• BMB Reports
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• 제33권5호
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• pp.370-378
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• 2000

Incubation of purified laminin1-nidogen1 complexes with $[{\gamma}-^{32}P]-ATP$ in the presence of the catalytic subunit of the protein kinase A (cAMP-dependent protein kinase) resulted in the phosphorylation of the alpha chain of laminin-1 and of the nidogen-1 molecule. Aminoacid electrophoresis indicated that phosphate was incorporated on serine residues. The phosphorylation effect of laminin-1 on the process of self assembly was studied by turbidometry. In these experiments, the phosphorylated laminin-1 showed a reduced maximal aggregation capacity in comparison to the non-phosphorylated molecule. Examination of the laminin-1 network under the electron microscope showed that the phosphorylated sample formed mainly linear extended oligomers, in contrast to controls that formed large and dense multimeric aggregates. Heparin binding on phosphorylated laminin-1 in comparison to controls was also tested using solid-phase binding assays. The results indicated an enhanced heparin binding to the phosphorylated protein. The results of this study indicate that laminin1-nidogen1 is a substrate for protein kinase A in vitro. This phosphorylation had an obvious influence on the lamininl-nidogen1 network formation and the heparin binding capacity of this molecule. However, further studies are needed to investigate whether or not this phenomenon could play a role in the formation of the structure of basement membranes in vivo.

• 한국토양비료학회지
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• 제32권1호
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• pp.84-89
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• 1999

우리나라 시설재배지토양(施設栽培地土壤)에서 채취(採取)된 72개(個) 밭 토양(土壤)에 대해 Jew's mallow(Corchorus olitorius, L.)의 유식물(幼植物)을 이용(利用)하여 토양(土壤)의 미생물성(微生物性) 간역(簡易) 평가방법(評價方法)을 시험(試驗)하였다. 검정토양(檢定土壤)의 이화학성(理化學性) 차이(差異)의 영향(影響)을 적게 하기 위해 각각의 검정토양(檢定土壤)에 이화학성(理化學性)의 문제(問題)가 없는 살균토괴(殺菌土壞)을 공통적(共通的)으로 4배량(倍量) 가(加)하여 혼합(混合)하였다. 이때, 2적(適) 배양(培養)한 혼합토괴(混合土壞)의 미생물성(微生物性)은 혼합전(混合前)의 미생물성(微生物成)을 반영(反影)하고 있다고 가정(假定)하였다. 1포트에 250g의 혼합토(混合土)를 넣고 Jew's mallow (Corchorusd olitorius L.)을 파종(播種)하여 4주간(週間) 재배(栽培)한 후(後)의 지상부(地上部)의 생육상(生育相)으로 부터 토양(土壤)의 미생물성(微生物性)을 평가(評價)한 결과(結果), 72토양내(土壤內) EC가 $5dS\;m^{-1}$ 이상(以上)의 18토양(土壤)에서는 미생물성(微生物性)이 악화(惡化)될 수 있는 가능성(可能性)이 나타났다. 또한 EC가 $5dS\;m^{-1}$ 미만(未滿)일 때도 미생물성(微生物性)이 악화(惡化)될 수 있는 가능성(可能性)이 있는 토양(土壤)이 존재(存在)하는 한편, 미생물성(微生物性)이 양호(良好)한 토양(土壤)이 존재(存在)할 가능성(可能性)도 보였다. 이 방법(方法)은 Bioassay이므로, 미생물(微生物)의 수(數)나 활성(活性)을 측정(測定)할 필요(必要)는 없다.

• 분석과학
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• 제13권2호
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• pp.136-150
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• 2000

생체시료에서 미지 의약품의 검출을 위하여, 민감하고 선택적인 박층크로마토그래피인 자동화 점적기 및 자외선 스펙트럼 검출기를 장착한 고성능박층크로마토그라피(HPTLC)를 시도하였다. 205종의 의약품에 대한 Rf값과 자외선흡수스펙트럼(scan 200-360 nm)을 이용하여 HPTLC 데이터베이스를 구축하였으며, 또한 96종의 의약품에 대하여는 리도카인에 대한 상대적 머무름시간(relative retention time; RRT) 및 질량스펙트럼을 이용하여 GC/MS 데이터베이스를 구축하였다. 생체시료중 의약품성분은 고상추출법(Clean Screen ZSDAU020)으로 추출한 후, 이들 시료들을 HPTLC 및 GC/MS 데이터베이스에 적용시켜 검색한 결과 90% 이상의 매우 양호한 일치율로 검색할 수 있었으며, 이들 의약품들을 GC/MS로 확인하였다. 결론적으로, 우리가 구축한 HPTLC 및 GC/MS 데이터베이스체계는 생체시료에서 미지의 의약품성분을 검색하는데 매우 유용한 방법으로 사료된다.

• Journal of Biosystems Engineering
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• 제24권3호
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• pp.243-252
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• 1999

The damage caused in the processes and distributions after harvesting the fruits and vegetables is attributed to the mechanical factors such as compressive and impact forces. Compression tests of biological materials provide an objective method for determining the apparent modulus of elasticity and mechanical properties which are significant in quality evaluation and control, and them maximum allowable compressive forces for minimizing mechanical damage. This study was performed to determine the mechanical properties of apple and pear, and to investigate effect of specimen shapes on the mechanical properties of them. A computer program was developed for measuring the mechanical properties and analyzing the data obtained from the measurement. Compression tests on the sample were performed with then replications at each treatment and at 25 mm/min loading rate. Mechanical properties of the apple was generally shown the higher value than those of the pear, and it was though that data obtained form the cylindrical specimen removed from the sample was more reliable than from the specimen cut in half.

• 한국지능시스템학회논문지
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• 제8권5호
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• pp.61-71
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• 1998

Genetic Algorithm(GA) is a method of approaching optimization problems by modeling and simulating the biological evolution. GA needs large time-consuming, so ti had better do on a parallel computer architecture. Our proposed system has a VLSI-oriented interconnection network, which is constructed from a viewpoint of fractal geometry, so that self-similarity is considered in its configuration. The approach to Parallel Genetic Algorithm(PGA) on our proposed system is explained, and then, we construct the classifier system such that the set of samples is classified into weveral classes based on the features of each sample. In the process of designing the classifier system, We have applied PGA to the Traveling Salesman Problem and classified the sample set in the Euclidean space into several categories with a measure of the distance.

• 대한가정학회지
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• 제40권1호
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• pp.179-194
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• 2002

This study investigates the incidence and risk factors of adolescents' unwanted sexual behavior for a sample of 769 adolescent males and females. Seven percent of the sample experienced unwanted sexual behavior. Female adolescents were more likely to be victims of unwanted sexual behavior Adolescents with an unwanted sexual behavior, as compared to those without, were more likely to have experienced pregnancy, to drink alcohol, to be sensitive to romantic circumstances, to love his/her parker, to have fathers with higher education, to have lower family income, to have friends with similar norms and attitudes, to be more experienced sexually, not to have both biological parent, to have parents who did not monitor their behavior closet, to get partner's pressure to get sexually involved, and to have higher devotion level toward parker or their relationship.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1998년도 추계학술대회
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• pp.78-79
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• 1998

This paper describes a real time algorithm for blood flow estimation of LDF(laser Doppler flowmeter). Many algorithms for blood flow estimation are using power spectral density of Doppler signal by blood flow. In these research, the fast Fourier transformation is used to estimate power spectral density. This is a block processing procedure rather than real time processing. The algorithm in this paper used parametric spectral estimation. This has real time capability by estimation of AR(autoregressive) parameters sample by sample, and has smoothing power spectrum. Also, the frequency resolution is not limited by number of samples used to estimate AR parameter. Another advantage of this algorithm is that AR model enhance SNR.

• 대한화학회지
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• 제19권3호
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• pp.163-168
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• 1975

쌀, 생선 및 표준 기준 배추시료 속에 함유된 수은의 함량을 두 개의 독립적인 분석 방법 즉, 과거 본 연구실에서 개발한 방법과 Sjostrand 방법에 의하여 분석하였다. 분석 결과는 시료의 종류에 따라서 서로 다른 매트릭스 효과를 보였다. 예를 들면 배추과 생선 시료는 같은 매티릭스 효과를 나타내었으나 쌀 시료는 이 두 종의 시료과 비교하여 다른 효과를 보였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 하계학술대회 논문집 B
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• pp.661-663
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• 1995

Chromosome analysis is an important and difficult task for clinical diagnosis for mutagen dosimetry, and for biological research. It is expensive, time consuming and imprecise when performed manually. Efforts to automate some or all of the procedures have continued for more than 30 years, with only limited success. An acquiring sample from chromosome group is not solved with automatic method. It is still performed by user. This paper represents the method of an automatic chromosome sample extraction which based on region splitting, and scan converted method.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 추계학술대회
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• pp.251-253
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• 1996

Noninvasive monitoring of total Hemoglobin value is feasible with the use of spectroscopic measurements. As a step toward tile final goal of the development of a noninvasive monitor, the spectra$(400{\sim}800mm)$of EDTA whole blood were obtained along with reference total Hemoglobin values. Under the same condition water spectrum was generated. It was subtracted from each blood sample, and then tile first derivative of each subtracted data was taken by 'approximated first derivative algorithm' with gap (1,6,10,20nm). The correlation was obtained between total Hemoglobin and first valley wavelength of first derivative spectrum (sample number: 93).