• 대한약침학회지
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• 제16권2호
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• pp.55-61
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• 2013

Objective: Ulmi Pumilae Cortex (UPC) is a deciduous tree with uneven pinnate leaves and is classified as a subfamily of Ulmuceae and contains many pharmacologically active constituents. The aim of this study was to investigate the effects of UPC on the growth and survival of AGS cells, the most common human gastric adenocarcinoma cell lines. Methods: The AGS cells were treated with varying concentrations of UPC. Analyses of the sub G1, caspase-3 activity, and mitochondrial depolarization were conducted to determine whether AGS cell death occured by apoptosis. Furthermore, to identify the role of the transient receptor potential melastatin (TRPM) 7 channels in AGS cell growth and survival, we used human embryonic kidney (HEK) 293 cells overexpressed with TRPM7 channels. Results: The addition of UPC to a culture medium inhibited AGS cell growth and survival. Experimental results showed that the sub G1, caspase-3 activity, and mitochondrial depolarization were increased. Furthermore, TRPM7 channel overexpression in HEK 293 cells exacerbated UPC-induced cell death. Conclusion: These findings indicate that UPC inhibits the growth and survival of gastric cancer cells due to a blockade of the TRPM7 channel activity. Therefore, UPC is a potential drug for treatment of gastric cancer, and TRPM7 channels may play an important role in survival in cases of gastric cancer.

• 대한예방한의학회지
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• 제18권2호
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• pp.81-88
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• 2014

Objective : This study is a survey research that investigates the kinds of medicinal herbs actually used in Yaksun(medicianal food) restaurants, the frequency and the way of using herbs in Yaksun. Through this study, we assumed that it will be used basis data on further Korean Yaksun research. Method : We conducted survey targeting for 26 Yaksun restaurants and Temple food restaurants serving Yaksun cuisine(medicinal food) menu from July 2012 to January 2013. The questionnaire was composed of several parts including the kinds of medicinal herbs that was used in Yaksun, medicinal food types that use a lot of medicinal herbs, medicinal herbs criteria used in the selection of medicinal food, and education experience learning Yaksun cuisine. Results : Only 11 restaurants answered the questionnaire among the targeting restaurants of survey objects. The number of Herbs was investigated in each restaurant was maximum 65 kinds and minimum 7 kinds (average 32 kinds). All restaurants used Angelicae Gigantis Radix in their restaurant. And Nelumbinis Semen, Zingiberis Rhizoma, Glycyrrhizae Radix, Acanthopanacis Cortex, and Gardeniae Fructus are well used medicinal herbs in Yaksun. Types of medicinal food using a lot of herbs were rices porridges rice cakes, both vegetables salads and stews soups. Almost chefs or restaurant's representatives learned cooking medicinal food at temples, food research centers, university attached institutions, and cooking schools. Conclusion : Medicinal herbs used in Yaksun restaurants are familiar with Korean and easily available. These herbs has better efficacy, taste, scent, color in comparison of the others. For the development of Korean Yaksun, further research of divers parts in Yaksun materials should be conducted.

• 한국한의학연구원논문집
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• 제18권2호
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• pp.55-64
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• 2012

Objectives: This study was performed to review the use of statistical analysis methods for the Traditional Korean Medicine studies listed on the Korea Citation Index from 1999 to 2008. Methods: A total of 4217 studies published on four journals of Traditional Korean Medicine were screened and 2682 articles using statistical methods were selected for the review. The selected studies were analysed according to their published year, statistical method and statistical package for use. Results: Statistical methods were used steadily in 64.6% of the articles after 2001, the most used statistical methods(57%) were mean difference comparison between 2 groups. The number of statistical methods mostly used in one article was identified as one in 1931 articles (72.0%). Duncan (36.8%) and Tukey (26.5%) were used for the ANOVA post hoc analysis. SPSS was most frequently used 68% out of Statistical package programs.(the number of mean difference comparison among more than 3 groups was continuously increasing and that makes post hoc being used. skills of statistical methods need to be diversified.) Conclusion: The interest on the proper use of statistical analysis in the research is increasing. This study will contribute to the Evidence-based Teaching on research methodology in Traditional Korean Medicine.

• 대한한의진단학회지
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• 제9권1호
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• pp.69-83
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• 2005

Background and purpose: The purpose of this study is to review the recent achievements on ryodoraku and suggest new ryodoraku studies. Methods: The study papers related with ryodoraku, published in foreign countries and Korea up to the present, were collected first by internet search & journal. And then the collected papers were classified and summarized. Results and Conclusion: There are three study trends. One is finding some effect and analysis for some symptoms, another is finding a distinguishing mark and a diagnostic index, and the other is raising several points & providing solution & studying about relativity with another diagnosis. Some studies finding some effect and analysis for some symptoms are to be classified into four items(1. effect of drugs medication. 2. effect of ryodoraku therapy. 3. effect of other treatments. 4. effect of other treatments with ryodoraku therapy). Other studies finding a distinguishing mark and a diagnostic index are to be classified into two items(1. diagnostic index of symptoms. 2. distinguishing mark of disease). The other studies are to be classified into three items(1. ryodoraku introduction and raising several points at issue. 2. improvement machinery and tools. 3. studying about relativity with another diagnosis). Finally we need solving the ryodoraku problems(the condition of measurement and reproducibility, relation with Kyung-rak(經絡) and Ryodoraku, the reason of Ryodoraku points producing and etc.).

• 대한한의진단학회지
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• 제15권3호
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• pp.235-244
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• 2011

Objectives: There are four types of diagnostic methods in Oriental medicine, and visual inspection is the first method among them. This study was written in order to complement further understanding on visual inspection. Methods: The authors reviewed a word related with visual inspection in Nei-Ching. The authors researched static words such as bigger/smaller, longer/shorter, slower/faster, curved/straight, one-sided/fair, and groove/uplift, and active words such as extension and contraction, shake, tremor, slow, fast, walk, run, standing, lying, and sitting related with visual inspection in Nei-Ching. Results: The static words linked with visual inspection are related with skin, muscles, fat, and especially the liver, stomach, and large intestine. The active words linked with visual inspection are related with movement of muscles, fat, and bone. Conclusion: In this study, the authors provided further understanding on visual inspection in Nei-Ching. However, there was no clear reference point about appearances and movements.

• 대한한의진단학회지
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• 제15권3호
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• pp.223-234
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• 2011

Objectives: This study shows feasibility and suitability of a microelectromechanical system inertial measurement unit(MEMS-IMU) as a helpful measurement device for evaluating movement system impairment syndrome. Methods: We reviewed articles of two fields in this study. First, we reviewed articles about movement system impairment syndrome(MSIS) as a brand new viewpoint of diagnosing and treating musculoskeletal pain. Second, we reviewed articles about conventional motion analysis system and inertial measurement unit(IMU) to show the superiority of IMU in analyzing the human movement. All papers were searched by SciVerse, world largest search engine and database about many academic fields including engineering and medicine. Results: Some physical quantities of human motions can be useful to the diagnosis of MSIS, and those data can be obtained by the MEMS-IMU without the weak points of the conventional motion analysis systems. Conclusions: Using MEMS-IMU as a measurement unit for diagnosing and evaluating MSIS is feasible and can be extended to many further studies.

• 대한약침학회지
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• 제19권2호
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• pp.129-136
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• 2016

Objectives: The crude extracts of Scutellaria barbata D. Don (SB) have traditionally demonstrated inhibitory effects on numerous human cancers both in vitro and in vivo. Gastric cancer is one of the most common types of cancer on world. The authors investigated the effects of an ethanol extract of Scutellaria barbata D. Don (ESB) on the growth and survival of MKN-45 cells (a human gastric adenocarcinoma cell line). Methods: The MKN-45 cells were treated with different concentrations of ESB, and cell death was examined using an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Analyses of sub-G1 peaks, caspase-3 and -9 activities, and mitochondrial membrane depolarizations were conducted to determine the anti-cancer effects of SB on MKN-45 cells. Also, intracellular reactive oxygen species (ROS) generation was investigated. Results: ESB inhibited the growth of MKN-45 cells, caused cell cycle arrest, and increased the sub-G1 population. In addition, ESB markedly increased mitochondrial membrane depolarization and the activities of caspase-3 and -9. ESB exerted anti-proliferative effects on MKN-45 cells by modulating the mitogen-activated protein kinase (MAPK) signaling pathway and by increasing the generation of ROS. Furthermore, combinations of anti-cancer drugs plus ESB suppressed cell growth more than treatments with an agent or ESB, and this was especially true for cisplatin, etoposide, and doxorubicin. Conclusion: ESB has a dose-dependent cytotoxic effect on MKN-45 cells and this is closely associated with the induction of apoptosis. ESB-induced apoptosis is mediated by mitochondria-, caspase- and MAPK dependent pathways. In addition, ESB enhances ROS generation and increases the chemosensitivity of MKN-45 cells. These results suggest that treatment with ESB can inhibit the proliferation and promote the apoptosis of human gastric adenocarcinoma cells by modulating the caspase-, MAPK- and ROS-dependent pathway.

• 대한약침학회지
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• 제16권3호
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• pp.39-45
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• 2013

Objectives: Buxus Microphylla var. Koreana Nakai Extract (BMKNE) is used as a folk remedy for malaria and veneral disease. In the present study, we investigated the effects of BMKNE in the growth and the survival of AGS cells, the most common human gastric adenocarcinoma cell lines. Methods: The AGS cells were treated with varying concentrations of BMKNE. Analyses of the sub G1 peak, the caspase-3 and -9 activities, and the mitochondrial depolarization were conducted to determine whether AGS cell death occured by apoptosis. Also, to identify the role of transient receptor potential melastatin (TRPM) 7 channels in AGS cell growth and survival, we used human embryonic kidney (HEK) 293 cells overexpressed with TRPM7 channels. Results: Experimental results showed that the sub G1 peak, the caspase-3 and -9 activities, and the mitochondrial depolarization were increased. Therefore, BMKNE was found to induce the apoptosis of these cells, and this apoptosis was inhibited by SB203580 (a p38 mitogen-activated protein kinase (MAPK) inhibitor), and by a c-jun NH2-terminal kinase (JNK) II inhibitor. Furthermore, BMKNE inhibited TRPM7 currents and TRPM7 channel over-expressions in HEK 293 cells, exacerbating BMKNE-induced cell death. Conclusions: These findings indicate that BMKNE inhibits the growth and the survival of gastric cancer cells due to a blockade of the TRPM7 channel's activity and MAPK signaling. Therefore, BMKNE is a potential drug for treatment of gastric cancer, and both the TRPM7 channel and MAPK signaling may play an important role in survival in gastric cancer cells.

• 한국한의학연구원논문집
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• 제18권1호
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• pp.85-92
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• 2012

Objective : The purpose of this study was to investigate the anti-cancer effects of Sophorae Radix and the effects of 5-Fluorouracil (5-FU) in human gastric adenocarcinoma cells (AGS). Method : We used human gastric adenocarcinoma cell line, AGS cells. We examined cell death by MTT assay and caspase 3 assay with Sophorae Radix. To examine the inhibitory effects of Sophorae Radix, cell cycle (sub G1) analysis was done the AGS cells after three days with Sophorae Radix. The reversibility of Sophorae Radix was examined on one day to five days treatment with 100 ${\mu}g/ml$ Sophorae Radix. Result : Sophorae Radix inhibited the growth of AGS cells in a dose-dependent fashion. Also we showed that Sophorae Radix induced apoptosis in AGS cells by MTT assay, caspase 3 assay and sub-G1 analysis. Sophorae Radix combined with 5-FU markedly inhibited the growth of AGS cells compared to Sophorae Radix or 5-FU alone. After 3 days treatment of AGS cells with Sophorae Radix, the fraction of cells in sub-G1 phase was much higher than that of the control group. Conclusion : Our findings provide insight into unraveling the effects of Sophorae Radix in human gastric adenocarcinoma cells and developing therapeutic agents against gastric cancer.

• 동의생리병리학회지
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• 제23권4호
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• pp.765-771
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• 2009

The medical informatics for clustering Sasang types with collected clinical data is important for the personalized medicine, but it has not been thoroughly studied yet. The purpose of this study was to examine the usefulness of neural network data mining algorithm for traditional Korean medicine. We used Kohonen neural network, the Self-Organizing Map (SOM), for the analysis of biomedical information following data pre-processing and calculated the validity index as percentage correctly predicted and type-specific sensitivity. We can extract 12 data fields from 30 after data pre-processing with correlation analysis and latent functional relationship analysis. The profile of Myers-Briggs Type Inidcator and Bio-Impedance Analysis data which are clustered with SOM was similar to that of original measurements. The percentage correctly predicted was 56%, and sensitivity for So-Yang, Tae-Eum and So-Eum type were 56%, 48%, and 61%, respectively. This study showed that the neural network algorithm for clustering Sasang types based on clinical data is useful for the sasang type diagnostic test itself. We discussed the importance of data pre-processing and clustering algorithm for the validity of medical devices in traditional Korean medicine.