• Title/Summary/Keyword: Biophotonics

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Chemometric Analysis of 2D Fluorescence Spectra for Monitoring and Modeling of Fermentation Processes (생물공정 모니터링 및 모델링을 위한 2차원 형광스펙트럼의 다변량 분석)

  • Kang Tae-Hyoung;Sohn Ok-Jae;Kim Chun-Kwang;Chung Sang-Wook;Rhee Jong-Il
    • KSBB Journal
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    • v.21 no.1 s.96
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    • pp.59-67
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    • 2006
  • 2D spectrofluorometer produces many spectral data during fermentation processes. The fluorescence spectra are analyzed using chemometric methods such as principal component analysis (PCA), principal component regression (PCR) and partial least square regression (PLS). Analysis of the spectral data by PCA results in scores and loadings that are visualized in score-loading plots and used to monitor a few fermentation processes by S. cerevisae and recombinant E. coli. Two chemometric models were established to analyze the correlation between fluorescence spectra and process variables using PCR and PLS, and PLS was found to show slightly better calibration and prediction performance than PCR.

Development of Optical Fiber Glucose and Lactate Biosensors for Bioprocess Monitoring (생물공정 모니터링을 위한 광섬유 포도당 및 젖산 센서의 개발)

  • Jung, Chang Hwan;Sohn, Ok-Jae;Rhee, Jong Il
    • KSBB Journal
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    • v.32 no.1
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    • pp.35-45
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    • 2017
  • In this work the optical fiber glucose and lactate biosensors were developed by using fluorescent dye and enzyme immobilized on the end tip of an optical fiber. 3-Glycidyloxypropyl)methyldiethoxysilane (GPTMS), (3-Aminopropyl) trimethoxysilane (APTMS) and Methyltrimethoxysilane (MTMS) were used to immobilize glucose oxidase (GOD), lactate oxidase (LOD) and ruthenium(II) complex (tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II), $Ru(dpp)_3^{2+}$) as oxygen sensitive fluorescent dye. MTMS sol-gel was an excellent supporting material for the immobilization of $Ru(dpp)_3^{2+}$, GOD, and LOD on the optical fiber. Storage stability of the optical fiber glucose sensor was kept constant over 20 days, while the optical fiber lactate sensor had constant storage stability over 17 days. The optical fiber glucose and lactate biosensors also maintained good operational stability for 20 hours and 14 hours, respectively. The activities of the immobilized enzymes were most excellent at pH 7 and at $25^{\circ}C$. On-line monitoring of glucose and lactate in a simulated process was performed with the optical fiber glucose and lactate biosensors. On-line monitoring results were agreed with those of off-line data measured with high performance liquid chromatography (HPLC).

Characterization of Double-layer Optical Sensing Membranes for Dual Sensing of Dissolved Oxygen Concentrations and pH and Their Application to the On-line Monitoring of Fermentation Processes (용존산소농도와 pH의 동시 검출용 이중층 광학 센서막의 특성 및 발효공정의 온라인 모니터링에의 응용)

  • Kim, Chun-Kwang;Lee, Jong-Il
    • Applied Chemistry for Engineering
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    • v.20 no.4
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    • pp.370-374
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    • 2009
  • A double-layer optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen (DO) and pH value simultaneously. (tris(4,7-diphenyl-1,10-phenanthroline (Rudpp) ruthenium(II)) as a DO sensitive dye has been mixed in the methyl trimethoxy silane (MTMS) sol-gel solution and coated onto one well in a 24-well microtiter plate. On the DO-sensing layer the GA (3-glycidoxypropyl trimethoxy silane (GPTMS), 3-aminopropyl trimethoxy silane (APTMS)) sol-gel solution mixed with 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) has been coated and used to measure pH values. The double-layer sensing membrane was affected by ionic strength and temperature. The double-layer sensing membrane for DO and pH has been applied to online monitor in microorganism cultivation processes and showed a good performance.

Analysis of Two-Dimensional Fluorescence Spectra in Biotechnological Processes by Artificial Neural Networks I - Classification of Fluorescence Spectra using Self-Organizing Maps - (인공신경망에 의한 생물공정에서 2차원 형광스펙트럼의 분석 I - 자기조직화망에 의한 형광스펙트럼의 분류 -)

  • Lee Kum-Il;Yim Yong-Sik;Kim Chun-Kwang;Lee Seung-Hyun;Chung Sang-Wook;Rhee Jong Il
    • KSBB Journal
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    • v.20 no.4
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    • pp.291-298
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    • 2005
  • Two-dimensional (2D) spectrofluorometer is often used to monitor various fermentation processes. The change in fluorescence intensities resulting from various combinations of excitation and emission wavelengths is investigated by using a spectra subtraction technique. But it has a limited capacity to classify the entire fluorescence spectra gathered during fermentations and to extract some useful information from the data. This study shows that the self-organizing map (SOM) is a useful and interpretative method for classification of the entire gamut of fluorescence spectral data and selection of some combinations of excitation and emission wavelengths, which have useful fluorometric information. Some results such as normalized weights and variances indicate that the SOM network is capable of interpreting the fermentation processes of S. cerevisiae and recombinant E. coli monitored by a 2D spectrofluorometer.

Analysis of Two-Dimensional Fluorescence Spectra in Biotechnological Processes by Artificial Neural Networks II - Process Modeling using Backpropagation Neural Network - (인공신경망에 의만 생물공정에서 2차원 영광스펙트럼의 분석 II - 역전파 신경망에 의한 공정의 모델링 -)

  • Lee Kum-Il;Yim Yong-Sik;Sohn Ok-Jae;Chung Sang-Wook;Rhee Jong Il
    • KSBB Journal
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    • v.20 no.4
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    • pp.299-304
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    • 2005
  • A two-dimensional (2D) spectrofluorometer was used to monitor various fermentation processes with recombinant E. coli for the production of 5-aminolevulinic acid (ALA). The whole fluorescence spectral data obtained during a process were analyed using artificial neural networks, i.e. self-organizing map (SOM) and feedforward backpropagation neural network (BPNN).Based on the classified fluorescence spectra a supervised BPNN algorithm was used to predict some of the process parameters. It was also shown that the BPNN models could elucidate some sections of the process performance, e.g. forecasting the process performance.

Photobiomodulation Mediated by Red and Infrared Light: A Study of Its Effectiveness on Corneal Epithelial Cells and Wound Healing (적색 및 적외선 빛을 이용한 Photobiomodulation: 각막상피세포에 대한 효과와 상처 치유에 관한 연구)

  • Sun Hee Ahn;Jae Sung Ahn;Byeongil Lee
    • Korean Journal of Optics and Photonics
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    • v.34 no.2
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    • pp.45-52
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    • 2023
  • In this study, we have investigated the effect of photobiomodulation (PBM) on corneal wound healing, using a low-power light-emitting diode (LED) at different wavelengths. We found that LEDs with wavelengths ranging from 623 to 940 nm had no significant cytotoxic effects on corneal epithelial cells. The effect of PBM on promoting cell migration was analyzed by scratch assay, and it was found that PBM at 623 nm significantly increased cell migration and promoted wound healing. Furthermore, the expression of genes related to cell migration and wound healing was analyzed, and it was found that PBM at 623 nm upregulated the expression of the genes FGF-1 and MMP2, which are known to promote cell proliferation and extracellular matrix degradation. These findings suggest that PBM with low-powered light at specific wavelengths, particularly 623 nm, could be utilized to treat corneal injury.