103-3, C127-1, and C7-1) were analyzed for molecular characterization. C103-3 contains two copies of T-DNA where the left border (LB) region is truncated. Both C7-1 and C127-1 have a single copy of T-DNA, but a part of the vector backbone DNA is inserted into the genome of C127-1; thus, only C7-1 had intact T-DNA. Progenies of C7-1 crossed with the original cultivar, Nakdong, and double-haploid lines from anther culture of lines crossed with the elite cultivar, Dongjin, were analyzed for T-DNA flanking genomic DNA and genotyping. Results showed that an intact T-DNA region without the vector backbone was inserted into the genome and was stably inherited through generations. The C7-1 homozygous event could be used as breeding material to develop GM rice with pest resistance.

• Electrical & Electronic Materials
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• v.17 no.4
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• pp.32-39
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• 2004

차세대 신기술로 부상하고 있는 나노바이오 기술(Nano-Bio Technology)은 10억분의 1이라는 정밀도를 바탕으로 원자나 분자를 조작해 새로운 물질을 만들고 시스템을 창조하는 나노기술(Nanotechnology : NT)과 생명현상을 연구 대상으로 하는 바이오 기술 (Biotechnology : BT)이 융합된 분야이다. 근본적으로 무기물을 다루는 NT와 생명체를 다루는 BT가 융합할 수 있는 것은 BT의 궁극적인 연구대상인 DNA, RNA, 단백질의 크기가 수 나노미터로 NT의 연구대상의 크기와 동일하다는 데에 있다.(중략)

• 한국IT서비스학회:학술대회논문집
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• 2003.05a
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• pp.428-435
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• 2003

Post-Genome 시대의 Biotechnology(BT) 산업에 세계적으로 관심과 투자가 증가하고 있다. BT 산업은 기술 및 지식 집약적 특성상 R&D 활동이 중요하기에 BT 연구실의 실험 생산성 증가에 대한 연구가 필요하다. 이와 관련하여 연구실 지식경영과 지식경영시스템활용에 관한 연구가 활발히 진행되고 있다. 하지만 그 초점이 연구 지식들의 조직화, 체계화 및 검색기능 등의 원활한 지식 공급 시스템 구축에 있기에 효율적이고 효과적인 지식 생성 방안을 제시해 주지 못하고 있다. 이에 본 연구는 실험실 내 데이터 처리 및 통합 관리 역할을 해온 LIMS(Laboratory Information Management System)를 활용하여 실험 데이터들로부터 실험 디자인을 위한 효율적이고 효과적인 형식지(Explicit Knowledge)의 생성을 목적으로 하는 지식 생성 툴(Knowledge Creation Tool)을 설계하였다. 또한 상기 모델을 적용하여 실제 어떠한 형태로 지식이 생성되는지 A대학교 B유전자 연구실의 실험 데이터로 실증 분석해 보았다.

• Journal of Microbiology and Biotechnology
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• v.25 no.5
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• pp.658-661
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• 2015

Genes encoding enzymes with sequence similarity to hopanoids biosynthetic enzymes of other organisms were cloned from the hopanoid (hop) gene cluster of Streptomyces peucetius ATCC 27952 and transformed into Streptomyces venezuelae YJ028. The cloned fragments contained four genes, all transcribed in one direction. These genes encode polypeptides that resemble polyprenyl diphosphate synthase (hopD), squalene-phytoene synthases (hopAB), and squalene-hopene cyclase (hopE). These enzymes are sufficient for the formation of the pentacyclic triterpenoid lipid, hopene. The formation of hopene was verified by gas chromatography/mass spectrometry.

• Journal of Microbiology and Biotechnology
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• v.4 no.1
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• pp.56-62
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• 1994

A yeast strain, BT001, which can directly ferment D-xylose to ethanol was isolated from forest soils, and then identified as Candida sp. Cultural conditions for the optimum ethanol production, along with the effects of aeration on cell growth and ethanol production were investigated. Aeration stimulated the cell growth and the volumetric rate of ethanol production, but decreased the ethanol yield. Optimum temperature and initial pH for the ethanol production were $33{\circ}^C$ and 6.0, respectively. In a shake flask culture, this strain produced 52.3 g ethanol per liter from 12%(w/v) D-xylose after incubation for 96 hours. Ethanol yield was 0.436 g per g D-xylose consumed. This corresponds to 85.8% of theoretical yield. Also, this yeast strain produced ethanol from D-galactose, D-glucose and D-mannose, but not from L-arabinose and L-rhamnose. Among these sugars, D-glucose was the fastest in being converted to ethanol sugars.

• Journal of Life Science
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• v.17 no.5 s.85
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• pp.658-663
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• 2007

Bacillus thuringiensis is a well-known species of entomophathogenic bacteria that is widely used as a biopesticide against many insect pests. It produces parasporal crystals ($\delta$-endotoxin) and endospores during sporulation. In this report, the $\delta$-endotoxin produced by Bacillus thuringiensis BT-1 and BT-2 were characterized by Scanning Electron Microscope(SEM), Transmission Electron Microscope(TEM), SDS-PACE, and solubilization activity by alkaline solution. BT-1, BT-2 were cultured in the GBY medium, and the $\delta$-endotoxin of them was purified with discontinuous sucrose density gradient centrifugation. Their $\delta$-endotoxin was observed by SEM and TEM. Morphologically, the $\delta$-endotoxin of BT-1 was a square and flat type, whose size was $1.73{\mu}m{\times}0.7{\mu}m$, and the $\delta$-endotoxin of the BT-2 was spherical form whose size was $1.1{\mu}m{\times}0.9{\mu}m$ determined by SEM and TEM. The $\delta$-endotoxin of the BT-1 was composed of 28 kDa and 21 kDa, however, it of the BT-2 was composed of 50 kDa, 35 kDa, and 22 kDa bands determined by SDS-PACE. The purified crystals of BT-1 and BT-2 were dissolved gradually in alkaline solution as time goes by, and it was perfectly dissolved after 3 hours. It is supposed that the $\delta$-endotoxin of crystal was converted to a state of activation in the course of time in the intestines of insect.

• BT NEWS
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• v.13 no.3
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• pp.24-32
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• 2006

• BT NEWS
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• v.12 no.3
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• pp.18-27
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• 2005