• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권3호
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• pp.1076-1094
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• 2022

Technology for emotion recognition is an essential part of human personality analysis. To define human personality characteristics, the existing method used the survey method. However, there are many cases where communication cannot make without considering emotions. Hence, emotional recognition technology is an essential element for communication but has also been adopted in many other fields. A person's emotions are revealed in various ways, typically including facial, speech, and biometric responses. Therefore, various methods can recognize emotions, e.g., images, voice signals, and physiological signals. Physiological signals are measured with biological sensors and analyzed to identify emotions. This study employed two sensor types. First, the existing method, the binary arousal-valence method, was subdivided into four levels to classify emotions in more detail. Then, based on the current techniques classified as High/Low, the model was further subdivided into multi-levels. Finally, signal characteristics were extracted using a 1-D Convolution Neural Network (CNN) and classified sixteen feelings. Although CNN was used to learn images in 2D, sensor data in 1D was used as the input in this paper. Finally, the proposed emotional recognition system was evaluated by measuring actual sensors.

• Molecules and Cells
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• 제44권7호
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• pp.529-537
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• 2021

Most animals face frequent periods of starvation throughout their entire life and thus need to appropriately adjust their behavior and metabolism during starvation for their survival. Such adaptive responses are regulated by a complex set of systemic signals, including hormones and neuropeptides. While much progress has been made in identifying pathways that regulate nutrient-excessive states, it is still incompletely understood how animals systemically signal their nutrient-deficient states. Here, we showed that the FMRFamide neuropeptide FLP-20 modulates a systemic starvation response in Caenorhabditis elegans. We found that mutation of flp-20 rescued the starvation hypersensitivity of the G protein β-subunit gpb-2 mutants by suppressing excessive autophagy. FLP-20 acted in AIB neurons, where the metabotropic glutamate receptor MGL-2 also functions to modulate a systemic starvation response. Furthermore, FLP-20 modulated starvation-induced fat degradation in a manner dependent on the receptor-type guanylate cyclase GCY-28. Collectively, our results reveal a circuit that senses and signals nutrient-deficient states to modulate a systemic starvation response in multicellular organisms.

• Animal cells and systems
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• 제6권3호
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• pp.221-225
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• 2002

This study was conducted to find out how young black-tailed gulls (Larus crassirostris) recognize adult voice signals after hatching. For the experiment, adult voice recorded in the natural environment was played back at controlled intervals and intensity (dB) to 15 young gulls that were artificially hatched in the laboratory. The chirirah call frequency of young gulls increased as the intensity of the mew call increased. The chirirah response of the control group was highest to the mew call at intervals of 1.8s. The adult long ca11 and alarm call also showed similar results to the mew call when the interval and intensity were manipulated similar to the mew call. Based on the results of this experiment, it is assumed that the young black-tailed gulls recognize adult voice signals based on the simple structure of adult voice signals, that is, the interval and intensity of the voice.

• Animal cells and systems
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• 제13권3호
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• pp.345-350
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• 2009

We have isolated and characterized a new insect chicken type (c-type) lysozyme gene from the common cutworm, Spodoptera litura. The full-length cDNA of Spodoptera lysozyme is cloned by rapid amplification of cDNA ends PCR (RACE-PCR). The isolated cDNA consists of 1039 bp including the coding region for a 142-amino acid residue polypeptide, which included a signal peptide of 21-amino acid residue and a mature protein of 121-amino acid residue. The predicted molecular weight of mature lysozyme and its theoretical isoelectric point from amino acid composition is 13964.8 Da and 9.05, respectively. The deduced amino acid sequence of Spodoptera lysozyme gene shows the highest similarity (96.7%) to Spodoptera exigua lysozyme among other lepidopteran species. Amino acid sequence comparison with other the c-type lysozymes, Spodoptera lysozyme has the completely conserved $Glu^{32}$ and $Asp^{50}$ of the active site and eight Cys residues are completely conserved in the same position as that of other lepidopteran lysozymes.

• Journal of Microbiology and Biotechnology
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• 제8권2호
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• pp.141-145
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• 1998

Overproduction of intracellular ${\beta}$-glucosidase was attempted by modifying the promoter region of a ${\beta}$-glucosidase gene cloned from Cellulomonas fimi and expressing it in Bacillus subtilis DB 104. A strong engineered promoter, BJ27UΔ88, was fused to the ${\beta}$-glucosidase gene after removing its native promoter. An effective Shine-Dalgamo sequence (genel0 of phage T7) was inserted between the promoter and the ${\beta}$-glucosidase structural gene. The modified gene was overexpressed in B. subtilis and produced 1121.5 units of ${\beta}$-glucosidase per mg protein which is about $12\%$ of total intracellular protein. Secretion of overproduced intracellular ${\beta}$-glucosidase was attempted by using the signal sequence of the Bacillus endoglucanase gene as well as an in-frame hybrid protein of endoglucanase. The hybrid protein was normally secreted into the culture medium and still retained ${\beta}$-glucosidase activity.

• Journal of Applied Biological Chemistry
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• 제50권2호
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• pp.58-62
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• 2007

Identification of Dual-specificity protein phosphatase (DSP) substrates is essential in revealing physiological roles of DSPs. We isolated VHZ-interacting proteins from extracts of 293T cells overexpressing a VHZ (C95S, D65A) mutant known to be substrate- trapping mutant. Analysis of specific proteins bound to VHZ by 2D gel electrophoresis and mass spectroscopy revealed that these proteins contained Chaperonin containing TCP1, Type II phosphatidylinositol phosphate kinase ${\gamma}$, Intraflagellar transport 80 homolog, and Kinesin superfamily protein 1B. VHZ-interacting proteins showed that VHZ is involved in many important cellular signal pathways such as protein folding, molecular transportation, and tumor suppression.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1985년도 춘계학술대회
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• pp.20-21
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• 1985

본 연구에서는 다중채널의 방대한 생체 신호 정보량을 전화회선을 이용하여, 효율적으로 전송하기 위한 생체 신호 데이터 전송 시스템을 설계한다. 특히 생체 신호 데이터를 신속하게 전송하기 위하여 가변어장 data frame을 사용하였고, 생체 신호 전처리 unit와의 interface 등을 검토하였다.

• BMB Reports
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• 제44권5호
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• pp.291-297
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• 2011

Aging is one of the most complicated biological processes in all species. A number of different model organisms from yeast to monkeys have been studied to understand the aging process. Until recently, many different age-related genes and age-regulating cellular pathways, such as insulin/IGF-1-like signal, mitochondrial dysfunction, Sir2 pathway, have been identified through classical genetic studies. Parallel to genetic approaches, genome-wide approaches have provided valuable insights for the understanding of molecular mechanisms occurring during aging. Gene expression profiling analysis can measure the transcriptional alteration of multiple genes in a genome simultaneously and is widely used to elucidate the mechanisms of complex biological pathways. Here, current global gene expression profiling studies on normal aging and age-related genetic/environmental interventions in widely-used model organisms are briefly reviewed.

• Biotechnology and Bioprocess Engineering:BBE
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• 제2권2호
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• pp.69-72
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• 1997

The pH of microbial culture medium was calculated from equations of equilibrium, meterial balances for ionic components and electro-neutrality theory. Ammonium ion consumption and Acetic acid production are found out to be the major contributors for the alteration of the pH as well as the buffer capacity of the medium. By measuring the buffer capacity on-line, levels of acetic acid were estimated by a software sensor using pH signal in a fermentation process of E.coli growing in a minimal medium. The measured values of acetic acid showed good correlation to those of estimated by the software sensor.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1998년도 추계학술대회
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• pp.187-188
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• 1998

A low cost, low power, portable cardiac event recorder as a tether-free biological signal processor was developed. Dual channel ECG signals are sampled at 128Hz in 12 bits resolution. Sampled data are continuously recorded in a circular buffer. If event button is pressed, 2 minutes data before and after the event are recorded in 512 Kbyte SRAM. Total 11 events can be recorded. Data can be transferred to PC through RS-232 protocol. It operates for two months by a half AA size 3.6V Lithium battery. The system size is $55\times55\times13[mm^3]$.