• 한국재료학회지
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• 제15권11호
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• pp.690-696
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• 2005

The ultrastructure ore of a nanostructured apatite film nucleated from solution was studied to gain insights into that of bone minerals which is the most important constituent to sustain the strength of bones. Needle-shaped apatite crystal plates with a bimodal size distribution $(\~100\;to\;\~1000 nm)$ were randomly distributed and they were found to grow parallel to the c-axis ([002]), driven by the reduction of surface energy. Between these randomly distributed needle-shaped apatite crystals which are parallel to the film, apatite crystals (20-40nm) with the normal of the grains quasi-perpendicular to the c-axis were observed. These observations suggest that the apatite film is the interwoven structure of apatite crystals with the c-axis parallel and quasi-perpendicular to the fan. In some regions, amorphous calcium phosphate, which is a precursor of apatite, was also observed. In the amorphous phase, small crystalline particle with the size of 2-3 nm were observed. These particles were quite similar, in size and shape, to those observed in the femoral trabecular bone, suggesting the nucleation of apatites by a biomimetic process in vitro is similar to that in vivo.

• 로봇학회논문지
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• 제19권2호
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• pp.221-228
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• 2024

As the importance of cardiovascular health is highlighted, research on its correlation with blood pressure, the most important indicator, is being actively conducted. Therefore, extensive clinical data is essential, but the measurement of the central arterial blood pressure waveform must be performed invasively within the artery, so the quantity and quality are limited. This study suggested a mock circulatory robot and artificial aorta to reproduce the blood pressure waveform generated by the overlap of forward and reflected waves. The artificial aorta was fabricated with biomimetic silicone to mimic the physiological structure and vascular stiffness of the human. A pressurizing chamber was implemented to prevent distortion of the blood pressure waveform due to the strain-softening of biomimetic silicone. The reproduced central arterial blood pressure waveforms have similar magnitude, shape, and propagation characteristics to humans. In addition, changes in blood pressure waveform due to aging were also reproduced by replacing an artificial aorta with various stiffness. It can be expanded to construct a biosignal database and health sensor testing platform, a core technology for cardiovascular health-related research.

• Tribology and Lubricants
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• 제28권6호
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• pp.297-302
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• 2012

We conducted 3D elastic contact analysis of a sharkskin surface pattern for the characteristic assessment of biomimetic shark skin structure pattern for engineering applications. Rough sharkskin surfaces of similar size with real shark skin scales are generated numerically. Under the assumption of the contact of a plate on the generated sharkskin surface, contact performances such as contact pressure, real contact area, and surface separation are evaluated.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.229.2-230
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• 2003

Extracellular matrix(ECM) is composed of the ground materials(proteoglycan) and nano size diameter fibrous proteins(ex. collagens) that together form a composite-like structure. In this study, fibrous scaffold with biomimetic architecture based on collagen nanofibers interpenetrated in PLGA/chitosan microfibrous matrix. Chitosan was selected for its structure similarity to glycosaminoglycan and neutralizing capacity for PLGA acidic metabolite. Collagen nanofiber were prepared by electrospinning. (omitted)

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.397-397
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• 2010

Bone is considered as hierarchically organized biocomposites of organic (collagen) and inorganic (hydroxyapatite) materials. The precise structural dependence between hydroxyapatite (HAp, $Ca_{10}(PO_4)_6(OH)_2)$ crystals and collagen fibril is critical to unique characteristics of bone. To meet those conditions and obtain optimal properties, it is essential to understand and control the initial growth mechanisms of hydroxyapatite at the molecular level, such as other nano-structured materials. In this study, collagen fibrils were prepared by adsorbing native type I collagen molecules onto hydrophobic surface. Hydrophobicity was introduced on the Si wafer surface by using PECVD (plasma enhanced chemical vapor deposition) method and cyclohexane as a precursor. Biomimetic nucleation and growth of HAp on the self-assembled collagen nanofibrils were occurred through incubation of the sample in SBF (simulated body fluid). Chemical and morphological evolution of HAp nanocrystals was investigated by surface-sensitive analytical techniques such as ToF-SIMS (Time-of-Flight Secondary Ion Mass Spectrometry) and AFM (Atomic Force Microscopy) in the early growth stages (< 24 hrs). The very initial stages (< 12 hrs) of mineralization could be clearly demonstrated by ToF-SIMS chemical mapping of surface. In addition to ToF-SIMS and AFM measurement, scanning electron microscopy, energy dispersive spectroscopy and X-ray diffraction analysis were conducted to characterize the HAp layer in the late stages. This study is of great importance in the growth of real bone-like materials with a structure analogous to that of natural bones and the development of biomimetic nanomaterials.

• Tribology and Lubricants
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• 제29권5호
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• pp.304-309
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• 2013

Shark skin has functionalities such as self-cleaning and antifouling; it also exhibits excellent drag reduction owing to a hierarchical structure of microgrooves and nanometer-long chain mucus drag reduction interfaces around the shark body. In this study, the wettability of a shark skin surface and its replicas are evaluated. First, a shark skin template is taken from a real shark. Then, shark skin replicas are produced directly from a shark skin template, using a micromolding technique. The quantitative replication precision of the shark skin replicas is evaluated by comparing the geometry of the shark skin template to the replica using 2D surface profiles. Contact angles at the solid-air-water interfaces are evaluated for the shark skin template and its replicas under two conditions: with and without hydrophobic coating. The results show that the microriblets on shark skin improve the hydrophobic feature and play a critical role in self-cleaning.

• The Korean Journal of Ceramics
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• 제5권3호
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• pp.265-269
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• 1999

Polyethylene was modified with silanol groups on its surface by photografting of vinyltrimethoxysilane in vapor phase by using benzophenon as a polymerization initiator and by hydrolyzing the methoxysilane groups into the silanol groups with HCI solution. The modified polyethylene formed a dense and homogeneous apatite layer on its surface in a solution with ion concentrations 1.5 times those of human blood plasma within 21 days. This kind of biomimetic process could provide techniques for fabricating apatite-polymer composites with three dimensional structure analogous to the natural bone.

• Journal of International Society for Simulation Surgery
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• 제3권2호
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• pp.49-59
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• 2016

3D printing is also known as additive manufacturing technique in which has been used in various commercial fields such as engineering, art, education, and medicine. The applications such as fabrication of tissues and organs, implants, drug delivery, creation surgical models using 3D printer in medical field are expanding. Recently, 3D printing has been developing for produce biomimetic 3D structure using biomaterials containing living cells and that is commonly called "3D bio-printing". The 3D bio-printing technologies are usually classified four upon printing methods: Laser-assisted printing, Inkjet, extrusion, and stereolithograpy. In the bio-printing, bio-inks (combined hydrogels and living cells) are as important components as bio-printing technologies. The presence of various types of bioinks, however, in this review, we focused on the bio-inks which enables bioprinting efficacy using hydrogels with living cells.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제33권4호
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• pp.301-307
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• 2011

Purpose: If the tooth structure is damaged, then it is impossible to regenerate the tooth. The materials used to restore the tooth structure are not related to the composition of the tooth. The materials used to restore the structure can't replace the natural tooth because they just fill the defective structure. Calcium phosphate cement remineralizes the dentin and almost replaces the natural tooth, but there are some disadvantages. We conducted basic tests with Biomimetic CPC (Bio-CPC) to make sure of the possibility of the biomaterial to remineralize the defective tooth structure. Methods: In this study, the bioactivity and biocompatibility of Bio-CPC were evaluated for its potential value as the bio-material for regeneration of damaged tooth structure by conducting a cell toxicity assay (WST-1 assay), a cytokinesis-block micronucleus assay, a chromosomal aberration test, total RNA extraction and RT-PCR on MDPC-23 mouse odontoblast-like cells. Results: The in vitro cytotoxicity test showed that the Bio-CPC was fairly cytocompatible for the MDPC-23 mouse odontoblast-like cells. Conclusion: Bio-CPC has a possibility to be a new biomaterial and further study of Bio-CPC is needed.

• 한국자기공명학회논문지
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• 제25권4호
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• pp.58-63
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• 2021

Apolipoprotein B-100 (apo B-100), the main protein component that makes up LDL (Low density lipoprotein), consists of 4,536 amino acids and serves to combine with the LDL receptor. The oxidized LDL peptides by malondialdehyde (MDA) or acetylation in vivo act as immunoglobulin (Ig) antigens and peptide groups were classified into 7 peptide groups with subsequent 20 amino acids (P1-P302). The biomimetic peptide P143 (IALDD AKINF NEKLS QLQTY) out of C-group peptides carrying the highest value of IgG antigens were selected for structural studies that may provide antigen specificity. Experimental results show that P143 has β-sheet in Ile[1]-Asn[9] and α-helice in Gln[16]-Tyr[20] structure. Homonuclear 2D-NMR (COSY, TOCSY, NOESY) experiments were carried out for NMR signal assignments and structure determination for P143. On the basis of these completely assigned NMR spectra and proton distance information, distance geometry (DG) and molecular dynamic (MD) were carried out to determine the structures of P143. The proposed structure was selected by comparisons between experimental NOE spectra and back-calculated 2D NOE results from determined structure showing acceptable agreement. The total Root-Mean-Square-Deviation (RMSD) value of P143 obtained upon superposition of all atoms were in the set range. The solution state P143 has a mixed structure of pseudo α-helix and β-turn(Phe[10] to Glu[12]). These results are well consistent with calculated structure from experimental data of NOE spectra. Structural studies based on NMR may contribute to the prevent oxidation studies of atherosclerosis and observed conformational characteristics of apo B-100 in LDL using monoclonal antibodies.