Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Characterization of Double-layer Optical Sensing Membranes for Dual Sensing of Dissolved Oxygen Concentrations and pH and Their Application to the On-line Monitoring of Fermentation Processes

용존산소농도와 pH의 동시 검출용 이중층 광학 센서막의 특성 및 발효공정의 온라인 모니터링에의 응용

  • Kim, Chun-Kwang (Department of Material and Biochemical Engineering, Chonnam National University,Research Center for Biophotonics, Chonnam National University,Center for Functional Nano Fine Chemicals, Chonnam National University) ;
  • Lee, Jong-Il (School of Applied Chemical Engineering, Chonnam National University,Research Institute for Catalysis, Chonnam National University,Research Center for Biophotonics, Chonnam National University,Center for Functional Nano Fine Chemicals, Chonnam National University)
  • 김춘광 (전남대학교 물질.생물화학공학과,전남대학교 바이오광사업단,전남대학교 기능성나노정밀화학사업단) ;
  • 이종일 (전남대학교 응용화학공학부,전남대학교 촉매연구소,전남대학교 바이오광사업단,전남대학교 기능성나노정밀화학사업단)
  • Published : 2009.08.10
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Abstract

A double-layer optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen (DO) and pH value simultaneously. (tris(4,7-diphenyl-1,10-phenanthroline (Rudpp) ruthenium(II)) as a DO sensitive dye has been mixed in the methyl trimethoxy silane (MTMS) sol-gel solution and coated onto one well in a 24-well microtiter plate. On the DO-sensing layer the GA (3-glycidoxypropyl trimethoxy silane (GPTMS), 3-aminopropyl trimethoxy silane (APTMS)) sol-gel solution mixed with 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) has been coated and used to measure pH values. The double-layer sensing membrane was affected by ionic strength and temperature. The double-layer sensing membrane for DO and pH has been applied to online monitor in microorganism cultivation processes and showed a good performance.

용존산소농도(DO)와 pH를 동시에 검출하기 위하여 이중층 광학 센서 막을 제조하였다. DO 민감성 염료인 Rudpp를 MTMS 졸-겔에 혼합하고 24-웰 마이크로타이터 플레이트에 코팅하였다. DO-검출층 위에 HPTS와 혼합된 GA 졸-겔용액을 코팅하고 pH 측정을 위해 사용하였다. 이중층 광학 센서 막은 온도와 이온 강도에 영향을 받았다. 또한 DO와 pH 이중층 광학 센서 막은 미생물발효공정에 온라인 모니터링 하는데 응용하였으며 좋은 성능을 보였다.

Keywords

Acknowledgement

Supported by : 지식경제부

References

  1. S. Nagl and O. S. Wolfbeis, Analyst, 132, 507 (2007) https://doi.org/10.1039/b702753b
  2. A. S. Kocincova, S. M. Borisov, C. Krause, and O. S. Wolfbeis, Anal. Chem., 79, 8486 (2007) https://doi.org/10.1021/ac070514h
  3. S. M. Borisov, C. Krause, S. Arain, and O. S. Wolfbeis, Adv. Mater., 18, 1511 (2006) https://doi.org/10.1002/adma.200600120
  4. B. Zelelow, G. Khalil, G. Phelan, B. Carlson, M. Gouterman, J. B. Callis, and L. R. Dalton, Sens. Actuators B-Chem., 96, 304 (2003) https://doi.org/10.1016/S0925-4005(03)00547-1
  5. S. M. Borisov, G. S. Vasylevska, C. Krause, and O. S. Wolfbeis, Adv. Funct. Mater., 16, 1536 (2006) https://doi.org/10.1002/adfm.200500778
  6. K. P. McNamara, X. Li, A. D. Angela, and Z. Rosenzweing, Anal. Chim. Acta, 361, 73 (1998) https://doi.org/10.1016/S0003-2670(97)00703-4
  7. A. C. Tizzard, J. H. Bergsma, and G. Lloyd-Jones, Biosen. & Bioelectro., 22, 759 (2006) https://doi.org/10.1016/j.bios.2006.01.011
  8. Y. Amao and I. Okura, Sens. Actuators B-Chem., 88, 162 (2003) https://doi.org/10.1016/S0925-4005(02)00320-9
  9. H. R. Kermis, Y. Kostov, and G. Rao, Analyst, 128, 1181 (2003) https://doi.org/10.1039/b304902g
  10. D. A. Nivens, M. V. Schiza, and S. M. Angel, Talan., 58, 543 (2002) https://doi.org/10.1016/S0039-9140(02)00323-5
  11. M. E. Koese, B. F. Carrol, and K. S. Schanze, Langmuir, 21, 9121 (2005) https://doi.org/10.1021/la050997p
  12. C.-K. Kim, O.-J. Sohn, and J. I. Rhee, Proc. Korean Sensors Conference, 77, Chosun University, Gwangju, Korea (2007)
  13. C.-K. Kim, O.-J. Sohn, and J. I. Rhee, Proc. 2008 Spring KSBB meeting and Intern. Symp., 96, Chonbuk Nationnal University, Jeonju, Korea (2008)
  14. P. Harms, Y. Kostov, J. A. French, M. Soliman, A. Anjanappa, A. Ram, and G. Rao, Biotechnol. Bioeng., 93, 6 (2006) https://doi.org/10.1002/bit.20742
  15. F. Kensy, G. T. John, B. Hofmann, and J. Buchs, Bioprocess Biosyst. Eng., 75, 75 (2005)
  16. Y. J. Tang, D. Laidlaw, K. Gani, and J. D. Keasling, Biotechnol. Bioeng., 95, 176 (2006) https://doi.org/10.1002/bit.21002