• Journal of The Korean Society of Agricultural Engineers
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• v.54 no.1
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• pp.63-72
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• 2012

In livestock industry, damage caused by the epidemic diseases such as Foot-and-Mouth Disease (FMD), Highly-Pathogenic-Avian-Influenza (HPAI) and Porcine-Reproductive-and-Respiratory-Syndrome (PRRS) was very serious. The financial loss incurred from FMD alone which occurred on Nov. 2011 in Korea was estimated at 3 billion won, 23 % of annual livestock industry production. The livestock industry in Korea has greater risk of disease infection because of high density production, etc. Investigating the spread of livestock diseases should consider both direct and indirect contact as well as other various factors including airborne. Airborne infection of livestock disease was first hypothesised in the early 1900s, however, field experimental studies are still limited. Furthermore, no protocol is available in detecting airborne viruses in the field. In this study, effective virus samplers were investigated by comparative analysis of the type of samplers used detect to airborne virus. Laboratory experiments were conducted to compare virus samplers such as Bio-sampler, Dust-sampler, Compact-Cascade-Impactor (CCI) and Microflow in detecting PRRSV. Samples were analyzed by Reverse-Transcription PCR to assess the efficiency of the instrument in detecting the airborne virus. First, samples were classified into five levels according to light intensity of gel images and then the classified results were normalized. In every case, Bio-sampler and Dust-sampler were comparable with each other and have shown to be more effective than CCI and Microflow samplers.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.26 no.3
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• pp.324-333
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• 2016

Objectives: We aimed to compare the sampling performance of different flow-based impactor samplers for collecting fungal spores and bacteria and to explore the association of the level of bioaerosols with activity patterns of occupants in daycare center settings. Methods: For comparison of sampling performance, two different flow-based samplers (greater than 100 L/min or not) were selected; a low flow-based sampler (one-stage Andersen sampler) and two high flow-based samplers (DUO SAS SUPER 360 sampler, BUCK bio-culture sampler). We collected airborne mold and bacteria in 30 daycare centers with various levels of contaminated air. Three repeat samplings per each sampler were performed. Mold and bacteria were grown for 96 hours at $25{\pm}1^{\circ}C$ and 48 hours at $35{\pm}1^{\circ}C$, respectively. The Andersen and SAS samplers were used for investigating the association between the level of bioaerosols and the activity patterns of occupants in daycares. Particular matters 10($PM_{10}$), temperature, and relative humidity were monitored as well. Samplings were carried out with one-hour interval from 9 to 5 O'clock. For statistical comparisons, Kruskal-Wallis test, Wilcoxon's signed rank test, and multiple regression analysis were carried out. Results: The airborne level of molds by the low flow-based sampler were significantly higher than that of high flow-based samplers (indoor, P=0.037; outdoor, P=0.041). However, no statistical difference was observed in the airborne level of bacteria by each sampler. Also the level of bioaerosols varied by the time, particularly with different activity patterns in daycare centers. The higher level of mold and bacteria were observed in play time in indoor. Similarly, the concentrations of $PM_{10}$ were significantly associated with the level of bioaerosols (P<0.05). Conclusions: Our findings indicate that the flow rate of sampler, rather than total air volume, could be able to affect the results of sampling. Also, the level of airborne mold and bacteria vary behavior patterns of occupants in indoor of daycare settings. Therefore, different samplers with other flow rate may be selected for mold or bacteria sampling, and activity patterns should be considered for bioaerosol sampling as well.

• Journal of Environmental Science International
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• v.23 no.12
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• pp.1963-1970
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• 2014

Ambient particulate matters($PM_{10}$ and $PM_{2.5}$) were investigated at GNTECH university in Jinju city. Samples were collected using a dichotomous sampler(series 240, Andersen Corp.) and a TEOM(Tapered Element Oscillating Microbalance) monitor period from November 2012 to October 2013. For the dichotomous sampler measurements, daily 24-h integrated $PM_{2.5}$ and $PM_{10-2.5}$ ambient air samples were collected at a total flow rate of 16.7 L /min. For the TEOM monitor measurements, daily 1-h integrated $PM_{10}$ ambient air samples were collected at a flow rate of 16.7 L /min. The annual average concentrations of $PM_{10-2.5}$ and $PM_{2.5}$ by a dichotomous sampler were $10.0{\pm}6.1{\mu}g/m^3$ and $22.6{\pm}9.3{\mu}g/m^3$, respectively. And $PM_{10}$ concentration by dichotomous sampler were similar to TEOM monitor by $32.7{\pm}12.9{\mu}g/m^3$ and $31.7{\pm}11.3{\mu}g/m^3$, respectively. And good correlation ($R^2=0.964$) between the two methods was observed. The annual average of $PM_{2.5}/PM_{10}$ ratio was $0.70{\pm}0.12$.

• Journal of environmental and Sanitary engineering
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• v.24 no.4
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• pp.102-111
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• 2009

In this study, Particulate carbon were determined from 1990 to 1995. The annual variation were investigated. The sampling was carried out using high volume air sampler. Average concentrations of EC and OC during the yellow sand event were $25.70{\mu}g/m^3$ and $13.91{\mu}g/m^3$, respectively, $22.10{\mu}g/m^3$ and $10.33{\mu}g/m^3$ during the non-yellow sand event. TC concentration of TSP were 10.7% during the yellow sand event and 20.6% during the non-yellow sand event. Average concentration rate of EC and OC of TC were 64.9% and 35.1%, respectively during the yellow sand event, 67.6% and 32.4% during the non-yellow sand event.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.3102-3107
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• 2008

This paper was focused on the particulate matter (PM) on the gasoline and bio ethanol. Bio ethanol as a clean fuel is considered one of the alternative fuels that decreased the PM emission from the vehicle. Particle formation in SI engine was depended on the fuel and engine operating condition. In this paper, Particle number concentration behaviors were analyzed by DMS500 (Differential Mobility Spectrometer) and CPC (Condensation Particle Counter) instrument which was recommended by PMP (Particle Measurement Programme). Particle emissions were measured with various engine operating variables such as air excess ratio ($\lambda$), spark timing and intake valve opening (IVO) at part load condition. In vehicle test, the number of particulate matter was analyzed with golden particle measurement system, which was consist of CVS (Constant Volume Sampler), particle number counter and particle number diluter.

• Korean Journal of Agricultural Science
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• v.49 no.2
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• pp.239-247
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• 2022

The physico-chemical properties of agricultural soil are factors that affect crop productivity and soil fertility. In Korea, agricultural environment change surveys have been conducted every four years since 1999. The purpose of this study is to investigate the status and changes in the physico-chemical properties of agricultural soil in Chungcheongnam-do. Samples were collected from the exact location of the aforementioned environment survey, and environmental variables (land use, type of crop cultivated) were investigated in relation to the samples. Soil collection was conducted using a core sampler and a single gouge auger. The bulk density of the soil generally increased up to a depth of sampling of 40 cm but decreased thereafter to a depth of 60 cm. Additionally, the bulk density was highest in the upland soil case and stood at 1.59 g·cm^-3, while the lowest value of 1.52 g·cm^-3 was obtained from orchard soil samples. Conversely, the porosity and moisture gradually decreased at soil depths of 0 - 40 cm and increased at depths of 40 - 60 cm. Most of the soil chemical properties generally decreased with an increase the soil depth from 0 to 70 cm, but electrical conductivity (EC) increased up to a depth of 40 cm. Therefore, it is judged that it is necessary to lower the bulk density by supplying organic matter for agricultural land in Chungcheongnam-do.

• Journal of odor and indoor environment
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• v.16 no.1
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• pp.47-53
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• 2017

Mold is one of the important bio-aerosols affecting human health in the indoor environment. To manage mold contamination, it is necessary to use an appropriate method for its detection and enumeration. Recently, the impaction method of ISO 16000-18 has been established as one of methods to detect and enumerate molds in air. To investigate the general use of the impaction method for mold detection in domestic indoor environments, the suitability of the method was assessed using different antibiotics, media and air samplers. All of the three antibiotics tested - ampicillin, chloramphenicol and streptomycin - showed inhibitory effects on bacterial colony formation on MEA and DG-18 media, without inhibiting mold growth. Of these three antibiotics, ampicillin was the most effective. There was no statistical difference between MEA and DG-18 media in the measurement of mold concentration. The formation of discriminative colony morphology was more apparent in DG-18 media. No significant difference in the measurement of mold concentration was found between Andersen samplers and MAS-100NT samplers, which are two major samplers introduced in Korea.

• Proceedings of the Korean Environmental Health Society Conference
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• 2005.06a
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• pp.273-278
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• 2005

This study was performed to investigate the characteristics of distribution for airborne bio aerosol in 11 public facilities in Seoul from June to July. The collected samples are total suspended bacteria in indoor and outdoor Anderson six stage air sampler by the IAQ standard method of Ministry of Environment in Korea. The concentration of total suspended bacteria in the theater higher than IAQ standards. As the results of the survey, the most high indoor air mean concentration of bacteria $1273CFU/m^3$ was theater and the most high outdoor air mean concentration of bacteria $^229 CFU/m3$was Kindergarten. The mean concentration of bacteria in the theater was higher than the IAQ standards established by the Ministry of Environment, Republic of Korea. Moreover, this study was for investigation a part of indoor air pollution condition in public facilities. It means that this study can't represent fDr all of public facilities. Therefore, we suggest that long and middle term country plan for management of IAQ should be established through long-term and continuous investigation of IAQ condition. Also above consideration in mind, it is suggest that the research for source contribution of the results on these need further study.

• Journal of Korean Society of Rural Planning
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• v.19 no.4
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• pp.137-147
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• 2013

Most of livestock houses are concentrated in certain area with mass rearing system resulting in rapid spread of infectious diseases such as HPAI (highly pathogenic avian influenza). The livestock-related vehicles which frequently travel between farms could be a major factor for disease spread by means of transmission of airborne aerosol including pathogens. This study was focused on the quantitative measurement of aerosol concentration by field experiment while vehicles were passing through the road. The TSP (total suspended particle) and PM10 (particle matter) were measured using air sampler with teflon filter installed downward the road with consideration of weather forecast and the direction of road. And aerosol spectrometer and video recorders were also used to measure the real-time distribution of aerosol concentration by its size. The results showed that PM2.5 was not considerable for transmission of airborne aerosol from the livestock-related vehicle. The mass generated from the road during the vehicle movement was measured and calculated to 241.4 ${\mu}g/m^3$ by means of the difference between TSP and PM2.5. The dispersion distance was predicted by 79.6 m from the trend curve.

• Journal of agriculture & life science
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• v.45 no.3
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• pp.89-96
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• 2011

The objective of this study is to evaluate reduction efficiency of various air purifiers on airborne microorganism causing air infected animal disease according to sample collection method. Collection efficiencies of MS2 virus and Pseudomonas fluorescens by biosampler was significantly higher than those by button sampler (p<0.05). Regardless of types of air purifier and sample collection method, temporal reduction efficiencies of MS2 virus and P. fluorescens compared to initial background concentration were >50% and >45% on 5 minutes, >70% and >50% on 15 minutes, >80% and >70% on 30 minutes and >90% and >75% on 60 minutes after operating air purifier, respectively. The air purifier of ionizer type showed the highest reduction efficiency on MS2 virus followed by air purifier of electronic precipitation, water filter and dry filter while the reduction efficiency of air purifier on P. fluorescens was highest in the electronic precipitation type followed by ionizer type, dry filter type and water filter type (p<0.05). Based on the results obtained from this study, temporal reduction efficiency of air purifier on MS2 virus was relatively higher than P. fluorescens.